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Q9X0I6

- RNC_THEMA

UniProt

Q9X0I6 - RNC_THEMA

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Protein

Ribonuclease 3

Gene

rnc

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Magnesium.1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 40-70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541MagnesiumBy similarity
Active sitei58 – 581Sequence Analysis
Metal bindingi127 – 1271MagnesiumBy similarity
Active sitei130 – 1301By similarity
Metal bindingi130 – 1301MagnesiumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:TM_1102
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Ribonuclease 3PRO_0000180448Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1102.

Structurei

Secondary structure

1
240
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1614
Helixi23 – 308
Helixi33 – 419
Helixi51 – 7222
Helixi78 – 8811
Helixi91 – 10010
Helixi103 – 1064
Helixi111 – 1155
Helixi118 – 1203
Helixi122 – 14019
Helixi142 – 16120
Helixi169 – 18113
Beta strandi186 – 1938
Beta strandi200 – 2078
Beta strandi210 – 21910
Helixi220 – 23516

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O0WX-ray2.00A/B1-240[»]
ProteinModelPortaliQ9X0I6.
SMRiQ9X0I6. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X0I6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 141133RNase IIIAdd
BLAST
Domaini168 – 23770DRBMAdd
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.Curated

Phylogenomic databases

eggNOGiCOG0571.
InParanoidiQ9X0I6.
KOiK03685.
OMAiYGFQEAR.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X0I6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNESERKIVE EFQKETGINF KNEELLFRAL CHSSYANEQN QAGRKDVESN
60 70 80 90 100
EKLEFLGDAV LELFVCEILY KKYPEAEVGD LARVKSAAAS EEVLAMVSRK
110 120 130 140 150
MNLGKFLFLG KGEEKTGGRD RDSILADAFE ALLAAIYLDQ GYEKIKELFE
160 170 180 190 200
QEFEFYIEKI MKGEMLFDYK TALQEIVQSE HKVPPEYILV RTEKNDGDRI
210 220 230 240
FVVEVRVNGK TIATGKGRTK KEAEKEAARI AYEKLLKERS
Length:240
Mass (Da):27,530
Last modified:November 1, 1999 - v1
Checksum:i94330E8898D48A0D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36178.1.
PIRiH72294.
RefSeqiNP_228908.1. NC_000853.1.
WP_004080335.1. NC_023151.1.
YP_007977457.1. NC_021214.1.
YP_008991800.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD36178; AAD36178; TM_1102.
GeneIDi18093614.
898663.
KEGGitma:TM1102.
tmi:THEMA_08835.
tmm:Tmari_1108.
PATRICi23937137. VBITheMar51294_1117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD36178.1 .
PIRi H72294.
RefSeqi NP_228908.1. NC_000853.1.
WP_004080335.1. NC_023151.1.
YP_007977457.1. NC_021214.1.
YP_008991800.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1O0W X-ray 2.00 A/B 1-240 [» ]
ProteinModelPortali Q9X0I6.
SMRi Q9X0I6. Positions 1-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM1102.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36178 ; AAD36178 ; TM_1102 .
GeneIDi 18093614.
898663.
KEGGi tma:TM1102.
tmi:THEMA_08835.
tmm:Tmari_1108.
PATRICi 23937137. VBITheMar51294_1117.

Phylogenomic databases

eggNOGi COG0571.
InParanoidi Q9X0I6.
KOi K03685.
OMAi YGFQEAR.
OrthoDBi EOG6T1WVS.

Miscellaneous databases

EvolutionaryTracei Q9X0I6.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Thermotoga maritima ribonuclease III. Characterization of thermostable biochemical behavior and analysis of conserved base pairs that function as reactivity epitopes for the Thermotoga 23S rRNA precursor."
    Nathania L., Nicholson A.W.
    Biochemistry 49:7164-7178(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN RNASE, FUNCTION AS AN ENDONUCLEASE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, RRNA-BINDING.
  3. "Crystal structure of ribonuclease III (TM1102) from Thermotoga maritima at 2.0 A resolution."
    Joint center for structural genomics (JCSG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiRNC_THEMA
AccessioniPrimary (citable) accession number: Q9X0I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3