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Q9X1F8

- DER_THEMA

UniProt

Q9X1F8 - DER_THEMA

Protein

GTPase Der

Gene

der

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    GTPase that plays an essential role in the late steps of ribosome biogenesis By similarity. Has GTPase activity but no ATPase activity. GTP, GDP, and dGTP but not GMP, ATP, CTP, and UTP compete for GTP binding.By similarity

    Kineticsi

    At pH 7.5, 70 degrees Celsius, 5 mM MgCl2 and 400 mM KCl.

    1. KM=110 µM for GTP1 Publication

    Vmax=0.35 µmol/min/µg enzyme1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 158GTP 1Sequence Analysis
    Nucleotide bindingi55 – 595GTP 1Sequence Analysis
    Nucleotide bindingi118 – 1214GTP 1Sequence Analysis
    Nucleotide bindingi190 – 1945GTP 2
    Nucleotide bindingi300 – 3034GTP 2
    Nucleotide bindingi336 – 3372GTP 2

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. ribosome biogenesis Source: UniProtKB-KW

    Keywords - Biological processi

    Ribosome biogenesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase Der
    Short name:
    TmDer
    Alternative name(s):
    GTP-binding protein EngA
    Gene namesi
    Name:der
    Synonyms:engA
    Ordered Locus Names:TM_1446
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi118 – 1181N → D: 10% of GTPase activity remains. 1 Publication
    Mutagenesisi300 – 3001N → D: Slight increase of GTPase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 439439GTPase DerPRO_0000179064Add
    BLAST

    Interactioni

    Subunit structurei

    Associates with the 50S ribosomal subunit.By similarity

    Protein-protein interaction databases

    STRINGi243274.TM1446.

    Structurei

    Secondary structure

    1
    439
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Helixi14 – 229
    Beta strandi40 – 467
    Beta strandi49 – 557
    Turni57 – 604
    Helixi63 – 653
    Helixi69 – 7911
    Beta strandi83 – 908
    Turni91 – 933
    Helixi97 – 10913
    Beta strandi113 – 1197
    Helixi123 – 1297
    Helixi131 – 1344
    Helixi135 – 1373
    Turni147 – 1504
    Helixi153 – 16614
    Beta strandi171 – 1733
    Beta strandi181 – 1866
    Helixi193 – 2019
    Beta strandi206 – 2083
    Beta strandi221 – 2255
    Beta strandi228 – 2347
    Helixi255 – 2639
    Beta strandi265 – 2728
    Turni273 – 2753
    Helixi279 – 2846
    Helixi288 – 2903
    Beta strandi294 – 3007
    Helixi302 – 3043
    Helixi308 – 3103
    Helixi312 – 32211
    Helixi324 – 3263
    Beta strandi331 – 3333
    Turni336 – 3394
    Helixi342 – 3454
    Helixi349 – 3568
    Helixi362 – 37312
    Beta strandi384 – 3918
    Turni392 – 3954
    Beta strandi396 – 4027
    Helixi409 – 42214
    Beta strandi432 – 4376

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MKYX-ray1.90A1-439[»]
    ProteinModelPortaliQ9X1F8.
    SMRiQ9X1F8. Positions 2-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X1F8.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 168167EngA-type G 1Add
    BLAST
    Domaini181 – 357177EngA-type G 2Add
    BLAST
    Domaini358 – 43982KH-likeAdd
    BLAST

    Domaini

    Each G (guanine nucleotide-binding) domain has activity on its own; domain 1 is twice as active as domain 2. The G domains do not interact, instead each contacts the C-terminal KH-like domain which lies between them.1 Publication

    Sequence similaritiesi

    Contains 1 KH-like domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1160.
    KOiK03977.
    OMAiSQGRGIN.
    OrthoDBiEOG6DC6K1.

