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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.
L-glutamine + H2O = L-glutamate + NH3.

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Nucleophile1
Binding sitei105L-glutamine1
Active sitei170Charge relay system1
Active sitei172Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxT (EC:4.3.3.6)
Alternative name(s):
Pdx2
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2)
Gene namesi
Name:pdxT
Synonyms:yaaE1 Publication
Ordered Locus Names:TM_0472
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356691 – 188Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST188

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.1 Publication

Protein-protein interaction databases

STRINGi243274.TM0472.

Structurei

Secondary structure

1188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Beta strandi8 – 10Combined sources3
Helixi12 – 21Combined sources10
Beta strandi25 – 29Combined sources5
Helixi32 – 37Combined sources6
Beta strandi39 – 43Combined sources5
Helixi48 – 57Combined sources10
Helixi61 – 69Combined sources9
Beta strandi74 – 77Combined sources4
Helixi79 – 84Combined sources6
Beta strandi85 – 88Combined sources4
Beta strandi99 – 104Combined sources6
Turni105 – 108Combined sources4
Helixi111 – 113Combined sources3
Beta strandi115 – 119Combined sources5
Helixi122 – 124Combined sources3
Beta strandi129 – 135Combined sources7
Beta strandi138 – 142Combined sources5
Beta strandi147 – 152Combined sources6
Beta strandi155 – 161Combined sources7
Beta strandi164 – 169Combined sources6
Helixi171 – 173Combined sources3
Helixi178 – 184Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ISSX-ray2.90D/E/F1-188[»]
ProteinModelPortaliQ9WYU3.
SMRiQ9WYU3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYU3.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 48L-glutamine binding3
Regioni134 – 135L-glutamine binding2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX. Bacteria.
COG0311. LUCA.
InParanoidiQ9WYU3.
KOiK08681.
OMAiVFIRAPI.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
PANTHERiPTHR31559. PTHR31559. 1 hit.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WYU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGVLGVQG DVREHVEALH KLGVETLIVK LPEQLDMVDG LILPGGESTT
60 70 80 90 100
MIRILKEMDM DEKLVERINN GLPVFATCAG VILLAKRIKN YSQEKLGVLD
110 120 130 140 150
ITVERNAYGR QVESFETFVE IPAVGKDPFR AIFIRAPRIV ETGKNVEILA
160 170 180
TYDYDPVLVK EGNILACTFH PELTDDLRLH RYFLEMVK
Length:188
Mass (Da):21,178
Last modified:November 1, 1999 - v1
Checksum:iAAE2FA50E81F8348
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35557.1.
PIRiH72371.
RefSeqiNP_228282.1. NC_000853.1.
WP_004081494.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35557; AAD35557; TM_0472.
GeneIDi897504.
KEGGitma:TM0472.

Similar proteinsi

Entry informationi

Entry nameiPDXT_THEMA
AccessioniPrimary (citable) accession number: Q9WYU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: November 1, 1999
Last modified: November 22, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families