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Q9X108

- BGLT_THEMA

UniProt

Q9X108 - BGLT_THEMA

Protein

6-phospho-beta-glucosidase BglT

Gene

bglT

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Hydrolyzes cellobiose 6'-phosphate into glucose 6-phosphate (Glc6P) and glucose.

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Cofactori

    Binds 1 NAD per subunit.
    Binds 1 manganese ion per subunit.

    Kineticsi

    1. KM=41 µM for p-nitrophenyl-beta-D-glucopyranoside 6-phosphate

    pH dependencei

    Optimum pH is 8.0. Active from pH 6.5 to 10.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871Substrate
    Sitei103 – 1031Increases basicity of active site TyrBy similarity
    Binding sitei140 – 1401Substrate
    Metal bindingi162 – 1621Manganese
    Binding sitei163 – 1631Substrate
    Metal bindingi192 – 1921Manganese
    Active sitei241 – 2411Proton acceptor
    Binding sitei261 – 2611Substrate

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1 – 6464NADAdd
    BLAST

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    SABIO-RKQ9X108.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-glucosidase BglT (EC:3.2.1.86)
    Gene namesi
    Name:bglT
    Ordered Locus Names:TM_1281
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4154156-phospho-beta-glucosidase BglTPRO_0000169857Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer. Exists in a homodimer/homotetramer equilibrium state in solution.

    Protein-protein interaction databases

    STRINGi243274.TM1281.

    Structurei

    Secondary structure

    1
    415
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76
    Helixi13 – 2311
    Turni24 – 263
    Beta strandi31 – 355
    Helixi39 – 5315
    Beta strandi56 – 616
    Helixi65 – 695
    Beta strandi73 – 775
    Helixi83 – 9210
    Helixi93 – 975
    Beta strandi103 – 1053
    Helixi107 – 12923
    Beta strandi134 – 1374
    Beta strandi139 – 1413
    Helixi142 – 15110
    Beta strandi156 – 1605
    Helixi164 – 17512
    Helixi180 – 1823
    Beta strandi183 – 1908
    Beta strandi193 – 2019
    Helixi207 – 2148
    Helixi228 – 2347
    Beta strandi236 – 2383
    Helixi240 – 2423
    Helixi243 – 2464
    Helixi248 – 2569
    Helixi261 – 27616
    Helixi284 – 2885
    Turni290 – 2934
    Helixi294 – 30613
    Beta strandi307 – 3093
    Beta strandi311 – 3188
    Beta strandi331 – 3399
    Beta strandi342 – 3465
    Helixi353 – 37422
    Helixi378 – 38710
    Helixi394 – 40714
    Turni408 – 4114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UP4X-ray2.85A/B/C/D/E/F/G/H1-415[»]
    1UP6X-ray2.55A/B/C/D/E/F/G/H1-415[»]
    1UP7X-ray2.40A/B/C/D/E/F/G/H1-415[»]
    ProteinModelPortaliQ9X108.
    SMRiQ9X108. Positions 1-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X108.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Phylogenomic databases

    eggNOGiCOG1486.
    KOiK01222.
    OMAiPSGHITE.
    OrthoDBiEOG6CP3SG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X108-1 [UniParc]FASTAAdd to Basket

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    MRIAVIGGGS SYTPELVKGL LDISEDVRID EVIFYDIDEE KQKIVVDFVK    50
    RLVKDRFKVL ISDTFEGAVV DAKYVIFQFR PGGLKGREND EGIPLKYGLI 100
    GQETTGVGGF SAALRAFPIV EEYVDTVRKT SNATIVNFTN PSGHITEFVR 150
    NYLEYEKFIG LCNVPINFIR EIAEMFSARL EDVFLKYYGL NHLSFIEKVF 200
    VKGEDVTEKV FENLKLKLSN IPDEDFPTWF YDSVRLIVNP YLRYYLMEKK 250
    MFKKISTHEL RAREVMKIEK ELFEKYRTAV EIPEELTKRG GSMYSTAAAH 300
    LIRDLETDEG KIHIVNTRNN GSIENLPDDY VLEIPCYVRS GRVHTLSQGK 350
    GDHFALSFIH AVKMYERLTI EAYLKRSKKL ALKALLSHPL GPDVEDAKDL 400
    LEEILEANRE YVKLG 415
    Length:415
    Mass (Da):47,627
    Last modified:November 1, 1999 - v1
    Checksum:iFB4E3B358245BFEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36356.1.
    PIRiE72273.
    RefSeqiNP_229086.1. NC_000853.1.
    WP_004079953.1. NC_023151.1.
    YP_007977635.1. NC_021214.1.
    YP_008991622.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36356; AAD36356; TM_1281.
    GeneIDi898202.
    KEGGitma:TM1281.
    tmi:THEMA_07930.
    tmm:Tmari_1286.
    PATRICi23937500. VBITheMar51294_1296.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36356.1 .
    PIRi E72273.
    RefSeqi NP_229086.1. NC_000853.1.
    WP_004079953.1. NC_023151.1.
    YP_007977635.1. NC_021214.1.
    YP_008991622.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UP4 X-ray 2.85 A/B/C/D/E/F/G/H 1-415 [» ]
    1UP6 X-ray 2.55 A/B/C/D/E/F/G/H 1-415 [» ]
    1UP7 X-ray 2.40 A/B/C/D/E/F/G/H 1-415 [» ]
    ProteinModelPortali Q9X108.
    SMRi Q9X108. Positions 1-415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1281.

    Protein family/group databases

    CAZyi GH4. Glycoside Hydrolase Family 4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36356 ; AAD36356 ; TM_1281 .
    GeneIDi 898202.
    KEGGi tma:TM1281.
    tmi:THEMA_07930.
    tmm:Tmari_1286.
    PATRICi 23937500. VBITheMar51294_1296.

    Phylogenomic databases

    eggNOGi COG1486.
    KOi K01222.
    OMAi PSGHITE.
    OrthoDBi EOG6CP3SG.

    Enzyme and pathway databases

    SABIO-RK Q9X108.

    Miscellaneous databases

    EvolutionaryTracei Q9X108.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view ]
    PRINTSi PR00732. GLHYDRLASE4.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima."
      Yip V.L.Y., Varrot A., Davies G.J., Rajan S.S., Yang X., Thompson J., Anderson W.F., Withers S.G.
      J. Am. Chem. Soc. 126:8354-8355(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, REACTION MECHANISM.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "NAD(+) and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides."
      Varrot A., Yip V.L.Y., Li Y., Rajan S.S., Yang X., Anderson W.F., Thompson J., Withers S.G., Davies G.J.
      J. Mol. Biol. 346:423-435(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF NATIVE PROTEIN AND COMPLEX WITH NAD(+) AND GLUCOSE-6-PHOSPHATE.

    Entry informationi

    Entry nameiBGLT_THEMA
    AccessioniPrimary (citable) accession number: Q9X108
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Is a retaining glucosidase as it hydrolyzes glycosidic bond with retention of anomeric configuration. Reaction mechanism includes redox steps involving NAD and stabilization of intermediates by Mn2+.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3