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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:TM_1273
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000148857Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

DIPiDIP-59229N.
STRINGi243274.TM1273.

Structurei

Secondary structure

1
482
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147Combined sources
Beta strandi21 – 277Combined sources
Turni40 – 445Combined sources
Helixi54 – 6613Combined sources
Beta strandi73 – 753Combined sources
Beta strandi78 – 814Combined sources
Beta strandi90 – 945Combined sources
Beta strandi96 – 983Combined sources
Beta strandi104 – 1085Combined sources
Beta strandi111 – 12515Combined sources
Beta strandi130 – 1334Combined sources
Beta strandi136 – 1394Combined sources
Beta strandi142 – 1476Combined sources
Helixi148 – 1503Combined sources
Beta strandi155 – 1606Combined sources
Helixi167 – 18418Combined sources
Helixi191 – 1933Combined sources
Beta strandi195 – 2017Combined sources
Beta strandi206 – 2083Combined sources
Helixi225 – 23612Combined sources
Beta strandi239 – 2457Combined sources
Beta strandi254 – 2563Combined sources
Turni257 – 2604Combined sources
Beta strandi261 – 2633Combined sources
Helixi289 – 2924Combined sources
Beta strandi295 – 2995Combined sources
Helixi305 – 31410Combined sources
Helixi318 – 3247Combined sources
Helixi328 – 34013Combined sources
Helixi344 – 3529Combined sources
Helixi354 – 3585Combined sources
Turni359 – 3635Combined sources
Beta strandi368 – 3703Combined sources
Helixi373 – 38311Combined sources
Turni384 – 3863Combined sources
Helixi392 – 3954Combined sources
Helixi397 – 4037Combined sources
Helixi407 – 4148Combined sources
Helixi425 – 43410Combined sources
Helixi437 – 4437Combined sources
Helixi452 – 4609Combined sources
Helixi468 – 47811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AL0X-ray3.37B1-482[»]
ProteinModelPortaliQ9X100.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X100.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
InParanoidiQ9X100.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYRPVIGLE IHVQLSTKTK AFCSCPADVF ELPPNTAICP VCTGQPGALP
60 70 80 90 100
VPNEEMIRFA VKTALALNCK IHKYSRFDRK NYFYPDLPKG YQISQYFYPI
110 120 130 140 150
ATEGFLEIDG DEGRKKVRIR RLHLEEDAGK LVHEGDSITR ASYSLVDMNR
160 170 180 190 200
CGVPLIEIVT EPDISSPREA RVFMEKLRSI VRYLGVSTGD MEKGALRCDA
210 220 230 240 250
NISVVDTETG RQSNRVEVKN MNSFRFVERA LEYEFERIVK AMERGEDVER
260 270 280 290 300
ETRGWDMATK ITVSMRGKEE ESDYRYFPEP DIPPVVLSDE YLEEVKKELP
310 320 330 340 350
ELPDEKAERF MREYGLPEYD AKVLTSSKEL AEFFEECVKV VNRPKDLSNW
360 370 380 390 400
IMTEVLRELN ERNIEITESK LTPQHFADLF KLMDEGKISI KIAKEIFPEV
410 420 430 440 450
FETGKMPSQI VEEKGLTQIN DEKLIEELVK KAMEQNPKAV QDYKSGKKKA
460 470 480
AGFFVGYVMR ETKGKANPEL TNRIIQKLLE GE
Length:482
Mass (Da):55,436
Last modified:November 1, 1999 - v1
Checksum:i34988170E32F312F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36348.1.
PIRiH72274.
RefSeqiNP_229078.1. NC_000853.1.
WP_004079967.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36348; AAD36348; TM_1273.
GeneIDi898210.
KEGGitma:TM1273.
PATRICi23937484. VBITheMar51294_1288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36348.1.
PIRiH72274.
RefSeqiNP_229078.1. NC_000853.1.
WP_004079967.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AL0X-ray3.37B1-482[»]
ProteinModelPortaliQ9X100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59229N.
STRINGi243274.TM1273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36348; AAD36348; TM_1273.
GeneIDi898210.
KEGGitma:TM1273.
PATRICi23937484. VBITheMar51294_1288.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
InParanoidiQ9X100.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Miscellaneous databases

EvolutionaryTraceiQ9X100.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiGATB_THEMA
AccessioniPrimary (citable) accession number: Q9X100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.