Q9WZE6 (FLIM_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flagellar motor switch protein FliM

Gene names

Name:	fliM
Ordered Locus Names:	TM_0679
ORF Names:	ThemaDRAFT_0623

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheX chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation By similarity.
Subunit structure	Interacts (via N-terminus) with unphosphorylated CheY. Interacts (via central domain) with FliG (via central domain or via central domain and C-terminus). Ref.3 Ref.4 Ref.5
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity. Bacterial flagellum basal body By similarity.
Sequence similarities	Belongs to the FliM family.

Ontologies

Keywords
Biological process	Chemotaxis Flagellar rotation
Cellular component	Bacterial flagellum Cell inner membrane Cell membrane Membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW ciliary or flagellar motility Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	bacterial-type flagellum basal body Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 328

328

Flagellar motor switch protein FliM

PRO_0000421240

Regions

Region

1 – 45

45

Interaction with unphosphorylated CheY

Experimental info

Mutagenesis

60

1

E → C: No appreciable effect on the binding affinity for CheY. Ref.3

Secondary structure

1

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328

Helix Strand Turn

Details...

Helix

49 – 73

25

Beta strand

79 – 88

10

Helix

89 – 95

7

Beta strand

101 – 107

7

Beta strand

114 – 118

5

Helix

120 – 130

11

Helix

145 – 165

21

Turn

166 – 169

4

Beta strand

174 – 181

8

Helix

183 – 185

3

Beta strand

193 – 204

12

Beta strand

207 – 216

10

Helix

217 – 220

4

Helix

221 – 223

3

Turn

228 – 232

5

Sequences

	Sequence		Length	Mass (Da)
	Q9WZE6 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 3E122C1FFAEBB093	FASTA	328	37,888
	10 20 30 40 50 60 MSDVLSQEEI NQLIEALMKG ELKEEDLLKE EEEKKVKPYD FKRPSKFSKE QLRTFQMIHE 70 80 90 100 110 120 NFGRALSTYL SGRLRTFVDV EISIDQLTYE EFIRSVMIPS FIVIFTGDVF EGSAIFEMRL 130 140 150 160 170 180 DLFYTMLDII MGGPGENPPN RPPTEIETSI MRKEVTNMLT LLAQAWSDFQ YFIPSIENVE 190 200 210 220 230 240 TNPQFVQIVP PNEIVLLVTA SVSWGEFTSF INVCWPFSLL EPLLEKLSDR FWMMGRKPEK 250 260 270 280 290 300 VEERMEELRK ASQKIPVTVQ AVIGETELRL KEILDLEVGD VIRLGTHYKD EIRIDVEGRP 310 320 KFRGIPGVFK GKYAVKVTGE FTNGGEYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"The draft genome of Thermotoga maritima MSB8." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Land M.L., Hauser L., Boucher N., Noll K., Woyke T. Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]	"Structure of FliM provides insight into assembly of the switch complex in the bacterial flagella motor." Park S.Y., Lowder B., Bilwes A.M., Blair D.F., Crane B.R. Proc. Natl. Acad. Sci. U.S.A. 103:11886-11891(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 44-226, INTERACTION WITH CHEY, MUTAGENESIS OF GLU-60.
[4]	"Architecture of the flagellar rotor." Paul K., Gonzalez-Bonet G., Bilwes A.M., Crane B.R., Blair D. EMBO J. 30:2962-2971(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 46-233 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.
[5]	"Structure of flagellar motor proteins in complex allows for insights into motor structure and switching." Vartanian A.S., Paz A., Fortgang E.A., Abramson J., Dahlquist F.W. J. Biol. Chem. 287:35779-35783(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 46-230 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000512 Genomic DNA. Translation: AAD35762.1.
AGIJ01000001 Genomic DNA. Translation: EHA61380.1.

PIR

E72347.

RefSeq

NP_228487.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HP7	X-ray	2.00	A	44-226	[»]
3SOH	X-ray	3.50	A/C	46-233	[»]
4FHR	X-ray	1.93	A	46-230	[»]

ProteinModelPortal

Q9WZE6.

SMR

Q9WZE6. Positions 44-226.

ModBase

Search...

Protein-protein interaction databases

STRING

243274.TM0679.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD35762; AAD35762; TM_0679.
EHA61380; EHA61380; ThemaDRAFT_0623.

GeneID

898347.

KEGG

tma:TM0679.

PATRIC

23936274. VBITheMar51294_0690.

Phylogenomic databases

KO

K02416.

OMA

QERIVKG.

ProtClustDB

CLSK875291.

Family and domain databases

InterPro

IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]

Pfam

PF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]

PIRSF

PIRSF002888. FliM. 1 hit.

PRINTS

PR00955. FLGMOTORFLIM.

SUPFAM

SSF101801. SpoA. 1 hit.

TIGRFAMs

TIGR01397. fliM_switch. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9WZE6.

Entry information

Entry name

FLIM_THEMA

Accession

Primary (citable) accession number: Q9WZE6
Secondary accession number(s): G4FDE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2013
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program