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Q9WYH8

- AROF_THEMA

UniProt

Q9WYH8 - AROF_THEMA

Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase

Gene

aroF

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

    Catalytic activityi

    Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

    Cofactori

    Divalent ions.

    Enzyme regulationi

    Inhibited by L-phenylalanine and L-tyrosine.

    pH dependencei

    Optimum pH is 6.3 at 60 degrees Celsius.

    Temperature dependencei

    Optimum temperature is 90 degrees Celsius. Extremely thermostable.

    Pathwayi

    GO - Molecular functioni

    1. 3-deoxy-7-phosphoheptulonate synthase activity Source: UniProtKB-EC
    2. aldehyde-lyase activity Source: InterPro

    GO - Biological processi

    1. aromatic amino acid family biosynthetic process Source: UniProtKB-KW
    2. chorismate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00053; UER00084.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)
    Alternative name(s):
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthase
    Phospho-2-keto-3-deoxyheptonate aldolase
    Gene namesi
    Name:aroF
    Ordered Locus Names:TM_0343
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338Phospho-2-dehydro-3-deoxyheptonate aldolasePRO_0000140847Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi243274.TM0343.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 55
    Helixi11 – 2313
    Beta strandi26 – 327
    Beta strandi37 – 4711
    Helixi50 – 545
    Beta strandi59 – 646
    Turni74 – 763
    Beta strandi82 – 843
    Beta strandi89 – 913
    Beta strandi94 – 1007
    Helixi107 – 11913
    Beta strandi124 – 1263
    Beta strandi128 – 1303
    Helixi144 – 15714
    Beta strandi160 – 1645
    Helixi168 – 1703
    Helixi171 – 1777
    Beta strandi179 – 1835
    Helixi185 – 1873
    Helixi191 – 1988
    Beta strandi204 – 2074
    Helixi214 – 22613
    Beta strandi232 – 2365
    Beta strandi245 – 2495
    Helixi254 – 2618
    Beta strandi262 – 2643
    Beta strandi266 – 2683
    Helixi270 – 2745
    Helixi277 – 2793
    Helixi280 – 29011
    Beta strandi293 – 2997
    Helixi303 – 3053
    Helixi310 – 3123
    Helixi316 – 33318
    Beta strandi336 – 3383

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RZMX-ray2.20A/B1-338[»]
    1VR6X-ray1.92A/B/C/D1-338[»]
    3PG8X-ray2.00A/B71-338[»]
    3PG9X-ray2.35A/B/C/D/E/F/G/H1-338[»]
    4GRSX-ray3.00A/B/C/D1-103[»]
    ProteinModelPortaliQ9WYH8.
    SMRiQ9WYH8. Positions 1-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WYH8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-I DAHP synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2876.
    KOiK03856.
    OMAiKPHISKG.
    OrthoDBiEOG657JFQ.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006218. DAHP1/KDSA.
    IPR006268. DAHP_syn_2.
    [Graphical view]
    PfamiPF00793. DAHP_synth_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01361. DAHP_synth_Bsub. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WYH8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIVVLKPGST EEDIRKVVKL AESYNLKCHI SKGQERTVIG IIGDDRYVVA    50
    DKFESLDCVE SVVRVLKPYK LVSREFHPED TVIDLGDVKI GNGYFTIIAG 100
    PCSVEGREML METAHFLSEL GVKVLRGGAY KPRTSPYSFQ GLGEKGLEYL 150
    REAADKYGMY VVTEALGEDD LPKVAEYADI IQIGARNAQN FRLLSKAGSY 200
    NKPVLLKRGF MNTIEEFLLS AEYIANSGNT KIILCERGIR TFEKATRNTL 250
    DISAVPIIRK ESHLPILVDP SHSGGRRDLV IPLSRAAIAV GAHGIIVEVH 300
    PEPEKALSDG KQSLDFELFK ELVQEMKKLA DALGVKVN 338
    Length:338
    Mass (Da):37,378
    Last modified:November 1, 1999 - v1
    Checksum:iE9634B6704D3DF4D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35429.1.
    PIRiE72388.
    RefSeqiNP_228154.1. NC_000853.1.
    YP_007976691.1. NC_021214.1.
    YP_008990684.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35429; AAD35429; TM_0343.
    GeneIDi897299.
    KEGGitma:TM0343.
    tmi:THEMA_03000.
    tmm:Tmari_0341.
    PATRICi23935567. VBITheMar51294_0348.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35429.1 .
    PIRi E72388.
    RefSeqi NP_228154.1. NC_000853.1.
    YP_007976691.1. NC_021214.1.
    YP_008990684.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RZM X-ray 2.20 A/B 1-338 [» ]
    1VR6 X-ray 1.92 A/B/C/D 1-338 [» ]
    3PG8 X-ray 2.00 A/B 71-338 [» ]
    3PG9 X-ray 2.35 A/B/C/D/E/F/G/H 1-338 [» ]
    4GRS X-ray 3.00 A/B/C/D 1-103 [» ]
    ProteinModelPortali Q9WYH8.
    SMRi Q9WYH8. Positions 1-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0343.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35429 ; AAD35429 ; TM_0343 .
    GeneIDi 897299.
    KEGGi tma:TM0343.
    tmi:THEMA_03000.
    tmm:Tmari_0341.
    PATRICi 23935567. VBITheMar51294_0348.

    Phylogenomic databases

    eggNOGi COG2876.
    KOi K03856.
    OMAi KPHISKG.
    OrthoDBi EOG657JFQ.

    Enzyme and pathway databases

    UniPathwayi UPA00053 ; UER00084 .

    Miscellaneous databases

    EvolutionaryTracei Q9WYH8.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006218. DAHP1/KDSA.
    IPR006268. DAHP_syn_2.
    [Graphical view ]
    Pfami PF00793. DAHP_synth_1. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01361. DAHP_synth_Bsub. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase: the ancestral eubacterial DAHP synthase?"
      Wu J., Howe D.L., Woodard R.W.
      J. Biol. Chem. 278:27525-27531(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiAROF_THEMA
    AccessioniPrimary (citable) accession number: Q9WYH8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2003
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3