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Q9WYH8

- AROF_THEMA

UniProt

Q9WYH8 - AROF_THEMA

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Protein
Phospho-2-dehydro-3-deoxyheptonate aldolase
Gene
aroF, TM_0343
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Cofactori

Divalent ions.

Enzyme regulationi

Inhibited by L-phenylalanine and L-tyrosine.

pH dependencei

Optimum pH is 6.3 at 60 degrees Celsius.

Temperature dependencei

Optimum temperature is 90 degrees Celsius. Extremely thermostable.

Pathwayi

GO - Molecular functioni

  1. 3-deoxy-7-phosphoheptulonate synthase activity Source: UniProtKB-EC
  2. aldehyde-lyase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-KW
  2. chorismate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroF
Ordered Locus Names:TM_0343
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Phospho-2-dehydro-3-deoxyheptonate aldolase
PRO_0000140847Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi243274.TM0343.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 55
Helixi11 – 2313
Beta strandi26 – 327
Beta strandi37 – 4711
Helixi50 – 545
Beta strandi59 – 646
Turni74 – 763
Beta strandi82 – 843
Beta strandi89 – 913
Beta strandi94 – 1007
Helixi107 – 11913
Beta strandi124 – 1263
Beta strandi128 – 1303
Helixi144 – 15714
Beta strandi160 – 1645
Helixi168 – 1703
Helixi171 – 1777
Beta strandi179 – 1835
Helixi185 – 1873
Helixi191 – 1988
Beta strandi204 – 2074
Helixi214 – 22613
Beta strandi232 – 2365
Beta strandi245 – 2495
Helixi254 – 2618
Beta strandi262 – 2643
Beta strandi266 – 2683
Helixi270 – 2745
Helixi277 – 2793
Helixi280 – 29011
Beta strandi293 – 2997
Helixi303 – 3053
Helixi310 – 3123
Helixi316 – 33318
Beta strandi336 – 3383

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RZMX-ray2.20A/B1-338[»]
1VR6X-ray1.92A/B/C/D1-338[»]
3PG8X-ray2.00A/B71-338[»]
3PG9X-ray2.35A/B/C/D/E/F/G/H1-338[»]
4GRSX-ray3.00A/B/C/D1-103[»]
ProteinModelPortaliQ9WYH8.
SMRiQ9WYH8. Positions 1-338.

Miscellaneous databases

EvolutionaryTraceiQ9WYH8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2876.
KOiK03856.
OMAiKPHISKG.
OrthoDBiEOG657JFQ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006268. DAHP_syn_2.
[Graphical view]
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01361. DAHP_synth_Bsub. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WYH8-1 [UniParc]FASTAAdd to Basket

« Hide

MIVVLKPGST EEDIRKVVKL AESYNLKCHI SKGQERTVIG IIGDDRYVVA    50
DKFESLDCVE SVVRVLKPYK LVSREFHPED TVIDLGDVKI GNGYFTIIAG 100
PCSVEGREML METAHFLSEL GVKVLRGGAY KPRTSPYSFQ GLGEKGLEYL 150
REAADKYGMY VVTEALGEDD LPKVAEYADI IQIGARNAQN FRLLSKAGSY 200
NKPVLLKRGF MNTIEEFLLS AEYIANSGNT KIILCERGIR TFEKATRNTL 250
DISAVPIIRK ESHLPILVDP SHSGGRRDLV IPLSRAAIAV GAHGIIVEVH 300
PEPEKALSDG KQSLDFELFK ELVQEMKKLA DALGVKVN 338
Length:338
Mass (Da):37,378
Last modified:November 1, 1999 - v1
Checksum:iE9634B6704D3DF4D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35429.1.
PIRiE72388.
RefSeqiNP_228154.1. NC_000853.1.
YP_007976691.1. NC_021214.1.
YP_008990684.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35429; AAD35429; TM_0343.
GeneIDi897299.
KEGGitma:TM0343.
tmi:THEMA_03000.
tmm:Tmari_0341.
PATRICi23935567. VBITheMar51294_0348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000512 Genomic DNA. Translation: AAD35429.1 .
PIRi E72388.
RefSeqi NP_228154.1. NC_000853.1.
YP_007976691.1. NC_021214.1.
YP_008990684.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RZM X-ray 2.20 A/B 1-338 [» ]
1VR6 X-ray 1.92 A/B/C/D 1-338 [» ]
3PG8 X-ray 2.00 A/B 71-338 [» ]
3PG9 X-ray 2.35 A/B/C/D/E/F/G/H 1-338 [» ]
4GRS X-ray 3.00 A/B/C/D 1-103 [» ]
ProteinModelPortali Q9WYH8.
SMRi Q9WYH8. Positions 1-338.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243274.TM0343.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD35429 ; AAD35429 ; TM_0343 .
GeneIDi 897299.
KEGGi tma:TM0343.
tmi:THEMA_03000.
tmm:Tmari_0341.
PATRICi 23935567. VBITheMar51294_0348.

Phylogenomic databases

eggNOGi COG2876.
KOi K03856.
OMAi KPHISKG.
OrthoDBi EOG657JFQ.

Enzyme and pathway databases

UniPathwayi UPA00053 ; UER00084 .

Miscellaneous databases

EvolutionaryTracei Q9WYH8.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006268. DAHP_syn_2.
[Graphical view ]
Pfami PF00793. DAHP_synth_1. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01361. DAHP_synth_Bsub. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase: the ancestral eubacterial DAHP synthase?"
    Wu J., Howe D.L., Woodard R.W.
    J. Biol. Chem. 278:27525-27531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiAROF_THEMA
AccessioniPrimary (citable) accession number: Q9WYH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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