Q9X1E4 (GLPK2_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase 2
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase 2
Glycerokinase 2
Short name=GK 2

Gene names

Name:	glpK2
Ordered Locus Names:	TM_1430

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.
Sequence caution	The sequence AAD36500.1 differs from that shown. Reason: Erroneous termination at position 483. Translated as Trp.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glycerol kinase 2 HAMAP MF_00186		PRO_0000059514
Regions
	Nucleotide binding	408 – 412	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	264	1	ATP By similarity
	Binding site	307	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X1E4 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 7FA72A4CBD29E701	FASTA	496	55,576
	10 20 30 40 50 60 MKYVLSLDQG TTSSRAIVFD EKGNVVSKVN KEFRQIYPRP GWVEHDPVEI WESQIEVAKK 70 80 90 100 110 120 AIEEAGIKPE DIAAIGITNQ RETTIVWDKN TGKPVYNAIV WQCRRTAPIC DELKEKGYSE 130 140 150 160 170 180 FIRERTGLVI DAYFSGTKIK WILDNVEGVR EKAEKGEVLF GTVDTWLIWN LTGGRVHVTD 190 200 210 220 230 240 YSNASRTMIF NIHKLDWDDE ILELLNIPRA MLPQVMPSSH VYGYTAKDIF GVEIPIAGDA 250 260 270 280 290 300 GDQQAALFGQ ACFQPGMLKN TYGTGCFLLM NTGEKAFESK SGLLTTIAWG INGKVYYALE 310 320 330 340 350 360 GSIFITGAAV QWLRDGLKII SNAAETEELA TKVPDNGGVF FVPAFVGLGA PYWDMYARGL 370 380 390 400 410 420 IIGITRGTTR EHIVRAVLES IAYQTRDVVE VMEKDSDIKV ETLRVDGGAV VNNFLMQFQA 430 440 450 460 470 480 DILGVPVERP VVNETTALGA AYLAGLAVGY WKDQEEIASL WQLDRRFEPS MNSEERERLY 490 SKWKEAVSRS LGWEKR

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD36500.1. Sequence problems.
PIR	C72254.
RefSeq	NP_229230.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9X1E4.
SMR	Q9X1E4. Positions 2-495.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	898045.
GenomeReviews	Gene locus TM_1430 in contig AE000512_GR.
KEGG	tma:TM1430.
PATRIC	23937808. VBITheMar51294_1442.
TIGR	TM_1430.
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ALYGQLC.
PhylomeDB	Q9X1E4.
ProtClustDB	CLSK795900.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_1430-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK2_THEMA

Accession

Primary (citable) accession number: Q9X1E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program