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Protein

NAD kinase

Gene

NADK

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP (By similarity).By similarity

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Proton acceptorUniRule annotation1
Binding sitei130NADUniRule annotation1
Binding sitei149NADUniRule annotation1
Binding sitei184NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 52NADUniRule annotation2
Nucleotide bindingi119 – 120NADUniRule annotation2
Nucleotide bindingi160 – 165NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.23. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:NADK
Ordered Locus Names:TM_1733
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001206801 – 258NAD kinaseAdd BLAST258

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1733.

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi9 – 11Combined sources3
Helixi12 – 22Combined sources11
Turni23 – 25Combined sources3
Beta strandi26 – 36Combined sources11
Beta strandi43 – 49Combined sources7
Helixi51 – 58Combined sources8
Beta strandi66 – 74Combined sources9
Helixi83 – 85Combined sources3
Helixi86 – 94Combined sources9
Beta strandi99 – 110Combined sources12
Beta strandi113 – 124Combined sources12
Beta strandi132 – 138Combined sources7
Beta strandi144 – 154Combined sources11
Helixi157 – 160Combined sources4
Turni161 – 167Combined sources7
Beta strandi177 – 184Combined sources8
Beta strandi193 – 196Combined sources4
Beta strandi201 – 213Combined sources13
Beta strandi216 – 237Combined sources22
Helixi243 – 251Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YT5X-ray2.30A/B/C/D1-258[»]
ProteinModelPortaliQ9X255.
SMRiQ9X255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X255.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
InParanoidiQ9X255.
KOiK00858.
OMAiMEVAFIN.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAILYREE REKEGEFLKE KISKEHEVIE FGEANAPGRV TADLIVVVGG
60 70 80 90 100
DGTVLKAAKK AADGTPMVGF KAGRLGFLTS YTLDEIDRFL EDLRNWNFRE
110 120 130 140 150
ETRWFIQIES ELGNHLALND VTLERDLSGK MVEIEVEVEH HSSMWFFADG
160 170 180 190 200
VVISTPTGST AYSLSIGGPI IFPECEVLEI SPIAPQFFLT RSVVIPSNFK
210 220 230 240 250
VVVESQRDIN MLVDGVLTGK TKRIEVKKSR RYVRILRPPE YDYVTVIRDK

LGYGRRIE
Length:258
Mass (Da):29,242
Last modified:November 1, 1999 - v1
Checksum:i45EBBCA0B6FD3EAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36798.1.
PIRiD72217.
RefSeqiNP_229531.1. NC_000853.1.
WP_004082250.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36798; AAD36798; TM_1733.
GeneIDi897872.
KEGGitma:TM1733.
PATRICi23938442. VBITheMar51294_1751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36798.1.
PIRiD72217.
RefSeqiNP_229531.1. NC_000853.1.
WP_004082250.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YT5X-ray2.30A/B/C/D1-258[»]
ProteinModelPortaliQ9X255.
SMRiQ9X255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1733.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36798; AAD36798; TM_1733.
GeneIDi897872.
KEGGitma:TM1733.
PATRICi23938442. VBITheMar51294_1751.

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
InParanoidiQ9X255.
KOiK00858.
OMAiMEVAFIN.

Enzyme and pathway databases

BRENDAi2.7.1.23. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X255.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_THEMA
AccessioniPrimary (citable) accession number: Q9X255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.