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Protein

Glycerol dehydrogenase

Gene

gldA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions (By similarity).By similarity

Catalytic activityi

Glycerol + NAD+ = glycerone + NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathway: glycerol fermentation

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycerol dehydrogenase (gldA)
  2. no protein annotated in this organism
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371NADBy similarity
Binding sitei119 – 1191SubstrateCurated
Binding sitei123 – 1231NADBy similarity
Binding sitei125 – 1251NAD; via carbonyl oxygenBy similarity
Binding sitei129 – 1291NADBy similarity
Metal bindingi169 – 1691Zinc; catalytic1 Publication
Binding sitei169 – 1691SubstrateCurated
Metal bindingi252 – 2521Zinc; catalytic1 Publication
Binding sitei252 – 2521SubstrateCurated
Metal bindingi269 – 2691Zinc; catalytic1 Publication
Binding sitei269 – 2691SubstrateCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 965NADBy similarity
Nucleotide bindingi114 – 1174NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

UniPathwayiUPA00617; UER00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol dehydrogenase (EC:1.1.1.6)
Short name:
GDH
Short name:
GLDH
Gene namesi
Name:gldA
Ordered Locus Names:TM_0423
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Glycerol dehydrogenasePRO_0000350665Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0423.

Structurei

Secondary structure

1
364
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 146Combined sources
Helixi17 – 204Combined sources
Helixi21 – 266Combined sources
Beta strandi30 – 367Combined sources
Helixi38 – 436Combined sources
Helixi49 – 524Combined sources
Beta strandi54 – 629Combined sources
Helixi69 – 768Combined sources
Beta strandi85 – 917Combined sources
Helixi92 – 10413Combined sources
Beta strandi109 – 1157Combined sources
Beta strandi123 – 1297Combined sources
Beta strandi135 – 1406Combined sources
Beta strandi146 – 1516Combined sources
Helixi152 – 1576Combined sources
Helixi160 – 18324Combined sources
Beta strandi190 – 1923Combined sources
Helixi195 – 22026Combined sources
Helixi226 – 24621Combined sources
Helixi250 – 2589Combined sources
Helixi262 – 2643Combined sources
Helixi269 – 28315Combined sources
Helixi288 – 30114Combined sources
Helixi307 – 3104Combined sources
Helixi317 – 32711Combined sources
Helixi333 – 3364Combined sources
Beta strandi337 – 3393Combined sources
Helixi343 – 36018Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KQ3X-ray1.50A1-364[»]
ProteinModelPortaliQ9WYQ4.
SMRiQ9WYQ4. Positions 1-363.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYQ4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0371.
InParanoidiQ9WYQ4.
KOiK00005.
OMAiYFEARAN.
OrthoDBiEOG67432X.

Family and domain databases

InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016205. Glycerol_DH.
[Graphical view]
PfamiPF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000112. Glycerol_dehydrogenase. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITTTIFPGR YVQGAGAINI LEEELSRFGE RAFVVIDDFV DKNVLGENFF
60 70 80 90 100
SSFTKVRVNK QIFGGECSDE EIERLSGLVE EETDVVVGIG GGKTLDTAKA
110 120 130 140 150
VAYKLKKPVV IVPTIASTDA PCSALSVIYT PNGEFKRYLF LPRNPDVVLV
160 170 180 190 200
DTEIVAKAPA RFLVAGMGDA LATWFEAESC KQKYAPNMTG RLGSMTAYAL
210 220 230 240 250
ARLCYETLLE YGVLAKRSVE EKSVTPALEK IVEANTLLSG LGFESGGLAA
260 270 280 290 300
AHAIHNGLTV LENTHKYLHG EKVAIGVLAS LFLTDKPRKM IEEVYSFCEE
310 320 330 340 350
VGLPTTLAEI GLDGVSDEDL MKVAEKACDK NETIHNEPQP VTSKDVFFAL
360
KAADRYGRMR KNLT
Length:364
Mass (Da):39,692
Last modified:November 1, 1999 - v1
Checksum:iB44E20CACC6F1582
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35508.1.
PIRiG72378.
RefSeqiNP_228233.1. NC_000853.1.
WP_004083283.1. NC_023151.1.
YP_007976770.1. NC_021214.1.
YP_008990607.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35508; AAD35508; TM_0423.
GeneIDi897429.
KEGGitma:TM0423.
tmi:THEMA_02610.
tmm:Tmari_0420.
PATRICi23935729. VBITheMar51294_0428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35508.1.
PIRiG72378.
RefSeqiNP_228233.1. NC_000853.1.
WP_004083283.1. NC_023151.1.
YP_007976770.1. NC_021214.1.
YP_008990607.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KQ3X-ray1.50A1-364[»]
ProteinModelPortaliQ9WYQ4.
SMRiQ9WYQ4. Positions 1-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35508; AAD35508; TM_0423.
GeneIDi897429.
KEGGitma:TM0423.
tmi:THEMA_02610.
tmm:Tmari_0420.
PATRICi23935729. VBITheMar51294_0428.

Phylogenomic databases

eggNOGiCOG0371.
InParanoidiQ9WYQ4.
KOiK00005.
OMAiYFEARAN.
OrthoDBiEOG67432X.

Enzyme and pathway databases

UniPathwayiUPA00617; UER00668.

Miscellaneous databases

EvolutionaryTraceiQ9WYQ4.

Family and domain databases

InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016205. Glycerol_DH.
[Graphical view]
PfamiPF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000112. Glycerol_dehydrogenase. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH ZINC AND SUBSTRATE ANALOG.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiGLDA_THEMA
AccessioniPrimary (citable) accession number: Q9WYQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: November 1, 1999
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.