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Q9WZE6

- FLIM_THEMA

UniProt

Q9WZE6 - FLIM_THEMA

Protein

Flagellar motor switch protein FliM

Gene

fliM

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheX chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation By similarity.By similarity

    GO - Molecular functioni

    1. motor activity Source: InterPro
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. bacterial-type flagellum-dependent cell motility Source: InterPro
    2. chemotaxis Source: UniProtKB-KW

    Keywords - Biological processi

    Chemotaxis, Flagellar rotation

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-704-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flagellar motor switch protein FliMImported
    Gene namesi
    Name:fliMBy similarity
    Ordered Locus Names:TM_0679
    ORF Names:ThemaDRAFT_0623
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cell inner membrane By similarity; Peripheral membrane protein By similarity. Bacterial flagellum basal body By similarity

    GO - Cellular componenti

    1. bacterial-type flagellum basal body Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Bacterial flagellum, Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi60 – 601E → C: No appreciable effect on the binding affinity for CheY. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Flagellar motor switch protein FliMPRO_0000421240Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts (via N-terminus) with unphosphorylated CheY. Interacts (via central domain) with FliG (via central domain or via central domain and C-terminus).3 Publications

    Protein-protein interaction databases

    IntActiQ9WZE6. 1 interaction.
    MINTiMINT-8212457.
    STRINGi243274.TM0679.

    Structurei

    Secondary structure

    1
    328
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1610
    Helixi49 – 7325
    Beta strandi79 – 8810
    Helixi89 – 957
    Beta strandi101 – 1077
    Beta strandi114 – 1185
    Helixi120 – 13011
    Helixi145 – 16521
    Turni166 – 1694
    Beta strandi174 – 1818
    Helixi183 – 1853
    Beta strandi193 – 20412
    Beta strandi207 – 21610
    Helixi217 – 2204
    Helixi221 – 2233
    Turni228 – 2325

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HP7X-ray2.00A44-226[»]
    3SOHX-ray3.50A/C46-233[»]
    4FHRX-ray1.93A46-230[»]
    4IGAX-ray1.73B1-20[»]
    ProteinModelPortaliQ9WZE6.
    SMRiQ9WZE6. Positions 44-226.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WZE6.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4545Interaction with unphosphorylated CheY1 PublicationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the FliM family.Sequence Analysis

    Phylogenomic databases

    KOiK02416.
    OMAiLFNMMRR.
    OrthoDBiEOG68M4DH.

    Family and domain databases

    InterProiIPR028976. CheC-like_dom.
    IPR001689. Flag_FliM.
    IPR001543. SpoA.
    [Graphical view]
    PfamiPF02154. FliM. 1 hit.
    PF01052. SpoA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002888. FliM. 1 hit.
    PRINTSiPR00955. FLGMOTORFLIM.
    SUPFAMiSSF101801. SSF101801. 1 hit.
    SSF103039. SSF103039. 1 hit.
    TIGRFAMsiTIGR01397. fliM_switch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WZE6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDVLSQEEI NQLIEALMKG ELKEEDLLKE EEEKKVKPYD FKRPSKFSKE    50
    QLRTFQMIHE NFGRALSTYL SGRLRTFVDV EISIDQLTYE EFIRSVMIPS 100
    FIVIFTGDVF EGSAIFEMRL DLFYTMLDII MGGPGENPPN RPPTEIETSI 150
    MRKEVTNMLT LLAQAWSDFQ YFIPSIENVE TNPQFVQIVP PNEIVLLVTA 200
    SVSWGEFTSF INVCWPFSLL EPLLEKLSDR FWMMGRKPEK VEERMEELRK 250
    ASQKIPVTVQ AVIGETELRL KEILDLEVGD VIRLGTHYKD EIRIDVEGRP 300
    KFRGIPGVFK GKYAVKVTGE FTNGGEYE 328
    Length:328
    Mass (Da):37,888
    Last modified:November 1, 1999 - v1
    Checksum:i3E122C1FFAEBB093
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35762.1.
    AGIJ01000001 Genomic DNA. Translation: EHA61380.1.
    PIRiE72347.
    RefSeqiNP_228487.1. NC_000853.1.
    YP_007977029.1. NC_021214.1.
    YP_008990352.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35762; AAD35762; TM_0679.
    GeneIDi898347.
    KEGGitma:TM0679.
    tmi:THEMA_01270.
    tmm:Tmari_0679.
    PATRICi23936274. VBITheMar51294_0690.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35762.1 .
    AGIJ01000001 Genomic DNA. Translation: EHA61380.1 .
    PIRi E72347.
    RefSeqi NP_228487.1. NC_000853.1.
    YP_007977029.1. NC_021214.1.
    YP_008990352.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HP7 X-ray 2.00 A 44-226 [» ]
    3SOH X-ray 3.50 A/C 46-233 [» ]
    4FHR X-ray 1.93 A 46-230 [» ]
    4IGA X-ray 1.73 B 1-20 [» ]
    ProteinModelPortali Q9WZE6.
    SMRi Q9WZE6. Positions 44-226.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WZE6. 1 interaction.
    MINTi MINT-8212457.
    STRINGi 243274.TM0679.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35762 ; AAD35762 ; TM_0679 .
    GeneIDi 898347.
    KEGGi tma:TM0679.
    tmi:THEMA_01270.
    tmm:Tmari_0679.
    PATRICi 23936274. VBITheMar51294_0690.

    Phylogenomic databases

    KOi K02416.
    OMAi LFNMMRR.
    OrthoDBi EOG68M4DH.

    Enzyme and pathway databases

    BioCyci TMAR243274:GC6P-704-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9WZE6.

    Family and domain databases

    InterProi IPR028976. CheC-like_dom.
    IPR001689. Flag_FliM.
    IPR001543. SpoA.
    [Graphical view ]
    Pfami PF02154. FliM. 1 hit.
    PF01052. SpoA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002888. FliM. 1 hit.
    PRINTSi PR00955. FLGMOTORFLIM.
    SUPFAMi SSF101801. SSF101801. 1 hit.
    SSF103039. SSF103039. 1 hit.
    TIGRFAMsi TIGR01397. fliM_switch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "The draft genome of Thermotoga maritima MSB8."
      US DOE Joint Genome Institute (JGI-PGF)
      Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Land M.L., Hauser L., Boucher N., Noll K., Woyke T.
      Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099Imported.
    3. "Structure of FliM provides insight into assembly of the switch complex in the bacterial flagella motor."
      Park S.Y., Lowder B., Bilwes A.M., Blair D.F., Crane B.R.
      Proc. Natl. Acad. Sci. U.S.A. 103:11886-11891(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 44-226, INTERACTION WITH CHEY, MUTAGENESIS OF GLU-60.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 46-233 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.
    5. "Structure of flagellar motor proteins in complex allows for insights into motor structure and switching."
      Vartanian A.S., Paz A., Fortgang E.A., Abramson J., Dahlquist F.W.
      J. Biol. Chem. 287:35779-35783(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 46-230 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.

    Entry informationi

    Entry nameiFLIM_THEMA
    AccessioniPrimary (citable) accession number: Q9WZE6
    Secondary accession number(s): G4FDE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2013
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3