Reviewed, UniProtKB/Swiss-Prot Q9X2M2 (BETA_STAXY)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Synonyms:	cudB

Organism

Staphylococcus xylosus

Taxonomic identifier

1288 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Choline dehydrogenase HAMAP MF_00750		PRO_0000205605
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	472	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X2M2-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4D8395EED9E584D7	FASTA	560	62,384
	10 20 30 40 50 60 MKESYDYIII GGGSAGSVLG GRLSEDVSNN VLVLEAGRSD YPWDLLIQMP AALMYPAGNK 70 80 90 100 110 120 LYDWIYETTP EPHMDGRKVG HARGKVLGGS SSINGMIYQR GNPMDYEKWA KPEGMESWDY 130 140 150 160 170 180 AHCLPYFKRL ETTFGSKKGD PYRGHHGPIK LRRGPADNPL FQAFFDAGVE AGYNKTPDVN 190 200 210 220 230 240 GFRQEGFGPF DSQVHNGRRV SASRAYLHPA MKRKNLEVQT RAFVTKLNFE GNKVTGVTFK 250 260 270 280 290 300 KNGKEHTESA KEVILSGGAI NSPQLLQLSG IGDSEHLRSL GIEPRIHLPG VGENFEDHLE 310 320 330 340 350 360 VYVQHACKQP VSMQPSLNKL KMPFIGLQWI LGRKGAAASN HFEGGGFVRS NDDVDYPNLM 370 380 390 400 410 420 FHFLPIAVRY DGTKAPAAHG YQVHVGPMYS NSRGHLKIKS KDPFEKPEFV FNYLSTEEDK 430 440 450 460 470 480 REWVEAIKVA RNILKQKALD PFNGGEISPG PEVQTDEEII EWVKRDGETA LHPSCSCRMG 490 500 510 520 530 540 PASDEMSVVD PETFKVHGME NLRVVDASVM PRTTNGNIHS PVLMMAERAS DIIRGKKPLD 550 560 PQYIDFYRHG VHDKDAGTVK

References

[1]	"The choline-converting pathway in Staphylococcus xylosus C2A: genetic and physiological characterization." Rosenstein R., Futter-Bryniok D., Gotz F. J. Bacteriol. 181:2273-2278(1999) [PubMed: 10094709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20267 / Isolate C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF009415 Genomic DNA. Translation: AAD23901.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.99.1. 141621.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_STAXY

Accession

Primary (citable) accession number: Q9X2M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	November 1, 1999
Last modified:	January 20, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)