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Protein

Probable endonuclease 4

Gene

nfo

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1UniRule annotation1
Metal bindingi109Zinc 1UniRule annotation1
Metal bindingi144Zinc 1UniRule annotation1
Metal bindingi144Zinc 2UniRule annotation1
Metal bindingi178Zinc 2UniRule annotation1
Metal bindingi181Zinc 3UniRule annotation1
Metal bindingi215Zinc 2UniRule annotation1
Metal bindingi228Zinc 3UniRule annotation1
Metal bindingi230Zinc 3UniRule annotation1
Metal bindingi260Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.21.2. 6331.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:TM_0362
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908801 – 287Probable endonuclease 4Add BLAST287

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0362.

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Helixi14 – 16Combined sources3
Helixi17 – 23Combined sources7
Beta strandi27 – 31Combined sources5
Beta strandi37 – 39Combined sources3
Helixi46 – 58Combined sources13
Helixi63 – 65Combined sources3
Beta strandi66 – 69Combined sources4
Helixi81 – 101Combined sources21
Beta strandi105 – 108Combined sources4
Helixi118 – 133Combined sources16
Beta strandi140 – 144Combined sources5
Helixi157 – 166Combined sources10
Helixi170 – 172Combined sources3
Beta strandi173 – 178Combined sources6
Helixi179 – 184Combined sources6
Helixi192 – 205Combined sources14
Helixi208 – 210Combined sources3
Beta strandi211 – 216Combined sources6
Beta strandi218 – 221Combined sources4
Beta strandi235 – 238Combined sources4
Helixi239 – 247Combined sources9
Helixi250 – 253Combined sources4
Beta strandi257 – 259Combined sources3
Helixi265 – 281Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X7VX-ray2.30A1-287[»]
2X7WX-ray2.36A1-287[»]
4HNOX-ray0.92A2-285[»]
ProteinModelPortaliQ9WYJ7.
SMRiQ9WYJ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYJ7.

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
InParanoidiQ9WYJ7.
KOiK01151.
OMAiMKYVGAH.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIGAHMPI SKGFDRVPQD TVNIGGNSFQ IFPHNARSWS AKLPSDEAAT
60 70 80 90 100
KFKREMKKHG IDWENAFCHS GYLINLASPK DDIWQKSVEL LKKEVEICRK
110 120 130 140 150
LGIRYLNIHP GSHLGTGEEE GIDRIVRGLN EVLNNTEGVV ILLENVSQKG
160 170 180 190 200
GNIGYKLEQL KKIRDLVDQR DRVAITYDTC HGFDSGYDIT KKEGVEALLN
210 220 230 240 250
EIESLFGLER LKMIHLNDSK YPLGAAKDRH ERIGSGFIGE EGFAVFFSFK
260 270 280
EIQEVPWILE TPGGNEEHAE DIKKVFEIIE KFGIEVD
Length:287
Mass (Da):32,435
Last modified:November 1, 1999 - v1
Checksum:iA2BEE00CE7AE4A5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35449.1.
PIRiB72387.
RefSeqiNP_228173.1. NC_000853.1.
WP_010865102.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35449; AAD35449; TM_0362.
GeneIDi897321.
KEGGitma:TM0362.
PATRICi23935605. VBITheMar51294_0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35449.1.
PIRiB72387.
RefSeqiNP_228173.1. NC_000853.1.
WP_010865102.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X7VX-ray2.30A1-287[»]
2X7WX-ray2.36A1-287[»]
4HNOX-ray0.92A2-285[»]
ProteinModelPortaliQ9WYJ7.
SMRiQ9WYJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35449; AAD35449; TM_0362.
GeneIDi897321.
KEGGitma:TM0362.
PATRICi23935605. VBITheMar51294_0367.

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
InParanoidiQ9WYJ7.
KOiK01151.
OMAiMKYVGAH.

Enzyme and pathway databases

BRENDAi3.1.21.2. 6331.

Miscellaneous databases

EvolutionaryTraceiQ9WYJ7.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEND4_THEMA
AccessioniPrimary (citable) accession number: Q9WYJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.