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Protein

Quinolinate synthase A

Gene

nadA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA), Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19IminoaspartateUniRule annotation1
Binding sitei36Iminoaspartate; via amide nitrogenUniRule annotation1
Binding sitei107IminoaspartateUniRule annotation1
Binding sitei124IminoaspartateUniRule annotation1
Binding sitei193IminoaspartateUniRule annotation1
Binding sitei210IminoaspartateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:TM_1644
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001557981 – 298Quinolinate synthase AAdd BLAST298

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1644.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 12Combined sources12
Beta strandi14 – 19Combined sources6
Helixi24 – 27Combined sources4
Beta strandi31 – 34Combined sources4
Helixi36 – 45Combined sources10
Beta strandi49 – 55Combined sources7
Helixi57 – 66Combined sources10
Beta strandi70 – 73Combined sources4
Helixi82 – 84Combined sources3
Helixi89 – 98Combined sources10
Beta strandi104 – 110Combined sources7
Helixi112 – 115Combined sources4
Beta strandi119 – 122Combined sources4
Turni124 – 126Combined sources3
Helixi127 – 133Combined sources7
Beta strandi137 – 143Combined sources7
Helixi145 – 155Combined sources11
Beta strandi158 – 161Combined sources4
Helixi169 – 171Combined sources3
Helixi176 – 184Combined sources9
Beta strandi189 – 192Combined sources4
Helixi198 – 203Combined sources6
Beta strandi204 – 207Combined sources4
Helixi210 – 215Combined sources6
Helixi216 – 218Combined sources3
Beta strandi223 – 229Combined sources7
Helixi232 – 240Combined sources9
Beta strandi244 – 250Combined sources7
Helixi257 – 259Combined sources3
Helixi262 – 271Combined sources10
Helixi280 – 297Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P3XX-ray1.65A1-298[»]
5F33X-ray1.45A1-298[»]
5F35X-ray1.60A1-298[»]
5F3DX-ray1.90A1-298[»]
5LQMX-ray1.62A1-298[»]
5LQSX-ray1.90A1-298[»]
ProteinModelPortaliQ9X1X7.
SMRiQ9X1X7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
InParanoidiQ9X1X7.
KOiK03517.
OMAiIAHPECE.

Family and domain databases

HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X1X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDEILKLKK EKGYIILAHN YQIPELQDIA DFVGDSLQLA RKAMELSEKK
60 70 80 90 100
ILFLGVDFMA ELVKILNPDK KVIVPDRSAT CPMANRLTPE IIREYREKFP
110 120 130 140 150
DAPVVLYVNS TSECKTLADV ICTSANAVEV VKKLDSSVVI FGPDRNLGEY
160 170 180 190 200
VAEKTGKKVI TIPENGHCPV HQFNAESIDA VRKKYPDAKV IVHPECPKPV
210 220 230 240 250
RDKADYVGST GQMEKIPEKD PSRIFVIGTE IGMIHKLKKK FPDREFVPLE
260 270 280 290
MAVCVNMKKN TLENTLHALQ TESFEVILPK EVIEKAKKPI LRMFELMG
Length:298
Mass (Da):33,617
Last modified:November 1, 1999 - v1
Checksum:i682510831BFF6D01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36711.1.
PIRiA72227.
RefSeqiNP_229444.1. NC_000853.1.
WP_004082140.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36711; AAD36711; TM_1644.
GeneIDi897919.
KEGGitma:TM1644.
PATRICi23938262. VBITheMar51294_1663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36711.1.
PIRiA72227.
RefSeqiNP_229444.1. NC_000853.1.
WP_004082140.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P3XX-ray1.65A1-298[»]
5F33X-ray1.45A1-298[»]
5F35X-ray1.60A1-298[»]
5F3DX-ray1.90A1-298[»]
5LQMX-ray1.62A1-298[»]
5LQSX-ray1.90A1-298[»]
ProteinModelPortaliQ9X1X7.
SMRiQ9X1X7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36711; AAD36711; TM_1644.
GeneIDi897919.
KEGGitma:TM1644.
PATRICi23938262. VBITheMar51294_1663.

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
InParanoidiQ9X1X7.
KOiK03517.
OMAiIAHPECE.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.

Family and domain databases

HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADA_THEMA
AccessioniPrimary (citable) accession number: Q9X1X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.