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Protein

Pseudouridine-5'-phosphate glycosidase

Gene

psuG

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil.1 Publication
Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.UniRule annotation1 Publication

Catalytic activityi

Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O.UniRule annotation1 Publication

Cofactori

Mn2+1 PublicationUniRule annotation1 PublicationNote: Binds 1 Mn2+ ion per subunit.1 PublicationUniRule annotation1 Publication

Temperature dependencei

No activity can be observed at or below 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei17 – 171Proton donorUniRule annotation
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei97 – 971Substrate; via amide nitrogenUniRule annotation
Metal bindingi126 – 1261ManganeseUniRule annotation1 Publication
Active sitei147 – 1471NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pseudouridine-5'-phosphate glycosidase1 PublicationUniRule annotation (EC:4.2.1.70UniRule annotation1 Publication)
Short name:
PsiMP glycosidase1 PublicationUniRule annotation
Gene namesi
Name:psuGUniRule annotation
Synonyms:indA1 Publication
Ordered Locus Names:TM_1464
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Pseudouridine-5'-phosphate glycosidasePRO_0000390555Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243274.TM1464.

Structurei

Secondary structure

1
285
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Beta strandi13 – 164Combined sources
Helixi19 – 224Combined sources
Helixi27 – 4418Combined sources
Beta strandi47 – 548Combined sources
Beta strandi57 – 615Combined sources
Helixi64 – 7310Combined sources
Beta strandi76 – 783Combined sources
Helixi80 – 823Combined sources
Helixi83 – 897Combined sources
Beta strandi93 – 953Combined sources
Helixi97 – 10610Combined sources
Beta strandi111 – 1133Combined sources
Helixi129 – 1346Combined sources
Beta strandi139 – 1468Combined sources
Helixi152 – 16110Combined sources
Beta strandi166 – 1705Combined sources
Helixi192 – 20413Combined sources
Beta strandi209 – 2146Combined sources
Helixi219 – 2213Combined sources
Helixi225 – 2339Combined sources
Helixi241 – 2433Combined sources
Helixi244 – 25512Combined sources
Turni256 – 2583Combined sources
Helixi259 – 28224Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VKMX-ray1.90A/B/C/D/E/F1-285[»]
ProteinModelPortaliQ9X1H5.
SMRiQ9X1H5. Positions 1-284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X1H5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 1303Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the pseudouridine-5'-phosphate glycosidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D53. Bacteria.
COG2313. LUCA.
InParanoidiQ9X1H5.
KOiK16329.
OMAiIIAHGMP.

Family and domain databases

HAMAPiMF_01876. PsiMP_glycosidase. 1 hit.
InterProiIPR007342. PsuG.
[Graphical view]
PfamiPF04227. Indigoidine_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIESRIEKG KPVVGMETTV FVHGLPRKEA IELFRRAKEI SREKGFQLAV
60 70 80 90 100
IGILKGKIVA GMSEEELEAM MREGADKVGT REIPIVVAEG KNAATTVSAT
110 120 130 140 150
IFLSRRIGIE VVVTGGTGGV HPGRVDVSQD LTEMSSSRAV LVSSGIKSIL
160 170 180 190 200
DVEATFEMLE TLEIPLVGFR TNEFPLFFSR KSGRRVPRIE NVEEVLKIYE
210 220 230 240 250
SMKEMELEKT LMVLNPVPEE YEIPHDEIER LLEKIELEVE GKEVTPFLLK
260 270 280
KLVEMTNGRT LKANLALLEE NVKLAGEIAV KLKRS
Length:285
Mass (Da):31,728
Last modified:November 1, 1999 - v1
Checksum:i9657B169EC62DB74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36532.1.
PIRiA72252.
RefSeqiNP_229264.1. NC_000853.1.
WP_004081760.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36532; AAD36532; TM_1464.
GeneIDi896983.
KEGGitma:TM1464.
tmi:THEMA_06990.
tmw:THMA_1495.
PATRICi23937882. VBITheMar51294_1479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36532.1.
PIRiA72252.
RefSeqiNP_229264.1. NC_000853.1.
WP_004081760.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VKMX-ray1.90A/B/C/D/E/F1-285[»]
ProteinModelPortaliQ9X1H5.
SMRiQ9X1H5. Positions 1-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36532; AAD36532; TM_1464.
GeneIDi896983.
KEGGitma:TM1464.
tmi:THEMA_06990.
tmw:THMA_1495.
PATRICi23937882. VBITheMar51294_1479.

Phylogenomic databases

eggNOGiENOG4105D53. Bacteria.
COG2313. LUCA.
InParanoidiQ9X1H5.
KOiK16329.
OMAiIIAHGMP.

Miscellaneous databases

EvolutionaryTraceiQ9X1H5.

Family and domain databases

HAMAPiMF_01876. PsiMP_glycosidase. 1 hit.
InterProiIPR007342. PsuG.
[Graphical view]
PfamiPF04227. Indigoidine_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSUG_THEMA
AccessioniPrimary (citable) accession number: Q9X1H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.