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Reviewed, UniProtKB/Swiss-Prot Q9WYI3 (AROKB_THEMA)

Last modified November 3, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bifunctional shikimate kinase/3-dehydroquinate synthase
Including the following 2 domains:
    1- Recommended name:
            Shikimate kinase
                Short name=SK
              EC=2.7.1.71
    2- Recommended name:
            3-dehydroquinate synthase
              EC=4.2.3.4
Gene names
Name: aroKB
Ordered Locus Names: TM_0348
OrganismThermotoga maritima [Complete proteome] [HAMAP]
Taxonomic identifier2336 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

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Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.

Catalytic activity

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. HAMAP MF_00109

ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109

Cofactor

Binds 1 magnesium ion per subunit By similarity.

NAD By similarity.

Divalent metal cations By similarity.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. HAMAP MF_00109

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.

Subcellular location

Cytoplasm Probable.

Sequence similarities

In the N-terminal section; belongs to the shikimate kinase family.

In the C-terminal section; belongs to the dehydroquinate synthase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Bifunctional shikimate kinase/3-dehydroquinate synthase HAMAP MF_00109
PRO_0000192432

Regions

Nucleotide binding10 – 156ATP By similarity
Region1 – 161161Shikimate kinase HAMAP MF_00109
Region162 – 4923313-dehydroquinate synthase HAMAP MF_00109

Sites

Metal binding141Magnesium By similarity
Binding site321Substrate By similarity
Binding site561Substrate By similarity
Binding site781Substrate; via amide nitrogen By similarity
Binding site1141ATP By similarity
Binding site1311Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9WYI3-1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: F04C7058237C1C1A

FASTA49256,385
        10         20         30         40         50         60 
MRIFLVGMMG SGKSTIGKRI SEVLDLQFID MDEEIERREG RSVRRIFEED GEEYFRLKEK 

        70         80         90        100        110        120 
ELLKELVERD NVVVATGGGV VVDPENRELL KKEKTLFLYA PPEVLMERVT TENRPLLSEG 

       130        140        150        160        170        180 
KERIREIWEK RKQFYAEFRR IDTSRLNEWE TTALVVLEAL DEKEISTIEK PHLVKIILGG 

       190        200        210        220        230        240 
FKRVRNEELV FTTERVEKIY GRYLPENRLL FPDGEEVKTL EHVSRAYYEL IRMDFPRGKT 

       250        260        270        280        290        300 
IAGVGGGALT DFTGFVASTF KRGVGLSFYP TTLLAQVDAS VGGKNAIDFA GVKNVVGTFR 

       310        320        330        340        350        360 
MPDYVIIDPT VTLSMDEGRF EEGVVEAFKM TILSGRGVEL FDEPEKIEKR NLRVLSEMVK 

       370        380        390        400        410        420 
ISVEEKARIV MEDPYDMGLR HALNLGHTLG HVYEMLEGVP HGIAVAWGIE KETMYLYRKG 

       430        440        450        460        470        480 
IVPKETMRWI VEKVKQIVPI PVPSVDVEKA RNLILNDKKI LKGSRVRLPY VKEIGKIEFL 

       490 
EVDPLELLEV VD

« Hide

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Cross-references

Sequence databases

AE000512 Genomic DNA. Translation: AAD35434.1.
PIRB72389.
RefSeqNP_228159.1.

3D structure databases

HSSPHSSP built from PDB template 1SHK based on UniProtKB P10880.
ModBaseSearch...

Genome annotation databases

GeneID897304.
GenomeReviewsGene locus TM_0348 in contig AE000512_GR.
KEGGtma:TM0348.
NMPDRfig|243274.1.peg.343.
TIGRTM_0348.

Phylogenomic databases

OMAERIREIW.

Enzyme and pathway databases

BioCycTMAR243274:TM_0348-MON.
BRENDA2.7.1.71. 16699.
4.2.3.4. 16699.

Family and domain databases

HAMAPMF_00109. Fused.
[Tree]
MF_00110. Fused.
[Tree]
InterProIPR002658. DHQ_synth_AroB.
IPR016037. DHQ_synth_AroB_sub.
IPR000623. Shik_kinase.
[Graphical view]
PANTHERPTHR21090:SF1. DHQ_synth_AroB. 1 hit.
PfamPF01761. DHQ_synthase. 1 hit.
PF01202. SKI. 1 hit.
[Graphical view]
PRINTSPR01100. SHIKIMTKNASE.
TIGRFAMsTIGR01357. aroB. 1 hit.
PROSITEPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAROKB_THEMA
AccessionPrimary (citable) accession number: Q9WYI3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents