Q9WZB3 (NADE1_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE1
Ordered Locus Names:	TM_0645

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152212
Regions
	Nucleotide binding	24 – 31	8	ATP By similarity
Sites
	Active site	26	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WZB3 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: CAF86CBF207752FA	FASTA	281	32,697
	10 20 30 40 50 60 MKESLISFIK EKIEEYNYRG AVVGVSGGVD SAVVLSLCVQ ALGKDRVFAL ILPERDSSKD 70 80 90 100 110 120 SLKDAVDFCE RLGVEYRKRS ITPILRKIGV YRLFPPRLFL PDSIVKRYVL NRWNTLSKDP 130 140 150 160 170 180 FLDDLRNTGP EEFLKGLAYY RIKHRIRMCL LYFEAEKRGY AVVGTTNRTE YLTGLYVKWG 190 200 210 220 230 240 DEAVDIEPIM HLYKTQVFEL AKEMNVPEKI LKKPPSPDLI PGITDEMAFN MSYLELDRIL 250 260 270 280 MKLEKNEDLS DEDPKKVERV KKILELSEKY RRDIPITFDR I

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD35729.1.
PIR	D72351.
RefSeq	NP_228454.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9WZB3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897750.
GenomeReviews	Gene locus TM_0645 in contig AE000512_GR.
KEGG	tma:TM0645.
NMPDR	fig|243274.1.peg.638.
PATRIC	23936204. VBITheMar51294_0655.
TIGR	TM_0645.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
PhylomeDB	Q9WZB3.
ProtClustDB	CLSK875266.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0645-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 2 hits.
ProtoNet	Search...

Entry information

Entry name

NADE1_THEMA

Accession

Primary (citable) accession number: Q9WZB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program