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Q9WYJ7

- END4_THEMA

UniProt

Q9WYJ7 - END4_THEMA

Protein

Probable endonuclease 4

Gene

nfo

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

    Cofactori

    Binds 3 zinc ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691Zinc 1UniRule annotation
    Metal bindingi109 – 1091Zinc 1UniRule annotation
    Metal bindingi144 – 1441Zinc 1UniRule annotation
    Metal bindingi144 – 1441Zinc 2UniRule annotation
    Metal bindingi178 – 1781Zinc 2UniRule annotation
    Metal bindingi181 – 1811Zinc 3UniRule annotation
    Metal bindingi215 – 2151Zinc 2UniRule annotation
    Metal bindingi228 – 2281Zinc 3UniRule annotation
    Metal bindingi230 – 2301Zinc 3UniRule annotation
    Metal bindingi260 – 2601Zinc 2UniRule annotation

    GO - Molecular functioni

    1. deoxyribonuclease IV (phage-T4-induced) activity Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-376-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
    Alternative name(s):
    Endodeoxyribonuclease IVUniRule annotation
    Endonuclease IVUniRule annotation
    Gene namesi
    Name:nfoUniRule annotation
    Ordered Locus Names:TM_0362
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 287287Probable endonuclease 4PRO_0000190880Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243274.TM0362.

    Structurei

    Secondary structure

    1
    287
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Helixi14 – 163
    Helixi17 – 237
    Beta strandi27 – 315
    Beta strandi37 – 393
    Helixi46 – 5813
    Helixi63 – 653
    Beta strandi66 – 694
    Helixi81 – 10121
    Beta strandi105 – 1084
    Helixi118 – 13316
    Beta strandi140 – 1445
    Helixi157 – 16610
    Helixi170 – 1723
    Beta strandi173 – 1786
    Helixi179 – 1846
    Helixi192 – 20514
    Helixi208 – 2103
    Beta strandi211 – 2166
    Beta strandi218 – 2214
    Beta strandi235 – 2384
    Helixi239 – 2479
    Helixi250 – 2534
    Beta strandi257 – 2593
    Helixi265 – 28117

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2X7VX-ray2.30A1-287[»]
    2X7WX-ray2.36A1-287[»]
    4HNOX-ray0.92A2-285[»]
    ProteinModelPortaliQ9WYJ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WYJ7.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AP endonuclease 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0648.
    KOiK01151.
    OMAiIAWLKSE.
    OrthoDBiEOG6Z0QCM.

    Family and domain databases

    Gene3Di3.20.20.150. 1 hit.
    HAMAPiMF_00152. Nfo.
    InterProiIPR001719. AP_endonuc_2.
    IPR018246. AP_endonuc_F2_Zn_BS.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view]
    PANTHERiPTHR21445. PTHR21445. 1 hit.
    PfamiPF01261. AP_endonuc_2. 1 hit.
    [Graphical view]
    SMARTiSM00518. AP2Ec. 1 hit.
    [Graphical view]
    SUPFAMiSSF51658. SSF51658. 1 hit.
    TIGRFAMsiTIGR00587. nfo. 1 hit.
    PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
    PS00730. AP_NUCLEASE_F2_2. 1 hit.
    PS00731. AP_NUCLEASE_F2_3. 1 hit.
    PS51432. AP_NUCLEASE_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WYJ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKIGAHMPI SKGFDRVPQD TVNIGGNSFQ IFPHNARSWS AKLPSDEAAT    50
    KFKREMKKHG IDWENAFCHS GYLINLASPK DDIWQKSVEL LKKEVEICRK 100
    LGIRYLNIHP GSHLGTGEEE GIDRIVRGLN EVLNNTEGVV ILLENVSQKG 150
    GNIGYKLEQL KKIRDLVDQR DRVAITYDTC HGFDSGYDIT KKEGVEALLN 200
    EIESLFGLER LKMIHLNDSK YPLGAAKDRH ERIGSGFIGE EGFAVFFSFK 250
    EIQEVPWILE TPGGNEEHAE DIKKVFEIIE KFGIEVD 287
    Length:287
    Mass (Da):32,435
    Last modified:November 1, 1999 - v1
    Checksum:iA2BEE00CE7AE4A5A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35449.1.
    PIRiB72387.
    RefSeqiNP_228173.1. NC_000853.1.
    WP_010865102.1. NC_023151.1.
    YP_007976710.1. NC_021214.1.
    YP_008990665.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35449; AAD35449; TM_0362.
    GeneIDi897321.
    KEGGitma:TM0362.
    tmi:THEMA_02905.
    tmm:Tmari_0360.
    PATRICi23935605. VBITheMar51294_0367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35449.1 .
    PIRi B72387.
    RefSeqi NP_228173.1. NC_000853.1.
    WP_010865102.1. NC_023151.1.
    YP_007976710.1. NC_021214.1.
    YP_008990665.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2X7V X-ray 2.30 A 1-287 [» ]
    2X7W X-ray 2.36 A 1-287 [» ]
    4HNO X-ray 0.92 A 2-285 [» ]
    ProteinModelPortali Q9WYJ7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0362.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35449 ; AAD35449 ; TM_0362 .
    GeneIDi 897321.
    KEGGi tma:TM0362.
    tmi:THEMA_02905.
    tmm:Tmari_0360.
    PATRICi 23935605. VBITheMar51294_0367.

    Phylogenomic databases

    eggNOGi COG0648.
    KOi K01151.
    OMAi IAWLKSE.
    OrthoDBi EOG6Z0QCM.

    Enzyme and pathway databases

    BioCyci TMAR243274:GC6P-376-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9WYJ7.

    Family and domain databases

    Gene3Di 3.20.20.150. 1 hit.
    HAMAPi MF_00152. Nfo.
    InterProi IPR001719. AP_endonuc_2.
    IPR018246. AP_endonuc_F2_Zn_BS.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view ]
    PANTHERi PTHR21445. PTHR21445. 1 hit.
    Pfami PF01261. AP_endonuc_2. 1 hit.
    [Graphical view ]
    SMARTi SM00518. AP2Ec. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51658. SSF51658. 1 hit.
    TIGRFAMsi TIGR00587. nfo. 1 hit.
    PROSITEi PS00729. AP_NUCLEASE_F2_1. 1 hit.
    PS00730. AP_NUCLEASE_F2_2. 1 hit.
    PS00731. AP_NUCLEASE_F2_3. 1 hit.
    PS51432. AP_NUCLEASE_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiEND4_THEMA
    AccessioniPrimary (citable) accession number: Q9WYJ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3