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Protein

S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Gene

queA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA.

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.4.99.17. 6331.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC:2.4.99.17)
Alternative name(s):
Queuosine biosynthesis protein QueA
Gene namesi
Name:queA
Ordered Locus Names:TM_0574
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335S-adenosylmethionine:tRNA ribosyltransferase-isomerasePRO_0000165458Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM0574.

Structurei

Secondary structure

1
335
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 143Combined sources
Helixi23 – 253Combined sources
Beta strandi26 – 327Combined sources
Turni33 – 364Combined sources
Beta strandi37 – 426Combined sources
Helixi43 – 497Combined sources
Beta strandi55 – 639Combined sources
Beta strandi67 – 715Combined sources
Beta strandi78 – 8710Combined sources
Beta strandi90 – 978Combined sources
Beta strandi106 – 1105Combined sources
Beta strandi113 – 1208Combined sources
Beta strandi126 – 1327Combined sources
Helixi135 – 1417Combined sources
Helixi175 – 1784Combined sources
Helixi181 – 19010Combined sources
Beta strandi193 – 2019Combined sources
Beta strandi223 – 2264Combined sources
Helixi228 – 24013Combined sources
Beta strandi244 – 2485Combined sources
Helixi249 – 25810Combined sources
Beta strandi265 – 2695Combined sources
Beta strandi285 – 2906Combined sources
Helixi297 – 30610Combined sources
Helixi308 – 32013Combined sources
Beta strandi331 – 3355Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VKYX-ray2.00A/B1-335[»]
ProteinModelPortaliQ9WZ44.
SMRiQ9WZ44. Positions 4-335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZ44.

Family & Domainsi

Sequence similaritiesi

Belongs to the QueA family.Curated

Phylogenomic databases

eggNOGiENOG4105E2N. Bacteria.
COG0809. LUCA.
InParanoidiQ9WZ44.
KOiK07568.
OMAiPYIKRDY.
OrthoDBiEOG6X6RBB.

Family and domain databases

HAMAPiMF_00113. QueA.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZ44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSEFDYEL PPELIAQEPV EPRDASRLMV LHRKTQRIEH RIFREIIEYL
60 70 80 90 100
EPGDLLVLNV SKVIPARLYA RKKTGASIEI LLIERLEEGI WKCLVRPGQK
110 120 130 140 150
VKKGTELVID EDLSAVCLGR GEDGTRILKF QPQDDRLIFE KGRTPLPPYI
160 170 180 190 200
KNEVPLERYQ TVYAKEEGSV AAPTAGLHFT PELIEKLKKK GVQFAEVVLH
210 220 230 240 250
VGIGTFRPVK VEEVEKHKMH EEFYQVPKET VRKLRETRER GNRIVAVGTT
260 270 280 290 300
TVRTLETIAR LPEQEEYVGK TDLFIYPPFE FKLVDALVTN FHLPRSTLLM
310 320 330
LVAAFAGKDF VMEAYREAVK RRYRFFSFGD AMLIL
Length:335
Mass (Da):38,662
Last modified:November 1, 1999 - v1
Checksum:i8D634537041B13E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35659.1.
PIRiA72360.
RefSeqiNP_228384.1. NC_000853.1.
WP_004081288.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35659; AAD35659; TM_0574.
GeneIDi897639.
KEGGitma:TM0574.
PATRICi23936057. VBITheMar51294_0583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35659.1.
PIRiA72360.
RefSeqiNP_228384.1. NC_000853.1.
WP_004081288.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VKYX-ray2.00A/B1-335[»]
ProteinModelPortaliQ9WZ44.
SMRiQ9WZ44. Positions 4-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35659; AAD35659; TM_0574.
GeneIDi897639.
KEGGitma:TM0574.
PATRICi23936057. VBITheMar51294_0583.

Phylogenomic databases

eggNOGiENOG4105E2N. Bacteria.
COG0809. LUCA.
InParanoidiQ9WZ44.
KOiK07568.
OMAiPYIKRDY.
OrthoDBiEOG6X6RBB.

Enzyme and pathway databases

UniPathwayiUPA00392.
BRENDAi2.4.99.17. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZ44.

Family and domain databases

HAMAPiMF_00113. QueA.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiQUEA_THEMA
AccessioniPrimary (citable) accession number: Q9WZ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.