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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.By similarity

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi618ZincBy similarity1
Metal bindingi620ZincBy similarity1
Metal bindingi704ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.1.1.14. 6331.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:metE
Ordered Locus Names:TM_1286
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000986741 – 7345-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST734

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1286.

Structurei

Secondary structure

1734
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Helixi16 – 25Combined sources10
Helixi31 – 52Combined sources22
Beta strandi57 – 59Combined sources3
Helixi66 – 73Combined sources8
Helixi79 – 81Combined sources3
Helixi87 – 94Combined sources8
Beta strandi101 – 104Combined sources4
Beta strandi111 – 114Combined sources4
Helixi128 – 137Combined sources10
Turni138 – 140Combined sources3
Beta strandi144 – 148Combined sources5
Helixi150 – 155Combined sources6
Helixi167 – 190Combined sources24
Beta strandi196 – 199Combined sources4
Helixi201 – 204Combined sources4
Helixi209 – 220Combined sources12
Turni221 – 224Combined sources4
Beta strandi227 – 230Combined sources4
Helixi239 – 243Combined sources5
Beta strandi248 – 253Combined sources6
Helixi259 – 267Combined sources9
Beta strandi273 – 280Combined sources8
Helixi290 – 300Combined sources11
Beta strandi303 – 309Combined sources7
Helixi311 – 313Combined sources3
Beta strandi323 – 325Combined sources3
Turni326 – 328Combined sources3
Helixi329 – 331Combined sources3
Helixi335 – 349Combined sources15
Turni362 – 365Combined sources4
Helixi368 – 375Combined sources8
Helixi379 – 381Combined sources3
Helixi388 – 399Combined sources12
Helixi416 – 426Combined sources11
Helixi432 – 453Combined sources22
Beta strandi456 – 458Combined sources3
Helixi468 – 473Combined sources6
Beta strandi476 – 480Combined sources5
Beta strandi487 – 490Combined sources4
Beta strandi493 – 495Combined sources3
Beta strandi499 – 505Combined sources7
Helixi512 – 520Combined sources9
Beta strandi526 – 531Combined sources6
Helixi533 – 538Combined sources6
Beta strandi540 – 542Combined sources3
Beta strandi544 – 546Combined sources3
Helixi548 – 568Combined sources21
Beta strandi573 – 577Combined sources5
Helixi580 – 583Combined sources4
Helixi589 – 591Combined sources3
Helixi592 – 606Combined sources15
Beta strandi613 – 618Combined sources6
Turni625 – 627Combined sources3
Helixi628 – 631Combined sources4
Beta strandi637 – 642Combined sources6
Turni644 – 648Combined sources5
Helixi649 – 651Combined sources3
Helixi652 – 655Combined sources4
Beta strandi662 – 667Combined sources6
Beta strandi673 – 675Combined sources3
Helixi679 – 689Combined sources11
Turni690 – 692Combined sources3
Helixi695 – 697Combined sources3
Beta strandi698 – 701Combined sources4
Helixi711 – 731Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T7LX-ray2.00A/B2-734[»]
1XDJX-ray2.20A/B2-734[»]
1XPGX-ray2.59A/B2-732[»]
1XR2X-ray2.35A/B2-734[»]
3BQ5X-ray2.00A/B2-734[»]
3BQ6X-ray2.10A/B2-734[»]
ProteinModelPortaliQ9X112.
SMRiQ9X112.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X112.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DSS. Bacteria.
COG0620. LUCA.
InParanoidiQ9X112.
KOiK00549.
OMAiRFGWVQS.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYAFGFPK IGEKREFKKA LEDFWKGKIT EEQFEEEMNK LRMYMVENYR
60 70 80 90 100
KNVDVIPSNE LSYYDFVLDT AVMVGAVPER FGEYRGLSTY FDMARGGKAL
110 120 130 140 150
EMTKFFNTNY HYLVPEIETE EFYLLENKPL EDYLFFKSKG IETAPWVIGP
160 170 180 190 200
FTFLYLSKRN GEWIRRPNQM EKLLESLVSV YKEVFEKLVE NGCKEILVNE
210 220 230 240 250
PAFVCDLEKA HWDLILNVYR ELSEFPLTVF TYYDSVSDYE ACVSLPVKRL
260 270 280 290 300
HFDFVSNEEN LKNLEKHGFP EDKKLVAGVI NGRQPWKVDL RKVASLVEKL
310 320 330 340 350
GASAISNSCP LFHLPVTLEL ENNLPGGLKE KLAFAKEKLE ELKMLKDFLE
360 370 380 390 400
GKTFDLPNVS FEDFAVDLQA VERVRNLPED SFRREKEYTE RDRIQRERLN
410 420 430 440 450
LPLFPTTTIG SFPQTPEVRK MRSKYRKGEI SKEEYEAFIK EQIKKAIELQ
460 470 480 490 500
EEIGLDVLVH GEFERTDMVE FFAEKLNGIA TTQNGWVLSY GSRCYRPPII
510 520 530 540 550
YGTVTRPEPM TLKEITYAQS LTEKPVKGML TGPVTIMSWS YYREDIPERE
560 570 580 590 600
IAYQIALAIN EEVKDLEEAG IKIVQIDEPA FREKAPIKKS KWPEYFEWAI
610 620 630 640 650
NAFNLAANAR PETQIHAHMC YSDFNEIIEY IHQLEFDVIS IEASRSKGEI
660 670 680 690 700
ISAFENFKGW IKQIGVGVWD IHSPAVPSIN EMREIVERVL RVLPKELIWI
710 720 730
NPDCGLKTRN WDEVIPSLRN MVALAKEMRE KFES
Length:734
Mass (Da):85,571
Last modified:November 1, 1999 - v1
Checksum:i34B19187480D443B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36360.1.
PIRiE72271.
RefSeqiNP_229090.1. NC_000853.1.
WP_004079949.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36360; AAD36360; TM_1286.
GeneIDi898197.
KEGGitma:TM1286.
PATRICi23937512. VBITheMar51294_1302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36360.1.
PIRiE72271.
RefSeqiNP_229090.1. NC_000853.1.
WP_004079949.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T7LX-ray2.00A/B2-734[»]
1XDJX-ray2.20A/B2-734[»]
1XPGX-ray2.59A/B2-732[»]
1XR2X-ray2.35A/B2-734[»]
3BQ5X-ray2.00A/B2-734[»]
3BQ6X-ray2.10A/B2-734[»]
ProteinModelPortaliQ9X112.
SMRiQ9X112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1286.

Protocols and materials databases

DNASUi898197.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36360; AAD36360; TM_1286.
GeneIDi898197.
KEGGitma:TM1286.
PATRICi23937512. VBITheMar51294_1302.

Phylogenomic databases

eggNOGiENOG4105DSS. Bacteria.
COG0620. LUCA.
InParanoidiQ9X112.
KOiK00549.
OMAiRFGWVQS.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BRENDAi2.1.1.14. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X112.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETE_THEMA
AccessioniPrimary (citable) accession number: Q9X112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.