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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene

TM_1201

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi469 – 4691Calcium; via carbonyl oxygenCombined sources
Metal bindingi471 – 4711CalciumCombined sources
Binding sitei497 – 4971GalactoseCombined sources
Metal bindingi500 – 5001CalciumCombined sources
Metal bindingi503 – 5031Calcium; via carbonyl oxygenCombined sources
Binding sitei563 – 5631GalactoseCombined sources
Binding sitei594 – 5941GalactoseCombined sources
Metal bindingi598 – 5981CalciumCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1231-MONOMER.
BRENDAi3.2.1.89. 6331.

Protein family/group databases

CAZyiCBM61. Carbohydrate-Binding Module Family 61.
GH53. Glycoside Hydrolase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanaseUniRule annotation (EC:3.2.1.89UniRule annotation)
Gene namesi
Ordered Locus Names:TM_1201Imported
ORF Names:Tmari_1208Imported
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)Imported
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000013901 Componenti: Chromosome
  • UP000008183 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1201.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XOMX-ray0.95A461-606[»]
2XONX-ray1.40A/L461-606[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 375346Glyco_hydro_53InterPro annotationAdd
BLAST
Domaini393 – 44654Big_4InterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni506 – 5083Galactose bindingCombined sources

Sequence similaritiesi

Belongs to the glycosyl hydrolase 53 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106FKC. Bacteria.
COG3867. LUCA.
KOiK01224.
OMAiTGWLEWK.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011081. Big_4.
IPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07532. Big_4. 1 hit.
PF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X0S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGVLFVLMI SSMAFGLIVN PVENLREDFI FGMDVSMLYE IEQLGGKYFE
60 70 80 90 100
NGVEKDCLEI LKNHGINWIR LRVWNDPRDE NGNPLGGGNC DYLKMTEIAK
110 120 130 140 150
RAKNLGMKVL LDFHYSDWWA DPGKQNKPKE WEYLHGELLE RAVYSYTKLV
160 170 180 190 200
LNHMRRNGAL PDMVQVGNEV NNGFLWPDGK ISGEGAGGFD GFTRLLKAAI
210 220 230 240 250
KAVREVDPDI KIVIHLAEGG NNSLFRWFFD EITRRNVDFD VIGVSYYPYW
260 270 280 290 300
HGTLEDLKNN LYDIATRYNK DVLVVETAYA WTLEDGDGYP NIFNGEEMEL
310 320 330 340 350
TGGYKATVQG QATFLRDLIE VVNSVPNGHG LGIFYWEGDW IPVRGAGWKT
360 370 380 390 400
GEGNPWDNQA MFDFSGNALP SLNVFKLVKT SSPVEIAIKE ILPVEVTTNL
410 420 430 440 450
GEVPKFPDAV KVLFSDDSIR SLPVEWNFDS ALVEESGVYK VEGYIKDIDR
460 470 480 490 500
KIFATLTVKG SRNYLKNPGF ETGEFSPWRV SGDKKAVKVV KANPSSNAHQ
510 520 530 540 550
GEYAVNFWLD ESFSFELSQE VELPAGVYRV GFWTHGEKGV KIALKVSDYG
560 570 580 590 600
GNERSVEVET TGWLEWKNPE IRNIKVETGR IKITVSVEGR AGDWGFIDDF

YLFREE
Length:606
Mass (Da):68,620
Last modified:November 1, 1999 - v1
Checksum:i0A4C121D93ABCB00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36276.1.
CP004077 Genomic DNA. Translation: AGL50132.1.
PIRiG72282.
RefSeqiNP_229006.1. NC_000853.1.
WP_004080113.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36276; AAD36276; TM_1201.
AGL50132; AGL50132; Tmari_1208.
GeneIDi898283.
KEGGitma:TM1201.
tmi:THEMA_08325.
tmm:Tmari_1208.
tmw:THMA_1227.
PATRICi23937344. VBITheMar51294_1219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36276.1.
CP004077 Genomic DNA. Translation: AGL50132.1.
PIRiG72282.
RefSeqiNP_229006.1. NC_000853.1.
WP_004080113.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XOMX-ray0.95A461-606[»]
2XONX-ray1.40A/L461-606[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1201.

Protein family/group databases

CAZyiCBM61. Carbohydrate-Binding Module Family 61.
GH53. Glycoside Hydrolase Family 53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36276; AAD36276; TM_1201.
AGL50132; AGL50132; Tmari_1208.
GeneIDi898283.
KEGGitma:TM1201.
tmi:THEMA_08325.
tmm:Tmari_1208.
tmw:THMA_1227.
PATRICi23937344. VBITheMar51294_1219.

Phylogenomic databases

eggNOGiENOG4106FKC. Bacteria.
COG3867. LUCA.
KOiK01224.
OMAiTGWLEWK.

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1231-MONOMER.
BRENDAi3.2.1.89. 6331.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011081. Big_4.
IPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07532. Big_4. 1 hit.
PF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ9X0S8_THEMA
AccessioniPrimary (citable) accession number: Q9X0S8
Secondary accession number(s): G4FEE5
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1999
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.