Reviewed, UniProtKB/Swiss-Prot Q9X0I6 (RNC_THEMA)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	TM_1102

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: HAMAP rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

Ribonuclease 3 HAMAP MF_00104

PRO_0000180448

Regions

Domain

9 – 141

133

RNase III

Domain

168 – 237

70

DRBM

Secondary structure

1

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240

Helix Strand Turn

Details...

Helix

3 – 16

14

Helix

23 – 30

8

Helix

33 – 41

9

Helix

51 – 72

22

Helix

78 – 88

11

Helix

91 – 100

10

Helix

103 – 106

4

Helix

111 – 115

5

Helix

118 – 120

3

Helix

122 – 140

19

Helix

142 – 161

20

Helix

169 – 181

13

Beta strand

186 – 193

8

Beta strand

200 – 207

8

Beta strand

210 – 219

10

Helix

220 – 235

16

Sequences

	Sequence		Length	Mass (Da)
	Q9X0I6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 94330E8898D48A0D	FASTA	240	27,530
	10 20 30 40 50 60 MNESERKIVE EFQKETGINF KNEELLFRAL CHSSYANEQN QAGRKDVESN EKLEFLGDAV 70 80 90 100 110 120 LELFVCEILY KKYPEAEVGD LARVKSAAAS EEVLAMVSRK MNLGKFLFLG KGEEKTGGRD 130 140 150 160 170 180 RDSILADAFE ALLAAIYLDQ GYEKIKELFE QEFEFYIEKI MKGEMLFDYK TALQEIVQSE 190 200 210 220 230 240 HKVPPEYILV RTEKNDGDRI FVVEVRVNGK TIATGKGRTK KEAEKEAARI AYEKLLKERS

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000512 Genomic DNA. Translation: AAD36178.1.

PIR

H72294.

RefSeq

NP_228908.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O0W	X-ray	2.00	A/B	1-240	[»]

ModBase

Search...

Genome annotation databases

GeneID

898663.

GenomeReviews

Gene locus TM_1102 in contig AE000512_GR.

KEGG

tma:TM1102.

NMPDR

fig|243274.1.peg.1092.

TIGR

TM_1102.

Phylogenomic databases

HOGENOM

Q9X0I6.

OMA

ALTHKSF

Enzyme and pathway databases

BioCyc

TMAR243274:TM_1102-MON.

BRENDA

3.1.26.3. 16699.

Family and domain databases

HAMAP

MF_00104.
[Tree]

InterPro

IPR001159. Ds-RNA_bd.
IPR014720. dsRNA-bd-like.
IPR000999. RNase_III.
IPR011907. RNase_III_bac.
[Graphical view]

Gene3D

G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit.
G3DSA:1.10.1520.10. RNase_III. 1 hit.

Pfam

PF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]

SMART

SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02191. RNaseIII. 1 hit.

PROSITE

PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RNC_THEMA

Accession

Primary (citable) accession number: Q9X0I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)