Q9WZN3 (IF2_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2

Gene names

Name:	infB
Ordered Locus Names:	TM_0775

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100_B
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Translation initiation factor IF-2 HAMAP-Rule MF_00100_B		PRO_0000137272
Regions
	Nucleotide binding	187 – 194	8	GTP By similarity
	Nucleotide binding	233 – 237	5	GTP By similarity
	Nucleotide binding	287 – 290	4	GTP By similarity
	Region	181 – 330	150	G-domain HAMAP-Rule MF_00100_B
	Compositional bias	79 – 84	6	Poly-Lys HAMAP-Rule MF_00100_B

Sequences

	Sequence		Length	Mass (Da)
	Q9WZN3 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8673FFFA20C37B60	FASTA	690	77,774
	10 20 30 40 50 60 MARLRVYELA RKLNMSPKEL LQELEELGVN VKSHMSYVDE EMANIIIDLL EEDNRKAKQP 70 80 90 100 110 120 SKPKKEKGEE EVEKEVVEKK KKKKITLKPD ELKLDIIAEK IGVPQNKIIQ DMFVKRGIAL 130 140 150 160 170 180 RPGQILKLEE VEQILKEYKI EIEIEEEQQT SVEEVDEFEL LEKRYQELYE KEKDKLVPRP 190 200 210 220 230 240 PVVTVMGHVD HGKTTLLDRI RSTRVAEREE GGITQSIGAY QVEVNGKKIT FIDTPGHELF 250 260 270 280 290 300 TEMRARGAQA TDIVVLVVAA DDGVMPQTIE AYNHAKAANV PIIVAINKID KPNANVEKTK 310 320 330 340 350 360 QELVEKLGLI PEEWGGDTIV VPISARTGQG VDELLEMILL VAEMNEIKCY PEGPARAVII 370 380 390 400 410 420 ESKLDKKMGP VASAIVKDGV LKVGDAVVAS NTYGRVRNLF DDNMRPIREA YPSQPVMILG 430 440 450 460 470 480 FEDVPDVHSN VYVVESAEKA KEIVEKRLQR LEAQKQSRKH INLEELMKMM QEKEKKVLNL 490 500 510 520 530 540 ILKADTYGSV AALKNAINKL QSKEIELNIV HAGVGEISTS DVMLAAAVDG VILGFRVKVN 550 560 570 580 590 600 NQARRLAEQE GVDVRTYSII YKLVEDLKLA LEGMLEPEEV EEVIGHGEIR KVFKISKVGK 610 620 630 640 650 660 VAGVQMLDGK ADRNGFVRIY RNGQLVFGGK IESLKHYKED VSVVEAPQEC GIKFAGFDDI 670 680 690 HEGDELEFYV IRKVKRKPTF VEEQADQEQK

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD35857.1.
PIR	E72337.
RefSeq	NP_228584.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9WZN3.
ModBase	Search...
Protein-protein interaction databases
STRING	243274.TM0775.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD35857; AAD35857; TM_0775.
GeneID	898443.
KEGG	tma:TM0775.
PATRIC	23936472. VBITheMar51294_0788.
Phylogenomic databases
eggNOG	COG0532.
KO	K02519.
OMA	HIGAFTV.
ProtClustDB	PRK05306.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF-2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 2 hits. [Graphical view]
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2_THEMA

Accession

Primary (citable) accession number: Q9WZN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program