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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay systemBy similarity
Active sitei141 – 1411Charge relay systemBy similarity
Active sitei165 – 1651Acyl-ester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1303-MONOMER.
BRENDAi6.3.5.7. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A (EC:6.3.5.7)
Short name:
Glu-ADT subunit A
Gene namesi
Name:gatA
Ordered Locus Names:TM_1272
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000105221Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

DIPiDIP-59228N.
STRINGi243274.TM1272.

Structurei

Secondary structure

1
475
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 83Combined sources
Helixi11 – 144Combined sources
Helixi19 – 224Combined sources
Helixi25 – 4016Combined sources
Beta strandi43 – 464Combined sources
Turni57 – 604Combined sources
Beta strandi62 – 665Combined sources
Beta strandi72 – 743Combined sources
Helixi81 – 833Combined sources
Helixi93 – 1019Combined sources
Beta strandi104 – 1096Combined sources
Helixi113 – 1153Combined sources
Beta strandi118 – 1203Combined sources
Beta strandi140 – 1423Combined sources
Helixi143 – 1508Combined sources
Beta strandi153 – 16412Combined sources
Helixi167 – 1737Combined sources
Beta strandi175 – 1795Combined sources
Turni195 – 1973Combined sources
Beta strandi199 – 2068Combined sources
Helixi207 – 21711Combined sources
Turni236 – 2394Combined sources
Beta strandi246 – 2505Combined sources
Helixi251 – 2555Combined sources
Helixi260 – 27516Combined sources
Beta strandi279 – 2835Combined sources
Helixi288 – 2903Combined sources
Helixi291 – 30414Combined sources
Beta strandi311 – 3166Combined sources
Helixi324 – 33512Combined sources
Helixi336 – 38045Combined sources
Beta strandi381 – 3888Combined sources
Beta strandi390 – 3923Combined sources
Turni396 – 3983Combined sources
Helixi402 – 4065Combined sources
Turni407 – 4126Combined sources
Helixi413 – 4186Combined sources
Beta strandi422 – 4309Combined sources
Beta strandi433 – 4408Combined sources
Helixi446 – 45914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GI3X-ray1.80A1-475[»]
3AL0X-ray3.37A1-475[»]
ProteinModelPortaliQ9X0Z9.
SMRiQ9X0Z9. Positions 1-475.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X0Z9.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
InParanoidiQ9X0Z9.
KOiK02433.
OMAiSKQYRER.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X0Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDLDFRKLT IEECLKLSEE EREKLPQLSL ETIKRLDPHV KAFISVRENV
60 70 80 90 100
SVEKKGKFWG IPVAIKDNIL TLGMRTTCAS RILENYESVF DATVVKKMKE
110 120 130 140 150
AGFVVVGKAN LDEFAMGSST ERSAFFPTRN PWDLERVPGG SSGGSAAAVS
160 170 180 190 200
AGMVVAALGS DTGGSVRQPA SLCGVVGYKP TYGLVSRYGL VAFASSLDQI
210 220 230 240 250
GPITKTVRDA AILMEIISGR DENDATTVNR KVDFLSEIEE GVSGMKFAVP
260 270 280 290 300
EEIYEHDIEE GVSERFEEAL KLLERLGAKV ERVKIPHIKY SVATYYVIAP
310 320 330 340 350
AEASSNLARF DGVKYGLRIK EKGLREMYMK TRNVGFGEEV RRRIMIGTFT
360 370 380 390 400
LSAAYYEAYF NKAMKVRRKI SDELNEVLSQ YDAILTPTSP VTAFKIGEIK
410 420 430 440 450
DPLTYYLMDI FTIPANLAGL PAISVPFGFS NNLPVGVQVI GRRFADGKVF
460 470
RIARAIEKNS PYNENGMFPL PEVKA
Length:475
Mass (Da):52,529
Last modified:November 1, 1999 - v1
Checksum:i4F01EA3A2DCBD9A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36347.1.
PIRiG72274.
RefSeqiNP_229077.1. NC_000853.1.
WP_010865293.1. NC_000853.1.

Genome annotation databases

EnsemblBacteriaiAAD36347; AAD36347; TM_1272.
GeneIDi898211.
KEGGitma:TM1272.
tmi:THEMA_07975.
PATRICi23937482. VBITheMar51294_1287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36347.1.
PIRiG72274.
RefSeqiNP_229077.1. NC_000853.1.
WP_010865293.1. NC_000853.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GI3X-ray1.80A1-475[»]
3AL0X-ray3.37A1-475[»]
ProteinModelPortaliQ9X0Z9.
SMRiQ9X0Z9. Positions 1-475.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59228N.
STRINGi243274.TM1272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36347; AAD36347; TM_1272.
GeneIDi898211.
KEGGitma:TM1272.
tmi:THEMA_07975.
PATRICi23937482. VBITheMar51294_1287.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
InParanoidiQ9X0Z9.
KOiK02433.
OMAiSKQYRER.
OrthoDBiEOG61P6R9.

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1303-MONOMER.
BRENDAi6.3.5.7. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X0Z9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiGATA_THEMA
AccessioniPrimary (citable) accession number: Q9X0Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 11, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.