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Q9WYJ7

- END4_THEMA

UniProt

Q9WYJ7 - END4_THEMA

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Protein
Probable endonuclease 4
Gene
nfo, TM_0362
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Binds 3 zinc ions By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1 By similarity
Metal bindingi109 – 1091Zinc 1 By similarity
Metal bindingi144 – 1441Zinc 1 By similarity
Metal bindingi144 – 1441Zinc 2 By similarity
Metal bindingi178 – 1781Zinc 2 By similarity
Metal bindingi181 – 1811Zinc 3 By similarity
Metal bindingi215 – 2151Zinc 2 By similarity
Metal bindingi228 – 2281Zinc 3 By similarity
Metal bindingi230 – 2301Zinc 3 By similarity
Metal bindingi260 – 2601Zinc 2 By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. deoxyribonuclease IV (phage-T4-induced) activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-376-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4 (EC:3.1.21.2)
Alternative name(s):
Endodeoxyribonuclease IV
Endonuclease IV
Gene namesi
Name:nfo
Ordered Locus Names:TM_0362
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Probable endonuclease 4UniRule annotation
PRO_0000190880Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0362.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Helixi14 – 163
Helixi17 – 237
Beta strandi27 – 315
Beta strandi37 – 393
Helixi46 – 5813
Helixi63 – 653
Beta strandi66 – 694
Helixi81 – 10121
Beta strandi105 – 1084
Helixi118 – 13316
Beta strandi140 – 1445
Helixi157 – 16610
Helixi170 – 1723
Beta strandi173 – 1786
Helixi179 – 1846
Helixi192 – 20514
Helixi208 – 2103
Beta strandi211 – 2166
Beta strandi218 – 2214
Beta strandi235 – 2384
Helixi239 – 2479
Helixi250 – 2534
Beta strandi257 – 2593
Helixi265 – 28117

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X7VX-ray2.30A1-287[»]
2X7WX-ray2.36A1-287[»]
4HNOX-ray0.92A2-285[»]
ProteinModelPortaliQ9WYJ7.

Miscellaneous databases

EvolutionaryTraceiQ9WYJ7.

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.

Phylogenomic databases

eggNOGiCOG0648.
KOiK01151.
OMAiIAWLKSE.
OrthoDBiEOG6Z0QCM.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYJ7-1 [UniParc]FASTAAdd to Basket

« Hide

MIKIGAHMPI SKGFDRVPQD TVNIGGNSFQ IFPHNARSWS AKLPSDEAAT    50
KFKREMKKHG IDWENAFCHS GYLINLASPK DDIWQKSVEL LKKEVEICRK 100
LGIRYLNIHP GSHLGTGEEE GIDRIVRGLN EVLNNTEGVV ILLENVSQKG 150
GNIGYKLEQL KKIRDLVDQR DRVAITYDTC HGFDSGYDIT KKEGVEALLN 200
EIESLFGLER LKMIHLNDSK YPLGAAKDRH ERIGSGFIGE EGFAVFFSFK 250
EIQEVPWILE TPGGNEEHAE DIKKVFEIIE KFGIEVD 287
Length:287
Mass (Da):32,435
Last modified:November 1, 1999 - v1
Checksum:iA2BEE00CE7AE4A5A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35449.1.
PIRiB72387.
RefSeqiNP_228173.1. NC_000853.1.
WP_010865102.1. NC_023151.1.
YP_007976710.1. NC_021214.1.
YP_008990665.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35449; AAD35449; TM_0362.
GeneIDi897321.
KEGGitma:TM0362.
tmi:THEMA_02905.
tmm:Tmari_0360.
PATRICi23935605. VBITheMar51294_0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35449.1 .
PIRi B72387.
RefSeqi NP_228173.1. NC_000853.1.
WP_010865102.1. NC_023151.1.
YP_007976710.1. NC_021214.1.
YP_008990665.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2X7V X-ray 2.30 A 1-287 [» ]
2X7W X-ray 2.36 A 1-287 [» ]
4HNO X-ray 0.92 A 2-285 [» ]
ProteinModelPortali Q9WYJ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM0362.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD35449 ; AAD35449 ; TM_0362 .
GeneIDi 897321.
KEGGi tma:TM0362.
tmi:THEMA_02905.
tmm:Tmari_0360.
PATRICi 23935605. VBITheMar51294_0367.

Phylogenomic databases

eggNOGi COG0648.
KOi K01151.
OMAi IAWLKSE.
OrthoDBi EOG6Z0QCM.

Enzyme and pathway databases

BioCyci TMAR243274:GC6P-376-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9WYJ7.

Family and domain databases

Gene3Di 3.20.20.150. 1 hit.
HAMAPi MF_00152. Nfo.
InterProi IPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view ]
PANTHERi PTHR21445. PTHR21445. 1 hit.
Pfami PF01261. AP_endonuc_2. 1 hit.
[Graphical view ]
SMARTi SM00518. AP2Ec. 1 hit.
[Graphical view ]
SUPFAMi SSF51658. SSF51658. 1 hit.
TIGRFAMsi TIGR00587. nfo. 1 hit.
PROSITEi PS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiEND4_THEMA
AccessioniPrimary (citable) accession number: Q9WYJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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