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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:TM_1273
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001488571 – 482Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST482

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

DIPiDIP-59229N.
STRINGi243274.TM1273.

Structurei

Secondary structure

1482
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Beta strandi21 – 27Combined sources7
Turni40 – 44Combined sources5
Helixi54 – 66Combined sources13
Beta strandi73 – 75Combined sources3
Beta strandi78 – 81Combined sources4
Beta strandi90 – 94Combined sources5
Beta strandi96 – 98Combined sources3
Beta strandi104 – 108Combined sources5
Beta strandi111 – 125Combined sources15
Beta strandi130 – 133Combined sources4
Beta strandi136 – 139Combined sources4
Beta strandi142 – 147Combined sources6
Helixi148 – 150Combined sources3
Beta strandi155 – 160Combined sources6
Helixi167 – 184Combined sources18
Helixi191 – 193Combined sources3
Beta strandi195 – 201Combined sources7
Beta strandi206 – 208Combined sources3
Helixi225 – 236Combined sources12
Beta strandi239 – 245Combined sources7
Beta strandi254 – 256Combined sources3
Turni257 – 260Combined sources4
Beta strandi261 – 263Combined sources3
Helixi289 – 292Combined sources4
Beta strandi295 – 299Combined sources5
Helixi305 – 314Combined sources10
Helixi318 – 324Combined sources7
Helixi328 – 340Combined sources13
Helixi344 – 352Combined sources9
Helixi354 – 358Combined sources5
Turni359 – 363Combined sources5
Beta strandi368 – 370Combined sources3
Helixi373 – 383Combined sources11
Turni384 – 386Combined sources3
Helixi392 – 395Combined sources4
Helixi397 – 403Combined sources7
Helixi407 – 414Combined sources8
Helixi425 – 434Combined sources10
Helixi437 – 443Combined sources7
Helixi452 – 460Combined sources9
Helixi468 – 478Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AL0X-ray3.37B1-482[»]
ProteinModelPortaliQ9X100.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X100.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
InParanoidiQ9X100.
KOiK02434.
OMAiESADYRY.

Family and domain databases

HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYRPVIGLE IHVQLSTKTK AFCSCPADVF ELPPNTAICP VCTGQPGALP
60 70 80 90 100
VPNEEMIRFA VKTALALNCK IHKYSRFDRK NYFYPDLPKG YQISQYFYPI
110 120 130 140 150
ATEGFLEIDG DEGRKKVRIR RLHLEEDAGK LVHEGDSITR ASYSLVDMNR
160 170 180 190 200
CGVPLIEIVT EPDISSPREA RVFMEKLRSI VRYLGVSTGD MEKGALRCDA
210 220 230 240 250
NISVVDTETG RQSNRVEVKN MNSFRFVERA LEYEFERIVK AMERGEDVER
260 270 280 290 300
ETRGWDMATK ITVSMRGKEE ESDYRYFPEP DIPPVVLSDE YLEEVKKELP
310 320 330 340 350
ELPDEKAERF MREYGLPEYD AKVLTSSKEL AEFFEECVKV VNRPKDLSNW
360 370 380 390 400
IMTEVLRELN ERNIEITESK LTPQHFADLF KLMDEGKISI KIAKEIFPEV
410 420 430 440 450
FETGKMPSQI VEEKGLTQIN DEKLIEELVK KAMEQNPKAV QDYKSGKKKA
460 470 480
AGFFVGYVMR ETKGKANPEL TNRIIQKLLE GE
Length:482
Mass (Da):55,436
Last modified:November 1, 1999 - v1
Checksum:i34988170E32F312F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36348.1.
PIRiH72274.
RefSeqiNP_229078.1. NC_000853.1.
WP_004079967.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36348; AAD36348; TM_1273.
GeneIDi898210.
KEGGitma:TM1273.
PATRICi23937484. VBITheMar51294_1288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36348.1.
PIRiH72274.
RefSeqiNP_229078.1. NC_000853.1.
WP_004079967.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AL0X-ray3.37B1-482[»]
ProteinModelPortaliQ9X100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59229N.
STRINGi243274.TM1273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36348; AAD36348; TM_1273.
GeneIDi898210.
KEGGitma:TM1273.
PATRICi23937484. VBITheMar51294_1288.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
InParanoidiQ9X100.
KOiK02434.
OMAiESADYRY.

Miscellaneous databases

EvolutionaryTraceiQ9X100.

Family and domain databases

HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATB_THEMA
AccessioniPrimary (citable) accession number: Q9X100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: October 5, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.