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Protein

Energy-coupling factor transporter ATP-binding protein EcfA1

Gene

ecfA1

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (Probable). Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.UniRule annotationCurated1 Publication

Miscellaneous

Structure 4HLU is probably in the open state.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei157Proton acceptorSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 43ATP6

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB
  • riboflavin transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • riboflavin transport Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.25.5 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Energy-coupling factor transporter ATP-binding protein EcfA1UniRule annotation (EC:3.6.3.-UniRule annotation)
Short name:
ECF transporter A component EcfA1UniRule annotation
Gene namesi
Name:ecfA1UniRule annotation
Synonyms:cbiO, ecfA'
Ordered Locus Names:TM_1663
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000921161 – 259Energy-coupling factor transporter ATP-binding protein EcfA1Add BLAST259

Interactioni

Subunit structurei

Forms a heterodimer with EcfA2. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ecfA2Q9WY652EBI-16160756,EBI-16160779

Protein-protein interaction databases

DIPiDIP-61611N
IntActiQ9X1Z1, 1 interactor
STRINGi243274.TM1663

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi9 – 18Combined sources10
Beta strandi30 – 34Combined sources5
Helixi41 – 49Combined sources9
Beta strandi55 – 60Combined sources6
Helixi67 – 73Combined sources7
Beta strandi74 – 77Combined sources4
Beta strandi79 – 81Combined sources3
Turni82 – 84Combined sources3
Beta strandi87 – 89Combined sources3
Helixi90 – 95Combined sources6
Beta strandi98 – 101Combined sources4
Helixi108 – 118Combined sources11
Helixi129 – 131Combined sources3
Helixi134 – 146Combined sources13
Beta strandi151 – 157Combined sources7
Turni158 – 161Combined sources4
Helixi164 – 178Combined sources15
Turni179 – 181Combined sources3
Beta strandi183 – 187Combined sources5
Helixi191 – 193Combined sources3
Beta strandi199 – 204Combined sources6
Beta strandi207 – 212Combined sources6
Helixi214 – 220Combined sources7
Beta strandi223 – 225Combined sources3
Helixi229 – 237Combined sources9
Helixi242 – 246Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HLUX-ray2.70C/D2-259[»]
4ZIRX-ray3.00B2-259[»]
ProteinModelPortaliQ9X1Z1
SMRiQ9X1Z1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 230ABC transporterUniRule annotationAdd BLAST228

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JJB Bacteria
COG1122 LUCA
InParanoidiQ9X1Z1
KOiK16786
OMAiDHQLVMP

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR015856 ABC_transpr_CbiO/EcfA_su
IPR030947 EcfA_1
IPR027417 P-loop_NTPase
PANTHERiPTHR43553:SF4 PTHR43553:SF4, 1 hit
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51246 CBIO, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X1Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITLNSVSF RYNGDYVLKD VNAEFETGKI YVVVGKNGSG KTTLLKILAG
60 70 80 90 100
LLEAEGEIFL DGSPADPFLL RKNVGYVFQN PSSQIIGATV EEDVAFSLEI
110 120 130 140 150
MGLDESEMRK RIKKVLELVG LSGLEKEDPL NLSGGQKQRL AIASMLARDT
160 170 180 190 200
RFLALDEPVS MLDPPSQREI FQVLESLKNE GKGIILVTHE LEYLDDMDFI
210 220 230 240 250
LHISNGTIDF CGSWEEFVER EFDDVEIPFK WKLWKKCGKI NLWEDRYENS

GNQRRRDTV
Length:259
Mass (Da):29,376
Last modified:November 1, 1999 - v1
Checksum:i80B492FFF04A2C6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36730.1
PIRiE72224
RefSeqiNP_229463.1, NC_000853.1
WP_004082181.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD36730; AAD36730; TM_1663
GeneIDi897908
KEGGitma:TM1663

Similar proteinsi

Entry informationi

Entry nameiECFA1_THEMA
AccessioniPrimary (citable) accession number: Q9X1Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health