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Protein

Bifunctional thiamine biosynthesis protein ThiDN

Gene

thiDN

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.By similarity
Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).1 Publication

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.1 Publication
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.1 Publication
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.1 Publication

Pathwayi

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401Hydroxymethylpyrimidine/phosphomethylpyrimidineBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: UniProtKB-EC
  3. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC
  4. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-817-MONOMER.
UniPathwayiUPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional thiamine biosynthesis protein ThiDN
Including the following 2 domains:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Thiamine-phosphate synthase ThiN (EC:2.5.1.3)
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Short name:
TMP-PPase
Gene namesi
Name:thiDN
Ordered Locus Names:TM_0790
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Bifunctional thiamine biosynthesis protein ThiDNPRO_0000415381Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0790.

Structurei

3D structure databases

ProteinModelPortaliQ9WZP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 216216Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseAdd
BLAST
Regioni217 – 398182Thiamine-phosphate synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the ThiN family.Curated

Phylogenomic databases

InParanoidiQ9WZP7.
KOiK00941.
OMAiEGATIPW.
OrthoDBiEOG6HMXB7.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.225.10. 1 hit.
InterProiIPR001303. Aldolase_II/adducin_N.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR019293. ThiN.
[Graphical view]
PfamiPF10120. Aldolase_2. 1 hit.
PF08543. Phos_pyr_kin. 2 hits.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
SSF53639. SSF53639. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZP7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLVVAGFDP SGGAGIIQDV KVLSALGVKT HAVISALTVQ NENRVFSVNF
60 70 80 90 100
RDWEEMRKEI EVLTPPRVIK VGLSAPETVK RLREMFPDSA IVWNVVLESS
110 120 130 140 150
SGFGFQDPEE VKKFVEYADY VILNSEEAKK LGEYNNFIVT GGHEKGNTVK
160 170 180 190 200
VKYRDFVFEI PRVPGEFHGT GCAFSSAVSG FLAMSYPVEE AIRSAMELLK
210 220 230 240 250
KILERSSGVV ETEKLLRDWY RYDTLNTLDE ILPEFLEIGH LTVPEVGQNV
260 270 280 290 300
SYALPWAKNE FEVGKFPGRI RLKEGKAVAV SCASFKDRSH TARMAVTMMR
310 320 330 340 350
YHPHMRCVVN VRYEREYVER AKKRGLKVFH YDRSKEPKEV QEKEGQSMVW
360 370 380 390
MIEQAIAELK SPPDVIYDEG WWGKEAMIRV FGRNPKEVLE KIKLMVRE
Length:398
Mass (Da):45,392
Last modified:November 1, 1999 - v1
Checksum:iDCE6977F471BF649
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35872.1.
PIRiG72333.
RefSeqiNP_228599.1. NC_000853.1.
YP_007977141.1. NC_021214.1.
YP_008990240.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35872; AAD35872; TM_0790.
GeneIDi18091996.
898458.
KEGGitma:TM0790.
tmi:THEMA_00700.
PATRICi23936500. VBITheMar51294_0802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35872.1.
PIRiG72333.
RefSeqiNP_228599.1. NC_000853.1.
YP_007977141.1. NC_021214.1.
YP_008990240.1. NC_023151.1.

3D structure databases

ProteinModelPortaliQ9WZP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0790.

Protocols and materials databases

DNASUi898458.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35872; AAD35872; TM_0790.
GeneIDi18091996.
898458.
KEGGitma:TM0790.
tmi:THEMA_00700.
PATRICi23936500. VBITheMar51294_0802.

Phylogenomic databases

InParanoidiQ9WZP7.
KOiK00941.
OMAiEGATIPW.
OrthoDBiEOG6HMXB7.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00141.
BioCyciTMAR243274:GC6P-817-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.225.10. 1 hit.
InterProiIPR001303. Aldolase_II/adducin_N.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR019293. ThiN.
[Graphical view]
PfamiPF10120. Aldolase_2. 1 hit.
PF08543. Phos_pyr_kin. 2 hits.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
SSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: FUNCTION AS A THIAMINE-PHOSPHATE SYNTHASE VIA ITS C-TERMINAL DOMAIN, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiTHIDN_THEMA
AccessioniPrimary (citable) accession number: Q9WZP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 1, 1999
Last modified: February 4, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.