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Q9X027 (TRMB_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:TM_0925
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171413

Regions

Region177 – 1804Substrate binding By similarity

Sites

Binding site331S-adenosyl-L-methionine By similarity
Binding site581S-adenosyl-L-methionine By similarity
Binding site851S-adenosyl-L-methionine By similarity
Binding site1121Substrate By similarity
Binding site1441Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9X027 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 34EE4D3669AD2CF3

FASTA31336,770
        10         20         30         40         50         60 
MVVTEYELKP QNFPRFPLDW SEIFGRKAKI VVEIGFGNGE FLAELARRHP EKDFVGFEVS 

        70         80         90        100        110        120 
ITSFVKAQKK FKRYNLKNVR LVKVDARFGL RELFPDNSVE KVYINFPCPW PKKRHESRRI 

       130        140        150        160        170        180 
TSYDFLQTLS AVLEMDGTVE FATDEEWYAR EVLDTFESSE YFVVDVFEEN FKRDVETRYE 

       190        200        210        220        230        240 
RKWKSQGKKT FLIVARKVKN GTVKRLMEGE NTMAHSVFEG NVTWEKLKEL EGKVFKDKNK 

       250        260        270        280        290        300 
IFVVKKVYRD GDYLLKVIST DEGGFQQVYY LNLSGRDGKW VLKLDEGSDP YRTPALKWSL 

       310 
RRIPEELTAQ GSP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD36006.1.
PIRF72317.
RefSeqNP_228733.1. NC_000853.1.
YP_007977277.1. NC_021214.1.
YP_008990105.1. NC_023151.1.

3D structure databases

ProteinModelPortalQ9X027.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0925.

Proteomic databases

PRIDEQ9X027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36006; AAD36006; TM_0925.
GeneID15494530.
18091859.
898599.
KEGGtma:TM0925.
tmm:Tmari_0927.
PATRIC23936781. VBITheMar51294_0939.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAFPCPWPK.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK871654.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_THEMA
AccessionPrimary (citable) accession number: Q9X027
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways