Q9X1Z1 (ECFA1_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Energy-coupling factor transporter ATP-binding protein EcfA1
Short name=ECF transporter A component EcfA1
EC=3.6.3.-

Gene names

Name:	ecfA1
Synonyms:	cbiO, ecfA'
Ordered Locus Names:	TM_1663

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates Probable. Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex. Ref.2
Subunit structure	Forms a heterodimer with EcfA2. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU. Ref.2
Subcellular location	Cell inner membrane; Peripheral membrane protein Probable Ref.2.
Miscellaneous	Structure 4HLU is probably in the open state.
Sequence similarities	Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	ATP binding Inferred from direct assay Ref.2. Source: UniProtKB ATPase activity Inferred from electronic annotation. Source: InterPro riboflavin transporter activity Inferred from genetic interaction Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 259

259

Energy-coupling factor transporter ATP-binding protein EcfA1

PRO_0000092116

Regions

Domain

3 – 230

228

ABC transporter

Nucleotide binding

38 – 43

6

ATP

Sites

Active site

157

1

Proton acceptor Potential

Secondary structure

1

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259

Helix Strand Turn

Details...

Beta strand

9 – 18

10

Beta strand

30 – 34

5

Helix

41 – 49

9

Helix

67 – 73

7

Beta strand

74 – 77

4

Beta strand

79 – 81

3

Turn

82 – 84

3

Helix

90 – 95

6

Beta strand

98 – 101

4

Helix

108 – 118

11

Helix

129 – 131

3

Helix

134 – 146

13

Beta strand

151 – 157

7

Turn

158 – 161

4

Helix

164 – 178

15

Turn

179 – 181

3

Beta strand

183 – 187

5

Helix

191 – 193

3

Beta strand

199 – 204

6

Beta strand

207 – 212

6

Helix

214 – 220

7

Beta strand

223 – 225

3

Helix

229 – 237

9

Sequences

	Sequence		Length	Mass (Da)
	Q9X1Z1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 80B492FFF04A2C6A	FASTA	259	29,376
	10 20 30 40 50 60 MKITLNSVSF RYNGDYVLKD VNAEFETGKI YVVVGKNGSG KTTLLKILAG LLEAEGEIFL 70 80 90 100 110 120 DGSPADPFLL RKNVGYVFQN PSSQIIGATV EEDVAFSLEI MGLDESEMRK RIKKVLELVG 130 140 150 160 170 180 LSGLEKEDPL NLSGGQKQRL AIASMLARDT RFLALDEPVS MLDPPSQREI FQVLESLKNE 190 200 210 220 230 240 GKGIILVTHE LEYLDDMDFI LHISNGTIDF CGSWEEFVER EFDDVEIPFK WKLWKKCGKI 250 NLWEDRYENS GNQRRRDTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Assembly and mechanism of a group II ECF transporter." Karpowich N.K., Wang D.N. Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, EXPRESSION IN E.COLI, ATP-BINDING, X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 5-259 IN COMPLEX WITH ADP. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000512 Genomic DNA. Translation: AAD36730.1.

PIR

E72224.

RefSeq

NP_229463.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HLU	X-ray	2.70	C/D	5-259	[»]

ProteinModelPortal

Q9X1Z1.

ModBase

Search...

Protein-protein interaction databases

STRING

243274.TM1663.

Protocols and materials databases

DNASU

897908.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD36730; AAD36730; TM_1663.

GeneID

897908.

KEGG

tma:TM1663.

PATRIC

23938300. VBITheMar51294_1682.

Phylogenomic databases

eggNOG

COG1122.

KO

K16786.

OMA

DESEMRK.

ProtClustDB

CLSK796080.

Family and domain databases

InterPro

IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015856. ABC_transpr_CbiO.
[Graphical view]

Pfam

PF00005. ABC_tran. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

PROSITE

PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51246. CBIO. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ECFA1_THEMA

Accession

Primary (citable) accession number: Q9X1Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program