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Protein

Probable endonuclease 4

Gene

nfo

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1UniRule annotation
Metal bindingi109 – 1091Zinc 1UniRule annotation
Metal bindingi144 – 1441Zinc 1UniRule annotation
Metal bindingi144 – 1441Zinc 2UniRule annotation
Metal bindingi178 – 1781Zinc 2UniRule annotation
Metal bindingi181 – 1811Zinc 3UniRule annotation
Metal bindingi215 – 2151Zinc 2UniRule annotation
Metal bindingi228 – 2281Zinc 3UniRule annotation
Metal bindingi230 – 2301Zinc 3UniRule annotation
Metal bindingi260 – 2601Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-376-MONOMER.
BRENDAi3.1.21.2. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:TM_0362
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Probable endonuclease 4PRO_0000190880Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0362.

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Helixi14 – 163Combined sources
Helixi17 – 237Combined sources
Beta strandi27 – 315Combined sources
Beta strandi37 – 393Combined sources
Helixi46 – 5813Combined sources
Helixi63 – 653Combined sources
Beta strandi66 – 694Combined sources
Helixi81 – 10121Combined sources
Beta strandi105 – 1084Combined sources
Helixi118 – 13316Combined sources
Beta strandi140 – 1445Combined sources
Helixi157 – 16610Combined sources
Helixi170 – 1723Combined sources
Beta strandi173 – 1786Combined sources
Helixi179 – 1846Combined sources
Helixi192 – 20514Combined sources
Helixi208 – 2103Combined sources
Beta strandi211 – 2166Combined sources
Beta strandi218 – 2214Combined sources
Beta strandi235 – 2384Combined sources
Helixi239 – 2479Combined sources
Helixi250 – 2534Combined sources
Beta strandi257 – 2593Combined sources
Helixi265 – 28117Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X7VX-ray2.30A1-287[»]
2X7WX-ray2.36A1-287[»]
4HNOX-ray0.92A2-285[»]
ProteinModelPortaliQ9WYJ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYJ7.

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0648.
InParanoidiQ9WYJ7.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIGAHMPI SKGFDRVPQD TVNIGGNSFQ IFPHNARSWS AKLPSDEAAT
60 70 80 90 100
KFKREMKKHG IDWENAFCHS GYLINLASPK DDIWQKSVEL LKKEVEICRK
110 120 130 140 150
LGIRYLNIHP GSHLGTGEEE GIDRIVRGLN EVLNNTEGVV ILLENVSQKG
160 170 180 190 200
GNIGYKLEQL KKIRDLVDQR DRVAITYDTC HGFDSGYDIT KKEGVEALLN
210 220 230 240 250
EIESLFGLER LKMIHLNDSK YPLGAAKDRH ERIGSGFIGE EGFAVFFSFK
260 270 280
EIQEVPWILE TPGGNEEHAE DIKKVFEIIE KFGIEVD
Length:287
Mass (Da):32,435
Last modified:November 1, 1999 - v1
Checksum:iA2BEE00CE7AE4A5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35449.1.
PIRiB72387.
RefSeqiNP_228173.1. NC_000853.1.
WP_010865102.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35449; AAD35449; TM_0362.
GeneIDi897321.
KEGGitma:TM0362.
tmi:THEMA_02905.
tmm:Tmari_0360.
PATRICi23935605. VBITheMar51294_0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35449.1.
PIRiB72387.
RefSeqiNP_228173.1. NC_000853.1.
WP_010865102.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X7VX-ray2.30A1-287[»]
2X7WX-ray2.36A1-287[»]
4HNOX-ray0.92A2-285[»]
ProteinModelPortaliQ9WYJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35449; AAD35449; TM_0362.
GeneIDi897321.
KEGGitma:TM0362.
tmi:THEMA_02905.
tmm:Tmari_0360.
PATRICi23935605. VBITheMar51294_0367.

Phylogenomic databases

eggNOGiCOG0648.
InParanoidiQ9WYJ7.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-376-MONOMER.
BRENDAi3.1.21.2. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WYJ7.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiEND4_THEMA
AccessioniPrimary (citable) accession number: Q9WYJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: July 22, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.