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Protein

6-phospho-beta-glucosidase BglT

Gene

bglT

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes cellobiose 6'-phosphate into glucose 6-phosphate (Glc6P) and glucose.

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • NAD+Note: Binds 1 NAD+ per subunit.
  • Mn2+Note: Binds 1 Mn2+ ion per subunit.

Kineticsi

  1. KM=41 µM for p-nitrophenyl-beta-D-glucopyranoside 6-phosphate

    pH dependencei

    Optimum pH is 8.0. Active from pH 6.5 to 10.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei87Substrate1
    Sitei103Increases basicity of active site TyrBy similarity1
    Binding sitei140Substrate1
    Metal bindingi162Manganese1
    Binding sitei163Substrate1
    Metal bindingi192Manganese1
    Active sitei241Proton acceptor1
    Binding sitei261Substrate1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi1 – 64NADAdd BLAST64

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    BRENDAi3.2.1.86. 6331.
    SABIO-RKQ9X108.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-glucosidase BglT (EC:3.2.1.86)
    Gene namesi
    Name:bglT
    Ordered Locus Names:TM_1281
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    Proteomesi
    • UP000008183 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001698571 – 4156-phospho-beta-glucosidase BglTAdd BLAST415

    Interactioni

    Subunit structurei

    Homodimer or homotetramer. Exists in a homodimer/homotetramer equilibrium state in solution.

    Protein-protein interaction databases

    STRINGi243274.TM1281.

    Structurei

    Secondary structure

    1415
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 7Combined sources6
    Helixi13 – 23Combined sources11
    Turni24 – 26Combined sources3
    Beta strandi31 – 35Combined sources5
    Helixi39 – 53Combined sources15
    Beta strandi56 – 61Combined sources6
    Helixi65 – 69Combined sources5
    Beta strandi73 – 77Combined sources5
    Helixi83 – 92Combined sources10
    Helixi93 – 97Combined sources5
    Beta strandi103 – 105Combined sources3
    Helixi107 – 129Combined sources23
    Beta strandi134 – 137Combined sources4
    Beta strandi139 – 141Combined sources3
    Helixi142 – 151Combined sources10
    Beta strandi156 – 160Combined sources5
    Helixi164 – 175Combined sources12
    Helixi180 – 182Combined sources3
    Beta strandi183 – 190Combined sources8
    Beta strandi193 – 201Combined sources9
    Helixi207 – 214Combined sources8
    Helixi228 – 234Combined sources7
    Beta strandi236 – 238Combined sources3
    Helixi240 – 242Combined sources3
    Helixi243 – 246Combined sources4
    Helixi248 – 256Combined sources9
    Helixi261 – 276Combined sources16
    Helixi284 – 288Combined sources5
    Turni290 – 293Combined sources4
    Helixi294 – 306Combined sources13
    Beta strandi307 – 309Combined sources3
    Beta strandi311 – 318Combined sources8
    Beta strandi331 – 339Combined sources9
    Beta strandi342 – 346Combined sources5
    Helixi353 – 374Combined sources22
    Helixi378 – 387Combined sources10
    Helixi394 – 407Combined sources14
    Turni408 – 411Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1UP4X-ray2.85A/B/C/D/E/F/G/H1-415[»]
    1UP6X-ray2.55A/B/C/D/E/F/G/H1-415[»]
    1UP7X-ray2.40A/B/C/D/E/F/G/H1-415[»]
    ProteinModelPortaliQ9X108.
    SMRiQ9X108.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X108.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105F8D. Bacteria.
    COG1486. LUCA.
    InParanoidiQ9X108.
    KOiK01222.
    OMAiCNVPINF.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X108-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRIAVIGGGS SYTPELVKGL LDISEDVRID EVIFYDIDEE KQKIVVDFVK
    60 70 80 90 100
    RLVKDRFKVL ISDTFEGAVV DAKYVIFQFR PGGLKGREND EGIPLKYGLI
    110 120 130 140 150
    GQETTGVGGF SAALRAFPIV EEYVDTVRKT SNATIVNFTN PSGHITEFVR
    160 170 180 190 200
    NYLEYEKFIG LCNVPINFIR EIAEMFSARL EDVFLKYYGL NHLSFIEKVF
    210 220 230 240 250
    VKGEDVTEKV FENLKLKLSN IPDEDFPTWF YDSVRLIVNP YLRYYLMEKK
    260 270 280 290 300
    MFKKISTHEL RAREVMKIEK ELFEKYRTAV EIPEELTKRG GSMYSTAAAH
    310 320 330 340 350
    LIRDLETDEG KIHIVNTRNN GSIENLPDDY VLEIPCYVRS GRVHTLSQGK
    360 370 380 390 400
    GDHFALSFIH AVKMYERLTI EAYLKRSKKL ALKALLSHPL GPDVEDAKDL
    410
    LEEILEANRE YVKLG
    Length:415
    Mass (Da):47,627
    Last modified:November 1, 1999 - v1
    Checksum:iFB4E3B358245BFEE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36356.1.
    PIRiE72273.
    RefSeqiNP_229086.1. NC_000853.1.
    WP_004079953.1. NZ_CP011107.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36356; AAD36356; TM_1281.
    GeneIDi898202.
    KEGGitma:TM1281.
    PATRICi23937500. VBITheMar51294_1296.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36356.1.
    PIRiE72273.
    RefSeqiNP_229086.1. NC_000853.1.
    WP_004079953.1. NZ_CP011107.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1UP4X-ray2.85A/B/C/D/E/F/G/H1-415[»]
    1UP6X-ray2.55A/B/C/D/E/F/G/H1-415[»]
    1UP7X-ray2.40A/B/C/D/E/F/G/H1-415[»]
    ProteinModelPortaliQ9X108.
    SMRiQ9X108.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi243274.TM1281.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD36356; AAD36356; TM_1281.
    GeneIDi898202.
    KEGGitma:TM1281.
    PATRICi23937500. VBITheMar51294_1296.

    Phylogenomic databases

    eggNOGiENOG4105F8D. Bacteria.
    COG1486. LUCA.
    InParanoidiQ9X108.
    KOiK01222.
    OMAiCNVPINF.

    Enzyme and pathway databases

    BRENDAi3.2.1.86. 6331.
    SABIO-RKQ9X108.

    Miscellaneous databases

    EvolutionaryTraceiQ9X108.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBGLT_THEMA
    AccessioniPrimary (citable) accession number: Q9X108
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: November 1, 1999
    Last modified: November 2, 2016
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Is a retaining glucosidase as it hydrolyzes glycosidic bond with retention of anomeric configuration. Reaction mechanism includes redox steps involving NAD and stabilization of intermediates by Mn2+.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.