Q9X0L4 (PTAS_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphate acetyltransferase
EC=2.3.1.8

Alternative name(s):
Phosphotransacetylase

Gene names

Name:	pta
Ordered Locus Names:	TM_1130

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In addition to acetyl-CoA (100%), the enzyme accepts propionyl-CoA (60%) and butyryl-CoA (30%).
Catalytic activity	Acetyl-CoA + phosphate = CoA + acetyl phosphate.
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the phosphate acetyltransferase and butyryltransferase family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 90 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphate acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Phosphate acetyltransferase		PRO_0000179149
Experimental info
	Sequence conflict	11	1	R → Y AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9X0L4 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F4B98B3CAE120AFB	FASTA	294	32,093
	10 20 30 40 50 60 MFLEKLVEMA RGKGKKLAVA AANDDHVIEA VYRAWRERVC EPVLFGPEEE ITRIIEELVP 70 80 90 100 110 120 EWKNPQIIDC PPEEAGRLAV EAVSKGECDF LMKGKIKTGD LMKIYLDERY GLRTGKTMAM 130 140 150 160 170 180 VSVMEIPDFP RPLIISDPGM LISPTLEQKV DMIEHCVRVA NVMGLETPKV AVVGAIEVVN 190 200 210 220 230 240 PKMPITMEAA ILSKMNQRGQ IKGCIVDGPF ALDNVVSEEA AKKKGIQSPV AGKADILILP 250 260 270 280 290 DIEAANILYK ALVFLAKAKS ASTILGGKVP VVLTSRADSE ETKFYSIALS AVFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima." Bock A.-K., Glasemacher J., Schmidt R., Schoenheit P. J. Bacteriol. 181:1861-1867(1999) [PubMed: 10074080] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-39, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD36206.1.
PIR	G72293.
RefSeq	NP_228936.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9X0L4.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2900N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	898634.
GenomeReviews	Gene locus TM_1130 in contig AE000512_GR.
KEGG	tma:TM1130.
NMPDR	fig|243274.1.peg.1120.
PATRIC	23937195. VBITheMar51294_1146.
TIGR	TM_1130.
Phylogenomic databases
HOGENOM	HBG294533.
OMA	RGQIKDC.
PhylomeDB	Q9X0L4.
ProtClustDB	CLSK795703.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-427. TMAR243274:TM_1130-MONOMER.
Family and domain databases
InterPro	IPR012147. P_Ac_Bu_trans. IPR002505. PTA_PTB. [Graphical view]
KO	K00625.
Pfam	PF01515. PTA_PTB. 2 hits. [Graphical view]
PIRSF	PIRSF000428. P_Ac_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTAS_THEMA

Accession

Primary (citable) accession number: Q9X0L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program