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Q9X220 (THII_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA sulfurtransferase

EC=2.8.1.4
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene names
Name:thiI
Ordered Locus Names:TM_1694
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021

Catalytic activity

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021

[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00021.

Sequence similarities

Belongs to the ThiI family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processThiamine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA thio-modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thiamine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thiamine diphosphate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

sulfurtransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388Probable tRNA sulfurtransferase HAMAP-Rule MF_00021
PRO_0000154880

Regions

Domain55 – 162108THUMP
Nucleotide binding180 – 1812ATP By similarity
Nucleotide binding205 – 2062ATP By similarity

Sites

Binding site2641ATP By similarity
Binding site2861ATP; via amide nitrogen By similarity
Binding site2951ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9X220 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: D8BE03E3EFEEB856

FASTA38844,126
        10         20         30         40         50         60 
MKELRVYIVR YSEIGLKGKN RKDFEEALRR NIERVTGMKV KRQWGRFLIP IDENVTLDDK 

        70         80         90        100        110        120 
LKKIFGIQNF SKGFLVSHDF EEVKKYSLIA VKEKLEKGNY RTFKVQAKKA YKEYKKGVYE 

       130        140        150        160        170        180 
INSELGALIL KNFKELSVDV RNPDFVLGVE VRPEGVLIFT DRVECYGGLP VGTGGKAVLL 

       190        200        210        220        230        240 
LSGGIDSPVA GWYALKRGVL IESVTFVSPP FTSEGAVEKV RDILRVLREF SGGHPLRLHI 

       250        260        270        280        290        300 
VNLTKLQLEV KKRVPDKYSL IMYRRSMFRI AEKIAEETGA VAFYTGENIG QVASQTLENL 

       310        320        330        340        350        360 
WSIESVTTRP VIRPLSGFDK TEIVEKAKEI GTYEISIKPY QDSCVFFAPK NPATRSHPSI 

       370        380 
LEKLEQQVPD LPVLEEEAFT SRKVEVIE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD36761.1.
PIRH72223.
RefSeqNP_229494.1. NC_000853.1.
YP_008991213.1. NC_023151.1.

3D structure databases

ProteinModelPortalQ9X220.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM1694.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36761; AAD36761; TM_1694.
GeneID18093004.
897892.
KEGGtma:TM1694.
PATRIC23938362. VBITheMar51294_1711.

Phylogenomic databases

eggNOGCOG0301.
KOK03151.
OMAKLTPYVG.
OrthoDBEOG6TBHGR.
ProtClustDBPRK01565.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-1742-MONOMER.
UniPathwayUPA00060.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00021. ThiI.
InterProIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_C_dom.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsTIGR00342. TIGR00342. 1 hit.
PROSITEPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHII_THEMA
AccessionPrimary (citable) accession number: Q9X220
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: March 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways