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Q9WYJ7 (END4_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable endonuclease 4
EC=3.1.21.2
Alternative name(s):
Endodeoxyribonuclease IV
Endonuclease IV
Gene names
Name:nfo
Ordered Locus Names:TM_0362
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga ›

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin By similarity. HAMAP-Rule MF_00152

Catalytic activity

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. HAMAP-Rule MF_00152

Cofactor

Binds 3 zinc ions By similarity.

Sequence similarities

Belongs to the AP endonuclease 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Probable endonuclease 4 HAMAP-Rule MF_00152
PRO_0000190880

Sites

Metal binding691Zinc 1 By similarity
Metal binding1091Zinc 1 By similarity
Metal binding1441Zinc 1 By similarity
Metal binding1441Zinc 2 By similarity
Metal binding1781Zinc 2 By similarity
Metal binding1811Zinc 3 By similarity
Metal binding2151Zinc 2 By similarity
Metal binding2281Zinc 3 By similarity
Metal binding2301Zinc 3 By similarity
Metal binding2601Zinc 2 By similarity

Secondary structure

1 ............................................. 287
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9WYJ7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A2BEE00CE7AE4A5A

FASTA28732,435
        10         20         30         40         50         60 
MIKIGAHMPI SKGFDRVPQD TVNIGGNSFQ IFPHNARSWS AKLPSDEAAT KFKREMKKHG 

        70         80         90        100        110        120 
IDWENAFCHS GYLINLASPK DDIWQKSVEL LKKEVEICRK LGIRYLNIHP GSHLGTGEEE 

       130        140        150        160        170        180 
GIDRIVRGLN EVLNNTEGVV ILLENVSQKG GNIGYKLEQL KKIRDLVDQR DRVAITYDTC 

       190        200        210        220        230        240 
HGFDSGYDIT KKEGVEALLN EIESLFGLER LKMIHLNDSK YPLGAAKDRH ERIGSGFIGE 

       250        260        270        280 
EGFAVFFSFK EIQEVPWILE TPGGNEEHAE DIKKVFEIIE KFGIEVD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000512 Genomic DNA. Translation: AAD35449.1.
PIRB72387.
RefSeqNP_228173.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2X7VX-ray2.30A1-287[»]
2X7WX-ray2.36A1-287[»]
4HNOX-ray0.92A1-285[»]
ProteinModelPortalQ9WYJ7.
ModBaseSearch...

Protein-protein interaction databases

STRING243274.TM0362.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35449; AAD35449; TM_0362.
GeneID897321.
KEGGtma:TM0362.
PATRIC23935605. VBITheMar51294_0367.

Phylogenomic databases

eggNOGCOG0648.
KOK01151.
OMAINLGHPD.
ProtClustDBCLSK875108.

Family and domain databases

Gene3D3.20.20.150. 1 hit.
HAMAPMF_00152. Nfo.
InterProIPR018246. AP_endonuc_F2_Zn_BS.
IPR001719. Endodeoxyribonuclease_IV.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERPTHR21445. PTHR21445. 1 hit.
PfamPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMSSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMsTIGR00587. nfo. 1 hit.
PROSITEPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9WYJ7.

Entry information

Entry nameEND4_THEMA
AccessionPrimary (citable) accession number: Q9WYJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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