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Q9WZP7

- THIDN_THEMA

UniProt

Q9WZP7 - THIDN_THEMA

Protein

Bifunctional thiamine biosynthesis protein ThiDN

Gene

thiDN

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.By similarity
    Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).1 Publication

    Catalytic activityi

    ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.1 Publication
    ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.1 Publication
    2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.1 Publication

    Pathwayi

    Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401Hydroxymethylpyrimidine/phosphomethylpyrimidineBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydroxymethylpyrimidine kinase activity Source: UniProtKB-EC
    3. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC
    4. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-817-MONOMER.
    UniPathwayiUPA00060; UER00141.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional thiamine biosynthesis protein ThiDN
    Including the following 2 domains:
    Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
    Alternative name(s):
    Hydroxymethylpyrimidine kinase
    Short name:
    HMP kinase
    Hydroxymethylpyrimidine phosphate kinase
    Short name:
    HMP-P kinase
    Short name:
    HMP-phosphate kinase
    Short name:
    HMPP kinase
    Thiamine-phosphate synthase ThiN (EC:2.5.1.3)
    Short name:
    TP synthase
    Short name:
    TPS
    Alternative name(s):
    Thiamine-phosphate pyrophosphorylase
    Short name:
    TMP pyrophosphorylase
    Short name:
    TMP-PPase
    Gene namesi
    Name:thiDN
    Ordered Locus Names:TM_0790
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Bifunctional thiamine biosynthesis protein ThiDNPRO_0000415381Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243274.TM0790.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WZP7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 216216Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseAdd
    BLAST
    Regioni217 – 398182Thiamine-phosphate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the ThiD family.Curated
    In the C-terminal section; belongs to the ThiN family.Curated

    Phylogenomic databases

    KOiK00941.
    OMAiEGATIPW.
    OrthoDBiEOG6HMXB7.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    3.40.225.10. 1 hit.
    InterProiIPR001303. Aldolase_II/adducin_N.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    IPR019293. ThiN.
    [Graphical view]
    PfamiPF10120. Aldolase_2. 1 hit.
    PF08543. Phos_pyr_kin. 2 hits.
    [Graphical view]
    SUPFAMiSSF53613. SSF53613. 1 hit.
    SSF53639. SSF53639. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WZP7-1 [UniParc]FASTAAdd to Basket

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    MVLVVAGFDP SGGAGIIQDV KVLSALGVKT HAVISALTVQ NENRVFSVNF    50
    RDWEEMRKEI EVLTPPRVIK VGLSAPETVK RLREMFPDSA IVWNVVLESS 100
    SGFGFQDPEE VKKFVEYADY VILNSEEAKK LGEYNNFIVT GGHEKGNTVK 150
    VKYRDFVFEI PRVPGEFHGT GCAFSSAVSG FLAMSYPVEE AIRSAMELLK 200
    KILERSSGVV ETEKLLRDWY RYDTLNTLDE ILPEFLEIGH LTVPEVGQNV 250
    SYALPWAKNE FEVGKFPGRI RLKEGKAVAV SCASFKDRSH TARMAVTMMR 300
    YHPHMRCVVN VRYEREYVER AKKRGLKVFH YDRSKEPKEV QEKEGQSMVW 350
    MIEQAIAELK SPPDVIYDEG WWGKEAMIRV FGRNPKEVLE KIKLMVRE 398
    Length:398
    Mass (Da):45,392
    Last modified:November 1, 1999 - v1
    Checksum:iDCE6977F471BF649
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35872.1.
    PIRiG72333.
    RefSeqiNP_228599.1. NC_000853.1.
    YP_007977141.1. NC_021214.1.
    YP_008990240.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35872; AAD35872; TM_0790.
    GeneIDi898458.
    KEGGitma:TM0790.
    tmi:THEMA_00700.
    tmm:Tmari_0791.
    PATRICi23936500. VBITheMar51294_0802.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35872.1 .
    PIRi G72333.
    RefSeqi NP_228599.1. NC_000853.1.
    YP_007977141.1. NC_021214.1.
    YP_008990240.1. NC_023151.1.

    3D structure databases

    ProteinModelPortali Q9WZP7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0790.

    Protocols and materials databases

    DNASUi 898458.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35872 ; AAD35872 ; TM_0790 .
    GeneIDi 898458.
    KEGGi tma:TM0790.
    tmi:THEMA_00700.
    tmm:Tmari_0791.
    PATRICi 23936500. VBITheMar51294_0802.

    Phylogenomic databases

    KOi K00941.
    OMAi EGATIPW.
    OrthoDBi EOG6HMXB7.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00141 .
    BioCyci TMAR243274:GC6P-817-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    3.40.225.10. 1 hit.
    InterProi IPR001303. Aldolase_II/adducin_N.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    IPR019293. ThiN.
    [Graphical view ]
    Pfami PF10120. Aldolase_2. 1 hit.
    PF08543. Phos_pyr_kin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53613. SSF53613. 1 hit.
    SSF53639. SSF53639. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. Cited for: FUNCTION AS A THIAMINE-PHOSPHATE SYNTHASE VIA ITS C-TERMINAL DOMAIN, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiTHIDN_THEMA
    AccessioniPrimary (citable) accession number: Q9WZP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3