Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Gene

queA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA.

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.4.99.17. 6331.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC:2.4.99.17)
Alternative name(s):
Queuosine biosynthesis protein QueA
Gene namesi
Name:queA
Ordered Locus Names:TM_0574
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001654581 – 335S-adenosylmethionine:tRNA ribosyltransferase-isomeraseAdd BLAST335

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM0574.

Structurei

Secondary structure

1335
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 14Combined sources3
Helixi23 – 25Combined sources3
Beta strandi26 – 32Combined sources7
Turni33 – 36Combined sources4
Beta strandi37 – 42Combined sources6
Helixi43 – 49Combined sources7
Beta strandi55 – 63Combined sources9
Beta strandi67 – 71Combined sources5
Beta strandi78 – 87Combined sources10
Beta strandi90 – 97Combined sources8
Beta strandi106 – 110Combined sources5
Beta strandi113 – 120Combined sources8
Beta strandi126 – 132Combined sources7
Helixi135 – 141Combined sources7
Helixi175 – 178Combined sources4
Helixi181 – 190Combined sources10
Beta strandi193 – 201Combined sources9
Beta strandi223 – 226Combined sources4
Helixi228 – 240Combined sources13
Beta strandi244 – 248Combined sources5
Helixi249 – 258Combined sources10
Beta strandi265 – 269Combined sources5
Beta strandi285 – 290Combined sources6
Helixi297 – 306Combined sources10
Helixi308 – 320Combined sources13
Beta strandi331 – 335Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VKYX-ray2.00A/B1-335[»]
ProteinModelPortaliQ9WZ44.
SMRiQ9WZ44.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WZ44.

Family & Domainsi

Sequence similaritiesi

Belongs to the QueA family.Curated

Phylogenomic databases

eggNOGiENOG4105E2N. Bacteria.
COG0809. LUCA.
InParanoidiQ9WZ44.
KOiK07568.
OMAiNTFLVFN.

Family and domain databases

HAMAPiMF_00113. QueA. 1 hit.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WZ44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSEFDYEL PPELIAQEPV EPRDASRLMV LHRKTQRIEH RIFREIIEYL
60 70 80 90 100
EPGDLLVLNV SKVIPARLYA RKKTGASIEI LLIERLEEGI WKCLVRPGQK
110 120 130 140 150
VKKGTELVID EDLSAVCLGR GEDGTRILKF QPQDDRLIFE KGRTPLPPYI
160 170 180 190 200
KNEVPLERYQ TVYAKEEGSV AAPTAGLHFT PELIEKLKKK GVQFAEVVLH
210 220 230 240 250
VGIGTFRPVK VEEVEKHKMH EEFYQVPKET VRKLRETRER GNRIVAVGTT
260 270 280 290 300
TVRTLETIAR LPEQEEYVGK TDLFIYPPFE FKLVDALVTN FHLPRSTLLM
310 320 330
LVAAFAGKDF VMEAYREAVK RRYRFFSFGD AMLIL
Length:335
Mass (Da):38,662
Last modified:November 1, 1999 - v1
Checksum:i8D634537041B13E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35659.1.
PIRiA72360.
RefSeqiNP_228384.1. NC_000853.1.
WP_004081288.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35659; AAD35659; TM_0574.
GeneIDi897639.
KEGGitma:TM0574.
PATRICi23936057. VBITheMar51294_0583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35659.1.
PIRiA72360.
RefSeqiNP_228384.1. NC_000853.1.
WP_004081288.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VKYX-ray2.00A/B1-335[»]
ProteinModelPortaliQ9WZ44.
SMRiQ9WZ44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35659; AAD35659; TM_0574.
GeneIDi897639.
KEGGitma:TM0574.
PATRICi23936057. VBITheMar51294_0583.

Phylogenomic databases

eggNOGiENOG4105E2N. Bacteria.
COG0809. LUCA.
InParanoidiQ9WZ44.
KOiK07568.
OMAiNTFLVFN.

Enzyme and pathway databases

UniPathwayiUPA00392.
BRENDAi2.4.99.17. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WZ44.

Family and domain databases

HAMAPiMF_00113. QueA. 1 hit.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEA_THEMA
AccessioniPrimary (citable) accession number: Q9WZ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.