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Protein

Glycerol kinase 2

Gene

glpK2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathway: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase 1 (glpK1), Glycerol kinase 2 (glpK2), Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111SubstrateUniRule annotation
Binding sitei15 – 151ATPUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation
Binding sitei264 – 2641ATPUniRule annotation
Binding sitei307 – 3071ATP; via carbonyl oxygenUniRule annotation
Binding sitei311 – 3111ATP; via amide nitrogenUniRule annotation
Binding sitei326 – 3261ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 133ATPUniRule annotation
Nucleotide bindingi408 – 4125ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1468-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinase 2UniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferase 2UniRule annotation
Glycerokinase 2UniRule annotation
Short name:
GK 2UniRule annotation
Gene namesi
Name:glpK2UniRule annotation
Ordered Locus Names:TM_1430
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Glycerol kinase 2PRO_0000059514Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1430.

Structurei

3D structure databases

ProteinModelPortaliQ9X1E4.
SMRiQ9X1E4. Positions 2-495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 822Substrate bindingUniRule annotation
Regioni242 – 2432Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0554.
InParanoidiQ9X1E4.
KOiK00864.
OMAiGWVEHEP.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYVLSLDQG TTSSRAIVFD EKGNVVSKVN KEFRQIYPRP GWVEHDPVEI
60 70 80 90 100
WESQIEVAKK AIEEAGIKPE DIAAIGITNQ RETTIVWDKN TGKPVYNAIV
110 120 130 140 150
WQCRRTAPIC DELKEKGYSE FIRERTGLVI DAYFSGTKIK WILDNVEGVR
160 170 180 190 200
EKAEKGEVLF GTVDTWLIWN LTGGRVHVTD YSNASRTMIF NIHKLDWDDE
210 220 230 240 250
ILELLNIPRA MLPQVMPSSH VYGYTAKDIF GVEIPIAGDA GDQQAALFGQ
260 270 280 290 300
ACFQPGMLKN TYGTGCFLLM NTGEKAFESK SGLLTTIAWG INGKVYYALE
310 320 330 340 350
GSIFITGAAV QWLRDGLKII SNAAETEELA TKVPDNGGVF FVPAFVGLGA
360 370 380 390 400
PYWDMYARGL IIGITRGTTR EHIVRAVLES IAYQTRDVVE VMEKDSDIKV
410 420 430 440 450
ETLRVDGGAV VNNFLMQFQA DILGVPVERP VVNETTALGA AYLAGLAVGY
460 470 480 490
WKDQEEIASL WQLDRRFEPS MNSEERERLY SKWKEAVSRS LGWEKR
Length:496
Mass (Da):55,576
Last modified:May 30, 2000 - v2
Checksum:i7FA72A4CBD29E701
GO

Sequence cautioni

The sequence AAD36500.1 differs from that shown. Reason: Erroneous termination at position 483. Translated as Trp.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36500.1. Sequence problems.
PIRiC72254.
RefSeqiNP_229230.1. NC_000853.1.
WP_010865340.1. NC_021214.1.
YP_007977785.1. NC_021214.1.

Genome annotation databases

EnsemblBacteriaiAAD36500; AAD36500; TM_1430.
GeneIDi898045.
KEGGitma:TM1430.
tmm:Tmari_1436.
PATRICi23937808. VBITheMar51294_1442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36500.1. Sequence problems.
PIRiC72254.
RefSeqiNP_229230.1. NC_000853.1.
WP_010865340.1. NC_021214.1.
YP_007977785.1. NC_021214.1.

3D structure databases

ProteinModelPortaliQ9X1E4.
SMRiQ9X1E4. Positions 2-495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36500; AAD36500; TM_1430.
GeneIDi898045.
KEGGitma:TM1430.
tmm:Tmari_1436.
PATRICi23937808. VBITheMar51294_1442.

Phylogenomic databases

eggNOGiCOG0554.
InParanoidiQ9X1E4.
KOiK00864.
OMAiGWVEHEP.
OrthoDBiEOG6RZB46.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciTMAR243274:GC6P-1468-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiGLPK2_THEMA
AccessioniPrimary (citable) accession number: Q9X1E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: June 24, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.