Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9X1P7 (TGT_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:TM_1561
OrganismThermotoga maritima
Taxonomic identifier2336 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_0000135545

Sites

Active site891Nucleophile By similarity
Metal binding2991Zinc By similarity
Metal binding3011Zinc By similarity
Metal binding3041Zinc By similarity
Metal binding3301Zinc By similarity
Binding site901Substrate By similarity

Secondary structure

1 ................................................................. 369
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9X1P7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 5E9FF9C589347DF2

FASTA36941,429
        10         20         30         40         50         60 
MEFEVKKTFG KARLGVMKLH HGAVETPVFM PVGTNASVKL LTPRDLEEAG AEIILSNTFH 

        70         80         90        100        110        120 
LMLKPGVEII KLHRGLHNFM GWKRPILTDS GGFQVFSLPK IRIDDEGVVF RSPIDGSKVF 

       130        140        150        160        170        180 
LNPEISMEVQ IALGSDICMV FDHCPVPDAD YEEVKEATER TYRWALRSKK AFKTENQALF 

       190        200        210        220        230        240 
GIVQGGIYPD LRRESALQLT SIGFDGYAIG GLSIGEERSL TLEMTEVTVE FLPEDKPRYF 

       250        260        270        280        290        300 
MGGGSPELIL ELVDRGVDMF DSVFPTRIAR HGTALTWNGK LNLKASYNKR SLEPVDERCG 

       310        320        330        340        350        360 
CYTCKNFTRS YIHHLFDRGE VLGQILLTIH NINFMISLMK EVRRSIESGT FKELKSKVVE 


VYSSGGVNV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000512 Genomic DNA. Translation: AAD36627.1.
PIRD72240.
RefSeqNP_229361.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ASHX-ray1.90A/B/C/D1-369[»]
ProteinModelPortalQ9X1P7.
SMRQ9X1P7. Positions 1-363.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID897381.
GenomeReviewsGene locus TM_1561 in contig AE000512_GR.
KEGGtma:TM1561.
NMPDRfig|243274.1.peg.1545.
PATRIC23938084. VBITheMar51294_1579.
TIGRTM_1561.

Phylogenomic databases

HOGENOMHBG629929.
OMAFMPVGTV.
PhylomeDBQ9X1P7.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycTMAR243274:TM_1561-MONOMER.

Family and domain databases

HAMAPMF_00168. Q_tRNA_Tgt.
[Tree]
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KOK00773.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. Tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_THEMA
AccessionPrimary (citable) accession number: Q9X1P7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents