Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei39NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BRENDAi5.4.99.25. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthase1 PublicationUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truB1 PublicationUniRule annotation
Ordered Locus Names:TM_0856
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219291 – 309tRNA pseudouridine synthase BAdd BLAST309

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0856.

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi12 – 14Combined sources3
Helixi16 – 26Combined sources11
Beta strandi32 – 36Combined sources5
Beta strandi43 – 50Combined sources8
Helixi51 – 60Combined sources10
Beta strandi65 – 73Combined sources9
Beta strandi75 – 81Combined sources7
Beta strandi86 – 89Combined sources4
Helixi96 – 104Combined sources9
Beta strandi105 – 112Combined sources8
Beta strandi116 – 119Combined sources4
Beta strandi121 – 126Combined sources6
Helixi127 – 132Combined sources6
Beta strandi137 – 139Combined sources3
Beta strandi142 – 155Combined sources14
Beta strandi158 – 165Combined sources8
Helixi171 – 181Combined sources11
Beta strandi186 – 196Combined sources11
Helixi201 – 203Combined sources3
Turni207 – 209Combined sources3
Helixi212 – 218Combined sources7
Helixi222 – 224Combined sources3
Beta strandi231 – 233Combined sources3
Turni236 – 238Combined sources3
Helixi239 – 242Combined sources4
Turni243 – 245Combined sources3
Beta strandi248 – 252Combined sources5
Beta strandi253 – 255Combined sources3
Beta strandi264 – 268Combined sources5
Beta strandi270 – 272Combined sources3
Beta strandi274 – 282Combined sources9
Helixi287 – 290Combined sources4
Beta strandi291 – 294Combined sources4
Beta strandi298 – 305Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R3EX-ray2.10A1-309[»]
1ZE1X-ray2.90A/B/C/D1-309[»]
1ZE2X-ray3.00A/B1-309[»]
2AB4X-ray2.40A1-309[»]
ProteinModelPortaliQ9WZW0.
SMRiQ9WZW0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZW0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini229 – 306PUAUniRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0T. Bacteria.
COG0130. LUCA.
InParanoidiQ9WZW0.
KOiK03177.
OMAiGIHELDY.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WZW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHGILVAYK PKGPTSHDVV DEVRKKLKTR KVGHGGTLDP FACGVLIIGV
60 70 80 90 100
NQGTRILEFY KDLKKVYWVK MRLGLITETF DITGEVVEER ECNVTEEEIR
110 120 130 140 150
EAIFSFVGEY DQVPPAYSAK KYKGERLYKL AREGKIINLP PKRVKIFKIW
160 170 180 190 200
DVNIEGRDVS FRVEVSPGTY IRSLCMDIGY KLGCGATAVE LVRESVGPHT
210 220 230 240 250
IEESLNVFEA APEEIENRII PLEKCLEWLP RVVVHQESTK MILNGSQIHL
260 270 280 290 300
EMLKEWDGFK KGEVVRVFNE EGRLLALAEA ERNSSFLETL RKHERNERVL

TLRKVFNTR
Length:309
Mass (Da):35,459
Last modified:November 1, 1999 - v1
Checksum:i04825FD6D643CE9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35938.1.
PIRiA72325.
RefSeqiNP_228665.1. NC_000853.1.
WP_004080766.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35938; AAD35938; TM_0856.
GeneIDi898529.
KEGGitma:TM0856.
PATRICi23936640. VBITheMar51294_0869.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35938.1.
PIRiA72325.
RefSeqiNP_228665.1. NC_000853.1.
WP_004080766.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R3EX-ray2.10A1-309[»]
1ZE1X-ray2.90A/B/C/D1-309[»]
1ZE2X-ray3.00A/B1-309[»]
2AB4X-ray2.40A1-309[»]
ProteinModelPortaliQ9WZW0.
SMRiQ9WZW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35938; AAD35938; TM_0856.
GeneIDi898529.
KEGGitma:TM0856.
PATRICi23936640. VBITheMar51294_0869.

Phylogenomic databases

eggNOGiENOG4105D0T. Bacteria.
COG0130. LUCA.
InParanoidiQ9WZW0.
KOiK03177.
OMAiGIHELDY.

Enzyme and pathway databases

BRENDAi5.4.99.25. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZW0.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRUB_THEMA
AccessioniPrimary (citable) accession number: Q9WZW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.