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Protein

1-deoxy-D-xylulose 5-phosphate reductoisomerase

Gene

dxr

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171SubstrateUniRule annotation
Metal bindingi142 – 1421Divalent metal cationUniRule annotation
Metal bindingi144 – 1441Divalent metal cationUniRule annotation
Binding sitei144 – 1441SubstrateUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation
Binding sitei187 – 1871SubstrateUniRule annotation
Metal bindingi209 – 2091Divalent metal cationUniRule annotation
Binding sitei209 – 2091SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 3729NADPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.267. 6331.
UniPathwayiUPA00056; UER00092.

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomeraseUniRule annotation (EC:1.1.1.267UniRule annotation)
Short name:
DXP reductoisomeraseUniRule annotation
Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomeraseUniRule annotation
2-C-methyl-D-erythritol 4-phosphate synthaseUniRule annotation
Gene namesi
Name:dxrUniRule annotation
Ordered Locus Names:TM_0889
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3763761-deoxy-D-xylulose 5-phosphate reductoisomerasePRO_0000163723Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0889.

Structurei

Secondary structure

1
376
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Turni10 – 123Combined sources
Helixi14 – 2512Combined sources
Beta strandi28 – 3811Combined sources
Helixi40 – 5011Combined sources
Beta strandi54 – 574Combined sources
Beta strandi66 – 738Combined sources
Helixi76 – 849Combined sources
Beta strandi87 – 915Combined sources
Helixi98 – 10811Combined sources
Beta strandi110 – 1145Combined sources
Helixi118 – 13417Combined sources
Beta strandi137 – 1404Combined sources
Helixi143 – 1519Combined sources
Beta strandi157 – 1637Combined sources
Beta strandi169 – 1713Combined sources
Helixi173 – 1764Combined sources
Helixi181 – 1833Combined sources
Helixi194 – 2029Combined sources
Helixi204 – 21714Combined sources
Helixi221 – 2233Combined sources
Beta strandi224 – 2285Combined sources
Beta strandi234 – 2396Combined sources
Beta strandi245 – 2495Combined sources
Helixi255 – 2639Combined sources
Beta strandi278 – 2803Combined sources
Turni287 – 2893Combined sources
Helixi293 – 2953Combined sources
Helixi296 – 2994Combined sources
Helixi303 – 32119Combined sources
Helixi329 – 33911Combined sources
Turni340 – 3434Combined sources
Helixi350 – 37122Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A06X-ray2.00A/B1-376[»]
3A14X-ray2.00A/B1-376[»]
ProteinModelPortaliQ9WZZ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZZ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the DXR family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CEA. Bacteria.
COG0743. LUCA.
InParanoidiQ9WZZ1.
KOiK00099.
OMAiHKYNVKI.
OrthoDBiEOG6R2H04.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERTLVILG ATGSIGTQTL DVLKKVKGIR LIGISFHSNL ELAFKIVKEF
60 70 80 90 100
NVKNVAITGD VEFEDSSINV WKGSHSIEEM LEALKPDITM VAVSGFSGLR
110 120 130 140 150
AVLASLEHSK RVCLANKESL VCGGFLVKKK LKEKGTELIP VDSEHSAIFQ
160 170 180 190 200
VMEPEVEKVV LTASGGALRD WKISKIDRAR PEDVLKHPVW NMGARITVDS
210 220 230 240 250
ATMVNKAFEV LEAMELFELP FEKIEVKIHR EGLVHGAVVL PDGNVKMVVS
260 270 280 290 300
PPDMRIPISY ALFYPRRVAL EPFFLRTISL SFEDPDPEKY PAFFLLKEIK
310 320 330 340 350
DSYALRTAFN AADEVAVEAF LKGRIRFGGI HRVIEKTLEE FQGYPQPRTL
360 370
DDVERIHFEA IKKAERVTEW LSSTSY
Length:376
Mass (Da):42,200
Last modified:November 1, 1999 - v1
Checksum:iC0D3B68DB0C4BF57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35970.1.
PIRiB72321.
RefSeqiNP_228697.1. NC_000853.1.
WP_004080699.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35970; AAD35970; TM_0889.
GeneIDi898563.
KEGGitma:TM0889.
PATRICi23936709. VBITheMar51294_0903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35970.1.
PIRiB72321.
RefSeqiNP_228697.1. NC_000853.1.
WP_004080699.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A06X-ray2.00A/B1-376[»]
3A14X-ray2.00A/B1-376[»]
ProteinModelPortaliQ9WZZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35970; AAD35970; TM_0889.
GeneIDi898563.
KEGGitma:TM0889.
PATRICi23936709. VBITheMar51294_0903.

Phylogenomic databases

eggNOGiENOG4105CEA. Bacteria.
COG0743. LUCA.
InParanoidiQ9WZZ1.
KOiK00099.
OMAiHKYNVKI.
OrthoDBiEOG6R2H04.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00092.
BRENDAi1.1.1.267. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZZ1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiDXR_THEMA
AccessioniPrimary (citable) accession number: Q9WZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.