Reviewed, UniProtKB/Swiss-Prot Q9WZC2 (SPEE_THEMA)

Names and origin

Protein names

Recommended name:
    Spermidine synthase
    EC=2.5.1.16
Alternative name(s):
    Putrescine aminopropyltransferase
      Short name=PAPT
    SPDSY

Gene names

Name:	speE
Ordered Locus Names:	TM_0654

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor. Ref.2
Catalytic activity	S-adenosylmethioninamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine. HAMAP MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP MF_00198
Subunit structure	Homotetramer. Ref.2
Sequence similarities	Belongs to the spermidine/spermine synthase family.
Biophysicochemical properties	Kinetic parameters: KM=20 µM for putrescine HAMAP MF_00198 pH dependence: Optimum pH is 7.5. Temperature dependence: Optimum temperature is 90 degrees Celsius.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	spermidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 296

296

Spermidine synthase HAMAP MF_00198

PRO_0000156513

Regions

Region

152 – 153

2

S-adenosylmethioninamine binding HAMAP MF_00198

Sites

Binding site

46

1

S-adenosylmethioninamine HAMAP MF_00198

Binding site

101

1

S-adenosylmethioninamine HAMAP MF_00198

Binding site

121

1

S-adenosylmethioninamine HAMAP MF_00198

Binding site

170

1

S-adenosylmethioninamine HAMAP MF_00198

Binding site

173

1

Putrescine HAMAP MF_00198

Secondary structure

1

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296

Helix Strand Turn

Details...

Helix

4 – 7

4

Beta strand

15 – 22

8

Beta strand

26 – 33

8

Beta strand

35 – 42

8

Beta strand

47 – 53

7

Turn

54 – 56

3

Beta strand

57 – 62

6

Beta strand

65 – 69

5

Turn

70 – 72

3

Helix

73 – 87

15

Beta strand

88 – 90

3

Beta strand

93 – 98

6

Helix

103 – 108

6

Beta strand

115 – 122

8

Helix

124 – 133

10

Helix

135 – 138

4

Helix

139 – 142

4

Beta strand

146 – 151

6

Helix

153 – 156

4

Helix

157 – 159

3

Beta strand

164 – 170

7

Helix

185 – 194

10

Beta strand

195 – 204

10

Turn

208 – 211

4

Helix

212 – 225

14

Beta strand

227 – 235

9

Beta strand

242 – 252

11

Turn

255 – 258

4

Helix

261 – 265

5

Helix

276 – 281

6

Helix

287 – 292

6

Sequences

	Sequence		Length	Mass (Da)
	Q9WZC2-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 9298A156B7481945	FASTA	296	34,142
	10 20 30 40 50 60 MRTLKELERE LQPRQHLWYF EYYTGNNVGL FMKMNRVIYS GQSDIQRIDI FENPDLGVVF 70 80 90 100 110 120 ALDGITMTTE KDEFMYHEML AHVPMFLHPN PKKVLIIGGG DGGTLREVLK HDSVEKAILC 130 140 150 160 170 180 EVDGLVIEAA RKYLKQTSCG FDDPRAEIVI ANGAEYVRKF KNEFDVIIID STDPTAGQGG 190 200 210 220 230 240 HLFTEEFYQA CYDALKEDGV FSAETEDPFY DIGWFKLAYR RISKVFPITR VYLGFMTTYP 250 260 270 280 290 SGMWSYTFAS KGIDPIKDFD PEKVRKFNKE LKYYNEEVHV ASFALPNFVK KELGLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor." Korolev S., Ikeguchi Y., Skarina T., Beasley S., Arrowsmith C., Edwards A., Joachimiak A., Pegg A.E., Savchenko A. Nat. Struct. Biol. 9:27-31(2002) [PubMed: 11731804] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF APOENZYME AND COMPLEX WITH SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000512 Genomic DNA. Translation: AAD35738.1.

PIR

C72348.

RefSeq

NP_228463.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1INL	X-ray	1.50	A/B/C/D	1-296	[»]
1JQ3	X-ray	1.80	A/B/C/D	1-296	[»]

ModBase

Search...

Genome annotation databases

GeneID

897762.

GenomeReviews

Gene locus TM_0654 in contig AE000512_GR.

KEGG

tma:TM0654.

NMPDR

fig|243274.1.peg.647.

TIGR

TM_0654.

Phylogenomic databases

HOGENOM

Q9WZC2.

OMA

Q9WZC2. QHESPYY.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_0654-MON.

BRENDA

2.5.1.16. 16699.

Family and domain databases

HAMAP

MF_00198.
[Tree]

InterPro

IPR001045. Sprmine_synthase.
[Graphical view]

PANTHER

PTHR11558. Sprmine_synthase. 1 hit.

Pfam

PF01564. Spermine_synth. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00417. speE. 1 hit.

PROSITE

PS01330. SPERMIDINE_SYNTHASE_1. 1 hit.
PS51006. SPERMIDINE_SYNTHASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SPEE_THEMA

Accession

Primary (citable) accession number: Q9WZC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)