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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation1 Publication

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation3 Publications

Cofactori

[4Fe-4S] clusterUniRule annotation1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. The two iron atoms in each cluster are connected via a penta-sulfide bridge.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Iron-sulfur (4Fe-4S)UniRule annotation1 Publication1
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1 Publication1
Metal bindingi79Iron-sulfur (4Fe-4S)UniRule annotation1 Publication1
Metal bindingi148Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1 Publication1
Metal bindingi152Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1 Publication1
Metal bindingi155Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.8.4.4. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation3 Publications)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:TM_1862
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003750521 – 430Ribosomal protein S12 methylthiotransferase RimOAdd BLAST430

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1862.

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Helixi11 – 23Combined sources13
Turni24 – 26Combined sources3
Beta strandi34 – 36Combined sources3
Beta strandi39 – 45Combined sources7
Beta strandi48 – 51Combined sources4
Helixi52 – 55Combined sources4
Helixi57 – 64Combined sources8
Turni68 – 70Combined sources3
Beta strandi73 – 78Combined sources6
Helixi79 – 82Combined sources4
Helixi85 – 91Combined sources7
Beta strandi97 – 99Combined sources3
Turni104 – 107Combined sources4
Beta strandi138 – 145Combined sources8
Helixi157 – 159Combined sources3
Helixi169 – 181Combined sources13
Beta strandi186 – 190Combined sources5
Helixi194 – 196Combined sources3
Helixi199 – 202Combined sources4
Helixi207 – 215Combined sources9
Beta strandi217 – 220Combined sources4
Beta strandi222 – 225Combined sources4
Helixi230 – 232Combined sources3
Helixi235 – 243Combined sources9
Beta strandi250 – 252Combined sources3
Helixi260 – 265Combined sources6
Helixi272 – 285Combined sources14
Beta strandi290 – 297Combined sources8
Helixi304 – 317Combined sources14
Beta strandi320 – 326Combined sources7
Turni334 – 337Combined sources4
Helixi344 – 367Combined sources24
Turni368 – 371Combined sources4
Beta strandi373 – 382Combined sources10
Beta strandi385 – 390Combined sources6
Turni395 – 397Combined sources3
Beta strandi401 – 405Combined sources5
Beta strandi412 – 421Combined sources10
Beta strandi424 – 429Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QGQX-ray2.00A/B/C/D/E/F/G/H135-430[»]
4JC0X-ray3.30A/B1-430[»]
ProteinModelPortaliQ9X2H6.
SMRiQ9X2H6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X2H6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 116MTTase N-terminalUniRule annotationAdd BLAST116
Domaini367 – 430TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBM. Bacteria.
COG0621. LUCA.
InParanoidiQ9X2H6.
KOiK14441.
OMAiHYAYPTG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X2H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVGIKVLGC PKNEADCEVL AGVLREGGHE IVFDVKDADV VVLDTCAFIE
60 70 80 90 100
DAKRESIDEI FSFVDAKDQY GYKLVVKGCL VQRYYEELKK EIPEVDQWIG
110 120 130 140 150
VADPEEIANA IENGTDLVPD QPETVYRYRK RIDLEERPYA YVKISDGCDR
160 170 180 190 200
GCTFCSIPSF KGSLRSRSIE DITREVEDLL KEGKKEIILV AQDTTSYGID
210 220 230 240 250
LYRKQALPDL LRRLNSLNGE FWIRVMYLHP DHLTEEIISA MLELDKVVKY
260 270 280 290 300
FDVPVQHGSD KILKLMGRTK SSEELKKMLS SIRERFPDAV LRTSIIVGFP
310 320 330 340 350
GETEEDFEEL KQFVEEIQFD KLGAFVYSDE EGTVAFNLKE KVDPEMAKRR
360 370 380 390 400
QEELLLLQAE ISNSRLDRFV GKKLKFLVEG KEGKFLVGRT WTEAPEVDGV
410 420 430
VFVRGKGKIG DFLEVVIKEH DEYDMWGSVI
Length:430
Mass (Da):49,222
Last modified:November 1, 1999 - v1
Checksum:i345477B796BF3990
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36924.1.
PIRiG72201.
RefSeqiNP_229658.1. NC_000853.1.
WP_004082413.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36924; AAD36924; TM_1862.
GeneIDi897803.
KEGGitma:TM1862.
PATRICi23938713. VBITheMar51294_1883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36924.1.
PIRiG72201.
RefSeqiNP_229658.1. NC_000853.1.
WP_004082413.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QGQX-ray2.00A/B/C/D/E/F/G/H135-430[»]
4JC0X-ray3.30A/B1-430[»]
ProteinModelPortaliQ9X2H6.
SMRiQ9X2H6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36924; AAD36924; TM_1862.
GeneIDi897803.
KEGGitma:TM1862.
PATRICi23938713. VBITheMar51294_1883.

Phylogenomic databases

eggNOGiENOG4105CBM. Bacteria.
COG0621. LUCA.
InParanoidiQ9X2H6.
KOiK14441.
OMAiHYAYPTG.

Enzyme and pathway databases

BRENDAi2.8.4.4. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X2H6.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIMO_THEMA
AccessioniPrimary (citable) accession number: Q9X2H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.