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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei264 – 2641ATPUniRule annotation
Binding sitei286 – 2861ATP; via amide nitrogenUniRule annotation
Binding sitei295 – 2951ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi180 – 1812ATPUniRule annotation
Nucleotide bindingi205 – 2062ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1742-MONOMER.
BRENDAi2.8.1.B3. 6331.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:TM_1694
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Probable tRNA sulfurtransferasePRO_0000154880Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1694.

Structurei

Secondary structure

1
388
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Helixi12 – 143Combined sources
Helixi21 – 3616Combined sources
Beta strandi41 – 433Combined sources
Beta strandi46 – 505Combined sources
Helixi58 – 614Combined sources
Beta strandi69 – 768Combined sources
Helixi80 – 9516Combined sources
Beta strandi102 – 11110Combined sources
Beta strandi114 – 1163Combined sources
Helixi118 – 13215Combined sources
Beta strandi140 – 1423Combined sources
Beta strandi144 – 1529Combined sources
Beta strandi155 – 1628Combined sources
Beta strandi176 – 1805Combined sources
Beta strandi183 – 1853Combined sources
Helixi186 – 19611Combined sources
Beta strandi200 – 2078Combined sources
Turni209 – 2113Combined sources
Helixi216 – 22712Combined sources
Helixi228 – 2303Combined sources
Turni231 – 2333Combined sources
Beta strandi236 – 2427Combined sources
Helixi244 – 25310Combined sources
Helixi256 – 2583Combined sources
Helixi259 – 27820Combined sources
Beta strandi282 – 2843Combined sources
Beta strandi289 – 2924Combined sources
Helixi293 – 2953Combined sources
Helixi297 – 3048Combined sources
Turni314 – 3174Combined sources
Helixi320 – 32910Combined sources
Turni333 – 3353Combined sources
Beta strandi346 – 3483Combined sources
Helixi358 – 3647Combined sources
Helixi365 – 3673Combined sources
Helixi371 – 38010Combined sources
Beta strandi383 – 3875Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KR6X-ray2.85A/B1-388[»]
4KR7X-ray3.42A/B1-388[»]
4KR9X-ray3.50A/B1-388[»]
ProteinModelPortaliQ9X220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 162108THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0301.
InParanoidiQ9X220.
KOiK03151.
OMAiPDEDCCT.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKELRVYIVR YSEIGLKGKN RKDFEEALRR NIERVTGMKV KRQWGRFLIP
60 70 80 90 100
IDENVTLDDK LKKIFGIQNF SKGFLVSHDF EEVKKYSLIA VKEKLEKGNY
110 120 130 140 150
RTFKVQAKKA YKEYKKGVYE INSELGALIL KNFKELSVDV RNPDFVLGVE
160 170 180 190 200
VRPEGVLIFT DRVECYGGLP VGTGGKAVLL LSGGIDSPVA GWYALKRGVL
210 220 230 240 250
IESVTFVSPP FTSEGAVEKV RDILRVLREF SGGHPLRLHI VNLTKLQLEV
260 270 280 290 300
KKRVPDKYSL IMYRRSMFRI AEKIAEETGA VAFYTGENIG QVASQTLENL
310 320 330 340 350
WSIESVTTRP VIRPLSGFDK TEIVEKAKEI GTYEISIKPY QDSCVFFAPK
360 370 380
NPATRSHPSI LEKLEQQVPD LPVLEEEAFT SRKVEVIE
Length:388
Mass (Da):44,126
Last modified:November 1, 1999 - v1
Checksum:iD8BE03E3EFEEB856
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36761.1.
PIRiH72223.
RefSeqiNP_229494.1. NC_000853.1.
WP_010865383.1. NC_023151.1.
YP_008991213.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD36761; AAD36761; TM_1694.
GeneIDi897892.
KEGGitma:TM1694.
tmi:THEMA_05770.
PATRICi23938362. VBITheMar51294_1711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36761.1.
PIRiH72223.
RefSeqiNP_229494.1. NC_000853.1.
WP_010865383.1. NC_023151.1.
YP_008991213.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KR6X-ray2.85A/B1-388[»]
4KR7X-ray3.42A/B1-388[»]
4KR9X-ray3.50A/B1-388[»]
ProteinModelPortaliQ9X220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36761; AAD36761; TM_1694.
GeneIDi897892.
KEGGitma:TM1694.
tmi:THEMA_05770.
PATRICi23938362. VBITheMar51294_1711.

Phylogenomic databases

eggNOGiCOG0301.
InParanoidiQ9X220.
KOiK03151.
OMAiPDEDCCT.
OrthoDBiEOG6TBHGR.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciTMAR243274:GC6P-1742-MONOMER.
BRENDAi2.8.1.B3. 6331.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiTHII_THEMA
AccessioniPrimary (citable) accession number: Q9X220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.