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Q9X1Z1 (ECFA1_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Energy-coupling factor transporter ATP-binding protein EcfA1

Short name=ECF transporter A component EcfA1
EC=3.6.3.-
Gene names
Name:ecfA1
Synonyms:cbiO, ecfA'
Ordered Locus Names:TM_1663
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates Probable. Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex. Ref.2

Subunit structure

Forms a heterodimer with EcfA2. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU. Ref.2

Subcellular location

Cell inner membrane; Peripheral membrane protein Probable Ref.2.

Miscellaneous

Structure 4HLU is probably in the open state.

Sequence similarities

Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.

Contains 1 ABC transporter domain.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processriboflavin transport

Inferred from genetic interaction Ref.2. Source: UniProtKB

   Cellular_componentplasma membrane

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functionATP binding

Inferred from direct assay Ref.2. Source: UniProtKB

ATPase activity

Inferred from electronic annotation. Source: InterPro

riboflavin transporter activity

Inferred from genetic interaction Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259Energy-coupling factor transporter ATP-binding protein EcfA1
PRO_0000092116

Regions

Domain3 – 230228ABC transporter
Nucleotide binding38 – 436ATP

Sites

Active site1571Proton acceptor Potential

Secondary structure

........................................... 259
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9X1Z1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 80B492FFF04A2C6A

FASTA25929,376
        10         20         30         40         50         60 
MKITLNSVSF RYNGDYVLKD VNAEFETGKI YVVVGKNGSG KTTLLKILAG LLEAEGEIFL 

        70         80         90        100        110        120 
DGSPADPFLL RKNVGYVFQN PSSQIIGATV EEDVAFSLEI MGLDESEMRK RIKKVLELVG 

       130        140        150        160        170        180 
LSGLEKEDPL NLSGGQKQRL AIASMLARDT RFLALDEPVS MLDPPSQREI FQVLESLKNE 

       190        200        210        220        230        240 
GKGIILVTHE LEYLDDMDFI LHISNGTIDF CGSWEEFVER EFDDVEIPFK WKLWKKCGKI 

       250 
NLWEDRYENS GNQRRRDTV 

« Hide

References

« Hide 'large scale' references
[1]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Assembly and mechanism of a group II ECF transporter."
Karpowich N.K., Wang D.N.
Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, EXPRESSION IN E.COLI, ATP-BINDING, X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 5-259 IN COMPLEX WITH ADP.
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD36730.1.
PIRE72224.
RefSeqNP_229463.1. NC_000853.1.
YP_007978021.1. NC_021214.1.
YP_008991243.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HLUX-ray2.70C/D5-259[»]
ProteinModelPortalQ9X1Z1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM1663.

Protein family/group databases

TCDB3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

DNASU897908.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36730; AAD36730; TM_1663.
GeneID15495274.
18093036.
897908.
KEGGtma:TM1663.
tmm:Tmari_1672.
PATRIC23938300. VBITheMar51294_1682.

Phylogenomic databases

eggNOGCOG1122.
KOK16786.
OMADESEMRK.
OrthoDBEOG6T7N3V.
ProtClustDBCLSK796080.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015856. ABC_transpr_CbiO.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51246. CBIO. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameECFA1_THEMA
AccessionPrimary (citable) accession number: Q9X1Z1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references