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Q9X005

- CHED_THEMA

UniProt

Q9X005 - CHED_THEMA

Protein

Chemoreceptor glutamine deamidase CheD

Gene

cheD

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Deamidates glutamine residues on chemoreceptors (MCPs). CheD-mediated MCP deamidation is required for productive communication of the conformational signals of the chemoreceptors to the CheA kinase. In addition, demethylates methylated glutamate residues on chemoreceptors Mcp2 and Mcp4. Enhances the activity of CheC.

    Catalytic activityi

    Protein L-glutamine + H2O = protein L-glutamate + NH3.
    Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei27 – 271Nucleophile

    GO - Molecular functioni

    1. protein-glutamate methylesterase activity Source: UniProtKB-EC
    2. protein-glutamine glutaminase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. chemotaxis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Chemotaxis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chemoreceptor glutamine deamidase CheD (EC:3.5.1.44)
    Alternative name(s):
    Chemoreceptor glutamate methylesterase CheD (EC:3.1.1.61)
    Gene namesi
    Name:cheD
    Ordered Locus Names:TM_0903
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi21 – 211T → A: 6-fold reduction in activity. 1 Publication
    Mutagenesisi26 – 261S → A or H: Loss of activity. 1 Publication
    Mutagenesisi26 – 261S → N: Reduced activity. 1 Publication
    Mutagenesisi27 – 271C → A: Loss of activity. Does not prevent binding to mcp2. 1 Publication
    Mutagenesisi27 – 271C → H or N: Loss of activity. 1 Publication
    Mutagenesisi44 – 441H → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Chemoreceptor glutamine deamidase CheDPRO_0000251072Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer with CheC. The CheC-CheD heterodimer interacts with phosphorylated CheY.1 Publication

    Protein-protein interaction databases

    STRINGi243274.TM0903.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 43
    Beta strandi10 – 145
    Beta strandi18 – 269
    Beta strandi28 – 347
    Turni35 – 384
    Beta strandi39 – 457
    Helixi57 – 593
    Helixi61 – 7313
    Turni74 – 763
    Helixi79 – 813
    Beta strandi83 – 886
    Helixi100 – 11415
    Beta strandi119 – 1246
    Beta strandi130 – 1356
    Turni136 – 1394
    Beta strandi140 – 1445
    Beta strandi154 – 1574

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2F9ZX-ray2.40C/D1-157[»]
    ProteinModelPortaliQ9X005.
    SMRiQ9X005. Positions 1-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X005.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CheD family.Curated

    Phylogenomic databases

    eggNOGiCOG1871.
    KOiK03411.
    OMAiIGMIHIM.
    OrthoDBiEOG6WHNQB.

    Family and domain databases

    HAMAPiMF_01440. CheD.
    InterProiIPR005659. Chemorcpt_Glu_NH3ase_CheD.
    IPR011324. Cytotoxic_necrot_fac-like_cat.
    [Graphical view]
    PfamiPF03975. CheD. 1 hit.
    [Graphical view]
    SUPFAMiSSF64438. SSF64438. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9X005-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKVIGIGEY AVMKNPGVIV TLGLGSCVAV CMRDPVAKVG AMAHVMLPDS    50
    GGKTDKPGKY ADTAVKTLVE ELKKMGAKVE RLEAKIAGGA SMFESKGMNI 100
    GARNVEAVKK HLKDFGIKLL AEDTGGNRAR SVEYNIETGK LLVRKVGGGE 150
    QLEIKEI 157
    Length:157
    Mass (Da):16,657
    Last modified:November 1, 1999 - v1
    Checksum:iE5B03D96D2DDEDBE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35984.1.
    PIRiF72318.
    RefSeqiNP_228711.1. NC_000853.1.
    YP_007977255.1. NC_021214.1.
    YP_008990127.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35984; AAD35984; TM_0903.
    GeneIDi898577.
    KEGGitma:TM0903.
    tmi:THEMA_00120.
    tmm:Tmari_0905.
    PATRICi23936737. VBITheMar51294_0917.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35984.1 .
    PIRi F72318.
    RefSeqi NP_228711.1. NC_000853.1.
    YP_007977255.1. NC_021214.1.
    YP_008990127.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2F9Z X-ray 2.40 C/D 1-157 [» ]
    ProteinModelPortali Q9X005.
    SMRi Q9X005. Positions 1-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0903.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35984 ; AAD35984 ; TM_0903 .
    GeneIDi 898577.
    KEGGi tma:TM0903.
    tmi:THEMA_00120.
    tmm:Tmari_0905.
    PATRICi 23936737. VBITheMar51294_0917.

    Phylogenomic databases

    eggNOGi COG1871.
    KOi K03411.
    OMAi IGMIHIM.
    OrthoDBi EOG6WHNQB.

    Miscellaneous databases

    EvolutionaryTracei Q9X005.

    Family and domain databases

    HAMAPi MF_01440. CheD.
    InterProi IPR005659. Chemorcpt_Glu_NH3ase_CheD.
    IPR011324. Cytotoxic_necrot_fac-like_cat.
    [Graphical view ]
    Pfami PF03975. CheD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64438. SSF64438. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "A receptor-modifying deamidase in complex with a signaling phosphatase reveals reciprocal regulation."
      Chao X., Muff T.J., Park S.-Y., Zhang S., Pollard A.M., Ordal G.W., Bilwes A.M., Crane B.R.
      Cell 124:561-571(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH CHEC, MUTAGENESIS OF THR-21; SER-26; CYS-27 AND HIS-44.

    Entry informationi

    Entry nameiCHED_THEMA
    AccessioniPrimary (citable) accession number: Q9X005
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3