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Q9X0X7 (PUR2_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:TM_1250
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151495

Regions

Domain99 – 303205ATP-grasp
Nucleotide binding125 – 18662ATP By similarity

Sites

Metal binding2731Magnesium or manganese By similarity
Metal binding2751Magnesium or manganese By similarity

Secondary structure

................................................................ 400
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9X0X7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 411CE014DD7DE90F

FASTA40044,326
        10         20         30         40         50         60 
MKAVRVHILG SGGREHAIGW AFAKQGYEVH FYPGNAGTKR DGTNHPYEGE KTLKAIPEED 

        70         80         90        100        110        120 
IVIPGSEEFL VEGVSNWRSN VFGPVKEVAR LEGSKVYAKR FMKKYGIRTA RFEVAETPEE 

       130        140        150        160        170        180 
LREKIKKFSP PYVIKADGLA RGKGVLILDS KEETIEKGSK LIIGELIKGV KGPVVIDEFL 

       190        200        210        220        230        240 
AGNELSAMAV VNGRNFVILP FVRDYKRLMD GDRGPNTGGM GSWGPVEIPS DTIKKIEELF 

       250        260        270        280        290        300 
DKTLWGVEKE GYAYRGFLYL GLMLHDGDPY ILEYNVRLGD PETEVIVTLN PEGFVNAVLE 

       310        320        330        340        350        360 
GYRGGKMEPV EPRGFAVDVV LAARGYPDAP EKGKEITLPE EGLIFFAGVA EKDGKLVTNG 

       370        380        390        400 
GRVLHCMGTG ETKEEARRKA YELAEKVHFE GKTYRRDIAL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD36325.1.
PIRD72277.
RefSeqNP_229055.1. NC_000853.1.
YP_008991653.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VKZX-ray2.30A/B1-400[»]
ProteinModelPortalQ9X0X7.
SMRQ9X0X7. Positions 4-399.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM1250.

Protocols and materials databases

DNASU898233.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36325; AAD36325; TM_1250.
GeneID18093463.
898233.
KEGGtma:TM1250.
PATRIC23937440. VBITheMar51294_1266.

Phylogenomic databases

eggNOGCOG0151.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBCLSK875656.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-1281-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9X0X7.

Entry information

Entry namePUR2_THEMA
AccessionPrimary (citable) accession number: Q9X0X7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: March 19, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways