Q9X220 (THII_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA sulfurtransferase
EC=2.8.1.4

Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase

Gene names

Name:	thiI
Ordered Locus Names:	TM_1694

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021
Catalytic activity	L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021 [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ThiI family. Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA thio-modification Inferred from electronic annotation. Source: HAMAP thiamine biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Probable tRNA sulfurtransferase HAMAP-Rule MF_00021		PRO_0000154880
Regions
	Domain	55 – 162	108	THUMP
	Nucleotide binding	180 – 181	2	ATP By similarity
	Nucleotide binding	205 – 206	2	ATP By similarity
Sites
	Binding site	264	1	ATP By similarity
	Binding site	286	1	ATP; via amide nitrogen By similarity
	Binding site	295	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X220 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D8BE03E3EFEEB856	FASTA	388	44,126
	10 20 30 40 50 60 MKELRVYIVR YSEIGLKGKN RKDFEEALRR NIERVTGMKV KRQWGRFLIP IDENVTLDDK 70 80 90 100 110 120 LKKIFGIQNF SKGFLVSHDF EEVKKYSLIA VKEKLEKGNY RTFKVQAKKA YKEYKKGVYE 130 140 150 160 170 180 INSELGALIL KNFKELSVDV RNPDFVLGVE VRPEGVLIFT DRVECYGGLP VGTGGKAVLL 190 200 210 220 230 240 LSGGIDSPVA GWYALKRGVL IESVTFVSPP FTSEGAVEKV RDILRVLREF SGGHPLRLHI 250 260 270 280 290 300 VNLTKLQLEV KKRVPDKYSL IMYRRSMFRI AEKIAEETGA VAFYTGENIG QVASQTLENL 310 320 330 340 350 360 WSIESVTTRP VIRPLSGFDK TEIVEKAKEI GTYEISIKPY QDSCVFFAPK NPATRSHPSI 370 380 LEKLEQQVPD LPVLEEEAFT SRKVEVIE

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD36761.1.
PIR	H72223.
RefSeq	NP_229494.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9X220.
ModBase	Search...
Protein-protein interaction databases
STRING	243274.TM1694.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD36761; AAD36761; TM_1694.
GeneID	897892.
KEGG	tma:TM1694.
PATRIC	23938362. VBITheMar51294_1711.
Phylogenomic databases
eggNOG	COG0301.
KO	K03151.
OMA	KLTPYVG.
ProtClustDB	PRK01565.
Enzyme and pathway databases
UniPathway	UPA00060.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00021. ThiI.
InterPro	IPR014729. Rossmann-like_a/b/a_fold. IPR003720. ThiI. IPR020536. ThiI_C_dom. IPR004114. THUMP. [Graphical view]
Pfam	PF02568. ThiI. 1 hit. PF02926. THUMP. 1 hit. [Graphical view]
SMART	SM00981. THUMP. 1 hit. [Graphical view]
TIGRFAMs	TIGR00342. TIGR00342. 1 hit.
PROSITE	PS51165. THUMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THII_THEMA

Accession

Primary (citable) accession number: Q9X220

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program