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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.UniRule annotation

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP.UniRule annotation

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp), Uracil phosphoribosyltransferase (Tmari_0722)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 7915-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei104 – 10415-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei194 – 1941Uracil; via amide nitrogenUniRule annotation
Binding sitei200 – 20015-phospho-alpha-D-ribose 1-diphosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferaseUniRule annotation (EC:2.4.2.9UniRule annotation)
Alternative name(s):
UMP pyrophosphorylaseUniRule annotation
UPRTaseUniRule annotation
Gene namesi
Name:uppUniRule annotation
Ordered Locus Names:TM_0721
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Uracil phosphoribosyltransferasePRO_0000120904Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0721.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Helixi10 – 2011Combined sources
Helixi26 – 4419Combined sources
Turni45 – 473Combined sources
Beta strandi51 – 566Combined sources
Beta strandi61 – 666Combined sources
Beta strandi72 – 787Combined sources
Helixi81 – 9111Combined sources
Beta strandi99 – 1046Combined sources
Turni106 – 1083Combined sources
Beta strandi111 – 1177Combined sources
Beta strandi126 – 1305Combined sources
Beta strandi132 – 1376Combined sources
Helixi138 – 14912Combined sources
Beta strandi154 – 1585Combined sources
Beta strandi160 – 1623Combined sources
Helixi164 – 17310Combined sources
Beta strandi178 – 1847Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi194 – 1974Combined sources
Helixi201 – 2066Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O5OX-ray2.30A/B/C/D1-209[»]
ProteinModelPortaliQ9WZI0.
SMRiQ9WZI0. Positions 1-209.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZI0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 13995-phospho-alpha-D-ribose 1-diphosphate bindingUniRule annotation
Regioni199 – 2013Uracil bindingUniRule annotation

Sequence similaritiesi

Belongs to the UPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
InParanoidiQ9WZI0.
KOiK00761.
OMAiTIEGWCG.
OrthoDBiEOG6HF5WX.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B.
InterProiIPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNLVVVDHP LIKHKLTIMR DKNTGPKEFR ELLREITLLL AYEATRHLKC
60 70 80 90 100
EEVEVETPIT KTIGYRINDK DIVVVPILRA GLVMADGILE LLPNASVGHI
110 120 130 140 150
GIYRDPETLQ AVEYYAKLPP LNDDKEVFLL DPMLATGVSS IKAIEILKEN
160 170 180 190 200
GAKKITLVAL IAAPEGVEAV EKKYEDVKIY VAALDERLND HGYIIPGLGD

AGDRLFRTK
Length:209
Mass (Da):23,320
Last modified:November 1, 1999 - v1
Checksum:iBD306120FF64D586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35803.1.
PIRiG72341.
RefSeqiNP_228530.1. NC_000853.1.
WP_004081012.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35803; AAD35803; TM_0721.
GeneIDi898388.
KEGGitma:TM0721.
PATRICi23936362. VBITheMar51294_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35803.1.
PIRiG72341.
RefSeqiNP_228530.1. NC_000853.1.
WP_004081012.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O5OX-ray2.30A/B/C/D1-209[»]
ProteinModelPortaliQ9WZI0.
SMRiQ9WZI0. Positions 1-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35803; AAD35803; TM_0721.
GeneIDi898388.
KEGGitma:TM0721.
PATRICi23936362. VBITheMar51294_0734.

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
InParanoidiQ9WZI0.
KOiK00761.
OMAiTIEGWCG.
OrthoDBiEOG6HF5WX.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.

Miscellaneous databases

EvolutionaryTraceiQ9WZI0.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B.
InterProiIPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Crystal structure of uracil phosphoribosyltransferase (TM0721) from Thermotoga maritima at 2.30 A resolution."
    Joint center for structural genomics (JCSG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiUPP_THEMA
AccessioniPrimary (citable) accession number: Q9WZI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.