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Q9X220

- THII_THEMA

UniProt

Q9X220 - THII_THEMA

Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei264 – 2641ATPUniRule annotation
    Binding sitei286 – 2861ATP; via amide nitrogenUniRule annotation
    Binding sitei295 – 2951ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi180 – 1812ATPUniRule annotation
    Nucleotide bindingi205 – 2062ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-1742-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:TM_1694
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Probable tRNA sulfurtransferasePRO_0000154880Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243274.TM1694.

    Structurei

    Secondary structure

    1
    388
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 107
    Helixi12 – 143
    Helixi21 – 3616
    Beta strandi41 – 433
    Beta strandi46 – 505
    Helixi58 – 614
    Beta strandi69 – 768
    Helixi80 – 9516
    Beta strandi102 – 11110
    Beta strandi114 – 1163
    Helixi118 – 13215
    Beta strandi140 – 1423
    Beta strandi144 – 1529
    Beta strandi155 – 1628
    Beta strandi176 – 1805
    Beta strandi183 – 1853
    Helixi186 – 19611
    Beta strandi200 – 2078
    Turni209 – 2113
    Helixi216 – 22712
    Helixi228 – 2303
    Turni231 – 2333
    Beta strandi236 – 2427
    Helixi244 – 25310
    Helixi256 – 2583
    Helixi259 – 27820
    Beta strandi282 – 2843
    Beta strandi289 – 2924
    Helixi293 – 2953
    Helixi297 – 3048
    Turni314 – 3174
    Helixi320 – 32910
    Turni333 – 3353
    Beta strandi346 – 3483
    Helixi358 – 3647
    Helixi365 – 3673
    Helixi371 – 38010
    Beta strandi383 – 3875

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4KR6X-ray2.85A/B1-388[»]
    4KR7X-ray3.42A/B1-388[»]
    4KR9X-ray3.50A/B1-388[»]
    ProteinModelPortaliQ9X220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 162108THUMPUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0301.
    KOiK03151.
    OMAiAQMKGPD.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
    PROSITEiPS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X220-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKELRVYIVR YSEIGLKGKN RKDFEEALRR NIERVTGMKV KRQWGRFLIP    50
    IDENVTLDDK LKKIFGIQNF SKGFLVSHDF EEVKKYSLIA VKEKLEKGNY 100
    RTFKVQAKKA YKEYKKGVYE INSELGALIL KNFKELSVDV RNPDFVLGVE 150
    VRPEGVLIFT DRVECYGGLP VGTGGKAVLL LSGGIDSPVA GWYALKRGVL 200
    IESVTFVSPP FTSEGAVEKV RDILRVLREF SGGHPLRLHI VNLTKLQLEV 250
    KKRVPDKYSL IMYRRSMFRI AEKIAEETGA VAFYTGENIG QVASQTLENL 300
    WSIESVTTRP VIRPLSGFDK TEIVEKAKEI GTYEISIKPY QDSCVFFAPK 350
    NPATRSHPSI LEKLEQQVPD LPVLEEEAFT SRKVEVIE 388
    Length:388
    Mass (Da):44,126
    Last modified:November 1, 1999 - v1
    Checksum:iD8BE03E3EFEEB856
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36761.1.
    PIRiH72223.
    RefSeqiNP_229494.1. NC_000853.1.
    WP_010865383.1. NC_023151.1.
    YP_008991213.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36761; AAD36761; TM_1694.
    GeneIDi897892.
    KEGGitma:TM1694.
    tmi:THEMA_05770.
    PATRICi23938362. VBITheMar51294_1711.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36761.1 .
    PIRi H72223.
    RefSeqi NP_229494.1. NC_000853.1.
    WP_010865383.1. NC_023151.1.
    YP_008991213.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4KR6 X-ray 2.85 A/B 1-388 [» ]
    4KR7 X-ray 3.42 A/B 1-388 [» ]
    4KR9 X-ray 3.50 A/B 1-388 [» ]
    ProteinModelPortali Q9X220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1694.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36761 ; AAD36761 ; TM_1694 .
    GeneIDi 897892.
    KEGGi tma:TM1694.
    tmi:THEMA_05770.
    PATRICi 23938362. VBITheMar51294_1711.

    Phylogenomic databases

    eggNOGi COG0301.
    KOi K03151.
    OMAi AQMKGPD.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci TMAR243274:GC6P-1742-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiTHII_THEMA
    AccessioniPrimary (citable) accession number: Q9X220
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3