Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9WZW0

- TRUB_THEMA

UniProt

Q9WZW0 - TRUB_THEMA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.By similarity

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei39 – 391Nucleophile

GO - Molecular functioni

  1. pseudouridine synthase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: InterPro

GO - Biological processi

  1. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase B (EC:5.4.99.25)
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene namesi
Name:truB
Ordered Locus Names:TM_0856
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309tRNA pseudouridine synthase BPRO_0000121929Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0856.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Beta strandi12 – 143Combined sources
Helixi16 – 2611Combined sources
Beta strandi32 – 365Combined sources
Beta strandi43 – 508Combined sources
Helixi51 – 6010Combined sources
Beta strandi65 – 739Combined sources
Beta strandi75 – 817Combined sources
Beta strandi86 – 894Combined sources
Helixi96 – 1049Combined sources
Beta strandi105 – 1128Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi121 – 1266Combined sources
Helixi127 – 1326Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi142 – 15514Combined sources
Beta strandi158 – 1658Combined sources
Helixi171 – 18111Combined sources
Beta strandi186 – 19611Combined sources
Helixi201 – 2033Combined sources
Turni207 – 2093Combined sources
Helixi212 – 2187Combined sources
Helixi222 – 2243Combined sources
Beta strandi231 – 2333Combined sources
Turni236 – 2383Combined sources
Helixi239 – 2424Combined sources
Turni243 – 2453Combined sources
Beta strandi248 – 2525Combined sources
Beta strandi253 – 2553Combined sources
Beta strandi264 – 2685Combined sources
Beta strandi270 – 2723Combined sources
Beta strandi274 – 2829Combined sources
Helixi287 – 2904Combined sources
Beta strandi291 – 2944Combined sources
Beta strandi298 – 3058Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R3EX-ray2.10A1-309[»]
1ZE1X-ray2.90A/B/C/D1-309[»]
1ZE2X-ray3.00A/B1-309[»]
2AB4X-ray2.40A1-309[»]
ProteinModelPortaliQ9WZW0.
SMRiQ9WZW0. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZW0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini229 – 30678PUAAdd
BLAST

Sequence similaritiesi

Contains 1 PUA domain.Curated

Phylogenomic databases

eggNOGiCOG0130.
InParanoidiQ9WZW0.
KOiK03177.
OMAiSAAWHNG.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WZW0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHGILVAYK PKGPTSHDVV DEVRKKLKTR KVGHGGTLDP FACGVLIIGV
60 70 80 90 100
NQGTRILEFY KDLKKVYWVK MRLGLITETF DITGEVVEER ECNVTEEEIR
110 120 130 140 150
EAIFSFVGEY DQVPPAYSAK KYKGERLYKL AREGKIINLP PKRVKIFKIW
160 170 180 190 200
DVNIEGRDVS FRVEVSPGTY IRSLCMDIGY KLGCGATAVE LVRESVGPHT
210 220 230 240 250
IEESLNVFEA APEEIENRII PLEKCLEWLP RVVVHQESTK MILNGSQIHL
260 270 280 290 300
EMLKEWDGFK KGEVVRVFNE EGRLLALAEA ERNSSFLETL RKHERNERVL

TLRKVFNTR
Length:309
Mass (Da):35,459
Last modified:November 1, 1999 - v1
Checksum:i04825FD6D643CE9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35938.1.
PIRiA72325.
RefSeqiNP_228665.1. NC_000853.1.
YP_007977208.1. NC_021214.1.
YP_008990174.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35938; AAD35938; TM_0856.
GeneIDi18091927.
898529.
KEGGitma:TM0856.
PATRICi23936640. VBITheMar51294_0869.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35938.1 .
PIRi A72325.
RefSeqi NP_228665.1. NC_000853.1.
YP_007977208.1. NC_021214.1.
YP_008990174.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1R3E X-ray 2.10 A 1-309 [» ]
1ZE1 X-ray 2.90 A/B/C/D 1-309 [» ]
1ZE2 X-ray 3.00 A/B 1-309 [» ]
2AB4 X-ray 2.40 A 1-309 [» ]
ProteinModelPortali Q9WZW0.
SMRi Q9WZW0. Positions 1-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM0856.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD35938 ; AAD35938 ; TM_0856 .
GeneIDi 18091927.
898529.
KEGGi tma:TM0856.
PATRICi 23936640. VBITheMar51294_0869.

Phylogenomic databases

eggNOGi COG0130.
InParanoidi Q9WZW0.
KOi K03177.
OMAi SAAWHNG.
OrthoDBi EOG6358D3.

Miscellaneous databases

EvolutionaryTracei Q9WZW0.

Family and domain databases

Gene3Di 2.30.130.10. 1 hit.
HAMAPi MF_01080. TruB_bact.
InterProi IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view ]
PANTHERi PTHR13767. PTHR13767. 1 hit.
Pfami PF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view ]
SMARTi SM00359. PUA. 1 hit.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR00431. TruB. 1 hit.
PROSITEi PS50890. PUA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Preliminary X-ray crystallographic analysis of tRNA pseudouridine 55 synthase from the thermophilic eubacterium Thermotoga maritima."
    Wouters J., Tricot C., Durbecq V., Roovers M., Stalon V., Droogmans L.
    Acta Crystallogr. D 59:152-154(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit."
    Pan H., Agarwalla S., Moustakas D.T., Finer-Moore J., Stroud R.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:12648-12653(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF COMPLEX WITH RNA.

Entry informationi

Entry nameiTRUB_THEMA
AccessioniPrimary (citable) accession number: Q9WZW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3