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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei257ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMAR243274:G1H0Q-963-MONOMER
BRENDAi6.1.1.24 6331

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:TM_1875
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196811 – 487Glutamate--tRNA ligase 2Add BLAST487

Proteomic databases

PRIDEiQ9X2I8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-59231N
IntActiQ9X2I8, 1 interactor
STRINGi243274.TM1875

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 29Combined sources4
Beta strandi34 – 36Combined sources3
Helixi39 – 55Combined sources17
Beta strandi58 – 61Combined sources4
Turni68 – 70Combined sources3
Helixi73 – 85Combined sources13
Beta strandi91 – 93Combined sources3
Turni94 – 96Combined sources3
Helixi105 – 107Combined sources3
Helixi109 – 121Combined sources13
Beta strandi124 – 130Combined sources7
Beta strandi132 – 134Combined sources3
Beta strandi137 – 144Combined sources8
Helixi147 – 151Combined sources5
Beta strandi156 – 160Combined sources5
Beta strandi164 – 171Combined sources8
Turni172 – 174Combined sources3
Beta strandi175 – 180Combined sources6
Helixi181 – 183Combined sources3
Beta strandi187 – 190Combined sources4
Turni192 – 194Combined sources3
Helixi198 – 208Combined sources11
Beta strandi212 – 217Combined sources6
Helixi218 – 223Combined sources6
Helixi224 – 234Combined sources11
Beta strandi240 – 244Combined sources5
Beta strandi252 – 254Combined sources3
Turni257 – 259Combined sources3
Beta strandi261 – 263Combined sources3
Helixi264 – 270Combined sources7
Helixi274 – 283Combined sources10
Helixi296 – 299Combined sources4
Helixi300 – 302Combined sources3
Helixi305 – 307Combined sources3
Helixi317 – 330Combined sources14
Helixi333 – 347Combined sources15
Helixi356 – 366Combined sources11
Turni367 – 369Combined sources3
Helixi373 – 384Combined sources12
Helixi392 – 397Combined sources6
Turni398 – 400Combined sources3
Helixi404 – 416Combined sources13
Helixi423 – 435Combined sources13
Helixi441 – 453Combined sources13
Beta strandi454 – 457Combined sources4
Helixi461 – 468Combined sources8
Helixi470 – 484Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AFHX-ray2.00A2-487[»]
3AKZX-ray2.90A/B/C/D1-487[»]
3AL0X-ray3.37C1-487[»]
ProteinModelPortaliQ9X2I8
SMRiQ9X2I8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X2I8

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi31 – 41"HIGH" regionUniRule annotationAdd BLAST11
Motifi254 – 258"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
InParanoidiQ9X2I8
KOiK01885
OMAiAYYAFDT

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.1160.10, 1 hit
1.10.8.70, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020752 aa-tRNA-synth_I_codon-bd_sub1
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061 Glu_tRNA_lig_a-bdl
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X2I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFITGAFFDI LEVGPKKIRR CFELVRVRFA PSPTGHLHVG GARTALFNWM
60 70 80 90 100
FARKEGGKFI LRIEDTDTER SSREYEQQIL ESLRWCGLDW DEGPDIGGDF
110 120 130 140 150
GPYRQSERLE IYREYAEKLV EDKRAYYVVY DKEDPSKELF TTYEYPHEYK
160 170 180 190 200
EKGHPVTIKF KVLPGKTSFE DLLKGYMEFD NSTLEDFIIM KSNGFPTYNF
210 220 230 240 250
AVVVDDHLMR ISHVFRGEDH LSNTPKQLMI YEAFGWEAPV FMHIPLILGS
260 270 280 290 300
DRTPLSKRHG ATSVEHFRRE GILSRALMNY LALLGWRVEG DEIFTIEEKL
310 320 330 340 350
QSFDPKDISN KGVIFDYQKL EWVNGKHMRR IDLEDLKREF IEWAKYAGKE
360 370 380 390 400
IPSVDERYFS ETLRICREKV NTLSQLYDIM YPFMNDDYEY EKDYVEKFLK
410 420 430 440 450
REEAERVLEE AKKAFKDLNS WNMEEIEKTL RDLSEKGLAS KKVVFQLIRG
460 470 480
AVTGKLVTPG LFETIEVLGK ERTLKRLERT LQFLKKT
Length:487
Mass (Da):57,382
Last modified:November 1, 1999 - v1
Checksum:i5E15A2E9E1580791
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36937.1
PIRiF72200
RefSeqiNP_229671.1, NC_000853.1
WP_010865423.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD36937; AAD36937; TM_1875
GeneIDi897796
KEGGitma:TM1875
PATRICifig|243274.5.peg.1896

Similar proteinsi

Entry informationi

Entry nameiSYE2_THEMA
AccessioniPrimary (citable) accession number: Q9X2I8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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