Malate oxidoreductase - Thermotoga maritima

Preferred order of sections

Names and origin

Protein names

Submitted name:
Malate oxidoreductase EMBL AAD35627.1

Gene names

Ordered Locus Names:

TM_0542

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the malic enzymes family. RuleBase RU003427

Ontologies

Keywords
Ligand	Metal-binding RuleBase RU003427 PDB 2HAE NAD PDB 2HAE Nucleotide-binding PDB 2HAE Zinc PDB 2HAE
Molecular function	Oxidoreductase RuleBase RU003427
Technical term	3D-structure PDB 2HAE PDB 1VL6 Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

186 – 191

6

NAD PDB 2HAE

Nucleotide binding

211 – 212

2

NAD PDB 2HAE

Nucleotide binding

259 – 260

2

NAD PDB 2HAE

Nucleotide binding

281 – 283

3

NAD PDB 2HAE

Sites

Metal binding

87

1

Zinc PDB 2HAE

Metal binding

129

1

Zinc PDB 2HAE

Metal binding

130

1

Zinc PDB 2HAE

Metal binding

155

1

Zinc PDB 2HAE

Binding site

159

1

NAD PDB 2HAE

Binding site

314

1

NAD PDB 2HAE

Sequences

Sequence

Length

Mass (Da)

Tools

Q9WZ12 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 101A5506E3938600
Show »

FASTA

376

41,043

        10         20         30         40         50         60 
MDALEIHRFL KGKIRTALPV EKVDRETLSL LYTPGVADVA RACAEDPEKT YVYTSRWNTV 

        70         80         90        100        110        120 
AVVSDGSAVL GLGNIGPYGA LPVMEGKAFL FKAFADIDAF PICLSESEEE KIISIVKSLE 

       130        140        150        160        170        180 
PSFGGINLED IGAPKCFRIL QRLSEEMNIP VFHDDQQGTA VVVSAAFLNA LKLTEKKIEE 

       190        200        210        220        230        240 
VKVVVNGIGA AGYNIVKFLL DLGVKNVVAV DRKGILNEND PETCLNEYHL EIARITNPER 

       250        260        270        280        290        300 
LSGDLETALE GADFFIGVSR GNILKPEWIK KMSRKPVIFA LANPVPEIDP ELAREAGAFI 

       310        320        330        340        350        360 
VATGRSDHPN QVNNLLAFPG IMKGAVEKRS KITKNMLLSA VEAIARSCEP EPERIIPEAF 

       370 
DMKVHLNVYT AVKGSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Crystal structure of a putative malic enzyme (malate oxidoreductase)." Seetharaman J., Swaminathan S. Submitted (JUN-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (3.13 ANGSTROMS) OF 2-376 IN COMPLEX WITH NAD AND ZINC.
[3]	"Crystal structure of NAD-dependent malic enzyme (TM0542) from Thermotoga maritima at 2.61 A resolution." Joint Center for Structural Genomics (JCSG) Submitted (JUL-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.61 ANGSTROMS) OF 2-376.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000512 Genomic DNA. Translation: AAD35627.1.

PIR

C72364.

RefSeq

NP_228352.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VL6	X-ray	2.61	A/B/C/D	2-376	[»]
2HAE	X-ray	3.13	A/B/C/D	2-376	[»]

ProteinModelPortal

Q9WZ12.

SMR

Q9WZ12. Positions 1-376.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

897596.

GenomeReviews

Gene locus TM_0542 in contig AE000512_GR.

KEGG

tma:TM0542.

NMPDR

fig|243274.1.peg.536.

PATRIC

23935991. VBITheMar51294_0550.

TIGR

TM_0542.

Phylogenomic databases

HOGENOM

HBG285702.

OMA

CEGETWM.

PhylomeDB

Q9WZ12.

ProtClustDB

CLSK875209.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_0542-MONOMER.

Family and domain databases

InterPro

IPR012301. Malic_N.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K00027.

Pfam

PF00390. malic. 2 hits.
PF03949. Malic_M. 1 hit.
[Graphical view]

PIRSF

PIRSF000106. ME. 1 hit.

SMART

SM00919. Malic_M. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9WZ12_THEMA

Accession

Primary (citable) accession number: Q9WZ12

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1999
Last sequence update:	November 1, 1999
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)