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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Charge relay systemBy similarity1
Active sitei141Charge relay systemBy similarity1
Active sitei165Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.5.7. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A (EC:6.3.5.7)
Short name:
Glu-ADT subunit A
Gene namesi
Name:gatA
Ordered Locus Names:TM_1272
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001052211 – 475Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST475

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

DIPiDIP-59228N.
STRINGi243274.TM1272.

Structurei

Secondary structure

1475
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi11 – 14Combined sources4
Helixi19 – 22Combined sources4
Helixi25 – 40Combined sources16
Beta strandi43 – 46Combined sources4
Turni57 – 60Combined sources4
Beta strandi62 – 66Combined sources5
Beta strandi72 – 74Combined sources3
Helixi81 – 83Combined sources3
Helixi93 – 101Combined sources9
Beta strandi104 – 109Combined sources6
Helixi113 – 115Combined sources3
Beta strandi118 – 120Combined sources3
Beta strandi140 – 142Combined sources3
Helixi143 – 150Combined sources8
Beta strandi153 – 164Combined sources12
Helixi167 – 173Combined sources7
Beta strandi175 – 179Combined sources5
Turni195 – 197Combined sources3
Beta strandi199 – 206Combined sources8
Helixi207 – 217Combined sources11
Turni236 – 239Combined sources4
Beta strandi246 – 250Combined sources5
Helixi251 – 255Combined sources5
Helixi260 – 275Combined sources16
Beta strandi279 – 283Combined sources5
Helixi288 – 290Combined sources3
Helixi291 – 304Combined sources14
Beta strandi311 – 316Combined sources6
Helixi324 – 335Combined sources12
Helixi336 – 380Combined sources45
Beta strandi381 – 388Combined sources8
Beta strandi390 – 392Combined sources3
Turni396 – 398Combined sources3
Helixi402 – 406Combined sources5
Turni407 – 412Combined sources6
Helixi413 – 418Combined sources6
Beta strandi422 – 430Combined sources9
Beta strandi433 – 440Combined sources8
Helixi446 – 459Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GI3X-ray1.80A1-475[»]
3AL0X-ray3.37A1-475[»]
ProteinModelPortaliQ9X0Z9.
SMRiQ9X0Z9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X0Z9.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
InParanoidiQ9X0Z9.
KOiK02433.
OMAiSKQYRER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X0Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDLDFRKLT IEECLKLSEE EREKLPQLSL ETIKRLDPHV KAFISVRENV
60 70 80 90 100
SVEKKGKFWG IPVAIKDNIL TLGMRTTCAS RILENYESVF DATVVKKMKE
110 120 130 140 150
AGFVVVGKAN LDEFAMGSST ERSAFFPTRN PWDLERVPGG SSGGSAAAVS
160 170 180 190 200
AGMVVAALGS DTGGSVRQPA SLCGVVGYKP TYGLVSRYGL VAFASSLDQI
210 220 230 240 250
GPITKTVRDA AILMEIISGR DENDATTVNR KVDFLSEIEE GVSGMKFAVP
260 270 280 290 300
EEIYEHDIEE GVSERFEEAL KLLERLGAKV ERVKIPHIKY SVATYYVIAP
310 320 330 340 350
AEASSNLARF DGVKYGLRIK EKGLREMYMK TRNVGFGEEV RRRIMIGTFT
360 370 380 390 400
LSAAYYEAYF NKAMKVRRKI SDELNEVLSQ YDAILTPTSP VTAFKIGEIK
410 420 430 440 450
DPLTYYLMDI FTIPANLAGL PAISVPFGFS NNLPVGVQVI GRRFADGKVF
460 470
RIARAIEKNS PYNENGMFPL PEVKA
Length:475
Mass (Da):52,529
Last modified:November 1, 1999 - v1
Checksum:i4F01EA3A2DCBD9A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36347.1.
PIRiG72274.
RefSeqiNP_229077.1. NC_000853.1.
WP_010865293.1. NC_000853.1.

Genome annotation databases

EnsemblBacteriaiAAD36347; AAD36347; TM_1272.
GeneIDi898211.
KEGGitma:TM1272.
tmi:THEMA_07975.
PATRICi23937482. VBITheMar51294_1287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36347.1.
PIRiG72274.
RefSeqiNP_229077.1. NC_000853.1.
WP_010865293.1. NC_000853.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GI3X-ray1.80A1-475[»]
3AL0X-ray3.37A1-475[»]
ProteinModelPortaliQ9X0Z9.
SMRiQ9X0Z9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59228N.
STRINGi243274.TM1272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36347; AAD36347; TM_1272.
GeneIDi898211.
KEGGitma:TM1272.
tmi:THEMA_07975.
PATRICi23937482. VBITheMar51294_1287.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
InParanoidiQ9X0Z9.
KOiK02433.
OMAiSKQYRER.

Enzyme and pathway databases

BRENDAi6.3.5.7. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X0Z9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_THEMA
AccessioniPrimary (citable) accession number: Q9X0Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.