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Q9WZW0 (TRUB_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase B

EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:TM_0856
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309tRNA pseudouridine synthase B HAMAP-Rule MF_01080
PRO_0000121929

Regions

Domain229 – 30678PUA

Sites

Active site391Nucleophile

Secondary structure

................................................................ 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9WZW0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 04825FD6D643CE9C

FASTA30935,459
        10         20         30         40         50         60 
MKHGILVAYK PKGPTSHDVV DEVRKKLKTR KVGHGGTLDP FACGVLIIGV NQGTRILEFY 

        70         80         90        100        110        120 
KDLKKVYWVK MRLGLITETF DITGEVVEER ECNVTEEEIR EAIFSFVGEY DQVPPAYSAK 

       130        140        150        160        170        180 
KYKGERLYKL AREGKIINLP PKRVKIFKIW DVNIEGRDVS FRVEVSPGTY IRSLCMDIGY 

       190        200        210        220        230        240 
KLGCGATAVE LVRESVGPHT IEESLNVFEA APEEIENRII PLEKCLEWLP RVVVHQESTK 

       250        260        270        280        290        300 
MILNGSQIHL EMLKEWDGFK KGEVVRVFNE EGRLLALAEA ERNSSFLETL RKHERNERVL 


TLRKVFNTR 

« Hide

References

« Hide 'large scale' references
[1]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Preliminary X-ray crystallographic analysis of tRNA pseudouridine 55 synthase from the thermophilic eubacterium Thermotoga maritima."
Wouters J., Tricot C., Durbecq V., Roovers M., Stalon V., Droogmans L.
Acta Crystallogr. D 59:152-154(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]"Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit."
Pan H., Agarwalla S., Moustakas D.T., Finer-Moore J., Stroud R.M.
Proc. Natl. Acad. Sci. U.S.A. 100:12648-12653(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF COMPLEX WITH RNA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD35938.1.
PIRA72325.
RefSeqNP_228665.1. NC_000853.1.
YP_007977208.1. NC_021214.1.
YP_008990174.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1R3EX-ray2.10A1-309[»]
1ZE1X-ray2.90A/B/C/D1-309[»]
1ZE2X-ray3.00A/B1-309[»]
2AB4X-ray2.40A1-309[»]
ProteinModelPortalQ9WZW0.
SMRQ9WZW0. Positions 1-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0856.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35938; AAD35938; TM_0856.
GeneID15494461.
18091927.
898529.
KEGGtma:TM0856.
tmm:Tmari_0858.
PATRIC23936640. VBITheMar51294_0869.

Phylogenomic databases

eggNOGCOG0130.
KOK03177.
OMAIPITHAL.
OrthoDBEOG6358D3.
ProtClustDBPRK14124.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR00431. TruB. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9WZW0.

Entry information

Entry nameTRUB_THEMA
AccessionPrimary (citable) accession number: Q9WZW0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1999
Last modified: March 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references