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Q9X1F8

- DER_THEMA

UniProt

Q9X1F8 - DER_THEMA

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Protein

GTPase Der

Gene

der

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity). Has GTPase activity but no ATPase activity. GTP, GDP, and dGTP but not GMP, ATP, CTP, and UTP compete for GTP binding.By similarity

Kineticsi

At pH 7.5, 70 degrees Celsius, 5 mM MgCl2 and 400 mM KCl.

  1. KM=110 µM for GTP1 Publication

Vmax=0.35 µmol/min/µg enzyme1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158GTP 1Sequence Analysis
Nucleotide bindingi55 – 595GTP 1Sequence Analysis
Nucleotide bindingi118 – 1214GTP 1Sequence Analysis
Nucleotide bindingi190 – 1945GTP 2
Nucleotide bindingi300 – 3034GTP 2
Nucleotide bindingi336 – 3372GTP 2

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. ribosome biogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Der
Short name:
TmDer
Alternative name(s):
GTP-binding protein EngA
Gene namesi
Name:der
Synonyms:engA
Ordered Locus Names:TM_1446
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181N → D: 10% of GTPase activity remains. 1 Publication
Mutagenesisi300 – 3001N → D: Slight increase of GTPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439GTPase DerPRO_0000179064Add
BLAST

Interactioni

Subunit structurei

Associates with the 50S ribosomal subunit.By similarity

Protein-protein interaction databases

STRINGi243274.TM1446.

Structurei

Secondary structure

1
439
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi14 – 229Combined sources
Beta strandi40 – 467Combined sources
Beta strandi49 – 557Combined sources
Turni57 – 604Combined sources
Helixi63 – 653Combined sources
Helixi69 – 7911Combined sources
Beta strandi83 – 908Combined sources
Turni91 – 933Combined sources
Helixi97 – 10913Combined sources
Beta strandi113 – 1197Combined sources
Helixi123 – 1297Combined sources
Helixi131 – 1344Combined sources
Helixi135 – 1373Combined sources
Turni147 – 1504Combined sources
Helixi153 – 16614Combined sources
Beta strandi171 – 1733Combined sources
Beta strandi181 – 1866Combined sources
Helixi193 – 2019Combined sources
Beta strandi206 – 2083Combined sources
Beta strandi221 – 2255Combined sources
Beta strandi228 – 2347Combined sources
Helixi255 – 2639Combined sources
Beta strandi265 – 2728Combined sources
Turni273 – 2753Combined sources
Helixi279 – 2846Combined sources
Helixi288 – 2903Combined sources
Beta strandi294 – 3007Combined sources
Helixi302 – 3043Combined sources
Helixi308 – 3103Combined sources
Helixi312 – 32211Combined sources
Helixi324 – 3263Combined sources
Beta strandi331 – 3333Combined sources
Turni336 – 3394Combined sources
Helixi342 – 3454Combined sources
Helixi349 – 3568Combined sources
Helixi362 – 37312Combined sources
Beta strandi384 – 3918Combined sources
Turni392 – 3954Combined sources
Beta strandi396 – 4027Combined sources
Helixi409 – 42214Combined sources
Beta strandi432 – 4376Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKYX-ray1.90A1-439[»]
ProteinModelPortaliQ9X1F8.
SMRiQ9X1F8. Positions 2-439.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X1F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 168167EngA-type G 1Add
BLAST
Domaini181 – 357177EngA-type G 2Add
BLAST
Domaini358 – 43982KH-likeAdd
BLAST

Domaini

Each G (guanine nucleotide-binding) domain has activity on its own; domain 1 is twice as active as domain 2. The G domains do not interact, instead each contacts the C-terminal KH-like domain which lies between them.1 Publication

Sequence similaritiesi

Contains 1 KH-like domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1160.
InParanoidiQ9X1F8.
KOiK03977.
OMAiSQGRGIN.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00195. GTPase_Der.
InterProiIPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
PfamiPF01926. MMR_HSR1. 2 hits.
[Graphical view]
PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEiPS51712. G_ENGA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1F8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATVLIVGRP NVGKSTLFNK LVKKKKAIVE DEEGVTRDPV QDTVEWYGKT
60 70 80 90 100
FKLVDTCGVF DNPQDIISQK MKEVTLNMIR EADLVLFVVD GKRGITKEDE
110 120 130 140 150
SLADFLRKST VDTILVANKA ENLREFEREV KPELYSLGFG EPIPVSAEHN
160 170 180 190 200
INLDTLLETI IKKLEEKGLD LESKPEITDA IKVAIVGRPN VGKSTLFNAI
210 220 230 240 250
LNKERALVSP IPGTTRDPVD DEVFIDGRKY VFVDTAGLRR KSRVEPRTVE
260 270 280 290 300
KYSNYRVVDS IEKADVVVIV LDATQGITRQ DQRIAGLVER RGRASVVVFN
310 320 330 340 350
KWDLVEHREK RYDEFTKLFR EKLYFIDYSP LIFTSADKGW NIDRVIDAIN
360 370 380 390 400
LAYASYTTKV PSSAINSALQ KVLAFTNLPR GLKIFFGLQV DIKPPTFLFF
410 420 430
VNSIEKVKNP QKIFLRKLIR DYVFPFEGSP IFLKFKRSR
Length:439
Mass (Da):50,015
Last modified:November 1, 1999 - v1
Checksum:i29CA321453249B58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36514.1.
PIRiB72253.
RefSeqiNP_229245.1. NC_000853.1.
YP_007977801.1. NC_021214.1.
YP_008991465.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD36514; AAD36514; TM_1446.
GeneIDi18093264.
898030.
KEGGitma:TM1446.
PATRICi23937844. VBITheMar51294_1460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36514.1 .
PIRi B72253.
RefSeqi NP_229245.1. NC_000853.1.
YP_007977801.1. NC_021214.1.
YP_008991465.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MKY X-ray 1.90 A 1-439 [» ]
ProteinModelPortali Q9X1F8.
SMRi Q9X1F8. Positions 2-439.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM1446.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36514 ; AAD36514 ; TM_1446 .
GeneIDi 18093264.
898030.
KEGGi tma:TM1446.
PATRICi 23937844. VBITheMar51294_1460.

Phylogenomic databases

eggNOGi COG1160.
InParanoidi Q9X1F8.
KOi K03977.
OMAi SQGRGIN.
OrthoDBi EOG6DC6K1.

Miscellaneous databases

EvolutionaryTracei Q9X1F8.

Family and domain databases

Gene3Di 3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00195. GTPase_Der.
InterProi IPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view ]
PANTHERi PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfami PF01926. MMR_HSR1. 2 hits.
[Graphical view ]
PIRSFi PIRSF006485. GTP-binding_EngA. 1 hit.
PRINTSi PR00326. GTP1OBG.
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEi PS51712. G_ENGA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "An essential GTPase, der, containing double GTP-binding domains from Escherichia coli and Thermotoga maritima."
    Hwang J., Inouye M.
    J. Biol. Chem. 276:31415-31421(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
  3. "Domain arrangement of Der, a switch protein containing two GTPase domains."
    Robinson V.L., Hwang J., Fox E., Inouye M., Stock A.M.
    Structure 10:1649-1658(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH GDP, GTPASE ACTIVITY, GTP-BINDING, DOMAINS, MUTAGENESIS OF ASN-118 AND ASN-300.

Entry informationi

Entry nameiDER_THEMA
AccessioniPrimary (citable) accession number: Q9X1F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3