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Q9X1F8

- DER_THEMA

UniProt

Q9X1F8 - DER_THEMA

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Protein

GTPase Der

Gene
der, engA, TM_1446
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase that plays an essential role in the late steps of ribosome biogenesis By similarity. Has GTPase activity but no ATPase activity. GTP, GDP, and dGTP but not GMP, ATP, CTP, and UTP compete for GTP binding.UniRule annotation

Kineticsi

At pH 7.5, 70 degrees Celsius, 5 mM MgCl2 and 400 mM KCl.

  1. KM=110 µM for GTP1 Publication

Vmax=0.35 µmol/min/µg enzyme

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158GTP 1 Reviewed prediction
Nucleotide bindingi55 – 595GTP 1 Reviewed prediction
Nucleotide bindingi118 – 1214GTP 1 Reviewed prediction
Nucleotide bindingi190 – 1945GTP 2UniRule annotation
Nucleotide bindingi300 – 3034GTP 2UniRule annotation
Nucleotide bindingi336 – 3372GTP 2UniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. ribosome biogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Der
Short name:
TmDer
Alternative name(s):
GTP-binding protein EngA
Gene namesi
Name:der
Synonyms:engA
Ordered Locus Names:TM_1446
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181N → D: 10% of GTPase activity remains. 1 Publication
Mutagenesisi300 – 3001N → D: Slight increase of GTPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439GTPase DerUniRule annotationPRO_0000179064Add
BLAST

Interactioni

Subunit structurei

Associates with the 50S ribosomal subunit By similarity.

Protein-protein interaction databases

STRINGi243274.TM1446.

Structurei

Secondary structure

1
439
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75
Helixi14 – 229
Beta strandi40 – 467
Beta strandi49 – 557
Turni57 – 604
Helixi63 – 653
Helixi69 – 7911
Beta strandi83 – 908
Turni91 – 933
Helixi97 – 10913
Beta strandi113 – 1197
Helixi123 – 1297
Helixi131 – 1344
Helixi135 – 1373
Turni147 – 1504
Helixi153 – 16614
Beta strandi171 – 1733
Beta strandi181 – 1866
Helixi193 – 2019
Beta strandi206 – 2083
Beta strandi221 – 2255
Beta strandi228 – 2347
Helixi255 – 2639
Beta strandi265 – 2728
Turni273 – 2753
Helixi279 – 2846
Helixi288 – 2903
Beta strandi294 – 3007
Helixi302 – 3043
Helixi308 – 3103
Helixi312 – 32211
Helixi324 – 3263
Beta strandi331 – 3333
Turni336 – 3394
Helixi342 – 3454
Helixi349 – 3568
Helixi362 – 37312
Beta strandi384 – 3918
Turni392 – 3954
Beta strandi396 – 4027
Helixi409 – 42214
Beta strandi432 – 4376

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKYX-ray1.90A1-439[»]
ProteinModelPortaliQ9X1F8.
SMRiQ9X1F8. Positions 2-439.

Miscellaneous databases

EvolutionaryTraceiQ9X1F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 168167EngA-type G 1Add
BLAST
Domaini181 – 357177EngA-type G 2Add
BLAST
Domaini358 – 43982KH-likeAdd
BLAST

Domaini

Each G (guanine nucleotide-binding) domain has activity on its own; domain 1 is twice as active as domain 2. The G domains do not interact, instead each contacts the C-terminal KH-like domain which lies between them.1 Publication

Sequence similaritiesi

Contains 1 KH-like domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1160.
KOiK03977.
OMAiSQGRGIN.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00195. GTPase_Der.
InterProiIPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
PfamiPF01926. MMR_HSR1. 2 hits.
[Graphical view]
PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEiPS51712. G_ENGA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1F8-1 [UniParc]FASTAAdd to Basket

« Hide

MATVLIVGRP NVGKSTLFNK LVKKKKAIVE DEEGVTRDPV QDTVEWYGKT    50
FKLVDTCGVF DNPQDIISQK MKEVTLNMIR EADLVLFVVD GKRGITKEDE 100
SLADFLRKST VDTILVANKA ENLREFEREV KPELYSLGFG EPIPVSAEHN 150
INLDTLLETI IKKLEEKGLD LESKPEITDA IKVAIVGRPN VGKSTLFNAI 200
LNKERALVSP IPGTTRDPVD DEVFIDGRKY VFVDTAGLRR KSRVEPRTVE 250
KYSNYRVVDS IEKADVVVIV LDATQGITRQ DQRIAGLVER RGRASVVVFN 300
KWDLVEHREK RYDEFTKLFR EKLYFIDYSP LIFTSADKGW NIDRVIDAIN 350
LAYASYTTKV PSSAINSALQ KVLAFTNLPR GLKIFFGLQV DIKPPTFLFF 400
VNSIEKVKNP QKIFLRKLIR DYVFPFEGSP IFLKFKRSR 439
Length:439
Mass (Da):50,015
Last modified:November 1, 1999 - v1
Checksum:i29CA321453249B58
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36514.1.
PIRiB72253.
RefSeqiNP_229245.1. NC_000853.1.
YP_007977801.1. NC_021214.1.
YP_008991465.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD36514; AAD36514; TM_1446.
GeneIDi898030.
KEGGitma:TM1446.
tmi:THEMA_07085.
tmm:Tmari_1452.
PATRICi23937844. VBITheMar51294_1460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36514.1 .
PIRi B72253.
RefSeqi NP_229245.1. NC_000853.1.
YP_007977801.1. NC_021214.1.
YP_008991465.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MKY X-ray 1.90 A 1-439 [» ]
ProteinModelPortali Q9X1F8.
SMRi Q9X1F8. Positions 2-439.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM1446.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36514 ; AAD36514 ; TM_1446 .
GeneIDi 898030.
KEGGi tma:TM1446.
tmi:THEMA_07085.
tmm:Tmari_1452.
PATRICi 23937844. VBITheMar51294_1460.

Phylogenomic databases

eggNOGi COG1160.
KOi K03977.
OMAi SQGRGIN.
OrthoDBi EOG6DC6K1.

Miscellaneous databases

EvolutionaryTracei Q9X1F8.

Family and domain databases

Gene3Di 3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00195. GTPase_Der.
InterProi IPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view ]
PANTHERi PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfami PF01926. MMR_HSR1. 2 hits.
[Graphical view ]
PIRSFi PIRSF006485. GTP-binding_EngA. 1 hit.
PRINTSi PR00326. GTP1OBG.
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEi PS51712. G_ENGA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "An essential GTPase, der, containing double GTP-binding domains from Escherichia coli and Thermotoga maritima."
    Hwang J., Inouye M.
    J. Biol. Chem. 276:31415-31421(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
  3. "Domain arrangement of Der, a switch protein containing two GTPase domains."
    Robinson V.L., Hwang J., Fox E., Inouye M., Stock A.M.
    Structure 10:1649-1658(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH GDP, GTPASE ACTIVITY, GTP-BINDING, DOMAINS, MUTAGENESIS OF ASN-118 AND ASN-300.

Entry informationi

Entry nameiDER_THEMA
AccessioniPrimary (citable) accession number: Q9X1F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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