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Q9X0I6

- RNC_THEMA

UniProt

Q9X0I6 - RNC_THEMA

Protein

Ribonuclease 3

Gene

rnc

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.1 Publication

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Magnesium.1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40-70 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541MagnesiumBy similarity
    Active sitei58 – 581Sequence Analysis
    Metal bindingi127 – 1271MagnesiumBy similarity
    Active sitei130 – 1301By similarity
    Metal bindingi130 – 1301MagnesiumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-HAMAP
    2. rRNA catabolic process Source: InterPro
    3. rRNA processing Source: UniProtKB-HAMAP
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3 (EC:3.1.26.3)
    Alternative name(s):
    Ribonuclease III
    Short name:
    RNase III
    Gene namesi
    Name:rnc
    Ordered Locus Names:TM_1102
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240Ribonuclease 3PRO_0000180448Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi243274.TM1102.

    Structurei

    Secondary structure

    1
    240
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1614
    Helixi23 – 308
    Helixi33 – 419
    Helixi51 – 7222
    Helixi78 – 8811
    Helixi91 – 10010
    Helixi103 – 1064
    Helixi111 – 1155
    Helixi118 – 1203
    Helixi122 – 14019
    Helixi142 – 16120
    Helixi169 – 18113
    Beta strandi186 – 1938
    Beta strandi200 – 2078
    Beta strandi210 – 21910
    Helixi220 – 23516

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1O0WX-ray2.00A/B1-240[»]
    ProteinModelPortaliQ9X0I6.
    SMRiQ9X0I6. Positions 1-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X0I6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 141133RNase IIIAdd
    BLAST
    Domaini168 – 23770DRBMAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RNase III domain.Curated

    Phylogenomic databases

    eggNOGiCOG0571.
    KOiK03685.
    OMAiYGFQEAR.
    OrthoDBiEOG6T1WVS.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X0I6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNESERKIVE EFQKETGINF KNEELLFRAL CHSSYANEQN QAGRKDVESN    50
    EKLEFLGDAV LELFVCEILY KKYPEAEVGD LARVKSAAAS EEVLAMVSRK 100
    MNLGKFLFLG KGEEKTGGRD RDSILADAFE ALLAAIYLDQ GYEKIKELFE 150
    QEFEFYIEKI MKGEMLFDYK TALQEIVQSE HKVPPEYILV RTEKNDGDRI 200
    FVVEVRVNGK TIATGKGRTK KEAEKEAARI AYEKLLKERS 240
    Length:240
    Mass (Da):27,530
    Last modified:November 1, 1999 - v1
    Checksum:i94330E8898D48A0D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36178.1.
    PIRiH72294.
    RefSeqiNP_228908.1. NC_000853.1.
    YP_007977457.1. NC_021214.1.
    YP_008991800.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36178; AAD36178; TM_1102.
    GeneIDi898663.
    KEGGitma:TM1102.
    tmi:THEMA_08835.
    tmm:Tmari_1108.
    PATRICi23937137. VBITheMar51294_1117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD36178.1 .
    PIRi H72294.
    RefSeqi NP_228908.1. NC_000853.1.
    YP_007977457.1. NC_021214.1.
    YP_008991800.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1O0W X-ray 2.00 A/B 1-240 [» ]
    ProteinModelPortali Q9X0I6.
    SMRi Q9X0I6. Positions 1-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1102.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36178 ; AAD36178 ; TM_1102 .
    GeneIDi 898663.
    KEGGi tma:TM1102.
    tmi:THEMA_08835.
    tmm:Tmari_1108.
    PATRICi 23937137. VBITheMar51294_1117.

    Phylogenomic databases

    eggNOGi COG0571.
    KOi K03685.
    OMAi YGFQEAR.
    OrthoDBi EOG6T1WVS.

    Miscellaneous databases

    EvolutionaryTracei Q9X0I6.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Thermotoga maritima ribonuclease III. Characterization of thermostable biochemical behavior and analysis of conserved base pairs that function as reactivity epitopes for the Thermotoga 23S rRNA precursor."
      Nathania L., Nicholson A.W.
      Biochemistry 49:7164-7178(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN RNASE, FUNCTION AS AN ENDONUCLEASE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, RRNA-BINDING.
    3. "Crystal structure of ribonuclease III (TM1102) from Thermotoga maritima at 2.0 A resolution."
      Joint center for structural genomics (JCSG)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiRNC_THEMA
    AccessioniPrimary (citable) accession number: Q9X0I6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3