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Protein

GTPase Der

Gene

der

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity). Has GTPase activity but no ATPase activity. GTP, GDP, and dGTP but not GMP, ATP, CTP, and UTP compete for GTP binding.By similarity

Kineticsi

At pH 7.5, 70 degrees Celsius, 5 mM MgCl2 and 400 mM KCl.

  1. KM=110 µM for GTP1 Publication
  1. Vmax=0.35 µmol/min/µg enzyme1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15GTP 1Sequence analysis8
Nucleotide bindingi55 – 59GTP 1Sequence analysis5
Nucleotide bindingi118 – 121GTP 1Sequence analysis4
Nucleotide bindingi190 – 194GTP 25
Nucleotide bindingi300 – 303GTP 24
Nucleotide bindingi336 – 337GTP 22

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Der
Short name:
TmDer
Alternative name(s):
GTP-binding protein EngA
Gene namesi
Name:der
Synonyms:engA
Ordered Locus Names:TM_1446
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118N → D: 10% of GTPase activity remains. 1 Publication1
Mutagenesisi300N → D: Slight increase of GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001790641 – 439GTPase DerAdd BLAST439

Interactioni

Subunit structurei

Associates with the 50S ribosomal subunit.By similarity

Protein-protein interaction databases

STRINGi243274.TM1446.

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi14 – 22Combined sources9
Beta strandi40 – 46Combined sources7
Beta strandi49 – 55Combined sources7
Turni57 – 60Combined sources4
Helixi63 – 65Combined sources3
Helixi69 – 79Combined sources11
Beta strandi83 – 90Combined sources8
Turni91 – 93Combined sources3
Helixi97 – 109Combined sources13
Beta strandi113 – 119Combined sources7
Helixi123 – 129Combined sources7
Helixi131 – 134Combined sources4
Helixi135 – 137Combined sources3
Turni147 – 150Combined sources4
Helixi153 – 166Combined sources14
Beta strandi171 – 173Combined sources3
Beta strandi181 – 186Combined sources6
Helixi193 – 201Combined sources9
Beta strandi206 – 208Combined sources3
Beta strandi221 – 225Combined sources5
Beta strandi228 – 234Combined sources7
Helixi255 – 263Combined sources9
Beta strandi265 – 272Combined sources8
Turni273 – 275Combined sources3
Helixi279 – 284Combined sources6
Helixi288 – 290Combined sources3
Beta strandi294 – 300Combined sources7
Helixi302 – 304Combined sources3
Helixi308 – 310Combined sources3
Helixi312 – 322Combined sources11
Helixi324 – 326Combined sources3
Beta strandi331 – 333Combined sources3
Turni336 – 339Combined sources4
Helixi342 – 345Combined sources4
Helixi349 – 356Combined sources8
Helixi362 – 373Combined sources12
Beta strandi384 – 391Combined sources8
Turni392 – 395Combined sources4
Beta strandi396 – 402Combined sources7
Helixi409 – 422Combined sources14
Beta strandi432 – 437Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKYX-ray1.90A2-439[»]
ProteinModelPortaliQ9X1F8.
SMRiQ9X1F8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X1F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 168EngA-type G 1Add BLAST167
Domaini181 – 357EngA-type G 2Add BLAST177
Domaini358 – 439KH-likeAdd BLAST82

Domaini

Each G (guanine nucleotide-binding) domain has activity on its own; domain 1 is twice as active as domain 2. The G domains do not interact, instead each contacts the C-terminal KH-like domain which lies between them.1 Publication

Sequence similaritiesi

Contains 1 KH-like domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DKZ. Bacteria.
COG1160. LUCA.
InParanoidiQ9X1F8.
KOiK03977.
OMAiDVMGTPI.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00195. GTPase_Der. 1 hit.
InterProiIPR031166. G_ENGA.
IPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR032859. KH_dom-like.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
PfamiPF14714. KH_dom-like. 1 hit.
PF01926. MMR_HSR1. 2 hits.
[Graphical view]
PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEiPS51712. G_ENGA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVLIVGRP NVGKSTLFNK LVKKKKAIVE DEEGVTRDPV QDTVEWYGKT
60 70 80 90 100
FKLVDTCGVF DNPQDIISQK MKEVTLNMIR EADLVLFVVD GKRGITKEDE
110 120 130 140 150
SLADFLRKST VDTILVANKA ENLREFEREV KPELYSLGFG EPIPVSAEHN
160 170 180 190 200
INLDTLLETI IKKLEEKGLD LESKPEITDA IKVAIVGRPN VGKSTLFNAI
210 220 230 240 250
LNKERALVSP IPGTTRDPVD DEVFIDGRKY VFVDTAGLRR KSRVEPRTVE
260 270 280 290 300
KYSNYRVVDS IEKADVVVIV LDATQGITRQ DQRIAGLVER RGRASVVVFN
310 320 330 340 350
KWDLVEHREK RYDEFTKLFR EKLYFIDYSP LIFTSADKGW NIDRVIDAIN
360 370 380 390 400
LAYASYTTKV PSSAINSALQ KVLAFTNLPR GLKIFFGLQV DIKPPTFLFF
410 420 430
VNSIEKVKNP QKIFLRKLIR DYVFPFEGSP IFLKFKRSR
Length:439
Mass (Da):50,015
Last modified:November 1, 1999 - v1
Checksum:i29CA321453249B58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36514.1.
PIRiB72253.
RefSeqiNP_229245.1. NC_000853.1.
WP_004081721.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36514; AAD36514; TM_1446.
GeneIDi898030.
KEGGitma:TM1446.
PATRICi23937844. VBITheMar51294_1460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36514.1.
PIRiB72253.
RefSeqiNP_229245.1. NC_000853.1.
WP_004081721.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKYX-ray1.90A2-439[»]
ProteinModelPortaliQ9X1F8.
SMRiQ9X1F8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1446.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36514; AAD36514; TM_1446.
GeneIDi898030.
KEGGitma:TM1446.
PATRICi23937844. VBITheMar51294_1460.

Phylogenomic databases

eggNOGiENOG4105DKZ. Bacteria.
COG1160. LUCA.
InParanoidiQ9X1F8.
KOiK03977.
OMAiDVMGTPI.

Miscellaneous databases

EvolutionaryTraceiQ9X1F8.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00195. GTPase_Der. 1 hit.
InterProiIPR031166. G_ENGA.
IPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR032859. KH_dom-like.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
PfamiPF14714. KH_dom-like. 1 hit.
PF01926. MMR_HSR1. 2 hits.
[Graphical view]
PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEiPS51712. G_ENGA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDER_THEMA
AccessioniPrimary (citable) accession number: Q9X1F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.