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Protein

Phosphopantetheine adenylyltransferase

Gene

coaD

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.UniRule annotation

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes CoA from (R)-pantothenate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Type III pantothenate kinase (coaX), Type III pantothenate kinase (coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (TM_1687), Coenzyme A biosynthesis bifunctional protein CoaBC (Tmari_1695)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (TM_1687), Coenzyme A biosynthesis bifunctional protein CoaBC (Tmari_1695)
  4. Phosphopantetheine adenylyltransferase (coaD), Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE), Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei16ATPUniRule annotation1
Sitei16Transition state stabilizer1
Binding sitei72Substrate; via amide nitrogenUniRule annotationCombined sources1 Publication1
Binding sitei86SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei97ATPUniRule annotation1
Binding sitei132Substrate; shared with a neighboring subunitCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 9ATPUniRule annotation2
Nucleotide bindingi87 – 89ATPUniRule annotation3
Nucleotide bindingi122 – 128ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCoenzyme A biosynthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00241; UER00355.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferaseUniRule annotation (EC:2.7.7.3UniRule annotation)
Alternative name(s):
Dephospho-CoA pyrophosphorylaseUniRule annotation
Pantetheine-phosphate adenylyltransferaseUniRule annotation
Short name:
PPATUniRule annotation
Gene namesi
Name:coaDUniRule annotation
Synonyms:kdtB
Ordered Locus Names:TM_0741
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB03912. 4'-Phosphopantetheine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001562961 – 161Phosphopantetheine adenylyltransferaseAdd BLAST161

Interactioni

Subunit structurei

Homohexamer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243274.TM0741.

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi14 – 24Combined sources11
Beta strandi28 – 35Combined sources8
Helixi46 – 56Combined sources11
Turni57 – 59Combined sources3
Beta strandi63 – 68Combined sources6
Helixi72 – 79Combined sources8
Beta strandi83 – 88Combined sources6
Helixi94 – 107Combined sources14
Beta strandi112 – 117Combined sources6
Helixi120 – 122Combined sources3
Helixi127 – 135Combined sources9
Turni141 – 143Combined sources3
Helixi146 – 155Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VLHX-ray2.20A/B/C/D/E/F1-161[»]
ProteinModelPortaliQ9WZK0.
SMRiQ9WZK0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZK0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 40Substrate bindingCombined sources1 Publication5

Sequence similaritiesi

Belongs to the bacterial CoaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZEF. Bacteria.
COG0669. LUCA.
InParanoidiQ9WZK0.
KOiK00954.
OMAiEFQMALM.

Family and domain databases

CDDicd02163. PPAT. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact. 1 hit.
InterProiView protein in InterPro
IPR004821. Cyt_trans-like.
IPR001980. PPAT.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR21342:SF3. PTHR21342:SF3. 1 hit.
PfamiView protein in Pfam
PF01467. CTP_transf_like. 1 hit.
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVYPGSFD PITLGHVDII KRALSIFDEL VVLVTENPRK KCMFTLEERK
60 70 80 90 100
KLIEEVLSDL DGVKVDVHHG LLVDYLKKHG IKVLVRGLRA VTDYEYELQM
110 120 130 140 150
ALANKKLYSD LETVFLIASE KFSFISSSLV KEVALYGGDV TEWVPPEVAR
160
ALNEKLKEGK R
Length:161
Mass (Da):18,249
Last modified:November 1, 1999 - v1
Checksum:i4A0F62B9D368496F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35822.1.
PIRiH72339.
RefSeqiNP_228550.1. NC_000853.1.
WP_004080981.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35822; AAD35822; TM_0741.
GeneIDi898408.
KEGGitma:TM0741.

Similar proteinsi

Entry informationi

Entry nameiCOAD_THEMA
AccessioniPrimary (citable) accession number: Q9WZK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: September 27, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families