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Q9X2I8

- SYE2_THEMA

UniProt

Q9X2I8 - SYE2_THEMA

Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei257 – 2571ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-1926-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetase 2UniRule annotation
    Short name:
    GluRS 2UniRule annotation
    Gene namesi
    Name:gltX2UniRule annotation
    Ordered Locus Names:TM_1875
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487Glutamate--tRNA ligase 2PRO_0000119681Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-59231N.
    STRINGi243274.TM1875.

    Structurei

    Secondary structure

    1
    487
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi26 – 294
    Beta strandi34 – 363
    Helixi39 – 5517
    Beta strandi58 – 614
    Turni68 – 703
    Helixi73 – 8513
    Beta strandi91 – 933
    Turni94 – 963
    Helixi105 – 1073
    Helixi109 – 12113
    Beta strandi124 – 1307
    Beta strandi132 – 1343
    Beta strandi137 – 1448
    Helixi147 – 1515
    Beta strandi156 – 1605
    Beta strandi164 – 1718
    Turni172 – 1743
    Beta strandi175 – 1806
    Helixi181 – 1833
    Beta strandi187 – 1904
    Turni192 – 1943
    Helixi198 – 20811
    Beta strandi212 – 2176
    Helixi218 – 2236
    Helixi224 – 23411
    Beta strandi240 – 2445
    Beta strandi252 – 2543
    Turni257 – 2593
    Beta strandi261 – 2633
    Helixi264 – 2707
    Helixi274 – 28310
    Helixi296 – 2994
    Helixi300 – 3023
    Helixi305 – 3073
    Helixi317 – 33014
    Helixi333 – 34715
    Helixi356 – 36611
    Turni367 – 3693
    Helixi373 – 38412
    Helixi392 – 3976
    Turni398 – 4003
    Helixi404 – 41613
    Helixi423 – 43513
    Helixi441 – 45313
    Beta strandi454 – 4574
    Helixi461 – 4688
    Helixi470 – 48415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AFHX-ray2.00A2-487[»]
    3AKZX-ray2.90A/B/C/D1-487[»]
    3AL0X-ray3.37C1-487[»]
    ProteinModelPortaliQ9X2I8.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X2I8.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi31 – 4111"HIGH" regionAdd
    BLAST
    Motifi254 – 2585"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK01885.
    OMAiEIFDMEY.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X2I8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFITGAFFDI LEVGPKKIRR CFELVRVRFA PSPTGHLHVG GARTALFNWM    50
    FARKEGGKFI LRIEDTDTER SSREYEQQIL ESLRWCGLDW DEGPDIGGDF 100
    GPYRQSERLE IYREYAEKLV EDKRAYYVVY DKEDPSKELF TTYEYPHEYK 150
    EKGHPVTIKF KVLPGKTSFE DLLKGYMEFD NSTLEDFIIM KSNGFPTYNF 200
    AVVVDDHLMR ISHVFRGEDH LSNTPKQLMI YEAFGWEAPV FMHIPLILGS 250
    DRTPLSKRHG ATSVEHFRRE GILSRALMNY LALLGWRVEG DEIFTIEEKL 300
    QSFDPKDISN KGVIFDYQKL EWVNGKHMRR IDLEDLKREF IEWAKYAGKE 350
    IPSVDERYFS ETLRICREKV NTLSQLYDIM YPFMNDDYEY EKDYVEKFLK 400
    REEAERVLEE AKKAFKDLNS WNMEEIEKTL RDLSEKGLAS KKVVFQLIRG 450
    AVTGKLVTPG LFETIEVLGK ERTLKRLERT LQFLKKT 487
    Length:487
    Mass (Da):57,382
    Last modified:November 1, 1999 - v1
    Checksum:i5E15A2E9E1580791
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36937.1.
    PIRiF72200.
    RefSeqiNP_229671.1. NC_000853.1.
    WP_010865423.1. NC_000853.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36937; AAD36937; TM_1875.
    GeneIDi897796.
    KEGGitma:TM1875.
    PATRICi23938739. VBITheMar51294_1896.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36937.1 .
    PIRi F72200.
    RefSeqi NP_229671.1. NC_000853.1.
    WP_010865423.1. NC_000853.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AFH X-ray 2.00 A 2-487 [» ]
    3AKZ X-ray 2.90 A/B/C/D 1-487 [» ]
    3AL0 X-ray 3.37 C 1-487 [» ]
    ProteinModelPortali Q9X2I8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59231N.
    STRINGi 243274.TM1875.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36937 ; AAD36937 ; TM_1875 .
    GeneIDi 897796.
    KEGGi tma:TM1875.
    PATRICi 23938739. VBITheMar51294_1896.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K01885.
    OMAi EIFDMEY.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci TMAR243274:GC6P-1926-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9X2I8.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiSYE2_THEMA
    AccessioniPrimary (citable) accession number: Q9X2I8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3