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Q9X1Z1

- ECFA1_THEMA

UniProt

Q9X1Z1 - ECFA1_THEMA

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Protein
Energy-coupling factor transporter ATP-binding protein EcfA1
Gene
ecfA1, cbiO, ecfA', TM_1663
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates Inferred. Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei157 – 1571Proton acceptor Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 436ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. ATPase activity Source: InterPro
  3. riboflavin transporter activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. riboflavin transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Energy-coupling factor transporter ATP-binding protein EcfA1 (EC:3.6.3.-)
Short name:
ECF transporter A component EcfA1
Gene namesi
Name:ecfA1
Synonyms:cbiO, ecfA'
Ordered Locus Names:TM_1663
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cell inner membrane; Peripheral membrane protein Inferred 1 Publication

GO - Cellular componenti

  1. ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Energy-coupling factor transporter ATP-binding protein EcfA1
PRO_0000092116Add
BLAST

Interactioni

Subunit structurei

Forms a heterodimer with EcfA2. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1663.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 1810
Beta strandi30 – 345
Helixi41 – 499
Helixi67 – 737
Beta strandi74 – 774
Beta strandi79 – 813
Turni82 – 843
Helixi90 – 956
Beta strandi98 – 1014
Helixi108 – 11811
Helixi129 – 1313
Helixi134 – 14613
Beta strandi151 – 1577
Turni158 – 1614
Helixi164 – 17815
Turni179 – 1813
Beta strandi183 – 1875
Helixi191 – 1933
Beta strandi199 – 2046
Beta strandi207 – 2126
Helixi214 – 2207
Beta strandi223 – 2253
Helixi229 – 2379

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HLUX-ray2.70C/D2-259[»]
ProteinModelPortaliQ9X1Z1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 230228ABC transporter
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1122.
KOiK16786.
OMAiDESEMRK.
OrthoDBiEOG6T7N3V.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015856. ABC_transpr_CbiO/EcfA_su.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51246. CBIO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1Z1-1 [UniParc]FASTAAdd to Basket

« Hide

MKITLNSVSF RYNGDYVLKD VNAEFETGKI YVVVGKNGSG KTTLLKILAG    50
LLEAEGEIFL DGSPADPFLL RKNVGYVFQN PSSQIIGATV EEDVAFSLEI 100
MGLDESEMRK RIKKVLELVG LSGLEKEDPL NLSGGQKQRL AIASMLARDT 150
RFLALDEPVS MLDPPSQREI FQVLESLKNE GKGIILVTHE LEYLDDMDFI 200
LHISNGTIDF CGSWEEFVER EFDDVEIPFK WKLWKKCGKI NLWEDRYENS 250
GNQRRRDTV 259
Length:259
Mass (Da):29,376
Last modified:November 1, 1999 - v1
Checksum:i80B492FFF04A2C6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36730.1.
PIRiE72224.
RefSeqiNP_229463.1. NC_000853.1.
WP_004082181.1. NC_023151.1.
YP_007978021.1. NC_021214.1.
YP_008991243.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD36730; AAD36730; TM_1663.
GeneIDi897908.
KEGGitma:TM1663.
tmi:THEMA_05930.
tmm:Tmari_1672.
PATRICi23938300. VBITheMar51294_1682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36730.1 .
PIRi E72224.
RefSeqi NP_229463.1. NC_000853.1.
WP_004082181.1. NC_023151.1.
YP_007978021.1. NC_021214.1.
YP_008991243.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HLU X-ray 2.70 C/D 2-259 [» ]
ProteinModelPortali Q9X1Z1.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243274.TM1663.

Protein family/group databases

TCDBi 3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

DNASUi 897908.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36730 ; AAD36730 ; TM_1663 .
GeneIDi 897908.
KEGGi tma:TM1663.
tmi:THEMA_05930.
tmm:Tmari_1672.
PATRICi 23938300. VBITheMar51294_1682.

Phylogenomic databases

eggNOGi COG1122.
KOi K16786.
OMAi DESEMRK.
OrthoDBi EOG6T7N3V.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015856. ABC_transpr_CbiO/EcfA_su.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00005. ABC_tran. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51246. CBIO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Assembly and mechanism of a group II ECF transporter."
    Karpowich N.K., Wang D.N.
    Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, EXPRESSION IN E.COLI, ATP-BINDING, X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 5-259 IN COMPLEX WITH ADP.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiECFA1_THEMA
AccessioniPrimary (citable) accession number: Q9X1Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Structure 4HLU is probably in the open state.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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