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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei257ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.24. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:TM_1875
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196811 – 487Glutamate--tRNA ligase 2Add BLAST487

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-59231N.
STRINGi243274.TM1875.

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 29Combined sources4
Beta strandi34 – 36Combined sources3
Helixi39 – 55Combined sources17
Beta strandi58 – 61Combined sources4
Turni68 – 70Combined sources3
Helixi73 – 85Combined sources13
Beta strandi91 – 93Combined sources3
Turni94 – 96Combined sources3
Helixi105 – 107Combined sources3
Helixi109 – 121Combined sources13
Beta strandi124 – 130Combined sources7
Beta strandi132 – 134Combined sources3
Beta strandi137 – 144Combined sources8
Helixi147 – 151Combined sources5
Beta strandi156 – 160Combined sources5
Beta strandi164 – 171Combined sources8
Turni172 – 174Combined sources3
Beta strandi175 – 180Combined sources6
Helixi181 – 183Combined sources3
Beta strandi187 – 190Combined sources4
Turni192 – 194Combined sources3
Helixi198 – 208Combined sources11
Beta strandi212 – 217Combined sources6
Helixi218 – 223Combined sources6
Helixi224 – 234Combined sources11
Beta strandi240 – 244Combined sources5
Beta strandi252 – 254Combined sources3
Turni257 – 259Combined sources3
Beta strandi261 – 263Combined sources3
Helixi264 – 270Combined sources7
Helixi274 – 283Combined sources10
Helixi296 – 299Combined sources4
Helixi300 – 302Combined sources3
Helixi305 – 307Combined sources3
Helixi317 – 330Combined sources14
Helixi333 – 347Combined sources15
Helixi356 – 366Combined sources11
Turni367 – 369Combined sources3
Helixi373 – 384Combined sources12
Helixi392 – 397Combined sources6
Turni398 – 400Combined sources3
Helixi404 – 416Combined sources13
Helixi423 – 435Combined sources13
Helixi441 – 453Combined sources13
Beta strandi454 – 457Combined sources4
Helixi461 – 468Combined sources8
Helixi470 – 484Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AFHX-ray2.00A2-487[»]
3AKZX-ray2.90A/B/C/D1-487[»]
3AL0X-ray3.37C1-487[»]
ProteinModelPortaliQ9X2I8.
SMRiQ9X2I8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X2I8.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi31 – 41"HIGH" regionAdd BLAST11
Motifi254 – 258"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
InParanoidiQ9X2I8.
KOiK01885.
OMAiIDEINDM.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X2I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFITGAFFDI LEVGPKKIRR CFELVRVRFA PSPTGHLHVG GARTALFNWM
60 70 80 90 100
FARKEGGKFI LRIEDTDTER SSREYEQQIL ESLRWCGLDW DEGPDIGGDF
110 120 130 140 150
GPYRQSERLE IYREYAEKLV EDKRAYYVVY DKEDPSKELF TTYEYPHEYK
160 170 180 190 200
EKGHPVTIKF KVLPGKTSFE DLLKGYMEFD NSTLEDFIIM KSNGFPTYNF
210 220 230 240 250
AVVVDDHLMR ISHVFRGEDH LSNTPKQLMI YEAFGWEAPV FMHIPLILGS
260 270 280 290 300
DRTPLSKRHG ATSVEHFRRE GILSRALMNY LALLGWRVEG DEIFTIEEKL
310 320 330 340 350
QSFDPKDISN KGVIFDYQKL EWVNGKHMRR IDLEDLKREF IEWAKYAGKE
360 370 380 390 400
IPSVDERYFS ETLRICREKV NTLSQLYDIM YPFMNDDYEY EKDYVEKFLK
410 420 430 440 450
REEAERVLEE AKKAFKDLNS WNMEEIEKTL RDLSEKGLAS KKVVFQLIRG
460 470 480
AVTGKLVTPG LFETIEVLGK ERTLKRLERT LQFLKKT
Length:487
Mass (Da):57,382
Last modified:November 1, 1999 - v1
Checksum:i5E15A2E9E1580791
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36937.1.
PIRiF72200.
RefSeqiNP_229671.1. NC_000853.1.
WP_010865423.1. NC_000853.1.

Genome annotation databases

EnsemblBacteriaiAAD36937; AAD36937; TM_1875.
GeneIDi897796.
KEGGitma:TM1875.
PATRICi23938739. VBITheMar51294_1896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36937.1.
PIRiF72200.
RefSeqiNP_229671.1. NC_000853.1.
WP_010865423.1. NC_000853.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AFHX-ray2.00A2-487[»]
3AKZX-ray2.90A/B/C/D1-487[»]
3AL0X-ray3.37C1-487[»]
ProteinModelPortaliQ9X2I8.
SMRiQ9X2I8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59231N.
STRINGi243274.TM1875.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36937; AAD36937; TM_1875.
GeneIDi897796.
KEGGitma:TM1875.
PATRICi23938739. VBITheMar51294_1896.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
InParanoidiQ9X2I8.
KOiK01885.
OMAiIDEINDM.

Enzyme and pathway databases

BRENDAi6.1.1.24. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X2I8.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE2_THEMA
AccessioniPrimary (citable) accession number: Q9X2I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.