Reviewed, UniProtKB/Swiss-Prot Q9X0X0 (PUR7_THEMA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	TM_1243

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 4/5. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 230

230

Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137

PRO_0000100891

Secondary structure

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230

Helix Strand Turn

Details...

Beta strand

8 – 13

6

Beta strand

16 – 21

6

Helix

39 – 56

18

Beta strand

61 – 67

7

Turn

68 – 70

3

Beta strand

71 – 74

4

Beta strand

81 – 89

9

Helix

92 – 98

7

Beta strand

105 – 115

11

Helix

118 – 120

3

Helix

127 – 132

6

Helix

138 – 160

23

Turn

161 – 163

3

Beta strand

164 – 171

8

Beta strand

173 – 175

3

Beta strand

181 – 183

3

Turn

189 – 191

3

Beta strand

192 – 196

5

Helix

203 – 208

6

Helix

209 – 211

3

Helix

215 – 227

13

Sequences

	Sequence		Length	Mass (Da)
	Q9X0X0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 242B18AD026DB4A9	FASTA	230	26,231
	10 20 30 40 50 60 MNYEGKTKIV KVTGDYALLE FKDDITAGDG LKHDVLTGKG SICAETTAIL MKYLSEKGIK 70 80 90 100 110 120 THLVEYIPPR TLKVIPLKMF PLEVVVRLKK AGSFVRRYGG AEGEDLPVPL VEFFIKDDER 130 140 150 160 170 180 HDPMVCVDHL EILGIATKKQ AEKMKEAAVK ITLALKEFFE RANFELWDIK YEFGLDKDGN 190 200 210 220 230 VVLGDEISPD TFRLRKKGEI FDKDVYRRDL GDPLKKYREV LELCRSLNSQ

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000512 Genomic DNA. Translation: AAD36318.1.

PIR

E72276.

RefSeq

NP_229048.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KUT	X-ray	2.20	A/B	1-230	[»]

ModBase

Search...

Genome annotation databases

GeneID

898240.

GenomeReviews

Gene locus TM_1243 in contig AE000512_GR.

KEGG

tma:TM1243.

NMPDR

fig|243274.1.peg.1232.

TIGR

TM_1243.

Phylogenomic databases

HOGENOM

Q9X0X0.

OMA

Q9X0X0. TAFNAQK.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_1243-MON.

BRENDA

6.3.2.6. 16699.

Family and domain databases

HAMAP

MF_00137.
[Tree]

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synt.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

PANTHER

PTHR11609. SAICAR_synt. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

ProDom

PD003043. SAICAR_synt. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PUR7_THEMA

Accession

Primary (citable) accession number: Q9X0X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)