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Protein

Thymidine kinase

Gene

tdk

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Kineticsi

  1. KM=40 µM for ATP1 Publication
  2. KM=0.5 µM for thymidine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei53ATP1
    Active sitei84Proton acceptorSequence analysis1
    Binding sitei115Substrate; via amide nitrogen1
    Metal bindingi140Zinc1
    Metal bindingi143Zinc1
    Binding sitei169Substrate1
    Metal bindingi173Zinc1
    Metal bindingi176Zinc1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 17ATP8
    Nucleotide bindingi83 – 86ATPBy similarity4

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:tdk
    Ordered Locus Names:TM_0401
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    Proteomesi
    • UP000008183 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi53H → A: Reduced affinity for ATP. 1 Publication1
    Mutagenesisi55G → W: Reduced affinity for ATP. 1 Publication1
    Mutagenesisi129L → W: Reduced affinity for thymidine. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001750401 – 184Thymidine kinaseAdd BLAST184

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    DIPiDIP-29527N.
    STRINGi243274.TM0401.

    Structurei

    Secondary structure

    1184
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 11Combined sources7
    Helixi16 – 29Combined sources14
    Beta strandi33 – 39Combined sources7
    Beta strandi61 – 65Combined sources5
    Helixi66 – 72Combined sources7
    Beta strandi77 – 82Combined sources6
    Helixi85 – 87Combined sources3
    Helixi92 – 101Combined sources10
    Beta strandi105 – 113Combined sources9
    Helixi121 – 129Combined sources9
    Beta strandi131 – 135Combined sources5
    Turni141 – 143Combined sources3
    Beta strandi146 – 148Combined sources3
    Beta strandi150 – 153Combined sources4
    Turni166 – 168Combined sources3
    Beta strandi169 – 172Combined sources4
    Helixi174 – 180Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2ORWX-ray1.50A/B1-184[»]
    2QPOX-ray1.95A/B/C/D1-184[»]
    2QQ0X-ray1.50A/B1-184[»]
    2QQEX-ray1.90A/B1-184[»]
    ProteinModelPortaliQ9WYN2.
    SMRiQ9WYN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WYN2.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni161 – 164Substrate binding4

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    eggNOGiENOG4107104. Bacteria.
    COG1435. LUCA.
    InParanoidiQ9WYN2.
    KOiK00857.
    OMAiVITGPMY.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00124. Thymidine_kinase. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WYN2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSGKLTVITG PMYSGKTTEL LSFVEIYKLG KKKVAVFKPK IDSRYHSTMI
    60 70 80 90 100
    VSHSGNGVEA HVIERPEEMR KYIEEDTRGV FIDEVQFFNP SLFEVVKDLL
    110 120 130 140 150
    DRGIDVFCAG LDLTHKQNPF ETTALLLSLA DTVIKKKAVC HRCGEYNATL
    160 170 180
    TLKVAGGEEE IDVGGQEKYI AVCRDCYNTL KKRV
    Length:184
    Mass (Da):20,654
    Last modified:November 1, 1999 - v1
    Checksum:i906B2C1BE3A7EDDA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35486.1.
    PIRiB72383.
    RefSeqiNP_228211.1. NC_000853.1.
    WP_004083238.1. NZ_CP011107.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35486; AAD35486; TM_0401.
    GeneIDi897394.
    KEGGitma:TM0401.
    PATRICi23935685. VBITheMar51294_0406.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35486.1.
    PIRiB72383.
    RefSeqiNP_228211.1. NC_000853.1.
    WP_004083238.1. NZ_CP011107.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2ORWX-ray1.50A/B1-184[»]
    2QPOX-ray1.95A/B/C/D1-184[»]
    2QQ0X-ray1.50A/B1-184[»]
    2QQEX-ray1.90A/B1-184[»]
    ProteinModelPortaliQ9WYN2.
    SMRiQ9WYN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-29527N.
    STRINGi243274.TM0401.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD35486; AAD35486; TM_0401.
    GeneIDi897394.
    KEGGitma:TM0401.
    PATRICi23935685. VBITheMar51294_0406.

    Phylogenomic databases

    eggNOGiENOG4107104. Bacteria.
    COG1435. LUCA.
    InParanoidiQ9WYN2.
    KOiK00857.
    OMAiVITGPMY.

    Miscellaneous databases

    EvolutionaryTraceiQ9WYN2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00124. Thymidine_kinase. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiKITH_THEMA
    AccessioniPrimary (citable) accession number: Q9WYN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: November 2, 2016
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.