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Q9WYH8 (AROF_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase

EC=2.5.1.54
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene names
Name:aroF
Ordered Locus Names:TM_0343
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activity

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Cofactor

Divalent ions.

Enzyme regulation

Inhibited by L-phenylalanine and L-tyrosine.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the class-I DAHP synthase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 6.3 at 60 degrees Celsius.

Temperature dependence:

Optimum temperature is 90 degrees Celsius. Extremely thermostable.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Phospho-2-dehydro-3-deoxyheptonate aldolase
PRO_0000140847

Secondary structure

.................................................................... 338
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9WYH8 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: E9634B6704D3DF4D

FASTA33837,378
        10         20         30         40         50         60 
MIVVLKPGST EEDIRKVVKL AESYNLKCHI SKGQERTVIG IIGDDRYVVA DKFESLDCVE 

        70         80         90        100        110        120 
SVVRVLKPYK LVSREFHPED TVIDLGDVKI GNGYFTIIAG PCSVEGREML METAHFLSEL 

       130        140        150        160        170        180 
GVKVLRGGAY KPRTSPYSFQ GLGEKGLEYL REAADKYGMY VVTEALGEDD LPKVAEYADI 

       190        200        210        220        230        240 
IQIGARNAQN FRLLSKAGSY NKPVLLKRGF MNTIEEFLLS AEYIANSGNT KIILCERGIR 

       250        260        270        280        290        300 
TFEKATRNTL DISAVPIIRK ESHLPILVDP SHSGGRRDLV IPLSRAAIAV GAHGIIVEVH 

       310        320        330 
PEPEKALSDG KQSLDFELFK ELVQEMKKLA DALGVKVN 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD35429.1.
PIRE72388.
RefSeqNP_228154.1. NC_000853.1.
YP_007976691.1. NC_021214.1.
YP_008990684.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RZMX-ray2.20A/B1-338[»]
1VR6X-ray1.92A/B/C/D1-338[»]
3PG8X-ray2.00A/B71-338[»]
3PG9X-ray2.35A/B/C/D/E/F/G/H1-338[»]
4GRSX-ray3.00A/B/C/D1-309[»]
ProteinModelPortalQ9WYH8.
SMRQ9WYH8. Positions 1-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0343.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35429; AAD35429; TM_0343.
GeneID15493944.
18092455.
897299.
KEGGtma:TM0343.
tmm:Tmari_0341.
PATRIC23935567. VBITheMar51294_0348.

Phylogenomic databases

eggNOGCOG2876.
KOK03856.
OMALCKDISK.
OrthoDBEOG657JFQ.
ProtClustDBPRK08673.

Enzyme and pathway databases

UniPathwayUPA00053; UER00084.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006268. DAHP_syn_2.
[Graphical view]
PfamPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01361. DAHP_synth_Bsub. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9WYH8.

Entry information

Entry nameAROF_THEMA
AccessionPrimary (citable) accession number: Q9WYH8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: November 1, 1999
Last modified: March 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways