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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.By similarity

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei39 – 391Nucleophile

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BRENDAi5.4.99.25. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase B (EC:5.4.99.25)
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene namesi
Name:truB
Ordered Locus Names:TM_0856
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309tRNA pseudouridine synthase BPRO_0000121929Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0856.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Beta strandi12 – 143Combined sources
Helixi16 – 2611Combined sources
Beta strandi32 – 365Combined sources
Beta strandi43 – 508Combined sources
Helixi51 – 6010Combined sources
Beta strandi65 – 739Combined sources
Beta strandi75 – 817Combined sources
Beta strandi86 – 894Combined sources
Helixi96 – 1049Combined sources
Beta strandi105 – 1128Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi121 – 1266Combined sources
Helixi127 – 1326Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi142 – 15514Combined sources
Beta strandi158 – 1658Combined sources
Helixi171 – 18111Combined sources
Beta strandi186 – 19611Combined sources
Helixi201 – 2033Combined sources
Turni207 – 2093Combined sources
Helixi212 – 2187Combined sources
Helixi222 – 2243Combined sources
Beta strandi231 – 2333Combined sources
Turni236 – 2383Combined sources
Helixi239 – 2424Combined sources
Turni243 – 2453Combined sources
Beta strandi248 – 2525Combined sources
Beta strandi253 – 2553Combined sources
Beta strandi264 – 2685Combined sources
Beta strandi270 – 2723Combined sources
Beta strandi274 – 2829Combined sources
Helixi287 – 2904Combined sources
Beta strandi291 – 2944Combined sources
Beta strandi298 – 3058Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R3EX-ray2.10A1-309[»]
1ZE1X-ray2.90A/B/C/D1-309[»]
1ZE2X-ray3.00A/B1-309[»]
2AB4X-ray2.40A1-309[»]
ProteinModelPortaliQ9WZW0.
SMRiQ9WZW0. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZW0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini229 – 30678PUAAdd
BLAST

Sequence similaritiesi

Contains 1 PUA domain.Curated

Phylogenomic databases

eggNOGiCOG0130.
InParanoidiQ9WZW0.
KOiK03177.
OMAiHATKISG.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WZW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHGILVAYK PKGPTSHDVV DEVRKKLKTR KVGHGGTLDP FACGVLIIGV
60 70 80 90 100
NQGTRILEFY KDLKKVYWVK MRLGLITETF DITGEVVEER ECNVTEEEIR
110 120 130 140 150
EAIFSFVGEY DQVPPAYSAK KYKGERLYKL AREGKIINLP PKRVKIFKIW
160 170 180 190 200
DVNIEGRDVS FRVEVSPGTY IRSLCMDIGY KLGCGATAVE LVRESVGPHT
210 220 230 240 250
IEESLNVFEA APEEIENRII PLEKCLEWLP RVVVHQESTK MILNGSQIHL
260 270 280 290 300
EMLKEWDGFK KGEVVRVFNE EGRLLALAEA ERNSSFLETL RKHERNERVL

TLRKVFNTR
Length:309
Mass (Da):35,459
Last modified:November 1, 1999 - v1
Checksum:i04825FD6D643CE9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35938.1.
PIRiA72325.
RefSeqiNP_228665.1. NC_000853.1.
WP_004080766.1. NC_023151.1.
YP_007977208.1. NC_021214.1.
YP_008990174.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35938; AAD35938; TM_0856.
GeneIDi898529.
KEGGitma:TM0856.
tmi:THEMA_00355.
tmm:Tmari_0858.
PATRICi23936640. VBITheMar51294_0869.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35938.1.
PIRiA72325.
RefSeqiNP_228665.1. NC_000853.1.
WP_004080766.1. NC_023151.1.
YP_007977208.1. NC_021214.1.
YP_008990174.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R3EX-ray2.10A1-309[»]
1ZE1X-ray2.90A/B/C/D1-309[»]
1ZE2X-ray3.00A/B1-309[»]
2AB4X-ray2.40A1-309[»]
ProteinModelPortaliQ9WZW0.
SMRiQ9WZW0. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35938; AAD35938; TM_0856.
GeneIDi898529.
KEGGitma:TM0856.
tmi:THEMA_00355.
tmm:Tmari_0858.
PATRICi23936640. VBITheMar51294_0869.

Phylogenomic databases

eggNOGiCOG0130.
InParanoidiQ9WZW0.
KOiK03177.
OMAiHATKISG.
OrthoDBiEOG6358D3.

Enzyme and pathway databases

BRENDAi5.4.99.25. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZW0.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Preliminary X-ray crystallographic analysis of tRNA pseudouridine 55 synthase from the thermophilic eubacterium Thermotoga maritima."
    Wouters J., Tricot C., Durbecq V., Roovers M., Stalon V., Droogmans L.
    Acta Crystallogr. D 59:152-154(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit."
    Pan H., Agarwalla S., Moustakas D.T., Finer-Moore J., Stroud R.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:12648-12653(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF COMPLEX WITH RNA.

Entry informationi

Entry nameiTRUB_THEMA
AccessioniPrimary (citable) accession number: Q9WZW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1999
Last modified: May 27, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.