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Protein

Glutamine-dependent NAD(+) synthetase

Gene

nadE2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (nadE2), Glutamine-dependent NAD(+) synthetase (Tmari_1258)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Proton acceptor; for glutaminase activityUniRule annotation1
Active sitei112For glutaminase activityUniRule annotation1
Binding sitei118L-glutamineUniRule annotation1
Active sitei148Nucleophile; for glutaminase activityUniRule annotation1
Binding sitei176L-glutamineUniRule annotation1
Binding sitei182L-glutamineUniRule annotation1
Binding sitei404Deamido-NADUniRule annotation1
Binding sitei428ATPUniRule annotation1
Binding sitei433Deamido-NADUniRule annotation1
Binding sitei545Deamido-NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi321 – 328ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.5.1UniRule annotation)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]UniRule annotation
Gene namesi
Name:nadE2
Ordered Locus Names:TM_1253
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001522451 – 576Glutamine-dependent NAD(+) synthetaseAdd BLAST576

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1253.

Structurei

3D structure databases

ProteinModelPortaliQ9X0Y0.
SMRiQ9X0Y0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 246CN hydrolasePROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni292 – 576LigaseAdd BLAST285

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
COG0388. LUCA.
InParanoidiQ9X0Y0.
KOiK01950.
OMAiRLAQDCY.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
HAMAPiMF_02090. NadE_glutamine_dep. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR000132. Nitrilase/CN_hydratase_CS.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR23090. PTHR23090. 1 hit.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X0Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLRVTLAQ LNPTLGDFEG NLKKAIEALR VAEDRGSDLL VFPELFLPGY
60 70 80 90 100
PPEDLMLRLS FLRENRKYLQ KFAQHTRNLG VTVLMGFIDS DEDAYNAAAV
110 120 130 140 150
VKDGEILGVY RKISLPNYGV FDERRYFKPG EELLVVKIGN IKVGVTICED
160 170 180 190 200
IWNPVEPSAS LSLGEGVHLI ANLSASPYHV GKPVLRKDYL SMKAYDYHVA
210 220 230 240 250
MAYCNMVGGQ DELVFDGGSM VVDASGEVIN YGKLFEEEII TVDLDLDENL
260 270 280 290 300
RVSLVDPRRR YMKTQNYPVK TVEAGNLREK SGHFEPVVNP LPVREEEMFR
310 320 330 340 350
ALITGLRDYV RKNGFEKVVI GLSGGMDSSL VAVIATEALG KENVKGVLMP
360 370 380 390 400
SMYTSKESIE DAQTLAKNLG IETFIIPITD VFHSYLETLK GVFAGREPDI
410 420 430 440 450
TEENLQARIR GNYLMALSNK FGWLVLTTGN KSEMATGYAT LYGDMAGGFA
460 470 480 490 500
VIKDVYKTDV YRIGRWYNSW RGKEIIPENI FVKPPTAELR PGQTDQEKLP
510 520 530 540 550
PYEVLDEILR LYIEEGLDPE EIASKGFDRK TVLDVTEMIR KNEYKRKQAA
560 570
IGVKISTRAF GKDWRMPITN RFKEPL
Length:576
Mass (Da):64,920
Last modified:November 1, 1999 - v1
Checksum:i208FDC77964C957F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36328.1.
PIRiG72277.
RefSeqiNP_229058.1. NC_000853.1.
WP_004080010.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36328; AAD36328; TM_1253.
GeneIDi898230.
KEGGitma:TM1253.

Similar proteinsi

Entry informationi

Entry nameiNADE2_THEMA
AccessioniPrimary (citable) accession number: Q9X0Y0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: October 25, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families