    Family and domain databases

    Gene3Di3.30.300.20. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00195. GTPase_Der.
    InterProiIPR016484. GTP-bd_EngA.
    IPR006073. GTP_binding_domain.
    IPR015946. KH_dom-like_a/b.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    [Graphical view]
    PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
    PfamiPF01926. MMR_HSR1. 2 hits.
    [Graphical view]
    PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
    PRINTSiPR00326. GTP1OBG.
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
    TIGR00231. small_GTP. 2 hits.
    PROSITEiPS51712. G_ENGA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X1F8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATVLIVGRP NVGKSTLFNK LVKKKKAIVE DEEGVTRDPV QDTVEWYGKT    50
    FKLVDTCGVF DNPQDIISQK MKEVTLNMIR EADLVLFVVD GKRGITKEDE 100
    SLADFLRKST VDTILVANKA ENLREFEREV KPELYSLGFG EPIPVSAEHN 150
    INLDTLLETI IKKLEEKGLD LESKPEITDA IKVAIVGRPN VGKSTLFNAI 200
    LNKERALVSP IPGTTRDPVD DEVFIDGRKY VFVDTAGLRR KSRVEPRTVE 250
    KYSNYRVVDS IEKADVVVIV LDATQGITRQ DQRIAGLVER RGRASVVVFN 300
    KWDLVEHREK RYDEFTKLFR EKLYFIDYSP LIFTSADKGW NIDRVIDAIN 350
    LAYASYTTKV PSSAINSALQ KVLAFTNLPR GLKIFFGLQV DIKPPTFLFF 400
    VNSIEKVKNP QKIFLRKLIR DYVFPFEGSP IFLKFKRSR 439
    Length:439
    Mass (Da):50,015
    Last modified:November 1, 1999 - v1
    Checksum:i29CA321453249B58
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36514.1.
    PIRiB72253.
    RefSeqiNP_229245.1. NC_000853.1.
    YP_007977801.1. NC_021214.1.
    YP_008991465.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36514; AAD36514; TM_1446.
    GeneIDi898030.
    KEGGitma:TM1446.
    tmi:THEMA_07085.
    tmm:Tmari_1452.
    PATRICi23937844. VBITheMar51294_1460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36514.1 .
    PIRi B72253.
    RefSeqi NP_229245.1. NC_000853.1.
    YP_007977801.1. NC_021214.1.
    YP_008991465.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MKY X-ray 1.90 A 1-439 [» ]
    ProteinModelPortali Q9X1F8.
    SMRi Q9X1F8. Positions 2-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1446.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36514 ; AAD36514 ; TM_1446 .
    GeneIDi 898030.
    KEGGi tma:TM1446.
    tmi:THEMA_07085.
    tmm:Tmari_1452.
    PATRICi 23937844. VBITheMar51294_1460.

    Phylogenomic databases

    eggNOGi COG1160.
    KOi K03977.
    OMAi SQGRGIN.
    OrthoDBi EOG6DC6K1.

    Miscellaneous databases

    EvolutionaryTracei Q9X1F8.

    Family and domain databases

    Gene3Di 3.30.300.20. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00195. GTPase_Der.
    InterProi IPR016484. GTP-bd_EngA.
    IPR006073. GTP_binding_domain.
    IPR015946. KH_dom-like_a/b.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11649:SF5. PTHR11649:SF5. 1 hit.
    Pfami PF01926. MMR_HSR1. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF006485. GTP-binding_EngA. 1 hit.
    PRINTSi PR00326. GTP1OBG.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR03594. GTPase_EngA. 1 hit.
    TIGR00231. small_GTP. 2 hits.
    PROSITEi PS51712. G_ENGA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "An essential GTPase, der, containing double GTP-binding domains from Escherichia coli and Thermotoga maritima."
      Hwang J., Inouye M.
      J. Biol. Chem. 276:31415-31421(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
    3. "Domain arrangement of Der, a switch protein containing two GTPase domains."
      Robinson V.L., Hwang J., Fox E., Inouye M., Stock A.M.
      Structure 10:1649-1658(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH GDP, GTPASE ACTIVITY, GTP-BINDING, DOMAINS, MUTAGENESIS OF ASN-118 AND ASN-300.

    Entry informationi

    Entry nameiDER_THEMA
    AccessioniPrimary (citable) accession number: Q9X1F8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3