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Q9WZ26 (LEU3_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
Ordered Locus Names:TM_0556
OrganismThermotoga maritima
Taxonomic identifier2336 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3543543-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000083771

Regions

Nucleotide binding74 – 8714NAD By similarity
Nucleotide binding275 – 28713NAD By similarity

Sites

Metal binding2191Magnesium or manganese By similarity
Metal binding2431Magnesium or manganese By similarity
Metal binding2471Magnesium or manganese By similarity
Binding site951Substrate By similarity
Binding site1051Substrate By similarity
Binding site1341Substrate By similarity
Binding site2191Substrate By similarity
Site1411Important for catalysis By similarity
Site1871Important for catalysis By similarity

Secondary structure

1 ................................................................... 354
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9WZ26 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 2C93ACBF888A0D38

FASTA35439,190
        10         20         30         40         50         60 
MKIAVLPGDG IGPEVVREAL KVLEVVEKKT GKTFEKVFGH IGGDAIDRFG EPLPEETKKI 

        70         80         90        100        110        120 
CLEADAIFLG SVGGPKWDDL PPEKRPEIGG LLALRKMLNL YANIRPIKVY RSLVHVSPLK 

       130        140        150        160        170        180 
EKVIGSGVDL VTVRELSYGV YYGQPRGLDE EKGFDTMIYD RKTVERIART AFEIAKNRRK 

       190        200        210        220        230        240 
KVTSVDKANV LYSSMLWRKV VNEVAREYPD VELTHIYVDN AAMQLILKPS QFDVILTTNM 

       250        260        270        280        290        300 
FGDILSDESA ALPGSLGLLP SASFGDKNLY EPAGGSAPDI AGKNIANPIA QILSLAMMLE 

       310        320        330        340        350 
HSFGMVEEAR KIERAVELVI EEGYRTRDIA EDPEKAVSTS QMGDLICKKL EEIW

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000512 Genomic DNA. Translation: AAD35641.1.
PIRB72363.
RefSeqNP_228366.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VLCX-ray1.90A1-354[»]
ProteinModelPortalQ9WZ26.
SMRQ9WZ26. Positions 1-354.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID897614.
GenomeReviewsGene locus TM_0556 in contig AE000512_GR.
KEGGtma:TM0556.
NMPDRfig|243274.1.peg.550.
PATRIC23936019. VBITheMar51294_0564.
TIGRTM_0556.

Phylogenomic databases

HOGENOMHBG518924.
OMAAAMMLRF.
PhylomeDBQ9WZ26.
ProtClustDBPRK00772.

Enzyme and pathway databases

BioCycTMAR243274:TM_0556-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_THEMA
AccessionPrimary (citable) accession number: Q9WZ26
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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