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Q9WYK0

- END3_THEMA

UniProt

Q9WYK0 - END3_THEMA

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Protein
Endonuclease III
Gene
nth, TM_0366
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi180 – 1801Iron-sulfur (4Fe-4S) By similarity
Metal bindingi187 – 1871Iron-sulfur (4Fe-4S) By similarity
Metal bindingi190 – 1901Iron-sulfur (4Fe-4S) By similarity
Metal bindingi196 – 1961Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III (EC:4.2.99.18)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene namesi
Name:nth
Ordered Locus Names:TM_0366
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Endonuclease IIIUniRule annotation
PRO_0000102224Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0366.

Structurei

3D structure databases

ProteinModelPortaliQ9WYK0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 12020HhH
Add
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.
Contains 1 HhH domain.

Phylogenomic databases

eggNOGiCOG0177.
KOiK10773.
OMAiVDAHHWL.
OrthoDBiEOG6H4KC5.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYK0-1 [UniParc]FASTAAdd to Basket

« Hide

MIEELAREIV KRFPRNHKET DPFRVLISTV LSQRTRDENT EKASKKLFEV    50
YRTPQELAKA KPEDLYDLIK ESGMYRQKAE RIVEISRILV EKYGGRVPDS 100
LEELLKLPGV GRKTANIVLW VGFKKPALAV DTHVHRISNR LGWVKTRTPE 150
ETEEALKKLL PEDLWGPING SMVEFGRRIC KPQNPLCEEC FLKNHCEFYR 200
RRGKGEVRNR TER 213
Length:213
Mass (Da):24,928
Last modified:November 1, 1999 - v1
Checksum:iD9716CD6FEFA85D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35453.1.
PIRiF72387.
RefSeqiNP_228177.1. NC_000853.1.
YP_007976714.1. NC_021214.1.
YP_008990661.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35453; AAD35453; TM_0366.
GeneIDi897325.
KEGGitma:TM0366.
tmi:THEMA_02885.
tmm:Tmari_0364.
PATRICi23935613. VBITheMar51294_0371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35453.1 .
PIRi F72387.
RefSeqi NP_228177.1. NC_000853.1.
YP_007976714.1. NC_021214.1.
YP_008990661.1. NC_023151.1.

3D structure databases

ProteinModelPortali Q9WYK0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM0366.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD35453 ; AAD35453 ; TM_0366 .
GeneIDi 897325.
KEGGi tma:TM0366.
tmi:THEMA_02885.
tmm:Tmari_0364.
PATRICi 23935613. VBITheMar51294_0371.

Phylogenomic databases

eggNOGi COG0177.
KOi K10773.
OMAi VDAHHWL.
OrthoDBi EOG6H4KC5.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_00942. Nth.
InterProi IPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view ]
Pfami PF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001435. Nth. 1 hit.
SMARTi SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
TIGRFAMsi TIGR01083. nth. 1 hit.
PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiEND3_THEMA
AccessioniPrimary (citable) accession number: Q9WYK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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