Q9X248 (FABG_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG
EC=1.1.1.100

Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase

Gene names

Name:	fabG
Ordered Locus Names:	TM_1724

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis By similarity.
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	3-oxoacyl-[acyl-carrier-protein] reductase FabG		PRO_0000054693
Regions
	Nucleotide binding	62 – 63	2	NADP By similarity
	Nucleotide binding	154 – 158	5	NADP By similarity
Sites
	Active site	154	1	Proton acceptor By similarity
	Binding site	89	1	NADP; via carbonyl oxygen By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	187	1	NADP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X248 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8C08904D28099142	FASTA	246	26,402
	10 20 30 40 50 60 MRLEGKVCLI TGAASGIGKA TTLLFAQEGA TVIAGDISKE NLDSLVKEAE GLPGKVDPYV 70 80 90 100 110 120 LNVTDRDQIK EVVEKVVQKY GRIDVLVNNA GITRDALLVR MKEEDWDAVI NVNLKGVFNV 130 140 150 160 170 180 TQMVVPYMIK QRNGSIVNVS SVVGIYGNPG QTNYAASKAG VIGMTKTWAK ELAGRNIRVN 190 200 210 220 230 240 AVAPGFIETP MTEKLPEKAR ETALSRIPLG RFGKPEEVAQ VILFLASDES SYVTGQVIGI DGGLVI

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD36790.1.
PIR	H72219.
RefSeq	NP_229523.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9X248.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897230.
GenomeReviews	Gene locus TM_1724 in contig AE000512_GR.
KEGG	tma:TM1724.
NMPDR	fig|243274.1.peg.1707.
PATRIC	23938424. VBITheMar51294_1742.
TIGR	TM_1724.
Phylogenomic databases
HOGENOM	HBG750976.
OMA	CIITGAA.
PhylomeDB	Q9X248.
ProtClustDB	CLSK875935.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_1724-MONOMER.
Family and domain databases
InterPro	IPR011284. 3oxo_ACP_reduc. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00059.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG_THEMA

Accession

Primary (citable) accession number: Q9X248

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program