Q9WYI1 (AROE_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	TM_0346

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000136046
Regions
	Nucleotide binding	115 – 119	5	NADP By similarity
Sites
	Active site	63	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9WYI1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 64778D10CB07CE0E	FASTA	253	28,889
	10 20 30 40 50 60 MKFCIIGYPV RHSISPRLYN EYFKRAGMNH SYGMEEIPPE SFDTEIRRIL EEYDGFNATI 70 80 90 100 110 120 PHKERVMRYV EPSEDAQRIK AVNCVFRGKG YNTDWVGVVK SLEGVEVKEP VVVVGAGGAA 130 140 150 160 170 180 RAVIYALLQM GVKDIWVVNR TIERAKALDF PVKIFSLDQL DEVVKKAKSL FNTTSVGMKG 190 200 210 220 230 240 EELPVSDDSL KNLSLVYDVI YFDTPLVVKA RKLGVKHIIK GNLMFYYQAM ENLKIWGIYD 250 EEVFKEVFGE VLK

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000512 Genomic DNA. Translation: AAD35432.1.

PIR

H72388.

RefSeq

NP_228157.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3U62	X-ray	1.45	A	1-253	[»]

ProteinModelPortal

Q9WYI1.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

897302.

GenomeReviews

Gene locus TM_0346 in contig AE000512_GR.

KEGG

tma:TM0346.

NMPDR

fig|243274.1.peg.341.

PATRIC

23935573. VBITheMar51294_0351.

TIGR

TM_0346.

Phylogenomic databases

HOGENOM

HBG553408.

OMA

TDYYGFG.

PhylomeDB

Q9WYI1.

ProtClustDB

CLSK875100.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_0346-MONOMER.

Family and domain databases

HAMAP

MF_00222. Shikimate_DH_AroE.
[Tree]

InterPro

IPR016040. NAD(P)-bd_dom.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K00014.

Pfam

PF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AROE_THEMA

Accession

Primary (citable) accession number: Q9WYI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program