Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol dehydrogenase

Gene

gldA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions (By similarity).By similarity

Catalytic activityi

Glycerol + NAD+ = glycerone + NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: glycerol fermentation

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycerol dehydrogenase (gldA)
  2. no protein annotated in this organism
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADBy similarity1
Binding sitei119SubstrateCurated1
Binding sitei123NADBy similarity1
Binding sitei125NAD; via carbonyl oxygenBy similarity1
Binding sitei129NADBy similarity1
Metal bindingi169Zinc; catalytic1 Publication1
Binding sitei169SubstrateCurated1
Metal bindingi252Zinc; catalytic1 Publication1
Binding sitei252SubstrateCurated1
Metal bindingi269Zinc; catalytic1 Publication1
Binding sitei269SubstrateCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 96NADBy similarity5
Nucleotide bindingi114 – 117NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

UniPathwayiUPA00617; UER00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol dehydrogenase (EC:1.1.1.6)
Short name:
GDH
Short name:
GLDH
Gene namesi
Name:gldA
Ordered Locus Names:TM_0423
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003506651 – 364Glycerol dehydrogenaseAdd BLAST364

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0423.

Structurei

Secondary structure

1364
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 14Combined sources6
Helixi17 – 20Combined sources4
Helixi21 – 26Combined sources6
Beta strandi30 – 36Combined sources7
Helixi38 – 43Combined sources6
Helixi49 – 52Combined sources4
Beta strandi54 – 62Combined sources9
Helixi69 – 76Combined sources8
Beta strandi85 – 91Combined sources7
Helixi92 – 104Combined sources13
Beta strandi109 – 115Combined sources7
Beta strandi123 – 129Combined sources7
Beta strandi135 – 140Combined sources6
Beta strandi146 – 151Combined sources6
Helixi152 – 157Combined sources6
Helixi160 – 183Combined sources24
Beta strandi190 – 192Combined sources3
Helixi195 – 220Combined sources26
Helixi226 – 246Combined sources21
Helixi250 – 258Combined sources9
Helixi262 – 264Combined sources3
Helixi269 – 283Combined sources15
Helixi288 – 301Combined sources14
Helixi307 – 310Combined sources4
Helixi317 – 327Combined sources11
Helixi333 – 336Combined sources4
Beta strandi337 – 339Combined sources3
Helixi343 – 360Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KQ3X-ray1.50A1-364[»]
ProteinModelPortaliQ9WYQ4.
SMRiQ9WYQ4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYQ4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DCT. Bacteria.
COG0371. LUCA.
InParanoidiQ9WYQ4.
KOiK00005.
OMAiCTKAVEN.

Family and domain databases

InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
[Graphical view]
PfamiPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITTTIFPGR YVQGAGAINI LEEELSRFGE RAFVVIDDFV DKNVLGENFF
60 70 80 90 100
SSFTKVRVNK QIFGGECSDE EIERLSGLVE EETDVVVGIG GGKTLDTAKA
110 120 130 140 150
VAYKLKKPVV IVPTIASTDA PCSALSVIYT PNGEFKRYLF LPRNPDVVLV
160 170 180 190 200
DTEIVAKAPA RFLVAGMGDA LATWFEAESC KQKYAPNMTG RLGSMTAYAL
210 220 230 240 250
ARLCYETLLE YGVLAKRSVE EKSVTPALEK IVEANTLLSG LGFESGGLAA
260 270 280 290 300
AHAIHNGLTV LENTHKYLHG EKVAIGVLAS LFLTDKPRKM IEEVYSFCEE
310 320 330 340 350
VGLPTTLAEI GLDGVSDEDL MKVAEKACDK NETIHNEPQP VTSKDVFFAL
360
KAADRYGRMR KNLT
Length:364
Mass (Da):39,692
Last modified:November 1, 1999 - v1
Checksum:iB44E20CACC6F1582
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35508.1.
PIRiG72378.
RefSeqiNP_228233.1. NC_000853.1.
WP_004083283.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35508; AAD35508; TM_0423.
GeneIDi897429.
KEGGitma:TM0423.
PATRICi23935729. VBITheMar51294_0428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35508.1.
PIRiG72378.
RefSeqiNP_228233.1. NC_000853.1.
WP_004083283.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KQ3X-ray1.50A1-364[»]
ProteinModelPortaliQ9WYQ4.
SMRiQ9WYQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35508; AAD35508; TM_0423.
GeneIDi897429.
KEGGitma:TM0423.
PATRICi23935729. VBITheMar51294_0428.

Phylogenomic databases

eggNOGiENOG4105DCT. Bacteria.
COG0371. LUCA.
InParanoidiQ9WYQ4.
KOiK00005.
OMAiCTKAVEN.

Enzyme and pathway databases

UniPathwayiUPA00617; UER00668.

Miscellaneous databases

EvolutionaryTraceiQ9WYQ4.

Family and domain databases

InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
[Graphical view]
PfamiPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLDA_THEMA
AccessioniPrimary (citable) accession number: Q9WYQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.