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Q9WYN2

- KITH_THEMA

UniProt

Q9WYN2 - KITH_THEMA

Protein

Thymidine kinase

Gene

tdk

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Kineticsi

    1. KM=40 µM for ATP1 Publication
    2. KM=0.5 µM for thymidine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531ATP
    Active sitei84 – 841Proton acceptorSequence Analysis
    Binding sitei115 – 1151Substrate; via amide nitrogen
    Metal bindingi140 – 1401Zinc
    Metal bindingi143 – 1431Zinc
    Binding sitei169 – 1691Substrate
    Metal bindingi173 – 1731Zinc
    Metal bindingi176 – 1761Zinc

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178ATP
    Nucleotide bindingi83 – 864ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:tdk
    Ordered Locus Names:TM_0401
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi53 – 531H → A: Reduced affinity for ATP. 1 Publication
    Mutagenesisi55 – 551G → W: Reduced affinity for ATP. 1 Publication
    Mutagenesisi129 – 1291L → W: Reduced affinity for thymidine. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 184184Thymidine kinasePRO_0000175040Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    DIPiDIP-29527N.
    STRINGi243274.TM0401.

    Structurei

    Secondary structure

    1
    184
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 117
    Helixi16 – 2914
    Beta strandi33 – 397
    Beta strandi61 – 655
    Helixi66 – 727
    Beta strandi77 – 826
    Helixi85 – 873
    Helixi92 – 10110
    Beta strandi105 – 1139
    Helixi121 – 1299
    Beta strandi131 – 1355
    Turni141 – 1433
    Beta strandi146 – 1483
    Beta strandi150 – 1534
    Turni166 – 1683
    Beta strandi169 – 1724
    Helixi174 – 1807

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ORWX-ray1.50A/B1-184[»]
    2QPOX-ray1.95A/B/C/D1-184[»]
    2QQ0X-ray1.50A/B1-184[»]
    2QQEX-ray1.90A/B1-184[»]
    ProteinModelPortaliQ9WYN2.
    SMRiQ9WYN2. Positions 2-182.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WYN2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni161 – 1644Substrate binding

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1435.
    KOiK00857.
    OMAiQAICLSC.
    OrthoDBiEOG69D3J2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WYN2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGKLTVITG PMYSGKTTEL LSFVEIYKLG KKKVAVFKPK IDSRYHSTMI    50
    VSHSGNGVEA HVIERPEEMR KYIEEDTRGV FIDEVQFFNP SLFEVVKDLL 100
    DRGIDVFCAG LDLTHKQNPF ETTALLLSLA DTVIKKKAVC HRCGEYNATL 150
    TLKVAGGEEE IDVGGQEKYI AVCRDCYNTL KKRV 184
    Length:184
    Mass (Da):20,654
    Last modified:November 1, 1999 - v1
    Checksum:i906B2C1BE3A7EDDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35486.1.
    PIRiB72383.
    RefSeqiNP_228211.1. NC_000853.1.
    YP_007976748.1. NC_021214.1.
    YP_008990629.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35486; AAD35486; TM_0401.
    GeneIDi897394.
    KEGGitma:TM0401.
    tmi:THEMA_02720.
    tmm:Tmari_0398.
    PATRICi23935685. VBITheMar51294_0406.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35486.1 .
    PIRi B72383.
    RefSeqi NP_228211.1. NC_000853.1.
    YP_007976748.1. NC_021214.1.
    YP_008990629.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ORW X-ray 1.50 A/B 1-184 [» ]
    2QPO X-ray 1.95 A/B/C/D 1-184 [» ]
    2QQ0 X-ray 1.50 A/B 1-184 [» ]
    2QQE X-ray 1.90 A/B 1-184 [» ]
    ProteinModelPortali Q9WYN2.
    SMRi Q9WYN2. Positions 2-182.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29527N.
    STRINGi 243274.TM0401.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35486 ; AAD35486 ; TM_0401 .
    GeneIDi 897394.
    KEGGi tma:TM0401.
    tmi:THEMA_02720.
    tmm:Tmari_0398.
    PATRICi 23935685. VBITheMar51294_0406.

    Phylogenomic databases

    eggNOGi COG1435.
    KOi K00857.
    OMAi QAICLSC.
    OrthoDBi EOG69D3J2.

    Miscellaneous databases

    EvolutionaryTracei Q9WYN2.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure."
      Segura-Pena D., Lutz S., Monnerjahn C., Konrad M., Lavie A.
      J. Mol. Biol. 369:129-141(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH ZINC IONS; THYMIDINE AND ATP ANALOGS, SUBUNIT.
    3. "Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes."
      Segura-Pena D., Lichter J., Trani M., Konrad M., Lavie A., Lutz S.
      Structure 15:1555-1566(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND THYMIDINE, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF HIS-53; GLY-55 AND LEU-129.

    Entry informationi

    Entry nameiKITH_THEMA
    AccessioniPrimary (citable) accession number: Q9WYN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3