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Protein

Quinolinate synthase A

Gene

nadA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA), Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191IminoaspartateUniRule annotation
Binding sitei36 – 361Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei107 – 1071IminoaspartateUniRule annotation
Binding sitei124 – 1241IminoaspartateUniRule annotation
Binding sitei193 – 1931IminoaspartateUniRule annotation
Binding sitei210 – 2101IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:TM_1644
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Quinolinate synthase APRO_0000155798Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1644.

Structurei

Secondary structure

298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1212Combined sources
Beta strandi14 – 196Combined sources
Helixi24 – 296Combined sources
Beta strandi31 – 344Combined sources
Helixi36 – 438Combined sources
Beta strandi50 – 556Combined sources
Helixi57 – 6610Combined sources
Beta strandi70 – 734Combined sources
Helixi82 – 854Combined sources
Helixi89 – 9810Combined sources
Beta strandi104 – 1107Combined sources
Helixi112 – 1154Combined sources
Beta strandi119 – 1224Combined sources
Turni124 – 1263Combined sources
Helixi127 – 1337Combined sources
Beta strandi137 – 1437Combined sources
Helixi145 – 15511Combined sources
Beta strandi158 – 1636Combined sources
Helixi169 – 1713Combined sources
Helixi175 – 18410Combined sources
Beta strandi189 – 1924Combined sources
Helixi198 – 2036Combined sources
Beta strandi204 – 2074Combined sources
Helixi210 – 21910Combined sources
Beta strandi223 – 2297Combined sources
Helixi232 – 2409Combined sources
Beta strandi244 – 2507Combined sources
Helixi257 – 2593Combined sources
Helixi262 – 27110Combined sources
Helixi280 – 29617Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P3XX-ray1.65A1-298[»]
ProteinModelPortaliQ9X1X7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
InParanoidiQ9X1X7.
KOiK03517.
OMAiIAHPECE.
OrthoDBiEOG6DJZ56.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X1X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDEILKLKK EKGYIILAHN YQIPELQDIA DFVGDSLQLA RKAMELSEKK
60 70 80 90 100
ILFLGVDFMA ELVKILNPDK KVIVPDRSAT CPMANRLTPE IIREYREKFP
110 120 130 140 150
DAPVVLYVNS TSECKTLADV ICTSANAVEV VKKLDSSVVI FGPDRNLGEY
160 170 180 190 200
VAEKTGKKVI TIPENGHCPV HQFNAESIDA VRKKYPDAKV IVHPECPKPV
210 220 230 240 250
RDKADYVGST GQMEKIPEKD PSRIFVIGTE IGMIHKLKKK FPDREFVPLE
260 270 280 290
MAVCVNMKKN TLENTLHALQ TESFEVILPK EVIEKAKKPI LRMFELMG
Length:298
Mass (Da):33,617
Last modified:November 1, 1999 - v1
Checksum:i682510831BFF6D01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36711.1.
PIRiA72227.
RefSeqiNP_229444.1. NC_000853.1.
WP_004082140.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36711; AAD36711; TM_1644.
GeneIDi897919.
KEGGitma:TM1644.
PATRICi23938262. VBITheMar51294_1663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36711.1.
PIRiA72227.
RefSeqiNP_229444.1. NC_000853.1.
WP_004082140.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P3XX-ray1.65A1-298[»]
ProteinModelPortaliQ9X1X7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36711; AAD36711; TM_1644.
GeneIDi897919.
KEGGitma:TM1644.
PATRICi23938262. VBITheMar51294_1663.

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
InParanoidiQ9X1X7.
KOiK03517.
OMAiIAHPECE.
OrthoDBiEOG6DJZ56.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiNADA_THEMA
AccessioniPrimary (citable) accession number: Q9X1X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: November 1, 1999
Last modified: July 6, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.