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Protein

Adenylosuccinate lyase

Gene

purB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP.
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes AMP from IMP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA), Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (purB), Adenylosuccinate lyase (Tmari_1101)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC), Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC)
  2. Adenylosuccinate lyase (purB), Adenylosuccinate lyase (Tmari_1101)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95SubstrateBy similarity1
Active sitei141Proton acceptor1 Publication1
Active sitei141Proton donor/acceptorBy similarity1
Binding sitei212SubstrateBy similarity1
Active sitei262Proton donor/acceptorBy similarity1
Binding sitei301SubstrateBy similarity1
Binding sitei306SubstrateBy similarity1
Binding sitei310SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BRENDAi4.3.2.2. 6331.
UniPathwayiUPA00074; UER00132.
UPA00075; UER00336.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate lyase (EC:4.3.2.2)
Short name:
ASL
Alternative name(s):
Adenylosuccinase
Short name:
ASase
Gene namesi
Name:purB
Ordered Locus Names:TM_1095
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001378861 – 431Adenylosuccinate lyaseAdd BLAST431

Interactioni

Subunit structurei

Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.1 Publication

Protein-protein interaction databases

STRINGi243274.TM1095.

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi10 – 13Combined sources4
Helixi16 – 36Combined sources21
Helixi44 – 51Combined sources8
Helixi56 – 66Combined sources11
Helixi69 – 81Combined sources13
Helixi82 – 87Combined sources6
Turni88 – 91Combined sources4
Helixi94 – 130Combined sources37
Turni131 – 133Combined sources3
Beta strandi135 – 140Combined sources6
Beta strandi143 – 149Combined sources7
Helixi150 – 174Combined sources25
Beta strandi188 – 190Combined sources3
Helixi192 – 201Combined sources10
Beta strandi211 – 213Combined sources3
Helixi217 – 243Combined sources27
Turni246 – 248Combined sources3
Beta strandi250 – 252Combined sources3
Helixi272 – 286Combined sources15
Helixi288 – 293Combined sources6
Helixi304 – 333Combined sources30
Helixi338 – 345Combined sources8
Turni346 – 350Combined sources5
Helixi351 – 353Combined sources3
Helixi354 – 363Combined sources10
Helixi368 – 383Combined sources16
Beta strandi385 – 387Combined sources3
Helixi389 – 394Combined sources6
Helixi397 – 400Combined sources4
Helixi405 – 410Combined sources6
Helixi415 – 418Combined sources4
Helixi421 – 426Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C3CX-ray1.80A/B2-430[»]
1C3UX-ray2.30A/B1-431[»]
ProteinModelPortaliQ9X0I0.
SMRiQ9X0I0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X0I0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 5Substrate bindingBy similarity2
Regioni67 – 69Substrate bindingBy similarity3
Regioni68 – 69Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C83. Bacteria.
COG0015. LUCA.
InParanoidiQ9X0I0.
KOiK01756.
OMAiVQENAMK.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00928. purB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X0I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVERYSLSPM KDLWTEEAKY RRWLEVELAV TRAYEELGMI PKGVTERIRN
60 70 80 90 100
NAKIDVELFK KIEEKTNHDV VAFVEGIGSM IGEDSRFFHY GLTSSDVLDT
110 120 130 140 150
ANSLALVEAG KILLESLKEF CDVLWEVANR YKHTPTIGRT HGVHAEPTSF
160 170 180 190 200
GLKVLGWYSE MKRNVQRLER AIEEVSYGKI SGAVGNYANV PPEVEEKALS
210 220 230 240 250
YLGLKPEPVS TQVVPRDRHA FYLSTLAIVA AGIERIAVEI RHLQRTEVLE
260 270 280 290 300
VEEPFRKGQR GSSAMPHKKN PITCERLTGL SRMMRAYVDP SLENIALWHE
310 320 330 340 350
RDISHSSVER YVFPDATQTL YYMIVTATNV VRNMKVNEER MKKNIDLTKG
360 370 380 390 400
LVFSQRVLLK LIEKGLTRKE AYDIVQRNAL KTWNSEKHFL EYLLEDEEVK
410 420 430
KLVTKEELEE LFDISYYLKH VDHIFERFEK E
Length:431
Mass (Da):49,873
Last modified:November 1, 1999 - v1
Checksum:iD3ACB25D87582869
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36171.1.
PIRiA72294.
RefSeqiNP_228901.1. NC_000853.1.
WP_004080360.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36171; AAD36171; TM_1095.
GeneIDi898670.
KEGGitma:TM1095.
PATRICi23937125. VBITheMar51294_1111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD36171.1.
PIRiA72294.
RefSeqiNP_228901.1. NC_000853.1.
WP_004080360.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C3CX-ray1.80A/B2-430[»]
1C3UX-ray2.30A/B1-431[»]
ProteinModelPortaliQ9X0I0.
SMRiQ9X0I0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36171; AAD36171; TM_1095.
GeneIDi898670.
KEGGitma:TM1095.
PATRICi23937125. VBITheMar51294_1111.

Phylogenomic databases

eggNOGiENOG4105C83. Bacteria.
COG0015. LUCA.
InParanoidiQ9X0I0.
KOiK01756.
OMAiVQENAMK.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00132.
UPA00075; UER00336.
BRENDAi4.3.2.2. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9X0I0.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00928. purB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR8_THEMA
AccessioniPrimary (citable) accession number: Q9X0I0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.