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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei264ATPUniRule annotation1
Binding sitei286ATP; via amide nitrogenUniRule annotation1
Binding sitei295ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 181ATPUniRule annotation2
Nucleotide bindingi205 – 206ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BRENDAi2.8.1.B3. 6331.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:TM_1694
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548801 – 388Probable tRNA sulfurtransferaseAdd BLAST388

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1694.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi12 – 14Combined sources3
Helixi21 – 36Combined sources16
Beta strandi41 – 43Combined sources3
Beta strandi46 – 50Combined sources5
Helixi58 – 61Combined sources4
Beta strandi69 – 76Combined sources8
Helixi80 – 95Combined sources16
Beta strandi102 – 111Combined sources10
Beta strandi114 – 116Combined sources3
Helixi118 – 132Combined sources15
Beta strandi140 – 142Combined sources3
Beta strandi144 – 152Combined sources9
Beta strandi155 – 162Combined sources8
Beta strandi176 – 180Combined sources5
Beta strandi183 – 185Combined sources3
Helixi186 – 196Combined sources11
Beta strandi200 – 207Combined sources8
Turni209 – 211Combined sources3
Helixi216 – 227Combined sources12
Helixi228 – 230Combined sources3
Turni231 – 233Combined sources3
Beta strandi236 – 242Combined sources7
Helixi244 – 253Combined sources10
Helixi256 – 258Combined sources3
Helixi259 – 278Combined sources20
Beta strandi282 – 284Combined sources3
Beta strandi289 – 292Combined sources4
Helixi293 – 295Combined sources3
Helixi297 – 304Combined sources8
Turni314 – 317Combined sources4
Helixi320 – 329Combined sources10
Turni333 – 335Combined sources3
Beta strandi346 – 348Combined sources3
Helixi358 – 364Combined sources7
Helixi365 – 367Combined sources3
Helixi371 – 380Combined sources10
Beta strandi383 – 387Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KR6X-ray2.85A/B1-388[»]
4KR7X-ray3.42A/B1-388[»]
4KR9X-ray3.50A/B1-388[»]
ProteinModelPortaliQ9X220.
SMRiQ9X220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 162THUMPUniRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
InParanoidiQ9X220.
KOiK03151.
OMAiPDEDCCT.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKELRVYIVR YSEIGLKGKN RKDFEEALRR NIERVTGMKV KRQWGRFLIP
60 70 80 90 100
IDENVTLDDK LKKIFGIQNF SKGFLVSHDF EEVKKYSLIA VKEKLEKGNY
110 120 130 140 150
RTFKVQAKKA YKEYKKGVYE INSELGALIL KNFKELSVDV RNPDFVLGVE
160 170 180 190 200
VRPEGVLIFT DRVECYGGLP VGTGGKAVLL LSGGIDSPVA GWYALKRGVL
210 220 230 240 250
IESVTFVSPP FTSEGAVEKV RDILRVLREF SGGHPLRLHI VNLTKLQLEV
260 270 280 290 300
KKRVPDKYSL IMYRRSMFRI AEKIAEETGA VAFYTGENIG QVASQTLENL
310 320 330 340 350
WSIESVTTRP VIRPLSGFDK TEIVEKAKEI GTYEISIKPY QDSCVFFAPK
360 370 380
NPATRSHPSI LEKLEQQVPD LPVLEEEAFT SRKVEVIE
Length:388
Mass (Da):44,126
Last modified:November 1, 1999 - v1
Checksum:iD8BE03E3EFEEB856
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36761.1.
PIRiH72223.
RefSeqiNP_229494.1. NC_000853.1.
WP_010865383.1. NC_000853.1.

Genome annotation databases

EnsemblBacteriaiAAD36761; AAD36761; TM_1694.
GeneIDi897892.
KEGGitma:TM1694.
tmi:THEMA_05770.
PATRICi23938362. VBITheMar51294_1711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36761.1.
PIRiH72223.
RefSeqiNP_229494.1. NC_000853.1.
WP_010865383.1. NC_000853.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KR6X-ray2.85A/B1-388[»]
4KR7X-ray3.42A/B1-388[»]
4KR9X-ray3.50A/B1-388[»]
ProteinModelPortaliQ9X220.
SMRiQ9X220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36761; AAD36761; TM_1694.
GeneIDi897892.
KEGGitma:TM1694.
tmi:THEMA_05770.
PATRICi23938362. VBITheMar51294_1711.

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
InParanoidiQ9X220.
KOiK03151.
OMAiPDEDCCT.

Enzyme and pathway databases

UniPathwayiUPA00060.
BRENDAi2.8.1.B3. 6331.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHII_THEMA
AccessioniPrimary (citable) accession number: Q9X220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.