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Protein

Flagellar motor switch protein FliM

Gene

fliM

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheX chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar motor switch protein FliMImported
Gene namesi
Name:fliMBy similarity
Ordered Locus Names:TM_0679
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60E → C: No appreciable effect on the binding affinity for CheY. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004212401 – 328Flagellar motor switch protein FliMAdd BLAST328

Interactioni

Subunit structurei

Interacts (via N-terminus) with unphosphorylated CheY. Interacts (via central domain) with FliG (via central domain or via central domain and C-terminus).3 Publications

Protein-protein interaction databases

DIPiDIP-61248N.
IntActiQ9WZE6. 1 interactor.
MINTiMINT-8212457.
STRINGi243274.TM0679.

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 16Combined sources10
Helixi49 – 73Combined sources25
Beta strandi79 – 88Combined sources10
Helixi89 – 95Combined sources7
Beta strandi101 – 107Combined sources7
Beta strandi114 – 118Combined sources5
Helixi120 – 130Combined sources11
Helixi145 – 165Combined sources21
Turni166 – 169Combined sources4
Beta strandi174 – 181Combined sources8
Helixi183 – 185Combined sources3
Beta strandi193 – 204Combined sources12
Beta strandi207 – 216Combined sources10
Helixi217 – 220Combined sources4
Helixi221 – 223Combined sources3
Turni228 – 232Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HP7X-ray2.00A44-226[»]
3SOHX-ray3.50A/C46-233[»]
4FHRX-ray1.93A46-230[»]
4IGAX-ray1.73B1-20[»]
4QRMX-ray4.32A/C/E/G/I/K/M/O/Q/S/U46-228[»]
ProteinModelPortaliQ9WZE6.
SMRiQ9WZE6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZE6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 45Interaction with unphosphorylated CheY1 PublicationAdd BLAST45

Sequence similaritiesi

Belongs to the FliM family.Sequence analysis

Phylogenomic databases

eggNOGiENOG4105D24. Bacteria.
COG1868. LUCA.
InParanoidiQ9WZE6.
KOiK02416.
OMAiNPQFTQI.

Family and domain databases

Gene3Di3.40.1550.10. 1 hit.
InterProiIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamiPF02154. FliM. 1 hit.
PF01052. FliMN_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002888. FliM. 1 hit.
PRINTSiPR00955. FLGMOTORFLIM.
SUPFAMiSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsiTIGR01397. fliM_switch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDVLSQEEI NQLIEALMKG ELKEEDLLKE EEEKKVKPYD FKRPSKFSKE
60 70 80 90 100
QLRTFQMIHE NFGRALSTYL SGRLRTFVDV EISIDQLTYE EFIRSVMIPS
110 120 130 140 150
FIVIFTGDVF EGSAIFEMRL DLFYTMLDII MGGPGENPPN RPPTEIETSI
160 170 180 190 200
MRKEVTNMLT LLAQAWSDFQ YFIPSIENVE TNPQFVQIVP PNEIVLLVTA
210 220 230 240 250
SVSWGEFTSF INVCWPFSLL EPLLEKLSDR FWMMGRKPEK VEERMEELRK
260 270 280 290 300
ASQKIPVTVQ AVIGETELRL KEILDLEVGD VIRLGTHYKD EIRIDVEGRP
310 320
KFRGIPGVFK GKYAVKVTGE FTNGGEYE
Length:328
Mass (Da):37,888
Last modified:November 1, 1999 - v1
Checksum:i3E122C1FFAEBB093
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35762.1.
PIRiE72347.
RefSeqiNP_228487.1. NC_000853.1.
WP_004081085.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35762; AAD35762; TM_0679.
GeneIDi898347.
KEGGitma:TM0679.
PATRICi23936274. VBITheMar51294_0690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35762.1.
PIRiE72347.
RefSeqiNP_228487.1. NC_000853.1.
WP_004081085.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HP7X-ray2.00A44-226[»]
3SOHX-ray3.50A/C46-233[»]
4FHRX-ray1.93A46-230[»]
4IGAX-ray1.73B1-20[»]
4QRMX-ray4.32A/C/E/G/I/K/M/O/Q/S/U46-228[»]
ProteinModelPortaliQ9WZE6.
SMRiQ9WZE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61248N.
IntActiQ9WZE6. 1 interactor.
MINTiMINT-8212457.
STRINGi243274.TM0679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35762; AAD35762; TM_0679.
GeneIDi898347.
KEGGitma:TM0679.
PATRICi23936274. VBITheMar51294_0690.

Phylogenomic databases

eggNOGiENOG4105D24. Bacteria.
COG1868. LUCA.
InParanoidiQ9WZE6.
KOiK02416.
OMAiNPQFTQI.

Miscellaneous databases

EvolutionaryTraceiQ9WZE6.

Family and domain databases

Gene3Di3.40.1550.10. 1 hit.
InterProiIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamiPF02154. FliM. 1 hit.
PF01052. FliMN_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002888. FliM. 1 hit.
PRINTSiPR00955. FLGMOTORFLIM.
SUPFAMiSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsiTIGR01397. fliM_switch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFLIM_THEMA
AccessioniPrimary (citable) accession number: Q9WZE6
Secondary accession number(s): G4FDE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.