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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.UniRule annotation

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP.UniRule annotation

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp), Uracil phosphoribosyltransferase (upp)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei795-phospho-alpha-D-ribose 1-diphosphateUniRule annotation1
Binding sitei1045-phospho-alpha-D-ribose 1-diphosphateUniRule annotation1
Binding sitei194Uracil; via amide nitrogenUniRule annotation1
Binding sitei2005-phospho-alpha-D-ribose 1-diphosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferaseUniRule annotation (EC:2.4.2.9UniRule annotation)
Alternative name(s):
UMP pyrophosphorylaseUniRule annotation
UPRTaseUniRule annotation
Gene namesi
Name:uppUniRule annotation
Ordered Locus Names:TM_0721
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209041 – 209Uracil phosphoribosyltransferaseAdd BLAST209

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0721.

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi10 – 20Combined sources11
Helixi26 – 44Combined sources19
Turni45 – 47Combined sources3
Beta strandi51 – 56Combined sources6
Beta strandi61 – 66Combined sources6
Beta strandi72 – 78Combined sources7
Helixi81 – 91Combined sources11
Beta strandi99 – 104Combined sources6
Turni106 – 108Combined sources3
Beta strandi111 – 117Combined sources7
Beta strandi126 – 130Combined sources5
Beta strandi132 – 137Combined sources6
Helixi138 – 149Combined sources12
Beta strandi154 – 158Combined sources5
Beta strandi160 – 162Combined sources3
Helixi164 – 173Combined sources10
Beta strandi178 – 184Combined sources7
Beta strandi186 – 188Combined sources3
Beta strandi194 – 197Combined sources4
Helixi201 – 206Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O5OX-ray2.30A/B/C/D1-209[»]
ProteinModelPortaliQ9WZI0.
SMRiQ9WZI0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZI0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 1395-phospho-alpha-D-ribose 1-diphosphate bindingUniRule annotation9
Regioni199 – 201Uracil bindingUniRule annotation3

Sequence similaritiesi

Belongs to the UPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
InParanoidiQ9WZI0.
KOiK00761.
OMAiVTVIDHP.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNLVVVDHP LIKHKLTIMR DKNTGPKEFR ELLREITLLL AYEATRHLKC
60 70 80 90 100
EEVEVETPIT KTIGYRINDK DIVVVPILRA GLVMADGILE LLPNASVGHI
110 120 130 140 150
GIYRDPETLQ AVEYYAKLPP LNDDKEVFLL DPMLATGVSS IKAIEILKEN
160 170 180 190 200
GAKKITLVAL IAAPEGVEAV EKKYEDVKIY VAALDERLND HGYIIPGLGD

AGDRLFRTK
Length:209
Mass (Da):23,320
Last modified:November 1, 1999 - v1
Checksum:iBD306120FF64D586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35803.1.
PIRiG72341.
RefSeqiNP_228530.1. NC_000853.1.
WP_004081012.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35803; AAD35803; TM_0721.
GeneIDi898388.
KEGGitma:TM0721.
PATRICi23936362. VBITheMar51294_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35803.1.
PIRiG72341.
RefSeqiNP_228530.1. NC_000853.1.
WP_004081012.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O5OX-ray2.30A/B/C/D1-209[»]
ProteinModelPortaliQ9WZI0.
SMRiQ9WZI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35803; AAD35803; TM_0721.
GeneIDi898388.
KEGGitma:TM0721.
PATRICi23936362. VBITheMar51294_0734.

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
InParanoidiQ9WZI0.
KOiK00761.
OMAiVTVIDHP.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.

Miscellaneous databases

EvolutionaryTraceiQ9WZI0.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUPP_THEMA
AccessioniPrimary (citable) accession number: Q9WZI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.