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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.By similarity

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi618 – 6181ZincBy similarity
Metal bindingi620 – 6201ZincBy similarity
Metal bindingi704 – 7041ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.1.1.14. 6331.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:metE
Ordered Locus Names:TM_1286
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7347345-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_0000098674Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1286.

Structurei

Secondary structure

1
734
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Helixi16 – 2510Combined sources
Helixi31 – 5222Combined sources
Beta strandi57 – 593Combined sources
Helixi66 – 738Combined sources
Helixi79 – 813Combined sources
Helixi87 – 948Combined sources
Beta strandi101 – 1044Combined sources
Beta strandi111 – 1144Combined sources
Helixi128 – 13710Combined sources
Turni138 – 1403Combined sources
Beta strandi144 – 1485Combined sources
Helixi150 – 1556Combined sources
Helixi167 – 19024Combined sources
Beta strandi196 – 1994Combined sources
Helixi201 – 2044Combined sources
Helixi209 – 22012Combined sources
Turni221 – 2244Combined sources
Beta strandi227 – 2304Combined sources
Helixi239 – 2435Combined sources
Beta strandi248 – 2536Combined sources
Helixi259 – 2679Combined sources
Beta strandi273 – 2808Combined sources
Helixi290 – 30011Combined sources
Beta strandi303 – 3097Combined sources
Helixi311 – 3133Combined sources
Beta strandi323 – 3253Combined sources
Turni326 – 3283Combined sources
Helixi329 – 3313Combined sources
Helixi335 – 34915Combined sources
Turni362 – 3654Combined sources
Helixi368 – 3758Combined sources
Helixi379 – 3813Combined sources
Helixi388 – 39912Combined sources
Helixi416 – 42611Combined sources
Helixi432 – 45322Combined sources
Beta strandi456 – 4583Combined sources
Helixi468 – 4736Combined sources
Beta strandi476 – 4805Combined sources
Beta strandi487 – 4904Combined sources
Beta strandi493 – 4953Combined sources
Beta strandi499 – 5057Combined sources
Helixi512 – 5209Combined sources
Beta strandi526 – 5316Combined sources
Helixi533 – 5386Combined sources
Beta strandi540 – 5423Combined sources
Beta strandi544 – 5463Combined sources
Helixi548 – 56821Combined sources
Beta strandi573 – 5775Combined sources
Helixi580 – 5834Combined sources
Helixi589 – 5913Combined sources
Helixi592 – 60615Combined sources
Beta strandi613 – 6186Combined sources
Turni625 – 6273Combined sources
Helixi628 – 6314Combined sources
Beta strandi637 – 6426Combined sources
Turni644 – 6485Combined sources
Helixi649 – 6513Combined sources
Helixi652 – 6554Combined sources
Beta strandi662 – 6676Combined sources
Beta strandi673 – 6753Combined sources
Helixi679 – 68911Combined sources
Turni690 – 6923Combined sources
Helixi695 – 6973Combined sources
Beta strandi698 – 7014Combined sources
Helixi711 – 73121Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T7LX-ray2.00A/B2-734[»]
1XDJX-ray2.20A/B2-734[»]
1XPGX-ray2.59A/B2-732[»]
1XR2X-ray2.35A/B2-734[»]
3BQ5X-ray2.00A/B2-734[»]
3BQ6X-ray2.10A/B2-734[»]
ProteinModelPortaliQ9X112.
SMRiQ9X112. Positions 2-733.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X112.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DSS. Bacteria.
COG0620. LUCA.
InParanoidiQ9X112.
KOiK00549.
OMAiRFGWVQS.
OrthoDBiEOG6FFS3G.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYAFGFPK IGEKREFKKA LEDFWKGKIT EEQFEEEMNK LRMYMVENYR
60 70 80 90 100
KNVDVIPSNE LSYYDFVLDT AVMVGAVPER FGEYRGLSTY FDMARGGKAL
110 120 130 140 150
EMTKFFNTNY HYLVPEIETE EFYLLENKPL EDYLFFKSKG IETAPWVIGP
160 170 180 190 200
FTFLYLSKRN GEWIRRPNQM EKLLESLVSV YKEVFEKLVE NGCKEILVNE
210 220 230 240 250
PAFVCDLEKA HWDLILNVYR ELSEFPLTVF TYYDSVSDYE ACVSLPVKRL
260 270 280 290 300
HFDFVSNEEN LKNLEKHGFP EDKKLVAGVI NGRQPWKVDL RKVASLVEKL
310 320 330 340 350
GASAISNSCP LFHLPVTLEL ENNLPGGLKE KLAFAKEKLE ELKMLKDFLE
360 370 380 390 400
GKTFDLPNVS FEDFAVDLQA VERVRNLPED SFRREKEYTE RDRIQRERLN
410 420 430 440 450
LPLFPTTTIG SFPQTPEVRK MRSKYRKGEI SKEEYEAFIK EQIKKAIELQ
460 470 480 490 500
EEIGLDVLVH GEFERTDMVE FFAEKLNGIA TTQNGWVLSY GSRCYRPPII
510 520 530 540 550
YGTVTRPEPM TLKEITYAQS LTEKPVKGML TGPVTIMSWS YYREDIPERE
560 570 580 590 600
IAYQIALAIN EEVKDLEEAG IKIVQIDEPA FREKAPIKKS KWPEYFEWAI
610 620 630 640 650
NAFNLAANAR PETQIHAHMC YSDFNEIIEY IHQLEFDVIS IEASRSKGEI
660 670 680 690 700
ISAFENFKGW IKQIGVGVWD IHSPAVPSIN EMREIVERVL RVLPKELIWI
710 720 730
NPDCGLKTRN WDEVIPSLRN MVALAKEMRE KFES
Length:734
Mass (Da):85,571
Last modified:November 1, 1999 - v1
Checksum:i34B19187480D443B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36360.1.
PIRiE72271.
RefSeqiNP_229090.1. NC_000853.1.
WP_004079949.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36360; AAD36360; TM_1286.
GeneIDi898197.
KEGGitma:TM1286.
PATRICi23937512. VBITheMar51294_1302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36360.1.
PIRiE72271.
RefSeqiNP_229090.1. NC_000853.1.
WP_004079949.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T7LX-ray2.00A/B2-734[»]
1XDJX-ray2.20A/B2-734[»]
1XPGX-ray2.59A/B2-732[»]
1XR2X-ray2.35A/B2-734[»]
3BQ5X-ray2.00A/B2-734[»]
3BQ6X-ray2.10A/B2-734[»]
ProteinModelPortaliQ9X112.
SMRiQ9X112. Positions 2-733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1286.

Protocols and materials databases

DNASUi898197.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36360; AAD36360; TM_1286.
GeneIDi898197.
KEGGitma:TM1286.
PATRICi23937512. VBITheMar51294_1302.

Phylogenomic databases

eggNOGiENOG4105DSS. Bacteria.
COG0620. LUCA.
InParanoidiQ9X112.
KOiK00549.
OMAiRFGWVQS.
OrthoDBiEOG6FFS3G.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BRENDAi2.1.1.14. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X112.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiMETE_THEMA
AccessioniPrimary (citable) accession number: Q9X112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.