Q9WZW1 (Q9WZW1_THEMA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Riboflavin biosynthesis protein RibF EMBL EHA61202.1

Submitted name:
Riboflavin kinase/FMN adenylyltransferase EMBL AAD35939.1

Gene names

Ordered Locus Names:	TM_0857
ORF Names:	ThemaDRAFT_0445 EMBL EHA61202.1

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	FMN PDB 1T6Y Flavoprotein PDB 1T6Y Nucleotide-binding PDB 1T6Y PDB 1T6X
Molecular function	Kinase EMBL AAD35939.1 Nucleotidyltransferase EMBL AAD35939.1 Transferase
Technical term	3D-structure PDB 1T6Y PDB 1T6X PDB 1T6Z PDB 1S4M PDB 1MRZ PDB 2I1L Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	FMN adenylyltransferase activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	7 – 8	2	AMP PDB 1T6Y
	Nucleotide binding	12 – 15	4	AMP PDB 1T6Y
	Nucleotide binding	99 – 101	3	AMP PDB 1T6Y
	Nucleotide binding	127 – 128	2	AMP PDB 1T6Y
	Nucleotide binding	179 – 182	4	ADP PDB 1T6Y PDB 1T6X
	Nucleotide binding	231 – 242	12	ADP PDB 1T6Y PDB 1T6X
	Nucleotide binding	255 – 258	4	FMN PDB 1T6Y
Sites
	Binding site	135	1	AMP; via carbonyl oxygen PDB 1T6Y
	Binding site	215	1	FMN PDB 1T6Y

Sequences

	Sequence		Length	Mass (Da)
	Q9WZW1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 42B56B582C03BEB4	FASTA	293	33,614
	10 20 30 40 50 60 MVVSIGVFDG VHIGHQKVLR TMKEIAFFRK DDSLIYTISY PPEYFLPDFP GLLMTVESRV 70 80 90 100 110 120 EMLSRYARTV VLDFFRIKDL TPEGFVERYL SGVSAVVVGR DFRFGKNASG NASFLRKKGV 130 140 150 160 170 180 EVYEIEDVVV QGKRVSSSLI RNLVQEGRVE EIPAYLGRYF EIEGIVHKDR EFGRKLGFPT 190 200 210 220 230 240 ANIDRGNEKL VDLKRGVYLV RVHLPDGKKK FGVMNVGFRP TVGDARNVKY EVYILDFEGD 250 260 270 280 290 LYGQRLKLEV LKFMRDEKKF DSIEELKAAI DQDVKSARNM IDDIINSKFE KEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Crystal structure of a flavin-binding protein from Thermotoga maritima." Wang W., Kim R., Jancarik J., Yokota H., Kim S.H. Proteins 52:633-635(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).
[3]	"Crystal structure of ADP bound FAD synthetase." Shin D.H., Wang W., Kim R., Yokota H., Kim S.-H. Submitted (MAY-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH ADP; AMP AND FMN.
[4]	"Crystal structure of flavin binding to FAD synthetase of Thermotoga maritima." Wang W., Kim R., Yokota H., Kim S.H. Proteins 58:246-248(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).
[5]	"Crystal structure of the C2 form of FAD synthetase from Thermotoga maritima." Wang W., Shin D.H., Yokota H., Kim R., Kim S.-H. Submitted (AUG-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).
[6]	"The draft genome of Thermotoga maritima MSB8." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Land M.L., Hauser L., Boucher N., Noll K., Woyke T. Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: MSB8 EMBL EHA61202.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000512 Genomic DNA. Translation: AAD35939.1.
AGIJ01000001 Genomic DNA. Translation: EHA61202.1.

PIR

B72325.

RefSeq

NP_228666.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MRZ	X-ray	1.90	A/B	1-293	[»]
1S4M	X-ray	2.10	A/B	1-293	[»]
1T6X	X-ray	2.29	A/B	1-293	[»]
1T6Y	X-ray	2.80	A/B	1-293	[»]
1T6Z	X-ray	2.40	A/B	1-293	[»]
2I1L	X-ray	2.50	A/B	1-293	[»]

SMR

Q9WZW1. Positions 1-288.

ModBase

Search...

Protein-protein interaction databases

STRING

243274.TM0857.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD35939; AAD35939; TM_0857.
EHA61202; EHA61202; ThemaDRAFT_0445.

GeneID

898530.

KEGG

tma:TM0857.

PATRIC

23936642. VBITheMar51294_0870.

Phylogenomic databases

KO

K11753.

OMA

FHFGRGR.

ProtClustDB

CLSK875429.

Family and domain databases

Gene3D

2.40.30.30. 1 hit.
3.40.50.620. 1 hit.

InterPro

IPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

PANTHER

PTHR22749. PTHR22749. 1 hit.

Pfam

PF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
[Graphical view]

PIRSF

PIRSF004491. FAD_Synth. 1 hit.

SMART

SM00904. Flavokinase. 1 hit.
[Graphical view]

SUPFAM

SSF82114. Riboflavin_kinase. 1 hit.

TIGRFAMs

TIGR00083. ribF. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9WZW1.

Entry information

Entry name

Q9WZW1_THEMA

Accession

Primary (citable) accession number: Q9WZW1
Secondary accession number(s): G4FCW7

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1999
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)