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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (Tmari_0891)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (Tmari_1400)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (Tmari_1390), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (Tmari_0893)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Tmari_1450)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerUniRule annotation
Sitei21 – 211Transition state stabilizerUniRule annotation
Sitei149 – 1491Positions MEP for the nucleophilic attackUniRule annotation
Sitei201 – 2011Positions MEP for the nucleophilic attackUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:TM_1393
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2222222-C-methyl-D-erythritol 4-phosphate cytidylyltransferasePRO_0000075638Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1393.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi13 – 153Combined sources
Helixi21 – 233Combined sources
Helixi33 – 419Combined sources
Beta strandi46 – 527Combined sources
Helixi54 – 563Combined sources
Helixi57 – 615Combined sources
Beta strandi69 – 746Combined sources
Helixi79 – 9012Combined sources
Helixi91 – 933Combined sources
Beta strandi96 – 1027Combined sources
Helixi110 – 12314Combined sources
Beta strandi124 – 1318Combined sources
Beta strandi134 – 1407Combined sources
Beta strandi143 – 1475Combined sources
Beta strandi152 – 16211Combined sources
Helixi163 – 1708Combined sources
Beta strandi177 – 1793Combined sources
Helixi180 – 1845Combined sources
Turni185 – 1873Combined sources
Beta strandi191 – 1944Combined sources
Helixi197 – 1993Combined sources
Helixi207 – 21812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VPAX-ray2.67A/B1-222[»]
ProteinModelPortaliQ9X1B3.
SMRiQ9X1B3. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X1B3.

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
InParanoidiQ9X1B3.
KOiK00991.
OMAiPQVFDAD.
OrthoDBiEOG6B3662.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X1B3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVAILLAAG KGERMSENVP KQFLEIEGRM LFEYPLSTFL KSEAIDGVVI
60 70 80 90 100
VTRREWFEVV EKRVFHEKVL GIVEGGDTRS QSVRSALEFL EKFSPSYVLV
110 120 130 140 150
HDSARPFLRK KHVSEVLRRA RETGAATLAL KNSDALVRVE NDRIEYIPRK
160 170 180 190 200
GVYRILTPQA FSYEILKKAH ENGGEWADDT EPVQKLGVKI ALVEGDPLCF
210 220
KVTFKEDLEL ARIIAREWER IP
Length:222
Mass (Da):25,370
Last modified:November 1, 1999 - v1
Checksum:i51D5E945D078ACEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36464.1.
PIRiB72259.
RefSeqiNP_229194.1. NC_000853.1.
WP_004081602.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36464; AAD36464; TM_1393.
GeneIDi898083.
KEGGitma:TM1393.
PATRICi23937732. VBITheMar51294_1405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36464.1.
PIRiB72259.
RefSeqiNP_229194.1. NC_000853.1.
WP_004081602.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VPAX-ray2.67A/B1-222[»]
ProteinModelPortaliQ9X1B3.
SMRiQ9X1B3. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1393.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36464; AAD36464; TM_1393.
GeneIDi898083.
KEGGitma:TM1393.
PATRICi23937732. VBITheMar51294_1405.

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
InParanoidiQ9X1B3.
KOiK00991.
OMAiPQVFDAD.
OrthoDBiEOG6B3662.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.

Miscellaneous databases

EvolutionaryTraceiQ9X1B3.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiISPD_THEMA
AccessioniPrimary (citable) accession number: Q9X1B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.