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Q9WZE6 (FLIM_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flagellar motor switch protein FliM
Gene names
Name:fliM
Ordered Locus Names:TM_0679
ORF Names:ThemaDRAFT_0623
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheX chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation By similarity.

Subunit structure

Interacts (via N-terminus) with unphosphorylated CheY. Interacts (via central domain) with FliG (via central domain or via central domain and C-terminus). Ref.3 Ref.4 Ref.5

Subcellular location

Cell inner membrane; Peripheral membrane protein By similarity. Bacterial flagellum basal body By similarity.

Sequence similarities

Belongs to the FliM family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Flagellar motor switch protein FliM
PRO_0000421240

Regions

Region1 – 4545Interaction with unphosphorylated CheY

Experimental info

Mutagenesis601E → C: No appreciable effect on the binding affinity for CheY. Ref.3

Secondary structure

............................. 328
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9WZE6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 3E122C1FFAEBB093

FASTA32837,888
        10         20         30         40         50         60 
MSDVLSQEEI NQLIEALMKG ELKEEDLLKE EEEKKVKPYD FKRPSKFSKE QLRTFQMIHE 

        70         80         90        100        110        120 
NFGRALSTYL SGRLRTFVDV EISIDQLTYE EFIRSVMIPS FIVIFTGDVF EGSAIFEMRL 

       130        140        150        160        170        180 
DLFYTMLDII MGGPGENPPN RPPTEIETSI MRKEVTNMLT LLAQAWSDFQ YFIPSIENVE 

       190        200        210        220        230        240 
TNPQFVQIVP PNEIVLLVTA SVSWGEFTSF INVCWPFSLL EPLLEKLSDR FWMMGRKPEK 

       250        260        270        280        290        300 
VEERMEELRK ASQKIPVTVQ AVIGETELRL KEILDLEVGD VIRLGTHYKD EIRIDVEGRP 

       310        320 
KFRGIPGVFK GKYAVKVTGE FTNGGEYE 

« Hide

References

« Hide 'large scale' references
[1]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"The draft genome of Thermotoga maritima MSB8."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Land M.L., Hauser L., Boucher N., Noll K., Woyke T.
Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]"Structure of FliM provides insight into assembly of the switch complex in the bacterial flagella motor."
Park S.Y., Lowder B., Bilwes A.M., Blair D.F., Crane B.R.
Proc. Natl. Acad. Sci. U.S.A. 103:11886-11891(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 44-226, INTERACTION WITH CHEY, MUTAGENESIS OF GLU-60.
[4]"Architecture of the flagellar rotor."
Paul K., Gonzalez-Bonet G., Bilwes A.M., Crane B.R., Blair D.
EMBO J. 30:2962-2971(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 46-233 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.
[5]"Structure of flagellar motor proteins in complex allows for insights into motor structure and switching."
Vartanian A.S., Paz A., Fortgang E.A., Abramson J., Dahlquist F.W.
J. Biol. Chem. 287:35779-35783(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 46-230 IN COMPLEX WITH FLIG, INTERACTION WITH FLIG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD35762.1.
AGIJ01000001 Genomic DNA. Translation: EHA61380.1.
PIRE72347.
RefSeqNP_228487.1. NC_000853.1.
YP_007977029.1. NC_021214.1.
YP_008990352.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HP7X-ray2.00A44-226[»]
3SOHX-ray3.50A/C46-233[»]
4FHRX-ray1.93A46-230[»]
4IGAX-ray1.73B1-20[»]
ProteinModelPortalQ9WZE6.
SMRQ9WZE6. Positions 44-226.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9WZE6. 1 interaction.
MINTMINT-8212457.
STRING243274.TM0679.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35762; AAD35762; TM_0679.
EHA61380; EHA61380; ThemaDRAFT_0623.
GeneID15494282.
18092110.
898347.
KEGGtma:TM0679.
tmm:Tmari_0679.
PATRIC23936274. VBITheMar51294_0690.

Phylogenomic databases

KOK02416.
OMAQERIVKG.
OrthoDBEOG68M4DH.
ProtClustDBCLSK875291.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-704-MONOMER.

Family and domain databases

InterProIPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamPF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]
PIRSFPIRSF002888. FliM. 1 hit.
PRINTSPR00955. FLGMOTORFLIM.
SUPFAMSSF101801. SSF101801. 1 hit.
TIGRFAMsTIGR01397. fliM_switch. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9WZE6.

Entry information

Entry nameFLIM_THEMA
AccessionPrimary (citable) accession number: Q9WZE6
Secondary accession number(s): G4FDE5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references