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Protein

ATP-dependent zinc metalloprotease FtsH

Gene

ftsH

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641ATP; via amide nitrogen and carbonyl oxygen
Binding sitei209 – 2091ATP; via amide nitrogen
Binding sitei343 – 3431ATP
Binding sitei371 – 3711ATP
Metal bindingi423 – 4231Zinc; catalytic
Active sitei424 – 4241UniRule annotation
Metal bindingi427 – 4271Zinc; catalytic
Metal bindingi500 – 5001Zinc; catalytic

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi204 – 2085ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B17. 6331.
3.4.24.B20. 6331.

Protein family/group databases

MEROPSiM41.021.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent zinc metalloprotease FtsHUniRule annotation (EC:3.4.24.-UniRule annotation)
Gene namesi
Name:ftsHUniRule annotation
Ordered Locus Names:TM_0580
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicUniRule annotation
Transmembranei6 – 2621HelicalUniRule annotationAdd
BLAST
Topological domaini27 – 10781PeriplasmicUniRule annotationAdd
BLAST
Transmembranei108 – 12821HelicalUniRule annotationAdd
BLAST
Topological domaini129 – 610482CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi404 – 4041G → L: Complete loss of protease activity and of oligomerization. 1 Publication
Mutagenesisi500 – 5001D → A: Complete loss of protease activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610ATP-dependent zinc metalloprotease FtsHPRO_0000400412Add
BLAST

Interactioni

Subunit structurei

The isolated ADP-bound cytosolic domain forms a 6-fold symmetric protease disk and a 2-fold symmetric AAA ATPase ring. In the absence of nucleotide the AAA ATPase ring also forms symmetric hexamers.2 Publications

Protein-protein interaction databases

DIPiDIP-49026N.
STRINGi243274.TM0580.

Structurei

Secondary structure

1
610
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi38 – 469Combined sources
Beta strandi52 – 576Combined sources
Beta strandi61 – 677Combined sources
Beta strandi72 – 765Combined sources
Helixi78 – 803Combined sources
Helixi84 – 929Combined sources
Beta strandi96 – 994Combined sources
Helixi160 – 1623Combined sources
Helixi167 – 18115Combined sources
Helixi184 – 1874Combined sources
Turni188 – 1903Combined sources
Beta strandi195 – 2006Combined sources
Helixi207 – 21812Combined sources
Beta strandi222 – 2265Combined sources
Helixi227 – 2293Combined sources
Turni230 – 2323Combined sources
Helixi237 – 25115Combined sources
Beta strandi254 – 2607Combined sources
Helixi262 – 2643Combined sources
Helixi279 – 29315Combined sources
Helixi296 – 2983Combined sources
Beta strandi300 – 3078Combined sources
Helixi309 – 3113Combined sources
Helixi314 – 3174Combined sources
Beta strandi324 – 3274Combined sources
Helixi333 – 34412Combined sources
Helixi355 – 3606Combined sources
Helixi367 – 38317Combined sources
Beta strandi387 – 3893Combined sources
Helixi391 – 40111Combined sources
Helixi414 – 43320Combined sources
Beta strandi442 – 4443Combined sources
Helixi453 – 4564Combined sources
Helixi469 – 47911Combined sources
Helixi481 – 49010Combined sources
Helixi495 – 4973Combined sources
Helixi498 – 51316Combined sources
Turni519 – 5213Combined sources
Helixi547 – 57428Combined sources
Helixi576 – 58914Combined sources
Beta strandi590 – 5934Combined sources
Helixi594 – 6007Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CE7X-ray2.44A/B/C/D/E/F147-610[»]
2CEAX-ray2.75A/B/C/D/E/F147-610[»]
3KDSX-ray2.60E/F/G147-610[»]
4M8AX-ray1.50A/B/C34-101[»]
4Q0FX-ray1.95A/B/C34-101[»]
ProteinModelPortaliQ9WZ49.
SMRiQ9WZ49. Positions 150-606.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZ49.

