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Q9X2I8

- SYE2_THEMA

UniProt

Q9X2I8 - SYE2_THEMA

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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei257 – 2571ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1926-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:TM_1875
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Glutamate--tRNA ligase 2PRO_0000119681Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-59231N.
STRINGi243274.TM1875.

Structurei

Secondary structure

1
487
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 294Combined sources
Beta strandi34 – 363Combined sources
Helixi39 – 5517Combined sources
Beta strandi58 – 614Combined sources
Turni68 – 703Combined sources
Helixi73 – 8513Combined sources
Beta strandi91 – 933Combined sources
Turni94 – 963Combined sources
Helixi105 – 1073Combined sources
Helixi109 – 12113Combined sources
Beta strandi124 – 1307Combined sources
Beta strandi132 – 1343Combined sources
Beta strandi137 – 1448Combined sources
Helixi147 – 1515Combined sources
Beta strandi156 – 1605Combined sources
Beta strandi164 – 1718Combined sources
Turni172 – 1743Combined sources
Beta strandi175 – 1806Combined sources
Helixi181 – 1833Combined sources
Beta strandi187 – 1904Combined sources
Turni192 – 1943Combined sources
Helixi198 – 20811Combined sources
Beta strandi212 – 2176Combined sources
Helixi218 – 2236Combined sources
Helixi224 – 23411Combined sources
Beta strandi240 – 2445Combined sources
Beta strandi252 – 2543Combined sources
Turni257 – 2593Combined sources
Beta strandi261 – 2633Combined sources
Helixi264 – 2707Combined sources
Helixi274 – 28310Combined sources
Helixi296 – 2994Combined sources
Helixi300 – 3023Combined sources
Helixi305 – 3073Combined sources
Helixi317 – 33014Combined sources
Helixi333 – 34715Combined sources
Helixi356 – 36611Combined sources
Turni367 – 3693Combined sources
Helixi373 – 38412Combined sources
Helixi392 – 3976Combined sources
Turni398 – 4003Combined sources
Helixi404 – 41613Combined sources
Helixi423 – 43513Combined sources
Helixi441 – 45313Combined sources
Beta strandi454 – 4574Combined sources
Helixi461 – 4688Combined sources
Helixi470 – 48415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AFHX-ray2.00A2-487[»]
3AKZX-ray2.90A/B/C/D1-487[»]
3AL0X-ray3.37C1-487[»]
ProteinModelPortaliQ9X2I8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X2I8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi31 – 4111"HIGH" regionAdd
BLAST
Motifi254 – 2585"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
InParanoidiQ9X2I8.
KOiK01885.
OMAiEIFDMEY.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X2I8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFITGAFFDI LEVGPKKIRR CFELVRVRFA PSPTGHLHVG GARTALFNWM
60 70 80 90 100
FARKEGGKFI LRIEDTDTER SSREYEQQIL ESLRWCGLDW DEGPDIGGDF
110 120 130 140 150
GPYRQSERLE IYREYAEKLV EDKRAYYVVY DKEDPSKELF TTYEYPHEYK
160 170 180 190 200
EKGHPVTIKF KVLPGKTSFE DLLKGYMEFD NSTLEDFIIM KSNGFPTYNF
210 220 230 240 250
AVVVDDHLMR ISHVFRGEDH LSNTPKQLMI YEAFGWEAPV FMHIPLILGS
260 270 280 290 300
DRTPLSKRHG ATSVEHFRRE GILSRALMNY LALLGWRVEG DEIFTIEEKL
310 320 330 340 350
QSFDPKDISN KGVIFDYQKL EWVNGKHMRR IDLEDLKREF IEWAKYAGKE
360 370 380 390 400
IPSVDERYFS ETLRICREKV NTLSQLYDIM YPFMNDDYEY EKDYVEKFLK
410 420 430 440 450
REEAERVLEE AKKAFKDLNS WNMEEIEKTL RDLSEKGLAS KKVVFQLIRG
460 470 480
AVTGKLVTPG LFETIEVLGK ERTLKRLERT LQFLKKT
Length:487
Mass (Da):57,382
Last modified:November 1, 1999 - v1
Checksum:i5E15A2E9E1580791
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36937.1.
PIRiF72200.
RefSeqiNP_229671.1. NC_000853.1.
WP_010865423.1. NC_000853.1.

Genome annotation databases

EnsemblBacteriaiAAD36937; AAD36937; TM_1875.
GeneIDi897796.
KEGGitma:TM1875.
PATRICi23938739. VBITheMar51294_1896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36937.1 .
PIRi F72200.
RefSeqi NP_229671.1. NC_000853.1.
WP_010865423.1. NC_000853.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AFH X-ray 2.00 A 2-487 [» ]
3AKZ X-ray 2.90 A/B/C/D 1-487 [» ]
3AL0 X-ray 3.37 C 1-487 [» ]
ProteinModelPortali Q9X2I8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59231N.
STRINGi 243274.TM1875.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36937 ; AAD36937 ; TM_1875 .
GeneIDi 897796.
KEGGi tma:TM1875.
PATRICi 23938739. VBITheMar51294_1896.

Phylogenomic databases

eggNOGi COG0008.
InParanoidi Q9X2I8.
KOi K01885.
OMAi EIFDMEY.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci TMAR243274:GC6P-1926-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9X2I8.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiSYE2_THEMA
AccessioniPrimary (citable) accession number: Q9X2I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3