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Protein

Ribonuclease 3

Gene

rnc

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 40-70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54MagnesiumBy similarity1
Active sitei58Sequence analysis1
Metal bindingi127MagnesiumBy similarity1
Active sitei130By similarity1
Metal bindingi130MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.26.3. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:TM_1102
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001804481 – 240Ribonuclease 3Add BLAST240

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1102.

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Helixi23 – 30Combined sources8
Helixi33 – 41Combined sources9
Helixi51 – 72Combined sources22
Helixi78 – 88Combined sources11
Helixi91 – 100Combined sources10
Helixi103 – 106Combined sources4
Helixi111 – 115Combined sources5
Helixi118 – 120Combined sources3
Helixi122 – 140Combined sources19
Helixi142 – 161Combined sources20
Helixi169 – 181Combined sources13
Beta strandi186 – 193Combined sources8
Beta strandi200 – 207Combined sources8
Beta strandi210 – 219Combined sources10
Helixi220 – 235Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O0WX-ray2.00A/B1-240[»]
ProteinModelPortaliQ9X0I6.
SMRiQ9X0I6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X0I6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 141RNase IIIAdd BLAST133
Domaini168 – 237DRBMAdd BLAST70

Sequence similaritiesi

Belongs to the ribonuclease III family.Curated

Phylogenomic databases

eggNOGiENOG4108ZBM. Bacteria.
COG0571. LUCA.
InParanoidiQ9X0I6.
KOiK03685.
OMAiLTHKSCK.

Family and domain databases

CDDicd00593. RIBOc. 1 hit.
Gene3Di1.10.1520.10. 1 hit.
HAMAPiMF_00104. RNase_III. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
PfamiView protein in Pfam
PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X0I6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNESERKIVE EFQKETGINF KNEELLFRAL CHSSYANEQN QAGRKDVESN
60 70 80 90 100
EKLEFLGDAV LELFVCEILY KKYPEAEVGD LARVKSAAAS EEVLAMVSRK
110 120 130 140 150
MNLGKFLFLG KGEEKTGGRD RDSILADAFE ALLAAIYLDQ GYEKIKELFE
160 170 180 190 200
QEFEFYIEKI MKGEMLFDYK TALQEIVQSE HKVPPEYILV RTEKNDGDRI
210 220 230 240
FVVEVRVNGK TIATGKGRTK KEAEKEAARI AYEKLLKERS
Length:240
Mass (Da):27,530
Last modified:November 1, 1999 - v1
Checksum:i94330E8898D48A0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36178.1.
PIRiH72294.
RefSeqiNP_228908.1. NC_000853.1.
WP_004080335.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36178; AAD36178; TM_1102.
GeneIDi898663.
KEGGitma:TM1102.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRNC_THEMA
AccessioniPrimary (citable) accession number: Q9X0I6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: July 5, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families