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Q9WZW0

- TRUB_THEMA

UniProt

Q9WZW0 - TRUB_THEMA

Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.By similarity

    Catalytic activityi

    tRNA uridine(55) = tRNA pseudouridine(55).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei39 – 391Nucleophile

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: UniProtKB-HAMAP
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine synthase B (EC:5.4.99.25)
    Alternative name(s):
    tRNA pseudouridine(55) synthase
    Short name:
    Psi55 synthase
    tRNA pseudouridylate synthase
    tRNA-uridine isomerase
    Gene namesi
    Name:truB
    Ordered Locus Names:TM_0856
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309tRNA pseudouridine synthase BPRO_0000121929Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243274.TM0856.

    Structurei

    Secondary structure

    1
    309
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 107
    Beta strandi12 – 143
    Helixi16 – 2611
    Beta strandi32 – 365
    Beta strandi43 – 508
    Helixi51 – 6010
    Beta strandi65 – 739
    Beta strandi75 – 817
    Beta strandi86 – 894
    Helixi96 – 1049
    Beta strandi105 – 1128
    Beta strandi116 – 1194
    Beta strandi121 – 1266
    Helixi127 – 1326
    Beta strandi137 – 1393
    Beta strandi142 – 15514
    Beta strandi158 – 1658
    Helixi171 – 18111
    Beta strandi186 – 19611
    Helixi201 – 2033
    Turni207 – 2093
    Helixi212 – 2187
    Helixi222 – 2243
    Beta strandi231 – 2333
    Turni236 – 2383
    Helixi239 – 2424
    Turni243 – 2453
    Beta strandi248 – 2525
    Beta strandi253 – 2553
    Beta strandi264 – 2685
    Beta strandi270 – 2723
    Beta strandi274 – 2829
    Helixi287 – 2904
    Beta strandi291 – 2944
    Beta strandi298 – 3058

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1R3EX-ray2.10A1-309[»]
    1ZE1X-ray2.90A/B/C/D1-309[»]
    1ZE2X-ray3.00A/B1-309[»]
    2AB4X-ray2.40A1-309[»]
    ProteinModelPortaliQ9WZW0.
    SMRiQ9WZW0. Positions 1-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WZW0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini229 – 30678PUAAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PUA domain.Curated

    Phylogenomic databases

    eggNOGiCOG0130.
    KOiK03177.
    OMAiSAAWHNG.
    OrthoDBiEOG6358D3.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    HAMAPiMF_01080. TruB_bact.
    InterProiIPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    IPR014780. tRNA_psdUridine_synth_TruB.
    [Graphical view]
    PANTHERiPTHR13767. PTHR13767. 1 hit.
    PfamiPF01472. PUA. 1 hit.
    PF01509. TruB_N. 1 hit.
    [Graphical view]
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR00431. TruB. 1 hit.
    PROSITEiPS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WZW0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKHGILVAYK PKGPTSHDVV DEVRKKLKTR KVGHGGTLDP FACGVLIIGV    50
    NQGTRILEFY KDLKKVYWVK MRLGLITETF DITGEVVEER ECNVTEEEIR 100
    EAIFSFVGEY DQVPPAYSAK KYKGERLYKL AREGKIINLP PKRVKIFKIW 150
    DVNIEGRDVS FRVEVSPGTY IRSLCMDIGY KLGCGATAVE LVRESVGPHT 200
    IEESLNVFEA APEEIENRII PLEKCLEWLP RVVVHQESTK MILNGSQIHL 250
    EMLKEWDGFK KGEVVRVFNE EGRLLALAEA ERNSSFLETL RKHERNERVL 300
    TLRKVFNTR 309
    Length:309
    Mass (Da):35,459
    Last modified:November 1, 1999 - v1
    Checksum:i04825FD6D643CE9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35938.1.
    PIRiA72325.
    RefSeqiNP_228665.1. NC_000853.1.
    YP_007977208.1. NC_021214.1.
    YP_008990174.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35938; AAD35938; TM_0856.
    GeneIDi898529.
    KEGGitma:TM0856.
    tmi:THEMA_00355.
    tmm:Tmari_0858.
    PATRICi23936640. VBITheMar51294_0869.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35938.1 .
    PIRi A72325.
    RefSeqi NP_228665.1. NC_000853.1.
    YP_007977208.1. NC_021214.1.
    YP_008990174.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1R3E X-ray 2.10 A 1-309 [» ]
    1ZE1 X-ray 2.90 A/B/C/D 1-309 [» ]
    1ZE2 X-ray 3.00 A/B 1-309 [» ]
    2AB4 X-ray 2.40 A 1-309 [» ]
    ProteinModelPortali Q9WZW0.
    SMRi Q9WZW0. Positions 1-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0856.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35938 ; AAD35938 ; TM_0856 .
    GeneIDi 898529.
    KEGGi tma:TM0856.
    tmi:THEMA_00355.
    tmm:Tmari_0858.
    PATRICi 23936640. VBITheMar51294_0869.

    Phylogenomic databases

    eggNOGi COG0130.
    KOi K03177.
    OMAi SAAWHNG.
    OrthoDBi EOG6358D3.

    Miscellaneous databases

    EvolutionaryTracei Q9WZW0.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    HAMAPi MF_01080. TruB_bact.
    InterProi IPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    IPR014780. tRNA_psdUridine_synth_TruB.
    [Graphical view ]
    PANTHERi PTHR13767. PTHR13767. 1 hit.
    Pfami PF01472. PUA. 1 hit.
    PF01509. TruB_N. 1 hit.
    [Graphical view ]
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR00431. TruB. 1 hit.
    PROSITEi PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Preliminary X-ray crystallographic analysis of tRNA pseudouridine 55 synthase from the thermophilic eubacterium Thermotoga maritima."
      Wouters J., Tricot C., Durbecq V., Roovers M., Stalon V., Droogmans L.
      Acta Crystallogr. D 59:152-154(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit."
      Pan H., Agarwalla S., Moustakas D.T., Finer-Moore J., Stroud R.M.
      Proc. Natl. Acad. Sci. U.S.A. 100:12648-12653(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF COMPLEX WITH RNA.

    Entry informationi

    Entry nameiTRUB_THEMA
    AccessioniPrimary (citable) accession number: Q9WZW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3