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Q9X1Z1

- ECFA1_THEMA

UniProt

Q9X1Z1 - ECFA1_THEMA

Protein

Energy-coupling factor transporter ATP-binding protein EcfA1

Gene

ecfA1

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates Probable. Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.1 PublicationCuratedUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei157 – 1571Proton acceptorSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 436ATP

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. ATP binding Source: UniProtKB
    3. riboflavin transporter activity Source: UniProtKB

    GO - Biological processi

    1. riboflavin transport Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    TCDBi3.A.1.25.5. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Energy-coupling factor transporter ATP-binding protein EcfA1UniRule annotation (EC:3.6.3.-UniRule annotation)
    Short name:
    ECF transporter A component EcfA1UniRule annotation
    Gene namesi
    Name:ecfA1UniRule annotation
    Synonyms:cbiO, ecfA'
    Ordered Locus Names:TM_1663
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
    2. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 259259Energy-coupling factor transporter ATP-binding protein EcfA1PRO_0000092116Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a heterodimer with EcfA2. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU.1 Publication

    Protein-protein interaction databases

    STRINGi243274.TM1663.

    Structurei

    Secondary structure

    1
    259
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 1810
    Beta strandi30 – 345
    Helixi41 – 499
    Helixi67 – 737
    Beta strandi74 – 774
    Beta strandi79 – 813
    Turni82 – 843
    Helixi90 – 956
    Beta strandi98 – 1014
    Helixi108 – 11811
    Helixi129 – 1313
    Helixi134 – 14613
    Beta strandi151 – 1577
    Turni158 – 1614
    Helixi164 – 17815
    Turni179 – 1813
    Beta strandi183 – 1875
    Helixi191 – 1933
    Beta strandi199 – 2046
    Beta strandi207 – 2126
    Helixi214 – 2207
    Beta strandi223 – 2253
    Helixi229 – 2379

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HLUX-ray2.70C/D2-259[»]
    ProteinModelPortaliQ9X1Z1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 230228ABC transporterUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.UniRule annotation
    Contains 1 ABC transporter domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1122.
    KOiK16786.
    OMAiDESEMRK.
    OrthoDBiEOG6T7N3V.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR015856. ABC_transpr_CbiO/EcfA_su.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51246. CBIO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X1Z1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKITLNSVSF RYNGDYVLKD VNAEFETGKI YVVVGKNGSG KTTLLKILAG    50
    LLEAEGEIFL DGSPADPFLL RKNVGYVFQN PSSQIIGATV EEDVAFSLEI 100
    MGLDESEMRK RIKKVLELVG LSGLEKEDPL NLSGGQKQRL AIASMLARDT 150
    RFLALDEPVS MLDPPSQREI FQVLESLKNE GKGIILVTHE LEYLDDMDFI 200
    LHISNGTIDF CGSWEEFVER EFDDVEIPFK WKLWKKCGKI NLWEDRYENS 250
    GNQRRRDTV 259
    Length:259
    Mass (Da):29,376
    Last modified:November 1, 1999 - v1
    Checksum:i80B492FFF04A2C6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36730.1.
    PIRiE72224.
    RefSeqiNP_229463.1. NC_000853.1.
    WP_004082181.1. NC_023151.1.
    YP_007978021.1. NC_021214.1.
    YP_008991243.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36730; AAD36730; TM_1663.
    GeneIDi897908.
    KEGGitma:TM1663.
    tmi:THEMA_05930.
    tmm:Tmari_1672.
    PATRICi23938300. VBITheMar51294_1682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36730.1 .
    PIRi E72224.
    RefSeqi NP_229463.1. NC_000853.1.
    WP_004082181.1. NC_023151.1.
    YP_007978021.1. NC_021214.1.
    YP_008991243.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HLU X-ray 2.70 C/D 2-259 [» ]
    ProteinModelPortali Q9X1Z1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1663.

    Protein family/group databases

    TCDBi 3.A.1.25.5. the atp-binding cassette (abc) superfamily.

    Protocols and materials databases

    DNASUi 897908.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36730 ; AAD36730 ; TM_1663 .
    GeneIDi 897908.
    KEGGi tma:TM1663.
    tmi:THEMA_05930.
    tmm:Tmari_1672.
    PATRICi 23938300. VBITheMar51294_1682.

    Phylogenomic databases

    eggNOGi COG1122.
    KOi K16786.
    OMAi DESEMRK.
    OrthoDBi EOG6T7N3V.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR015856. ABC_transpr_CbiO/EcfA_su.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51246. CBIO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Assembly and mechanism of a group II ECF transporter."
      Karpowich N.K., Wang D.N.
      Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, EXPRESSION IN E.COLI, ATP-BINDING, X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 5-259 IN COMPLEX WITH ADP.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiECFA1_THEMA
    AccessioniPrimary (citable) accession number: Q9X1Z1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Structure 4HLU is probably in the open state.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3