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Protein

[FeFe] hydrogenase maturase subunit HydE

Gene

TM_1269

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the maturation of the [FeFe]-hydrogenase HydA (By similarity). Catalyzes the reductive cleavage of S-adenosyl-L-methionine (in vitro), suggesting it may contribute to the biosynthesis of an essential sulfur-containing ligand that binds to the hydrogenase active site [2Fe-2S] cluster (PubMed:16137685).By similarityCurated1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotationCombined sources3 Publications1
Metal bindingi67Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotationCombined sources1 Publication1
Metal bindingi70Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotationCombined sources1 Publication1
Metal bindingi311Iron-sulfur (2Fe-2S)Combined sources1
Metal bindingi319Iron-sulfur (2Fe-2S)Combined sources1
Metal bindingi322Iron-sulfur (2Fe-2S)Combined sources1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
[FeFe] hydrogenase maturase subunit HydECurated (EC:1.8.-.-Curated)
Gene namesi
Ordered Locus Names:TM_1269Imported
ORF Names:THEMA_07990Imported, Tmari_1274Imported
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)Imported
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000013901 Componenti: Chromosome
  • UP000008183 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63C → A: Eliminates binding of one iron-sulfur cluster; when associated with A-67 and A-70. 1 Publication1
Mutagenesisi67C → A: Eliminates binding of one iron-sulfur cluster; when associated with A-63 and A-70. 1 Publication1
Mutagenesisi70C → A: Eliminates binding of one iron-sulfur cluster; when associated with A-63 and A-67. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004332301 – 348[FeFe] hydrogenase maturase subunit HydEAdd BLAST348

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi243274.TM1269.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi17 – 25Combined sources9
Helixi29 – 47Combined sources19
Beta strandi49 – 60Combined sources12
Helixi86 – 98Combined sources13
Beta strandi102 – 109Combined sources8
Helixi112 – 114Combined sources3
Helixi117 – 127Combined sources11
Turni128 – 130Combined sources3
Beta strandi132 – 136Combined sources5
Helixi142 – 151Combined sources10
Beta strandi155 – 157Combined sources3
Helixi165 – 171Combined sources7
Helixi177 – 189Combined sources13
Beta strandi193 – 196Combined sources4
Beta strandi198 – 201Combined sources4
Helixi207 – 220Combined sources14
Beta strandi223 – 225Combined sources3
Turni237 – 240Combined sources4
Helixi246 – 259Combined sources14
Helixi269 – 274Combined sources6
Helixi278 – 283Combined sources6
Turni284 – 286Combined sources3
Turni297 – 299Combined sources3
Helixi300 – 302Combined sources3
Beta strandi305 – 308Combined sources4
Beta strandi312 – 314Combined sources3
Helixi319 – 329Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CIWX-ray1.35A1-348[»]
3CIXX-ray1.70A1-348[»]
3IIXX-ray1.25A1-348[»]
3IIZX-ray1.62A1-348[»]
4JXCX-ray1.50A1-348[»]
4JY8X-ray2.90A2-348[»]
4JY9X-ray1.60A1-348[»]
4JYDX-ray1.71A1-348[»]
4JYEX-ray1.65A1-348[»]
4JYFX-ray1.45A1-348[»]
5FEPX-ray1.45A2-348[»]
5FESX-ray1.27A2-348[»]
5FEWX-ray1.17A2-348[»]
5FEXX-ray1.32A2-348[»]
5FEZX-ray1.35A2-348[»]
5FF0X-ray1.49A2-348[»]
5FF2X-ray1.47A1-348[»]
5FF3X-ray1.18A1-348[»]
5FF4X-ray1.35A2-348[»]
ProteinModelPortaliQ9X0Z6.
SMRiQ9X0Z6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. HydE family.Curated

