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Q9X220

- THII_THEMA

UniProt

Q9X220 - THII_THEMA

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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei264 – 2641ATPUniRule annotation
Binding sitei286 – 2861ATP; via amide nitrogenUniRule annotation
Binding sitei295 – 2951ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi180 – 1812ATPUniRule annotation
Nucleotide bindingi205 – 2062ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA adenylyltransferase activity Source: InterPro
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. tRNA thio-modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1742-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:TM_1694
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Probable tRNA sulfurtransferasePRO_0000154880Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1694.

Structurei

Secondary structure

1
388
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107
Helixi12 – 143
Helixi21 – 3616
Beta strandi41 – 433
Beta strandi46 – 505
Helixi58 – 614
Beta strandi69 – 768
Helixi80 – 9516
Beta strandi102 – 11110
Beta strandi114 – 1163
Helixi118 – 13215
Beta strandi140 – 1423
Beta strandi144 – 1529
Beta strandi155 – 1628
Beta strandi176 – 1805
Beta strandi183 – 1853
Helixi186 – 19611
Beta strandi200 – 2078
Turni209 – 2113
Helixi216 – 22712
Helixi228 – 2303
Turni231 – 2333
Beta strandi236 – 2427
Helixi244 – 25310
Helixi256 – 2583
Helixi259 – 27820
Beta strandi282 – 2843
Beta strandi289 – 2924
Helixi293 – 2953
Helixi297 – 3048
Turni314 – 3174
Helixi320 – 32910
Turni333 – 3353
Beta strandi346 – 3483
Helixi358 – 3647
Helixi365 – 3673
Helixi371 – 38010
Beta strandi383 – 3875

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KR6X-ray2.85A/B1-388[»]
4KR7X-ray3.42A/B1-388[»]
4KR9X-ray3.50A/B1-388[»]
ProteinModelPortaliQ9X220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 162108THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0301.
InParanoidiQ9X220.
KOiK03151.
OMAiAQMKGPD.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X220-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKELRVYIVR YSEIGLKGKN RKDFEEALRR NIERVTGMKV KRQWGRFLIP
60 70 80 90 100
IDENVTLDDK LKKIFGIQNF SKGFLVSHDF EEVKKYSLIA VKEKLEKGNY
110 120 130 140 150
RTFKVQAKKA YKEYKKGVYE INSELGALIL KNFKELSVDV RNPDFVLGVE
160 170 180 190 200
VRPEGVLIFT DRVECYGGLP VGTGGKAVLL LSGGIDSPVA GWYALKRGVL
210 220 230 240 250
IESVTFVSPP FTSEGAVEKV RDILRVLREF SGGHPLRLHI VNLTKLQLEV
260 270 280 290 300
KKRVPDKYSL IMYRRSMFRI AEKIAEETGA VAFYTGENIG QVASQTLENL
310 320 330 340 350
WSIESVTTRP VIRPLSGFDK TEIVEKAKEI GTYEISIKPY QDSCVFFAPK
360 370 380
NPATRSHPSI LEKLEQQVPD LPVLEEEAFT SRKVEVIE
Length:388
Mass (Da):44,126
Last modified:November 1, 1999 - v1
Checksum:iD8BE03E3EFEEB856
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36761.1.
PIRiH72223.
RefSeqiNP_229494.1. NC_000853.1.
YP_008991213.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD36761; AAD36761; TM_1694.
GeneIDi18093004.
897892.
KEGGitma:TM1694.
tmi:THEMA_05770.
PATRICi23938362. VBITheMar51294_1711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36761.1 .
PIRi H72223.
RefSeqi NP_229494.1. NC_000853.1.
YP_008991213.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4KR6 X-ray 2.85 A/B 1-388 [» ]
4KR7 X-ray 3.42 A/B 1-388 [» ]
4KR9 X-ray 3.50 A/B 1-388 [» ]
ProteinModelPortali Q9X220.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM1694.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36761 ; AAD36761 ; TM_1694 .
GeneIDi 18093004.
897892.
KEGGi tma:TM1694.
tmi:THEMA_05770.
PATRICi 23938362. VBITheMar51294_1711.

Phylogenomic databases

eggNOGi COG0301.
InParanoidi Q9X220.
KOi K03151.
OMAi AQMKGPD.
OrthoDBi EOG6TBHGR.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci TMAR243274:GC6P-1742-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
PROSITEi PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiTHII_THEMA
AccessioniPrimary (citable) accession number: Q9X220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3