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Protein

1-deoxy-D-xylulose 5-phosphate reductoisomerase

Gene

dxr

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117SubstrateUniRule annotation1
Metal bindingi142Divalent metal cationUniRule annotation1
Metal bindingi144Divalent metal cationUniRule annotation1
Binding sitei144SubstrateUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Binding sitei187SubstrateUniRule annotation1
Metal bindingi209Divalent metal cationUniRule annotation1
Binding sitei209SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 37NADPUniRule annotationAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.267. 6331.
UniPathwayiUPA00056; UER00092.

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomeraseUniRule annotation (EC:1.1.1.267UniRule annotation)
Short name:
DXP reductoisomeraseUniRule annotation
Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomeraseUniRule annotation
2-C-methyl-D-erythritol 4-phosphate synthaseUniRule annotation
Gene namesi
Name:dxrUniRule annotation
Ordered Locus Names:TM_0889
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001637231 – 3761-deoxy-D-xylulose 5-phosphate reductoisomeraseAdd BLAST376

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0889.

Structurei

Secondary structure

1376
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Turni10 – 12Combined sources3
Helixi14 – 25Combined sources12
Beta strandi28 – 38Combined sources11
Helixi40 – 50Combined sources11
Beta strandi54 – 57Combined sources4
Beta strandi66 – 73Combined sources8
Helixi76 – 84Combined sources9
Beta strandi87 – 91Combined sources5
Helixi98 – 108Combined sources11
Beta strandi110 – 114Combined sources5
Helixi118 – 134Combined sources17
Beta strandi137 – 140Combined sources4
Helixi143 – 151Combined sources9
Beta strandi157 – 163Combined sources7
Beta strandi169 – 171Combined sources3
Helixi173 – 176Combined sources4
Helixi181 – 183Combined sources3
Helixi194 – 202Combined sources9
Helixi204 – 217Combined sources14
Helixi221 – 223Combined sources3
Beta strandi224 – 228Combined sources5
Beta strandi234 – 239Combined sources6
Beta strandi245 – 249Combined sources5
Helixi255 – 263Combined sources9
Beta strandi278 – 280Combined sources3
Turni287 – 289Combined sources3
Helixi293 – 295Combined sources3
Helixi296 – 299Combined sources4
Helixi303 – 321Combined sources19
Helixi329 – 339Combined sources11
Turni340 – 343Combined sources4
Helixi350 – 371Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A06X-ray2.00A/B1-376[»]
3A14X-ray2.00A/B1-376[»]
ProteinModelPortaliQ9WZZ1.
SMRiQ9WZZ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZZ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the DXR family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CEA. Bacteria.
COG0743. LUCA.
InParanoidiQ9WZZ1.
KOiK00099.
OMAiGFCPLSE.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom. 1 hit.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERTLVILG ATGSIGTQTL DVLKKVKGIR LIGISFHSNL ELAFKIVKEF
60 70 80 90 100
NVKNVAITGD VEFEDSSINV WKGSHSIEEM LEALKPDITM VAVSGFSGLR
110 120 130 140 150
AVLASLEHSK RVCLANKESL VCGGFLVKKK LKEKGTELIP VDSEHSAIFQ
160 170 180 190 200
VMEPEVEKVV LTASGGALRD WKISKIDRAR PEDVLKHPVW NMGARITVDS
210 220 230 240 250
ATMVNKAFEV LEAMELFELP FEKIEVKIHR EGLVHGAVVL PDGNVKMVVS
260 270 280 290 300
PPDMRIPISY ALFYPRRVAL EPFFLRTISL SFEDPDPEKY PAFFLLKEIK
310 320 330 340 350
DSYALRTAFN AADEVAVEAF LKGRIRFGGI HRVIEKTLEE FQGYPQPRTL
360 370
DDVERIHFEA IKKAERVTEW LSSTSY
Length:376
Mass (Da):42,200
Last modified:November 1, 1999 - v1
Checksum:iC0D3B68DB0C4BF57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35970.1.
PIRiB72321.
RefSeqiNP_228697.1. NC_000853.1.
WP_004080699.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35970; AAD35970; TM_0889.
GeneIDi898563.
KEGGitma:TM0889.
PATRICi23936709. VBITheMar51294_0903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35970.1.
PIRiB72321.
RefSeqiNP_228697.1. NC_000853.1.
WP_004080699.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A06X-ray2.00A/B1-376[»]
3A14X-ray2.00A/B1-376[»]
ProteinModelPortaliQ9WZZ1.
SMRiQ9WZZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35970; AAD35970; TM_0889.
GeneIDi898563.
KEGGitma:TM0889.
PATRICi23936709. VBITheMar51294_0903.

Phylogenomic databases

eggNOGiENOG4105CEA. Bacteria.
COG0743. LUCA.
InParanoidiQ9WZZ1.
KOiK00099.
OMAiGFCPLSE.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00092.
BRENDAi1.1.1.267. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZZ1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom. 1 hit.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDXR_THEMA
AccessioniPrimary (citable) accession number: Q9WZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.