Phylogenomic databases

eggNOGiENOG4107QW8. Bacteria.
COG0502. LUCA.
KOiK01012.
OMAiIDMIGMG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR006638. Elp3/MiaB/NifB.
IPR024021. FeFe-hyd_HydE_rSAM.
IPR005244. FO_synthase_su2-like.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF004762. CHP00423. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03956. rSAM_HydE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X0Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGREILEKL ERREFTREVL KEALSINDRG FNEALFKLAD EIRRKYVGDE
60 70 80 90 100
VHIRAIIEFS NVCRKNCLYC GLRRDNKNLK RYRMTPEEIV ERARLAVQFG
110 120 130 140 150
AKTIVLQSGE DPYYMPDVIS DIVKEIKKMG VAVTLSLGEW PREYYEKWKE
160 170 180 190 200
AGADRYLLRH ETANPVLHRK LRPDTSFENR LNCLLTLKEL GYETGAGSMV
210 220 230 240 250
GLPGQTIDDL VDDLLFLKEH DFDMVGIGPF IPHPDTPLAN EKKGDFTLTL
260 270 280 290 300
KMVALTRILL PDSNIPATTA MGTIVPGGRE ITLRCGANVI MPNWTPSPYR
310 320 330 340
QLYQLYPGKI CVFEKDTACI PCVMKMIELL GRKPGRDWGG RKRVFETV
Length:348
Mass (Da):39,799
Last modified:November 1, 1999 - v1
Checksum:i472505213FF63799
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36344.1.
CP004077 Genomic DNA. Translation: AGL50198.1.
CP007013 Genomic DNA. Translation: AHD18826.1.
PIRiD72274.
RefSeqiNP_229074.1. NC_000853.1.
WP_004079975.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36344; AAD36344; TM_1269.
AGL50198; AGL50198; Tmari_1274.
AHD18826; AHD18826; THEMA_07990.
GeneIDi898214.
KEGGitma:TM1269.
tmi:THEMA_07990.
tmm:Tmari_1274.
tmw:THMA_1294.
PATRICi23937478. VBITheMar51294_1285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36344.1.
CP004077 Genomic DNA. Translation: AGL50198.1.
CP007013 Genomic DNA. Translation: AHD18826.1.
PIRiD72274.
RefSeqiNP_229074.1. NC_000853.1.
WP_004079975.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CIWX-ray1.35A1-348[»]
3CIXX-ray1.70A1-348[»]
3IIXX-ray1.25A1-348[»]
3IIZX-ray1.62A1-348[»]
4JXCX-ray1.50A1-348[»]
4JY8X-ray2.90A2-348[»]
4JY9X-ray1.60A1-348[»]
4JYDX-ray1.71A1-348[»]
4JYEX-ray1.65A1-348[»]
4JYFX-ray1.45A1-348[»]
5FEPX-ray1.45A2-348[»]
5FESX-ray1.27A2-348[»]
5FEWX-ray1.17A2-348[»]
5FEXX-ray1.32A2-348[»]
5FEZX-ray1.35A2-348[»]
5FF0X-ray1.49A2-348[»]
5FF2X-ray1.47A1-348[»]
5FF3X-ray1.18A1-348[»]
5FF4X-ray1.35A2-348[»]
ProteinModelPortaliQ9X0Z6.
SMRiQ9X0Z6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36344; AAD36344; TM_1269.
AGL50198; AGL50198; Tmari_1274.
AHD18826; AHD18826; THEMA_07990.
GeneIDi898214.
KEGGitma:TM1269.
tmi:THEMA_07990.
tmm:Tmari_1274.
tmw:THMA_1294.
PATRICi23937478. VBITheMar51294_1285.

Phylogenomic databases

eggNOGiENOG4107QW8. Bacteria.
COG0502. LUCA.
KOiK01012.
OMAiIDMIGMG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR006638. Elp3/MiaB/NifB.
IPR024021. FeFe-hyd_HydE_rSAM.
IPR005244. FO_synthase_su2-like.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF004762. CHP00423. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03956. rSAM_HydE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHYDE_THEMA
AccessioniPrimary (citable) accession number: Q9X0Z6
Secondary accession number(s): G4FE79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.