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Q9X172

- SYE1_THEMA

UniProt

Q9X172 - SYE1_THEMA

Protein

Glutamate--tRNA ligase 1

Gene

gltX1

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei253 – 2531ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 1 (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase 1
    Short name:
    GluRS 1
    Gene namesi
    Name:gltX1
    Ordered Locus Names:TM_1351
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Glutamate--tRNA ligase 1PRO_0000119680Add
    BLAST

    Proteomic databases

    PRIDEiQ9X172.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi243274.TM1351.

    Structurei

    Secondary structure

    1
    469
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Helixi16 – 3217
    Beta strandi35 – 384
    Helixi48 – 503
    Helixi51 – 6313
    Beta strandi68 – 703
    Turni71 – 733
    Helixi82 – 854
    Helixi86 – 9813
    Beta strandi101 – 1055
    Turni110 – 1123
    Helixi113 – 1219
    Helixi130 – 1334
    Turni134 – 1363
    Helixi139 – 1479
    Beta strandi153 – 1564
    Beta strandi163 – 1675
    Turni168 – 1703
    Beta strandi171 – 1755
    Beta strandi182 – 1865
    Helixi194 – 20411
    Beta strandi208 – 2136
    Helixi214 – 2163
    Helixi217 – 22913
    Beta strandi236 – 2405
    Beta strandi248 – 2503
    Helixi253 – 2553
    Helixi260 – 2667
    Helixi270 – 2789
    Beta strandi280 – 2823
    Helixi293 – 2997
    Helixi302 – 3043
    Helixi314 – 32714
    Helixi330 – 34314
    Helixi351 – 36111
    Turni362 – 3643
    Helixi368 – 3703
    Helixi371 – 3744
    Helixi376 – 3783
    Helixi389 – 3913
    Helixi392 – 40110
    Turni402 – 4043
    Helixi409 – 42315
    Helixi427 – 43812
    Beta strandi440 – 4423
    Helixi447 – 4537
    Helixi456 – 46611

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2O5RX-ray2.34A1-469[»]
    ProteinModelPortaliQ9X172.
    SMRiQ9X172. Positions 1-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X172.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi8 – 1811"HIGH" regionAdd
    BLAST
    Motifi250 – 2545"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK09698.
    OMAiPEGMLNY.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    1.10.8.70. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X172-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRVRFAPSP TGFLHVGGAR TALFNFLFAR KEKGKFILRI EDTDLERSER    50
    EYEEKLMESL RWLGLLWDEG PDVGGDHGPY RQSERVEIYR EHAERLVKEG 100
    KAYYVYAYPE EIEEMREKLL SEGKAPHYSQ EMFEKFDTPE RRREYEEKGL 150
    RPAVFFKMPR KDYVLNDVVK GEVVFKTGAI GDFVIMRSNG LPTYNFACVV 200
    DDMLMEITHV IRGDDHLSNT LRQLALYEAF EKAPPVFAHV STILGPDGKK 250
    LSKRHGATSV EAFRDMGYLP EALVNYLALL GWSHPEGKEL LTLEELISSF 300
    SLDRLSPNPA IFDPQKLKWM NGYYLRNMPI EKLAELAKPF FEKAGIKIID 350
    EEYFKKVLEI TKERVEVLSE FPEESRFFFE DPAPVEIPEE MKEVFSQLKE 400
    ELQNVRWTME EITPVFKKVL KQHGVKPKEF YMTLRRVLTG REEGPELVNI 450
    IPLLGKEIFL RRIERSLGG 469
    Length:469
    Mass (Da):54,607
    Last modified:November 1, 1999 - v1
    Checksum:iF27D3AB4FE070BD8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36422.1.
    PIRiG72264.
    RefSeqiNP_229152.1. NC_000853.1.
    YP_007977707.1. NC_021214.1.
    YP_008991558.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36422; AAD36422; TM_1351.
    GeneIDi898129.
    KEGGitma:TM1351.
    tmi:THEMA_07585.
    tmm:Tmari_1358.
    PATRICi23937640. VBITheMar51294_1363.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36422.1 .
    PIRi G72264.
    RefSeqi NP_229152.1. NC_000853.1.
    YP_007977707.1. NC_021214.1.
    YP_008991558.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2O5R X-ray 2.34 A 1-469 [» ]
    ProteinModelPortali Q9X172.
    SMRi Q9X172. Positions 1-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1351.

    Proteomic databases

    PRIDEi Q9X172.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36422 ; AAD36422 ; TM_1351 .
    GeneIDi 898129.
    KEGGi tma:TM1351.
    tmi:THEMA_07585.
    tmm:Tmari_1358.
    PATRICi 23937640. VBITheMar51294_1363.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K09698.
    OMAi PEGMLNY.
    OrthoDBi EOG6DRPF7.

    Miscellaneous databases

    EvolutionaryTracei Q9X172.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    1.10.8.70. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Crystal structure of glutamyl-tRNA synthetase 1 (EC 6.1.1.17) (glutamate-tRNA ligase 1) (glurS 1) (TM1351) from Thermotoga maritima at 2.5 A resolution."
      Joint center for structural genomics (JCSG)
      Submitted (DEC-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS).

    Entry informationi

    Entry nameiSYE1_THEMA
    AccessioniPrimary (citable) accession number: Q9X172
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3