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Q9X0I6 (RNC_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:TM_1102
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Ref.2

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Magnesium. Ref.2

Subunit structure

Homodimer Probable. Ref.3

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.0. Ref.2

Temperature dependence:

Optimum temperature is 40-70 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000180448

Regions

Domain9 – 141133RNase III
Domain168 – 23770DRBM

Sites

Active site581 Potential
Active site1301 By similarity
Metal binding541Magnesium By similarity
Metal binding1271Magnesium By similarity
Metal binding1301Magnesium By similarity

Secondary structure

................................ 240
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9X0I6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 94330E8898D48A0D

FASTA24027,530
        10         20         30         40         50         60 
MNESERKIVE EFQKETGINF KNEELLFRAL CHSSYANEQN QAGRKDVESN EKLEFLGDAV 

        70         80         90        100        110        120 
LELFVCEILY KKYPEAEVGD LARVKSAAAS EEVLAMVSRK MNLGKFLFLG KGEEKTGGRD 

       130        140        150        160        170        180 
RDSILADAFE ALLAAIYLDQ GYEKIKELFE QEFEFYIEKI MKGEMLFDYK TALQEIVQSE 

       190        200        210        220        230        240 
HKVPPEYILV RTEKNDGDRI FVVEVRVNGK TIATGKGRTK KEAEKEAARI AYEKLLKERS 

« Hide

References

« Hide 'large scale' references
[1]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Thermotoga maritima ribonuclease III. Characterization of thermostable biochemical behavior and analysis of conserved base pairs that function as reactivity epitopes for the Thermotoga 23S rRNA precursor."
Nathania L., Nicholson A.W.
Biochemistry 49:7164-7178(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN RNASE, FUNCTION AS AN ENDONUCLEASE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, RRNA-BINDING.
[3]"Crystal structure of ribonuclease III (TM1102) from Thermotoga maritima at 2.0 A resolution."
Joint center for structural genomics (JCSG)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD36178.1.
PIRH72294.
RefSeqNP_228908.1. NC_000853.1.
YP_007977457.1. NC_021214.1.
YP_008991800.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1O0WX-ray2.00A/B1-240[»]
ProteinModelPortalQ9X0I6.
SMRQ9X0I6. Positions 1-237.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM1102.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36178; AAD36178; TM_1102.
GeneID15494710.
18093614.
898663.
KEGGtma:TM1102.
tmm:Tmari_1108.
PATRIC23937137. VBITheMar51294_1117.

Phylogenomic databases

eggNOGCOG0571.
KOK03685.
OMATETNFKS.
OrthoDBEOG6T1WVS.
ProtClustDBPRK00102.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9X0I6.

Entry information

Entry nameRNC_THEMA
AccessionPrimary (citable) accession number: Q9X0I6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references