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Protein

Endonuclease III

Gene

nth

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi180Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi187Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi190Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi196Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:TM_0366
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022241 – 213Endonuclease IIIAdd BLAST213

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0366

Structurei

3D structure databases

ProteinModelPortaliQ9WYK0
SMRiQ9WYK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 120HhHUniRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSM Bacteria
COG0177 LUCA
InParanoidiQ9WYK0
KOiK10773
OMAiWQQFTHL

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR01083 nth, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WYK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEELAREIV KRFPRNHKET DPFRVLISTV LSQRTRDENT EKASKKLFEV
60 70 80 90 100
YRTPQELAKA KPEDLYDLIK ESGMYRQKAE RIVEISRILV EKYGGRVPDS
110 120 130 140 150
LEELLKLPGV GRKTANIVLW VGFKKPALAV DTHVHRISNR LGWVKTRTPE
160 170 180 190 200
ETEEALKKLL PEDLWGPING SMVEFGRRIC KPQNPLCEEC FLKNHCEFYR
210
RRGKGEVRNR TER
Length:213
Mass (Da):24,928
Last modified:November 1, 1999 - v1
Checksum:iD9716CD6FEFA85D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD35453.1
PIRiF72387
RefSeqiNP_228177.1, NC_000853.1
WP_004083173.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD35453; AAD35453; TM_0366
GeneIDi897325
KEGGitma:TM0366

Similar proteinsi

Entry informationi

Entry nameiEND3_THEMA
AccessioniPrimary (citable) accession number: Q9WYK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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