Family & Domainsi

Sequence similaritiesi

In the central section; belongs to the AAA ATPase family.UniRule annotation
In the C-terminal section; belongs to the peptidase M41 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3H. Bacteria.
COG0465. LUCA.
InParanoidiQ9WZ49.
KOiK03798.
OMAiKSHRETH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WZ49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRSNIWNLL FTILIIVTLF WLARFFYVEN SPVSKLSYTS FVQMVEDERS
60 70 80 90 100
VVSEVVIRDD GVLRVYTKDG RVYEVDAPWA VNDSQLIEKL VSKGIKVSGE
110 120 130 140 150
RSGSSSFWIN VLGTLIPTIL FIVVWLFIMR SLSGRNNQAF TFTKSRATMY
160 170 180 190 200
KPSGNKRVTF KDVGGAEEAI EELKEVVEFL KDPSKFNRIG ARMPKGILLV
210 220 230 240 250
GPPGTGKTLL ARAVAGEANV PFFHISGSDF VELFVGVGAA RVRDLFAQAK
260 270 280 290 300
AHAPCIVFID EIDAVGRHRG AGLGGGHDER EQTLNQLLVE MDGFDSKEGI
310 320 330 340 350
IVMAATNRPD ILDPALLRPG RFDKKIVVDP PDMLGRKKIL EIHTRNKPLA
360 370 380 390 400
EDVNLEIIAK RTPGFVGADL ENLVNEAALL AAREGRDKIT MKDFEEAIDR
410 420 430 440 450
VIAGPARKSK LISPKEKRII AYHEAGHAVV STVVPNGEPV HRISIIPRGY
460 470 480 490 500
KALGYTLHLP EEDKYLVSRN ELLDKLTALL GGRAAEEVVF GDVTSGAAND
510 520 530 540 550
IERATEIARN MVCQLGMSEE LGPLAWGKEE QEVFLGKEIT RLRNYSEEVA
560 570 580 590 600
SKIDEEVKKI VTNCYERAKE IIRKYRKQLD NIVEILLEKE TIEGDELRRI
610
LSEEFEKVVE
Length:610
Mass (Da):68,099
Last modified:November 1, 1999 - v1
Checksum:i83F76468C8495DDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35665.1.
PIRiE72358.
RefSeqiNP_228390.1. NC_000853.1.
WP_004081278.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35665; AAD35665; TM_0580.
GeneIDi897649.
KEGGitma:TM0580.
tmi:THEMA_01765.
tmw:THMA_0595.
PATRICi23936071. VBITheMar51294_0589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35665.1.
PIRiE72358.
RefSeqiNP_228390.1. NC_000853.1.
WP_004081278.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CE7X-ray2.44A/B/C/D/E/F147-610[»]
2CEAX-ray2.75A/B/C/D/E/F147-610[»]
3KDSX-ray2.60E/F/G147-610[»]
4M8AX-ray1.50A/B/C34-101[»]
4Q0FX-ray1.95A/B/C34-101[»]
ProteinModelPortaliQ9WZ49.
SMRiQ9WZ49. Positions 150-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-49026N.
STRINGi243274.TM0580.

Protein family/group databases

MEROPSiM41.021.

Protocols and materials databases

DNASUi897649.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35665; AAD35665; TM_0580.
GeneIDi897649.
KEGGitma:TM0580.
tmi:THEMA_01765.
tmw:THMA_0595.
PATRICi23936071. VBITheMar51294_0589.

Phylogenomic databases

eggNOGiENOG4105C3H. Bacteria.
COG0465. LUCA.
InParanoidiQ9WZ49.
KOiK03798.
OMAiKSHRETH.

Enzyme and pathway databases

BRENDAi3.4.24.B17. 6331.
3.4.24.B20. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZ49.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFTSH_THEMA
AccessioniPrimary (citable) accession number: Q9WZ49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.