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Q9WZE6

- FLIM_THEMA

UniProt

Q9WZE6 - FLIM_THEMA

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Protein

Flagellar motor switch protein FliM

Gene

fliM

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheX chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity).By similarity

GO - Molecular functioni

  1. motor activity Source: InterPro

GO - Biological processi

  1. bacterial-type flagellum-dependent cell motility Source: InterPro
  2. chemotaxis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-704-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar motor switch protein FliMImported
Gene namesi
Name:fliMBy similarity
Ordered Locus Names:TM_0679
ORF Names:ThemaDRAFT_0623
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cell inner membrane By similarity; Peripheral membrane protein By similarity. Bacterial flagellum basal body By similarity

GO - Cellular componenti

  1. bacterial-type flagellum basal body Source: InterPro
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi60 – 601E → C: No appreciable effect on the binding affinity for CheY. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Flagellar motor switch protein FliMPRO_0000421240Add
BLAST

Interactioni

Subunit structurei

Interacts (via N-terminus) with unphosphorylated CheY. Interacts (via central domain) with FliG (via central domain or via central domain and C-terminus).3 Publications

Protein-protein interaction databases

IntActiQ9WZE6. 1 interaction.
MINTiMINT-8212457.
STRINGi243274.TM0679.

Structurei

Secondary structure

1
328
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1610
Helixi49 – 7325
Beta strandi79 – 8810
Helixi89 – 957
Beta strandi101 – 1077
Beta strandi114 – 1185
Helixi120 – 13011
Helixi145 – 16521
Turni166 – 1694
Beta strandi174 – 1818
Helixi183 – 1853
Beta strandi193 – 20412
Beta strandi207 – 21610
Helixi217 – 2204
Helixi221 – 2233
Turni228 – 2325

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HP7X-ray2.00A44-226[»]
3SOHX-ray3.50A/C46-233[»]
4FHRX-ray1.93A46-230[»]
4IGAX-ray1.73B1-20[»]
ProteinModelPortaliQ9WZE6.
SMRiQ9WZE6. Positions 44-226.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZE6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4545Interaction with unphosphorylated CheY1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the FliM family.Sequence Analysis

Phylogenomic databases

InParanoidiQ9WZE6.
KOiK02416.
OMAiLFNMMRR.
OrthoDBiEOG68M4DH.

Family and domain databases

InterProiIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamiPF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]
PIRSFiPIRSF002888. FliM. 1 hit.
PRINTSiPR00955. FLGMOTORFLIM.
SUPFAMiSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsiTIGR01397. fliM_switch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDVLSQEEI NQLIEALMKG ELKEEDLLKE EEEKKVKPYD FKRPSKFSKE
60 70 80 90 100
QLRTFQMIHE NFGRALSTYL SGRLRTFVDV EISIDQLTYE EFIRSVMIPS
110 120 130 140 150
FIVIFTGDVF EGSAIFEMRL DLFYTMLDII MGGPGENPPN RPPTEIETSI
160 170 180 190 200
MRKEVTNMLT LLAQAWSDFQ YFIPSIENVE TNPQFVQIVP PNEIVLLVTA
210 220 230 240 250
SVSWGEFTSF INVCWPFSLL EPLLEKLSDR FWMMGRKPEK VEERMEELRK
260 270 280 290 300
ASQKIPVTVQ AVIGETELRL KEILDLEVGD VIRLGTHYKD EIRIDVEGRP
310 320
KFRGIPGVFK GKYAVKVTGE FTNGGEYE
Length:328
Mass (Da):37,888
Last modified:November 1, 1999 - v1
Checksum:i3E122C1FFAEBB093
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35762.1.
AGIJ01000001 Genomic DNA. Translation: EHA61380.1.
PIRiE72347.
RefSeqiNP_228487.1. NC_000853.1.
YP_007977029.1. NC_021214.1.
YP_008990352.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35762; AAD35762; TM_0679.
GeneIDi18092110.
898347.
KEGGitma:TM0679.
tmi:THEMA_01270.
tmm:Tmari_0679.
PATRICi23936274. VBITheMar51294_0690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35762.1 .
AGIJ01000001 Genomic DNA. Translation: EHA61380.1 .
PIRi E72347.
RefSeqi NP_228487.1. NC_000853.1.
YP_007977029.1. NC_021214.1.
YP_008990352.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HP7 X-ray 2.00 A 44-226 [» ]
3SOH X-ray 3.50 A/C 46-233 [» ]
4FHR X-ray 1.93 A 46-230 [» ]
4IGA X-ray 1.73 B 1-20 [» ]
ProteinModelPortali Q9WZE6.
SMRi Q9WZE6. Positions 44-226.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WZE6. 1 interaction.
MINTi MINT-8212457.
STRINGi 243274.TM0679.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD35762 ; AAD35762 ; TM_0679 .
GeneIDi 18092110.
898347.
KEGGi tma:TM0679.
tmi:THEMA_01270.
tmm:Tmari_0679.
PATRICi 23936274. VBITheMar51294_0690.

Phylogenomic databases

InParanoidi Q9WZE6.
KOi K02416.
OMAi LFNMMRR.
OrthoDBi EOG68M4DH.

Enzyme and pathway databases

BioCyci TMAR243274:GC6P-704-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9WZE6.

Family and domain databases

InterProi IPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view ]
Pfami PF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view ]
PIRSFi PIRSF002888. FliM. 1 hit.
PRINTSi PR00955. FLGMOTORFLIM.
SUPFAMi SSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsi TIGR01397. fliM_switch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "The draft genome of Thermotoga maritima MSB8."
    US DOE Joint Genome Institute (JGI-PGF)
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Land M.L., Hauser L., Boucher N., Noll K., Woyke T.
    Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099Imported.
  3. "Structure of FliM provides insight into assembly of the switch complex in the bacterial flagella motor."
    Park S.Y., Lowder B., Bilwes A.M., Blair D.F., Crane B.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:11886-11891(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 44-226, INTERACTION WITH CHEY, MUTAGENESIS OF GLU-60.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 46-233 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.
  5. "Structure of flagellar motor proteins in complex allows for insights into motor structure and switching."
    Vartanian A.S., Paz A., Fortgang E.A., Abramson J., Dahlquist F.W.
    J. Biol. Chem. 287:35779-35783(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 46-230 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.

Entry informationi

Entry nameiFLIM_THEMA
AccessioniPrimary (citable) accession number: Q9WZE6
Secondary accession number(s): G4FDE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3